[1]朱媛媛a,顾万建b,徐 天b,等.男性不育患者精浆miR-551b水平变化及其临床价值[J].现代检验医学杂志,2018,33(06):1-4+8.[doi:10.3969/j.issn.1671-7414.2018.06.001]
　ZHU Yuan-yuana,GU Wan-jianb,XU Tianb,et al.Clinical Value of Seminal Plasma miR-551b in the Diagnosis of Male Infertility[J].Journal of Modern Laboratory Medicine,2018,33(06):1-4+8.[doi:10.3969/j.issn.1671-7414.2018.06.001]
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男性不育患者精浆miR-551b水平变化及其临床价值()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第33卷
期数:
2018年06期
页码:
1-4+8
栏目:
论著
出版日期:
2018-12-24

文章信息/Info

Title:
Clinical Value of Seminal Plasma miR-551b in the Diagnosis of Male Infertility
文章编号:
1671-7414(2018)06-001-05
作者:
朱媛媛1a; 顾万建1b; 徐 天1b; 邵 永2a; 王 成2b; 张春妮2b
1.江苏省中医院a.科技处; b.检验科,南京 210029; 2.南京总医院a.生殖医学中心; b.检验科,南京 210002
Author(s):
ZHU Yuan-yuan1a; GU Wan-jian1b; XU Tian1b; SHAO Yong2a; WANG Cheng2b; ZHANG Chun-ni2b
1a.Department of Science and Technology; 1b.Departmentof Clinical Laboratory,Jiangsu Province Hospital of TCM,Nanjing 210029,China; 2a.Reproductive Medical Center; 2b.Department of Clinical Laboratory, Nanjing General Hospital,Nanjing 210002,China
关键词:
微小核糖核酸-551b;  男性不育症;  精浆;  弱精症;  无精症;  分子标志物
分类号:
R698.2; R446.19
DOI:
10.3969/j.issn.1671-7414.2018.06.001
文献标志码:
A
摘要:
目的 检测和分析男性不育症患者精浆miR-551b水平变化,探讨其作为男性不育症辅助分子标志物的可能性。方法 选取2008年11月～2015年3月在南京总医院生殖医学中心和江苏省中医院男科门诊确诊的92例男性不育症患者及同期招募的34例年龄匹配正常生育男性精液标本,记录患者及对照临床资料,测定患者及对照精液参数包括精子密度、精子活率、a级精子比例、a+b级精子比例及部分精浆生化指标α-糖苷、酸性磷酸酶、肉毒碱、果糖水平。实时荧光定量PCR技术(quantitative real-time PCR,qRT-PCR)检测和比较弱精症、非梗阻性无精症患者及正常生育男性精浆中miR-551b含量变化,统计分析精浆miR-551b临床价值及与精液参数、精浆生化指标相关性。结果 qRT-PCR结果显示,正常生育组精浆miR-551b水平为20.63(9.59,37.83)fmol/L,弱精症患者为62.29(25.22,101.43)fmol/L,较正常生育组显著升高(U=297.00); 无精症患者精浆miR-551b水平为4.70(2.41,13.71)fmol/L,较正常生育组明显降低(U=356.00),差异均具有统计学意义(P<0.001)。受试者工作特征曲线(receiver operating characteristic curve,ROC)分析显示,精浆miR-551b区分弱精症、无精症患者与正常生育组的ROC曲线下面积(AUC ROC)分别为0.810(95%CI 0.718～0.902)和0.772(95%CI 0.667～0.878); 鉴别弱精症和无精症患者的AUC ROC为0.932(95%CI 0.885～0.979)。Spearman秩相关性分析显示,男性不育患者精浆miR-551b水平与精子密度(r=0.735)、精子活率(r=0.643)、a级精子比例(r=0.672)、a+b级精子比例(r=0.682)及α-糖苷(r=0.375)呈正相关,差异均具有统计学意义(均P<0.05)。逐步多元线性回归结果显示,男性不育症患者精浆miR-551b水平与精子密度呈显著独立相关(β=0.618,P<0.001,校正r2=0.368)。逻辑回归分析显示,精浆miR-551b是弱精症[OR = 24.889(95%CI 5.302～116.843),P<0.001]和无精症[OR=6.303(95%CI 1.316～30.179),P=0.021]的潜在危险因素。结论 男性不育症患者精浆miR-551b水平与正常生育男性存在明显差异,且与精子密度和活力密切相关,有潜力成为男性不育患者辅助诊断和鉴别诊断的新型分子标志物。
Abstract:
