[1]张 丽,齐 军,吴宗勇,等.耐碳青霉烯类肺炎克雷伯菌临床分离株中喹诺酮类及16SrRNA甲基化酶基因的检测[J].现代检验医学杂志,2018,33(05):27-30.[doi:10.3969/j.issn.1671-7414.2018.05.008]
　ZHANG Li,QI Jun,WU Zong-yong,et al.Detection of Quinolone and 16SrRNA Methyltransferasegene of Clinical Isolated Carbapenem-Resistant Klebsiella Pneumoniae[J].Journal of Modern Laboratory Medicine,2018,33(05):27-30.[doi:10.3969/j.issn.1671-7414.2018.05.008]
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耐碳青霉烯类肺炎克雷伯菌临床分离株中喹诺酮类及16SrRNA甲基化酶基因的检测()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第33卷
期数:
2018年05期
页码:
27-30
栏目:
论著
出版日期:
2018-11-18

文章信息/Info

Title:
Detection of Quinolone and 16SrRNA Methyltransferasegene of Clinical Isolated Carbapenem-Resistant Klebsiella Pneumoniae
文章编号:
1671-7414(2018)05-027-04
作者:
张 丽1; 齐 军2; 吴宗勇2; 张晓煜1; 吴卫星1
1.国家癌症中心/国家肿瘤临床医学研究中心/中国医学科学院肿瘤医院北京协和医学院肿瘤医院深圳医院检验科,广东深圳 518116; 2.国家癌症中心/国家肿瘤临床医学研究中心/中国医学科学院北京协和医学院肿瘤医院检验科,北京100029
Author(s):
ZHANG Li1; QI Jun2; WU Zong-yong2; ZHANG Xiao-yu1; WU Wei-xing1
1.Department of Laboratory Medicine,National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital & Shenzhen Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Shenzhen 518116,China; 2.Department of Laboratory Medicine,National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100021,China
关键词:
耐碳青霉烯类肺炎克雷伯菌;  喹诺酮类耐药基因;  16SrRNA甲基化酶基因;  耐药性
分类号:
R378.996; Q781
DOI:
10.3969/j.issn.1671-7414.2018.05.008
文献标志码:
A
摘要:
目的 了解临床分离株中耐碳青霉烯肺炎克雷伯菌(CRKP)的氟喹诺酮类耐药基因及16SrRNA甲基化酶基因存在情况和药敏特点。方法 收集临床标本中分离的6株CRKP,采用VITEK 2 compact全自动微生物鉴定和药敏系统鉴定细菌及药敏试验,采用聚合酶链反应(PCR)扩增氟喹诺酮类耐药基因和16SrRNA甲基化酶基因。结果 6株CRKP临床分离株呈现多药耐药特点,对环丙沙星和左氧氟沙星的耐药率分别是83.3%和83.3%,对庆大霉素、阿米卡星和妥布霉素的耐药率分别是66.7%,50%和66.7%。检出喹诺酮类耐药相关基因qnrB,qnrS和qnrD,基因阳性率分别为50%,50%和33.3%,未检出qnrA,qnrC和qepA基因。检出16SrRNA甲基化酶耐药相关基因armA,基因阳性率为50%,未检出rmtA,rmtB,rmtC和npmA基因。结论 6株CRKP对喹诺酮类和氨基糖类抗生素耐药严重,其耐药性与喹诺酮类耐药基因及16SrRNA甲基化酶基因高度相关,其耐药基因主要型别分别是qnrB,qnrS,qnrD 和armA。
Abstract:
Objective To understand the presence of quinolone resistance gene and 16SrRNA methylation enzyme gene and the characteristics of drug sensitivity in the clinical isolated carbapenem-resistant Klebsiella pneumoniae(CRKP).Methods 6 strains of CRKP isolated from clinical specimens were collected,and bacteria and drug sensitivity tests were identified by VITEK 2 compact automatic microbial identification and drug sensitivity system,and polymerase chain reaction(PCR)was used to amplify the quinolone resistance gene and 16SrRNA methylase gene.Results 6 strains of CRKP clinical isolates showed multidrug resistance,and the resistance rates for ciprofloxacin and levofloxacin were 83.3% and 83.3% respectively.The resistance rates for gentamicin,amikacin and tobramycin were 66.7%,50% and 66.7%,respectively.The positive rates of qnrB,qnrS and qnrD genes were 50%,50% and 33.3%,respectively.The negative rates of qnrA,qnrC and qepA were found.The 16SrRNA methylase resistance related genes were detected.The positive rate of armA gene was 50%,and rmtA,rmtB,rmtC and npmA genes were negative.Conclusion 6 strains of CRKP were resistant to quinolones and aminoglycos,and their resistance was highly correlated with quinoloneresistance genes and 16SrRNA methylase genes.The main types of drug resistancegenes were qnrB,qnrS,qnrD and armA.

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备注/Memo

备注/Memo:
基金项目:深圳市医疗卫生三名工程项目资助,项目编号:SZSM201812062。
作者简介:张 丽(1977-),女,医学硕士,副主任技师,主要工作和研究方向:微生物学检验及耐药机制研究,E-mail:zldh33@163.com。
更新日期/Last Update: 2018-09-30