[1]张华清,汪隆海a,张求霞a,等.改良ELISA法定量测定血清中段心房利钠肽原的评价及临床应用[J].现代检验医学杂志,2018,33(04):51-54，58.[doi:10.3969/j.issn.1671-7414.2018.04.013]
　ZHANG Hua-qing,WANG Long-haia,ZHANG Qiu-xiaa,et al.Evaluation and Clinical Application of Modified Enzyme-Linked Immunosorbent Assay for Quantitative Determination of Midregional Pro-Atrial Natriuretic Peptide in Serum[J].Journal of Modern Laboratory Medicine,2018,33(04):51-54，58.[doi:10.3969/j.issn.1671-7414.2018.04.013]
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改良ELISA法定量测定血清中段心房利钠肽原的评价及临床应用()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第33卷
期数:
2018年04期
页码:
51-54，58
栏目:
论著
出版日期:
2018-08-30

文章信息/Info

Title:
Evaluation and Clinical Application of Modified Enzyme-Linked Immunosorbent Assay for Quantitative Determination of Midregional Pro-Atrial Natriuretic Peptide in Serum
文章编号:
1671-7414(2018)04-051-05
作者:
张华清1; 汪隆海2a; 张求霞2a; 胡 勇2b
1.中南大学湘雅公共卫生学院,长沙 410013; 2.宋庆龄爱心医院a.检验科; b.心内科,安徽巢湖 238000
Author(s):
ZHANG Hua-qing1; WANG Long-hai2a; ZHANG Qiu-xia2a; HU Yong2b
1.Xiangya School of Public Health,Cental South University,Changsha 410013,China; 2a.Department of Clinical Laboratory; 2b.Department of Cardiology,Song Qingling Caring Hospital,Anhui Chaohu 238000,China
关键词:
中段心房利钠肽原;  酶联免疫吸附测定;  高血压;  急性心肌梗死;  心功能衰竭;  获得性肺炎
分类号:
R446.61
DOI:
10.3969/j.issn.1671-7414.2018.04.013
文献标志码:
A
摘要:
目的 建立血清中段心房利钠肽原(MR-proANP)的改良ELISA法定量测定,并探讨其应用价值。方法 用定量ELISA法测定患者与健康者的血清MR-proANP,并运用IBM SPSS Statistics 23.0软件对结果进行统计处理。结果 该法检出限为1.002 pmol/L,平均回收率为98.33%,批内CV为2.64%,批间CV为4.95%。健康者与患者的血清MR-proANP值通过对数转换,并用均数±标准差((-overx)±s)表示; 男性健康者120例、高血压41例、急性心肌梗死(AMI)23例、心功能衰竭37例及获得性肺炎患者41例的血清MR-proANP值分别为1.25±0.48,2.45±0.34,3.10±0.41,2.73±0.51和2.43±0.34 pmol/L,差异具有统计学意义(F=166.487,P=0.000); 女性健康者120例、高血压40例、AMI 22例、心功能衰竭35例及获得性肺炎患者39例的血清MR-proANP值分别为1.39±0.48,2.73±0.59,3.47±0.69,3.06±0.75和2.70±0.58 pmol/L,差异也具有统计学意义(F=122.412,P=0.000)。结论 该法灵敏、特异和准确。血清MR-proANP定量测定是诊断高血压、AMI,心功能衰竭和获得性肺炎的较好实验指标。
Abstract:
Abstract:Objective To establish enzyme-linked immunosorbent assay(ELISA)for the quantitative determination of midregional pro-atrialnatriuretic peptide(MR-pro ANP)in serum and to investigate its clinical application.Methods Serum MR-pro ANP concentrations of patients and of normal controls were measured by ELISA.IBM SPSS Statistics 23.0 software was employed for statistical analysis in this study.Results The limit of detection(LOD)was 1.002 pmol/L.The average rate of recovery was 98.33%.The intraassay CV was 2.64% and interassay CV was 4.95%,respectively.The serum MR-pro ANP value of healthy person and patientwere transformed through logarithmic transformation,expressed in Mean±SD.Themale value of healthy persons(n=120),hypertension(n=41),AMI(n=23),heart failure(n=37)and acquired pneumonia(n=41)were 1.25±0.48,2.45±0.34,3.10±0.41,2.73±0.51 and 2.43±0.34 pmol/L,respectively.Significant differences were observed in them(F=166.487,P=0.000).Thefemale value were 1.39±0.48,2.73±0.59,3.47±0.69,3.06±0.75 and 2.70±0.58 pmol/L,respectively.Significant differences were observed in them(F=122.4,P=0.000).Conclusion This method is sensitive,specific and accurate.Quantitative detection of MR-pro ANP is now betterlaboratory test for patients of hypertension,of AMI,of heart failure and of acquired pneumonia.

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备注/Memo

备注/Memo:
作者简介:张华清(1999-),男,本科在读,从事临床医学研究,E-mail:1362398363@qq.com。
更新日期/Last Update: 2018-08-16