[1]樊萍a,苟小林b,李娜a.糖耐量受损患者血清eNOS mRNA和TRB3 mRNA表达与疾病干预的相关性研究[J].现代检验医学杂志,2019,34(01):41-46.[doi:10.3969/j.issn.1671-7414.2019.01.011]
　FAN Pinga,GOU Xiao-linb,LI Naa.Correlation Study on Serum Expression of eNOSmRNA and TRB3mRNA，and Disease Intervention in Patients with Impaired Glucose Tolerance[J].Journal of Modern Laboratory Medicine,2019,34(01):41-46.[doi:10.3969/j.issn.1671-7414.2019.01.011]
点击复制

糖耐量受损患者血清eNOS mRNA和TRB3 mRNA表达与疾病干预的相关性研究()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第34卷
期数:
2019年01期
页码:
41-46
栏目:
论著
出版日期:
2019-02-28

文章信息/Info

Title:
Correlation Study on Serum Expression of eNOSmRNA and TRB3mRNA，and Disease Intervention in Patients with Impaired Glucose Tolerance
文章编号:
1671-7414 (2019)01-041-06
作者:
樊萍a; 苟小林b; 李娜a
(咸阳市第一人民医 院a.检验科；b.内分泌科，陕西咸阳712000)
Author(s):
FAN Pinga; GOU Xiao-linb; LI Naa
( a .Departerment of Clinical Laboratory； b .Departerment of Endocrinology，the First People’s Hospital of Xianyang，Shaanxi Xianyang 712000，China)
关键词:
糖耐量受损; 胰岛素抵抗; 生活方式干预治疗; 内皮型一氧化 氮合酶mRNA; 同源蛋白3 mRNA
分类号:
R587；R392.11
DOI:
10.3969/j.issn.1671-7414.2019.01.011
文献标志码:
A
摘要:
目的探讨血清内皮型一氧化氮合酶(eNOS)mRNA和同源蛋白 3(tribbleshomologprotein3，TRB3)mRNA表达在生活方式干预治疗糖耐量受损(IGT)疾病中 的应用价值。方法收集58例接受生活方式干预治疗的糖耐量受损患 者(治疗前组和治疗后组)和53例健康人群(对照组)的血液及临床资料，采用实时荧光定量PC R(real-time-PCR，RT-PCR)技术检测各组研究对象的血清eNOS mRNA和TRB3 mRNA表达。 所有研究对象均接受葡萄糖耐量试验和胰岛素释放试验，通过空腹血糖(FPG)及空腹胰岛素( FINS)水平计算得到胰岛素抵抗指数(HOMA-IR)。采用高效液相色谱仪检测糖化血红蛋白(Hb A1c)水平。比较分析以上指标的变化与IGT的关系。采用受试者工作特征曲线(ROC曲线)评价 血清eNOSmRNA和TRB3mRNA在IGT中的临床意义。结果在对照组、治 疗后组和治疗前组中，血清eNOSmRNA和TRB3mRNA的表达量分别为0.93±0.23，0.69±0. 20，0.19±0.10和0.35±0.14，0.62±0.21，0.96±0.25。TRB3mRNA在治疗前组的 表达显著高于治疗后组，在治疗后组的表达显著高于对照组( F=93.27，P <0.01)；而e NOSmRNA在治疗前组的表达显著低于与治疗后组，在治疗后组的表达显著低于对照组( F=89 .65，P <0.01)，差异均有统计学意义。在对照组、治疗后组和治疗前组中，FPG(mmol/L )，FINs(μU/ml)，HbA1c(%)水平及HOMA-IR分别为5.07±0.41，7.23±7.57，5.29± 0.36，2.13±0.47；5.51±0.47，5.73±0.35，2.87±0.77和6.12±0.59，11 .39±11.71，6.17±0.39，3.17±0.83。治疗前组的FPG ，FINs，HbA1c水平及HOMA-IR分别与治疗后组比较显著增高，治疗后组的各项指标与对照 组比较显著增高，差异均有统计学意义( F=60.03~110.51，均P <0.01)。血清eNOSmRN A 和TRB3mRNA在治疗前组和治疗后组中的表达具有负相关性( r=-0.867，-0.826，均P <0 .01)。血清eNOSmRNA在治疗前组和治疗后组中的表达与FPG，FINs，HbA1c水平及HOMA-IR 呈负相关性( r=-0.779，-0.739，-0.727，-0.755，均P <0.01)，( r=-0.726，- 0.689，-0.667，－0.696，均P <0.01)，而TRB3mRNA在治疗前组和治疗后组中的表达 与FPG，FINs，HbA1c水平及HOMA-IR呈正相关性( r=0.823，0.769，0.753，0.797， 均P <0.01)，( r=0.753，0.697，0.685，0.726，均P <0.01)。ROC曲线分析显示 ，eNOSmRNA和TRB3mRNA的AUC分别为［0.858(95%CI：0.756～0.960， P <0.01)，0. 862(95%CI：0.753～0.970， P <0.01)］。结论血清eNOSmRN A和TRB3mRNA的检测可应用于评价生活方式干预治疗IGT疾病的效果，并可能为IGT及2型糖尿 病的治疗提供有效的基因学依据。
Abstract:
Objective To explore the clinical value of seru m expression of eNOSmRNA and TRB3mRNA in lifestyle intervention treatment for im paired glucose tolerance．Methods These serumal and clini cal data from 58 patients with impaired glucose tolerance，who acquired lifestyl e intervention treatment(pre-treatment group and post-treatment group)，and 53 normal people (control group) were collected．Detecting expression of eNOSmRNA and TRB3mRNA in serum was used the method of the real time fluorescence quantita tive PCR (real-time-PCR，RT-PCR)．All the investigated subjects underwent glu cose tolerance tests and insulin release tests．Insulin resistance index (HOMA- IR) was calculated by fasting blood glucose (FPG) and fasting insulin (FINS) lev els．The level of glycosylated hemoglobin (HbA1c) was detected by high performan ce liquid chromatograph．The relationships between the changes of the above inde xes and IGT were comparatively analyzed．Clinical significances of serum eNOSmRN A and TRB3mRNA in IGT were assessed by ROC curve．Results The expression of serum eNOSmRNA and TRB3mRNA in the control，posttreatment and p retreatment groups were 0.93±0.23，0.69±0.20，0.1 9±0.10 and 0.35±0.14，0.62±0.21，0.96±0.25，respectiyely．The express ion of TRB3mRNA was significantly higher and which of eNOSmRNA was significantly lower in the pretreatment group than those in the post treatment group．Similar situations also occurred in the post treatment group than those in the control group for the two markers，their differences were statistically significant ( F =93.27，89.65， all P <0.01)．The levels of FPG(mmol/L)，FINs(μU/ml)， HbA1c(%) and HOMA-IR in the control group，the treatment group and the pre-tre atment group were (5.07±0.41，7.23±7.57，5.29±0.36，2.13±0.47)，(5. 51±0.47，8.53±7.49，5.73±0.35，2.87±0.77)，(6.12±0.59，11.39±11 .71，6.17±0.39，3.17±0.83)，respectively，which of them in the pre-treat me nt group were significantly higher than those in the post-treatment group，as w ere similar in the post-treatment group compared with those in the control grou p.The above differences were statically significant ( F =60.03~110.51，all P ＜0.001)．It was observed that expression of eNOSmRNA and TRB3mRNA in the pretreatment and posttreatment groups was inversely correlated ( r =-0.867，- 0.826，all P ＜0.01) and eNOSmRNA in the pretreatment and posttreatment gro ups，respectively，help these significant negative correlations with FPG，FINs， HbA1c and HOMA-IR［ r =-0.779，-0.739，-0.727，-0.755， P ＜0.01)，( r =-0.726，-0.689，-0.667，－0.696，all P <0.01) respectively，while TRB3mRNA had these positive correlations［( r =0.823，0.769，0.753，0.797 ，all P ＜0.01)，( r =0.753，0.697，0.685，0.726，all P ＜0.01)］ .The ROC curve analysis showed that the AUC of eNOSmRNA and TRB3mRNA were ［0. 858(95%CI：0.756 to 0.960， P ＜0.01) and 0.862(95%CI：0.753 to 0.970， P ＜0.01)］．Conclusion Detection of serum eNOSmRNA a nd TRB3mRNA can be used evaluating the effect of lifestyle intervention for IGT and may provide an effective genetic basis for treatment of IGT and type 2 diabe tes.

参考文献/References:


[1］龚清海，应焱燕，李辉，等.生活方式干预对成年糖耐量受损患者血糖 影响的系统评价及meta分析［J］.中华内分泌代谢杂志，2014，30(7)：576-581． GONG Qinghai，YIN Yanyan，LI Hui，et al．Relationship between lifestyle interventions and glycemic level in adults with impaired glucose tole rance：asystematic review and meta-analysis［J］．Chinese Journal of End ocrinology and Metabolism，2014，30(7)：576-581．
[2］李金梁，杨兆颖，陈海军，等.瑞舒伐他汀改善胰岛素抵抗的内皮细胞功能的研究 ［J］.中国医刊，2015，50(10)：56-59． LI Jingliang，YANG Zhaoying，CHEN Haijun，et al．Studies on th e improvement of endoth elial cell function in insulin resistance［J］．Chinese Journal of Medicine，201 5，50(10)：56-59．
[3］董少婷．TRIB3基因的研究进展［J］.中国基层医药，2016，23(4)：625-627． DONG Shaoting．Progress of TIRB3 gene research［J］．Chinese Journ al of Primary Medicine and Pharmacy，2016，23(4)：625-627．
[4］孙涛，张熊，张涛．血清chemerinmRNA和TRB3 mRNA检测在2型糖尿病患者胰岛 素抵抗预测中的应用价值［J］.临床和实验医学杂志，2017，16(19)：1922-1925． SUN Tao，ZHANG Xiong，ZHANG Tao．The application of detecting the l evels of serum chemerinmRNA and TRB3mRNA in type 2 diabetes with insulin resista nce［J］．Journal of Clinical and Experimental Medicine，2017，16(19) ：1922-1925．
[5］TABAK A G，JOKELA M，AKBARALY T N，et al．Trajectories of glycaemia ，insulin sensitivity， and insulin secretion before diagnosis of type 2 diabete s：an analysis from the Whitehall II study［J］．Lancet(London England)，2009，3 73(9682)：2215-2221．
[6］LUTZ A H，BLUMENTHAL J B，LANDERS RAM-OS R Q，et al．Exercise-induc ed endothelial progenitor cell mobilization is attenuated in impaired glucose to lerance and type 2 diabetes［J］．J Appl Physiol，2016，121(1)：36-41．
[7］WALDMAN B， JENKINS AJ， DAVIS TM，et al．HDL-C and HDL-C/ApoA-I p redict long-term progression of glycemia in established type 2 diabetes［J］．D iabetes Care，2014，37(8)：2351-2358．
[8］李晓媚，付四海，雷艳萍，等．一氧化氮合酶抑制物在糖尿病大鼠骨骼肌收缩功能 损害中的作用［J］.中国医师杂志，2017，19(10)；1462-1468． LI Xiaomei，FU Sihai，LEI Yanping，et al．Role of nitric oxide synthase inhibitor in the skeletal muscle contractile dysfunction of type 2 di abetic rats［J］．Journal of Chinese Physician，2017，19(10)；1462-1468．
[9］FENG Xiaomeng，XIA Gao，ZHI Yao，et al．Low apo-A-I is associated with insulin resistance in patients with impaired glucose tolerance：a cross-section al study［J］．Lipids Health Disease，2017，16(1)：69．
[10］HASEGAWA Y，SAITO T，OGIHARA T，et al．Blockade of the nu clear factor-κB pathway in the endothelium prevents insulin resistance and pro longs life spans［J］．Circulation，2012，125(9)：1122-1133．
[11］HUANG P L．eNOS，metabolic syndrome and cardiovascular disease［ J］． Trends Endocrinol Metab，2009，20(6)：295-302．
[12］张鹏程，兰崴，刘竹青，等．有氧运动联合抗阻力运动对糖尿病大鼠血管内皮功 能的作用及其对磷脂酰肌醇-3激酶／蛋白激酶B／内皮型一氧化氮合酶通路的影响［J］． 中国糖尿病杂志，2017，25(3)：264-271． ZHANG Pengcheng，LAN Wei，LIU Zhuqing，et al．Effects of aerobic exerc ise combined with resistive exercise on vascular endothelial function and its im pact on Phosphatidylinositol-3 Kinase/Protein Kinase B/Endothelial nitric oxide synthase pathway for diabetic rats［J］．Chinese Journal of Diabetes，2017，25( 3)：264-271．
[13］ONYANGO A N．The contribution of singlet oxygen to insulin r esistance［J］．Oxid Med Cell Longev，2017，7：8765972．
[14］曹梅，赵一卉，韩睿，等．EGCG调控骨骼肌组织TRB3表达改善胰岛素抵抗［J ］．西南师范大学学报(自然科学版)，2017，42(11)： 37-43． CAO Mei，ZHAO Yihui，HAN Rui，et al．EGCG improves insulin resistance by regulating the expression of TRB3 in muscle tissue［J］．Journal of Southwest China Normal University (Natural Science Edition)，2017，42(11) 37-4 3．
[15］董静，刘尊，赵林杰．TRB3在运动改善胰岛素抵抗中的作用［J］.医学综述 ，2012，18(14)：2172-2174． DONG Jing，LIU Zun，ZHAO Linjie．The role of TRB3 in exercise improving insulin resistance［J］．Medical Recapitulate，2012，18(14)：2172-2174．
[16］李琳，徐瑜．生活方式干预对2型糖尿病患者血糖控制的影响［J］.上海交通 大学学报(医学版)，2017，37(6)：871-875． LI Lin，XU Yu．Effects of lifestyle intervention on glucose control in people with type 2 diabetes［J］．Journal of Shanghai Jiaotong Un iversity (Medical Science)，2017，37(6)：871-875．
[17］李全双，邓演超，吴燕，等.初诊2型糖尿病患者综合干预后血栓前状态分子标 志物的变化［J］.现代检验医学杂志，2015，30(2)：36-38． LI Quanshuang，DENG Yanchao，WU Yan，et al．Changes in molecular marke rs of prethrombotic state for newly diagnosed Type 2 diabetic patients after com prehensive intervention［J］．Journal of Modern Laboratory Medicine，2015，30(2) ：36-38．