Objective To investigate the alteration patternof seminal plasma miR-551b in infertile men and further evaluate the diagnostic potential of seminal plasma miR-551b as an auxiliary molecular marker for male infertility.Methods Semen samples were collected from92 infertile men and 34 age-matched fertile controls in Nanjing General Hospital and Jiangsu Province Hospital of TCM between November 2008 and March 2015.Semen parameters including sperm density,motility rate,the ratio of grade a spermatozoon and grade a+b spermatozoon,as well as seminal plasma biochemical indexes such as alpha-glucosidase,acid phosphatase,carnitine and fructose were assessed.Quantitive Real-time PCR(qRT-PCR)assays were used to determine the concentrations of seminal plasma miR-551b in the patients with asthenozoospermia,non-obstructive azoospermia and fertile controls.Statistical analyses were performed to evaluate the clinical value of seminal plasma miR-551b for male infertility as well as to assess the clinical association between the levels of seminal plasma miR-551b and other semen and seminal plasma parameters.Results The seminal plasma levels of miR-551b in the asthenozoospermia group[62.29(25.22,101.43)fmol/L] were significantly higher than that in the fertile control[20.63(9.59,37.83)fmol/L](U=297.00,P<0.001),but with markedly lower concentration in the non-obstructive azoospermia group [4.70(2.41, 13.71)fmol/L] as compared with the controls(U=356.00,P<0.001).ROC curve analysis showed that AUCs of miR-551b for discriminating asthenozoospermia and non-obstructive azoospermia were 0.810(95%CI 0.718～0.902)and 0.772(95%CI 0.667～0.878),respectively,and that the AUCs for differentiating asthenozoospermia and non-obstructive azoospermia was 0.932(95%CI 0.885～0.979).Spearman rank correlations analyses showed that the levels ofseminal plasma miR-551b were significantly associated with sperm density(r=0.735),motility rate(r=0.643),the ratio of grade a spermatozoon(r=0.672),the ratio of grade a+b spermatozoon(r=0.682)and alpha-glucosidase(r=0.375),all with statistical significance(all P<0.05)in male infertility.Multiple linear regression analyses revealed that seminal plasmamiR-551b was independently associated with sperm density(β=0.618,P<0.001,adjusted(r2=0.368)after adjust other co-factors.Furthermore,logistic regression analyses demonstrated that seminal plasma miR-551b was a potential risk factor for asthenozoospermia [OR=24.889(95%CI 5.302～116.843),P<0.001] and non-obstructive azoospermia [OR=6.303(95%CI 1.316～30.179),P=0.021].Conclusion The levels of seminal plasma miR-551b in patients with male infertility were significantly different from that in fertile men,and closely associated with sperm density and motility,which indicating that miR-551b may be a novel auxiliary molecular marker for diagnosing and differentiating male infertility.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(81401257); 江苏省中医院院级创新发展基金(Y2018CX07)。
作者简介:朱媛媛(1989-),女,在读硕士研究生,实习研究员,从事微小核糖核酸临床价值及功能研究,E-mail: zyta1010@126.com。
通讯作者:王 成(1985-),男,博士,主管技师,E-mail: wangchengnju@163.com。
张春妮(1963-),女,博士,主任技师,博士生导师,E-mail: zchunni27@hotmail.com。
更新日期/Last Update: 2018-11-30