[1]林帝金,刘纯岳,方俊粤,等.乳腺肿瘤患者外周血中T淋巴细胞表面活化分子和Tregs的表达研究[J].现代检验医学杂志,2018,33(05):31-34+37.[doi:10.3969/j.issn.1671-7414.2018.05.009]
　LIN Di-jin,LIU Chun-yue,FANG Jun-yue,et al.Expression of T Lymphocyte Surface Activating Molecules and Tregs in Peripheral Blood of Patients with Breast Neoplass[J].Journal of Modern Laboratory Medicine,2018,33(05):31-34+37.[doi:10.3969/j.issn.1671-7414.2018.05.009]
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乳腺肿瘤患者外周血中T淋巴细胞表面活化分子和Tregs的表达研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第33卷
期数:
2018年05期
页码:
31-34+37
栏目:
论著
出版日期:
2018-11-18

文章信息/Info

Title:
Expression of T Lymphocyte Surface Activating Molecules and Tregs in Peripheral Blood of Patients with Breast Neoplass
文章编号:
1671-7414(2018)05-031-05
作者:
林帝金; 刘纯岳; 方俊粤; 黄洁珊; 林向华
中山大学孙逸仙纪念医院检验科,广州 510120
Author(s):
LIN Di-jin; LIU Chun-yue; FANG Jun-yue; HUANG Jie-shan; LIN Xiang-hua
Department of Clinical Laboratory, Sun Yat-Sen Memorial Hospital,Sun Yat-Sen University,Guangzhou 510120,China
关键词:
乳腺肿瘤;  CD25;  CD69;  HLA-DR;  调节性T细胞
分类号:
R737.9; R730.43
DOI:
10.3969/j.issn.1671-7414.2018.05.009
文献标志码:
A
摘要:
目的 探讨T淋巴细胞表面活化分子和调节性T细胞(regulatory T cells,Tregs )在乳腺肿瘤患者中的表达及其临床意义。方法 选取2016～2017年在中山大学孙逸仙纪念医院进行诊治的245例乳腺肿瘤患者(包括恶性乳腺肿瘤139例和良性乳腺肿瘤106例)为研究对象,采用流式细胞术检测T淋巴细胞表面活化分子CD25,CD69,HLA-DR以及Tregs的表达。结果 与健康对照组比较,乳腺肿瘤患者的CD3+CD25+T淋巴细胞,CD3+HLA-DR+T淋巴细胞和CD8+HLA-DR+T淋巴细胞均明显上升,且恶性肿瘤患者较良性肿瘤患者升高更显著。此外,Tregs在恶性肿瘤患者的百分比较良性肿瘤患者以及健康对照组均升高,恶性肿瘤患者的CD4+T淋巴细胞百分比较良性肿瘤患者升高显著,以上差异均有统计学意义(P<0.05)。T淋巴细胞表面活化分子的表达在肿瘤分期及淋巴结是否转移中差异无统计学意义(P>0.05)。结论 乳腺肿瘤患者外周血T淋巴细胞表面活化分子CD25和HLA-DR表达明显升高,密切监测可辅助临床对乳腺肿瘤患者的病情和疗效进行判断。
Abstract:
Objective To investigate the expression and clinical significance of T lymphocyte surface activator and Tregs in breast neoplasms patients.Methods 245 patients(including 139 cases ofmalignant tumors and 106 cases of benign tumors)with breast neoplasms were selected as the research object to investigate the expression of T lymphocyte activating molecules CD25,CD69,HLA-DR and regulatory T cell(Tregs)by flow cytometry.Results Compared with healthy controls,CD3+CD25+T lymphocytes,CD3+HLA-DR+T lymphocytes and CD8+HLA-DR+T lymphocytesincreased significantly in breast neoplasms patients,and those in malignant tumors increased more significantly than those in benign tumors.In addition,theexpression level of Tregs in malignant tumor patients was higher than that in benign tumor patients and healthy controls.The CD4+T lymphocytes in malignant tumor patients were significantly higher than those in benign tumor patients(P<0.05).The expression level of T lymphocyte activation molecules were influenced neither by the tumor staging nor by lymph node metastasis(P>0.05).Conclusion The expression levels of CD25 and HLA-DR on thesurface of T lymphocytes in breast neoplasms patients were significantly increased.Close monitoring T lymphocytes surface activator could help breast neoplasms patients in clinical diagnosis,judging the condition and efficacy monitoring.

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备注/Memo

备注/Memo:
作者简介:林帝金(1986-),男,学士,技师,研究方向:细胞免疫学,Tel:18312016321,E-mail:282411099@qq.com。
通讯作者:林向华,E-mail:13826068632@139.com。
更新日期/Last Update: 2018-09-30