[1]郭宜晨,鲁亚静,钟伟传,等.2型糖尿病患者外周血miRNA表达谱差异研究[J].现代检验医学杂志,2018,33(06):5-8.[doi:10.3969/j.issn.1671-7414.2018.06.002]
　GUO Yi-chen,LU Ya-jing,ZHONG Wei-chuan,et al.Study on the Difference of Serum miRNA Expression Profile in Peripheral Blood of Patients with Type 2 Diabetes Mellitus[J].Journal of Modern Laboratory Medicine,2018,33(06):5-8.[doi:10.3969/j.issn.1671-7414.2018.06.002]
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2型糖尿病患者外周血miRNA表达谱差异研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第33卷
期数:
2018年06期
页码:
5-8
栏目:
论著
出版日期:
2018-12-24

文章信息/Info

Title:
Study on the Difference of Serum miRNA Expression Profile in Peripheral Blood of Patients with Type 2 Diabetes Mellitus
文章编号:
1671-7414(2018)06-005-04
作者:
郭宜晨1; 鲁亚静2; 钟伟传3; 陈雪艳3; 郭广洲3
1.北京大学深圳医院,广东深圳 518036; 2.深圳市梓健生物科技有限公司,广东深圳 518000; 3.深圳市龙华区人民医院,广东深圳 518109
Author(s):
GUO Yi-chen1; LU Ya-jing2; ZHONG Wei-chuan3; CHEN Xue-yan3; GUO Guang-zhou3
1.Shenzhen Hospital of Peking University,Guangdong Shenzhen 518036,China; 2.Shenzhen Zijian Biotechnology CO.LTD,Guangdong Shenzhen 518000,China; 3.the People's Hospital of Longhua District,Guangdong Shenzhen518109,China
关键词:
2型糖尿病;  miRNA;  GeneChipTM微小RNA 4.0 Array;  胰岛素抵抗;  KEGG
分类号:
R587.1; Q786
DOI:
10.3969/j.issn.1671-7414.2018.06.002
文献标志码:
A
摘要:
目的 对2型糖尿病(type 2 diabetes mellitus,T2DM)患者外周血血清miRNA差异表达分析,为筛选出T2DM发生发展有关的miRNA分子标记物奠定基础,从而为T2DM预防、早期诊断和治疗方案提供指导。方法 采用GeneChipTM miRNA 4.0 Array筛选3组受试者外周血血清(T2DM组、T1DM组、对照组)的差异miRNA,并对差异miRNA进行靶基因预测和靶基因信号通路富集分析(KEGG)。结果 T2DM组分别与对照组,T1DM组相比,hsa-miR-505-3p和hsa-miR-548an均上调,hsa-miR-296-3p,hsa-miR-3160-5p,hsa-miR-33a-5p,hsa-miR-572和hsa-miR-96-5p均下调。通过靶基因预测和Pathway分析,筛选出5个miRNA(hsa-miR-505-3p,hsa-miR-548an,hsa-miR-296-3p,hsa-miR-3160-5p,hsa-miR-33a-5p,hsa-miR-572和hsa-miR-96-5p)、7个基因(MTOR,SLC2A2,PRKCE,IRS2,IRS1,MAPK8,MAPK10)可能与2型糖尿病胰岛素抵抗相关。结论 该研究筛选出的5个miRNA和7个基因,可为2型糖尿病胰岛素抵抗的研究提供理论基础。
Abstract:
Objective Through analyzing the differential expression of serum miRNA in peripheral blood of type 2 diabetes mellitus(T2DM)patients to lay a foundation for screening out miRNA molecular markers related tothe occurrence and development of T2DM,so as to provide guidance for prevention,early diagnosis and treatment of T2DM.Methods Used GeneChipTM miRNA4.0 Array to screen differential miRNA expression of threegroups(T2DM group,the T1DM group,control group)peripheral blood serum,thenpredicted target gene,analyzed the target genes by KEGG pathway analysis.Results Compared with the control group and the T1DM group,both hsa-miR-505-3p and hsa-miR-548an were up-regulated in the T2DM group,while hsa-miR-296-3p,hsa-miR-3160-5p,hsa-miR-33a-5p,hsa-miR-572 andhsa-miR-96-5p were down-regulated.Through target gene prediction and pathway analysis,5 miRNA(hsa-mir-505-3p,hsa-mir-548an,hsa-mir-3160-5p,hsa-miR-96-5p,hsa-mir-33a-5p)and 7 genes(MTOR,SLC2A2,PRKCE,IRS2,IRS1,MAPK8,MAPK10)were screened associated with insulin resistance in type 2 diabetes possibly.Conclusion Five miRNAs and seven genes were screened out,can provide a theoretical basis for the study of insulin resistance in type 2 diabetes.

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备注/Memo

备注/Memo:
基金项目:深圳市龙华区科技创新资金项目,深龙华经促科计字(2017)2号,项目编号:20160831A1030200。
作者简介:郭宜晨(1989-),女,硕士,医师,主要研究方向:糖尿病发病机制及慢性并发症防治,E-mail:13692186898@qq.com。
通讯作者:郭广洲,主任检验师,从事医学免疫学和医学检验工作及研究,E-mail:13692186898@163.com。
更新日期/Last Update: 2018-11-30