[1]李双玉,董 磊,解金辉,等.一例罕见AB亚型的分子生物学研究分析[J].现代检验医学杂志,2018,33(04):70-72.[doi:10.3969/j.issn.1671-7414.2018.04.018]
　LI Shuang-yu,DONG Lei,XIE Jin-hui,et al.Study on the Molecular Mechanism of a Case of Rare AB Subtype[J].Journal of Modern Laboratory Medicine,2018,33(04):70-72.[doi:10.3969/j.issn.1671-7414.2018.04.018]
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一例罕见AB亚型的分子生物学研究分析()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第33卷
期数:
2018年04期
页码:
70-72
栏目:
论著
出版日期:
2018-08-30

文章信息/Info

Title:
Study on the Molecular Mechanism of a Case of Rare AB Subtype
文章编号:
1671-7414(2018)04-070-03
作者:
李双玉; 董 磊; 解金辉; 赵 瑛
天津市血液中心,天津 300110
Author(s):
LI Shuang-yu; DONG Lei; XIE Jin-hui; ZHAO Ying
Tianjin Blood Center,Tianjin 300110,China
关键词:
ABO血型;  亚型;  分子生物学技术
分类号:
R457.11
DOI:
10.3969/j.issn.1671-7414.2018.04.018
文献标志码:
A
摘要:
目的 分析研究一名献血者ABO血型形成的分子生物学机制。方法 用血清学方法检测ABO和H抗原,应用聚合酶链式反应(polymerase chain reation,PCR)检测其基因型,再对ABO基因第6,7外显子进行直接测序。结果 该献血员血清学检测为AB亚型,PCR结果为A2/Bw12,直接测序结果为A205/Bw12。结论 该献血员血型是一例比较罕见的AB双侧亚型。为保障输血安全,在该献血员不含抗体的情况下,其血液可以输注给配血相合的AB型患者。
Abstract:
Abstract:Objective To explore the molecular mechanism ofa blood donor's ABO blood group.Methods The serological test was applied to test the ABO and H antigens of the blood donor.The polymerase chain reation(PCR)was applied to exam the ABO genotype,and the direct sequencing was applied to test the exon6 and exon7 sequence of the blood donor'sABO gene.Results The results of the serological test indicated that the donor's ABO blood was AB subtype.The A2/Bw12 genotype was acquired with PCR and the A205/Bw12 genotpye was acquired with the direct sequencing.Conclusion The donor's blood type is a rare AB subtype with both A and B are subtypes.

参考文献/References:

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备注/Memo

备注/Memo:
[13] 王 伟,胡豫杰,徐 进,等.鼠伤寒沙门菌婴幼儿分离株耐药基因及毒力基因研究[J].中国食品卫生杂志,2016,28(5):567-575. Wang W,Hu YJ,Xu J,et al.Analysis of antibiotic resistance genes and virulence genes in Salmonella typhimurium isolated from fecal samples of children under 5 year old by whole genome sequencing[J].Chinese Journal of Food Hygiene,2016,28(5):567-575.
更新日期/Last Update: 2018-08-16