相似文献/References:

[1]任丽芬,张志平,徐　蓓,等.孕妇葡萄糖耐量和血清胰岛素及 C- 肽释放水平检测对妊娠糖尿病的诊断研究[J].现代检验医学杂志,2020,35(06):167.[doi:doi:10.3969/j.issn.1671-7414.2020.06.041]
　REN Li-fen,ZHANG Zhi-ping,XU Bei,et al.Study on Diagnosis of Gestational Diabetes Mellitus by Detection of GlucoseTolerance, Serum Insulin and C-peptide Release Levels in Pregnant Women[J].Journal of Modern Laboratory Medicine,2020,35(01):167.[doi:doi:10.3969/j.issn.1671-7414.2020.06.041]
[2]丁　洁,何晓一,刘彦君,等.妊娠糖尿病患者胎盘LPL基因Hind片段多态性与新生儿发生胰岛素抵抗风险的相关性研究[J].现代检验医学杂志,2021,36(06):70.[doi:10.3969/j.issn.1671-7414.2021.06.014]
　DING Jie,HE Xiao-yi,LIU Yan-jun,et al.Relevance between Hind Fragment Polymorphism of Placental LPL Gene and the Risk of Neonatal Insulin Resistance in Patients with Gestational Diabetes Mellitus[J].Journal of Modern Laboratory Medicine,2021,36(01):70.[doi:10.3969/j.issn.1671-7414.2021.06.014]
[3]王　伟,张　静,浦　春.妊娠糖尿病患者血清、脐血及胎盘组织中 Visfatin和 Chemerin的表达与胰岛素抵抗发生机制的研究[J].现代检验医学杂志,2022,37(01):21.[doi:10.3969/j.issn.1671-7414.2022.01.005]
　WANG Wei,ZHANG Jing,PU Chun.Expression of Visfatin and Chemerin in Serum, Umbilical Blood and Placenta of Patients with Gestational Diabetes Mellitus andthe Mechanism of Insulin Resistance[J].Journal of Modern Laboratory Medicine,2022,37(01):21.[doi:10.3969/j.issn.1671-7414.2022.01.005]
[4]周 青,秦智娟,马宗丽.妊娠期糖尿病患者血清miR-450a-5p 和CREB 表达水平及临床意义[J].现代检验医学杂志,2022,37(04):102.[doi:10.3969/j.issn.1671-7414.2022.04.020]
　ZHOU Qing,QIN Zhi-juan,MA Zong-li.Expression Levels and Clinical Significance of Serum miR-450a-5p and CREB in Patients with Gestational Diabetes Mellitus[J].Journal of Modern Laboratory Medicine,2022,37(01):102.[doi:10.3969/j.issn.1671-7414.2022.04.020]
[5]李 轶a,尚 立b.非酒精性脂肪肝病患者血清LncRNA PVT1 表达水平与胰岛素抵抗及肝纤维化的相关性研究[J].现代检验医学杂志,2023,38(05):80.[doi:10.3969/j.issn.1671-7414.2023.05.015]
　LI Yia,SHANG Lib.Correlation of Serum LncRNA PVT1 Expression Level with Insulin Resistance and Liver Fibrosis in Patients with Nonalcoholic Fatty Liver Disease[J].Journal of Modern Laboratory Medicine,2023,38(01):80.[doi:10.3969/j.issn.1671-7414.2023.05.015]

备注/Memo

备注/Memo:

作者简介：樊萍(1985-)，女，本科学历，主管检验师，研究方向 ：糖尿病标志物的研究分析，E-mail：1357606542@qq.com。
通讯作者：李娜(1982-)，女，本科学历，主管检验师，研究方 向：糖尿病标志物的研究分析，E-mail：3499337430@qq.com。
更新日期/Last Update: 2019-02-28