[1]王菊惠a,袁宝军a,李超a,等.1976年中国唐山地震生存截瘫患者淋巴细胞亚群水平检测研究[J].现代检验医学杂志,2019,34(01):38-40,46.[doi:10.3969/j.issn.1671-7414.2019.01.010]
　WANG Ju-huia,YUAN Bao-juna,LI Chao a,et al.Detection of Lymphocyte Subsets in Patients with Paraplegia after the 1976 Tangshan Earthquake in China[J].Journal of Modern Laboratory Medicine,2019,34(01):38-40,46.[doi:10.3969/j.issn.1671-7414.2019.01.010]
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1976年中国唐山地震生存截瘫患者淋巴细胞亚群水平检测研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第34卷
期数:
2019年01期
页码:
38-40,46
栏目:
论著
出版日期:
2019-02-28

文章信息/Info

Title:
Detection of Lymphocyte Subsets in Patients with Paraplegia after the 1976 Tangshan Earthquake in China
文章编号:
1671-7414( 2019)01-038-04
作者:
王菊惠1a; 袁宝军1a; 李超1a; 裴斐 1b; 李佳强2; 杨宏秀1a
(1.开滦总医院a.检验科；b.神经外科，河北唐山063000； 2.唐山市截瘫疗养院检验科，河北唐山063003)
Author(s):
WANG Ju-hui1a; YUAN Bao-jun1a; LI Chao 1a; PEI Fei1b; LI Jia-qiang2; YANG Hong-xiu1a
(1 a ．Department of C linical Laboratory；1 b .Department of Neurosurgery，Kailuan General Hospital，Hebei Tangshan 063000，China；2.Department ofClinical Laborat ory，Tangshan Paraplegia Sanatorium，Hebei Tangshan 063003，China)
关键词:
截瘫患者; 细胞免疫功能; T淋巴细胞亚群; B淋巴细胞; NK细胞
分类号:
R64；R392.11
DOI:
10.3969/j.issn.1671-7414.2019.01.010
文献标志码:
A
摘要:
目的测定1976年中国唐山地震生存截瘫患者外周血淋巴细 胞亚群水平，评估截瘫患者40多年后细胞免疫功能的状况。方法采 集110例截瘫患者静脉血，用流式细胞术测定患者T淋巴细胞亚群(包括CD3+，CD4+Th细 胞，CD8+Ts细胞，CD4+/CD8+)，CD19+B淋巴细胞和CD16+CD56+NK细胞，并与正 常对照作比较。同时，按性别分层比较各项目的差异及其之间的相关性。结果 截瘫组与对照组比较，截瘫组CD8+T细胞升高，CD4+/CD8+和CD16+CD56 +NK细胞水平下降，差异均有统计学意义( F=1.375，1.052，0.674，均P <0.05)。 CD3+，CD4+T细胞和CD19+B细胞差异无统计学意义( F=0.800，1.490，0.223，均 P >0.05) 。按性别分层，CD19+B细胞女性组高于男性组，两组结果差异具有统计学意义( F=0.03 8，均P <0.05)，其他指标无明显差异( F=0.002～1.808，均P >0.05)。CD4+与CD 8+，CD4+与CD4+/CD8+，CD4+与CD16+CD56+，CD8+与CD4+/CD8+，CD8 +与CD16+CD56+，CD19+与CD16+CD56+均存在显著相关性( P <0.01)。结论唐山地震截瘫患者细胞免疫功能严重紊乱。
Abstract:
Objective The peripheral blood lymphocyte subsets of p araplegic patients after the 1976 Tangshan earthquake in China were measured and the immune function of paraplegic patients after 40 years was evaluated． Methods Venous blood samples were collected from 110 paraplegi c patients．T lymphocyte subsets (including CD3+，CD4+ Th cells and CD8+Ts cells)，CD19+ B lymphocyte and CD16+ CD56+ NK cells were measured by flow 　cytometry and compared with those of normal controls．The differences among it ems and their correlations were compared by gender stratification．Re sults Compared with the control group，in paraplegic group the level s of CD8+ T cells increased，the levels of CD4+/CD8+ and CD16 +CD56+ N K cells decreased．The difference was statistically significant ( F =1.375，1. 052，0.674，all P <0.05)．There was no significant difference in the number of CD3+，CD4+，CD19+ B cells ( F =0.800，1.490，0.223，all P >0. 05)．The sex stratified CD19+ B cells were higher in the female group than in 　the male group，and the result difference was statistically significant ( F=0 .038，P <0.05)，but there was no significant change in other indexes( F =0 .002～1.808，all P >0.05)．CD4+ and CD8+，CD4+ and CD4+/CD8+，CD 4+ and CD16+CD56+，CD8+ and CD4+/CD8+，CD8+ and CD16+CD56+，CD 19+ and CD16+CD56+ were significantly correlated ( P <0.01)．Conclusion Patients with paraplegia after the Tangshan earthquake have severe cellular immune dysfunction．

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备注/Memo

备注/Memo:

作者简介：王菊惠(1972-)，女，硕士研究生，副主任技师，研究方向：医学检验，流行病与卫生统计学，E-mail：15373588105@163.com。
通讯作者：袁宝军：主任技师，教授，E-mail：ybj25999@163. com。
更新日期/Last Update: 2019-02-28