[1]李育敏,阚丽娟,张水兰,等.荧光定量PCR测定HBV DNA室间质评结果的回顾分析[J].现代检验医学杂志,2019,34(02):153-155.[doi:10.3969/j.issn.1671-7414.2019.02.039]
　LI Yu-min,KAN Li-juan,ZHANG Shui-lan,et al.Retrospective Analysis on Results in External Quality Assessmentof HBV DNA Determination by Fluorescence Quantitation PCR[J].Journal of Modern Laboratory Medicine,2019,34(02):153-155.[doi:10.3969/j.issn.1671-7414.2019.02.039]
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荧光定量PCR测定HBV DNA室间质评结果的回顾分析()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第34卷
期数:
2019年02期
页码:
153-155
栏目:
质量控制·实验室管理
出版日期:
2019-05-11

文章信息/Info

Title:
Retrospective Analysis on Results in External Quality Assessmentof HBV DNA Determination by Fluorescence Quantitation PCR
文章编号:
1671-7414(2019)02-153-04
作者:
李育敏; 阚丽娟; 张水兰; 汤花梅; 李方勇; 许晓清; 熊 丹; 张秀明
(深圳市罗湖区人民医院医学检验科,广州深圳 518001)
Author(s):
LI Yu-min; KAN Li-juan; ZHANG Shui-lan; TANG Hua-mei; LI Fang-yong; XU Xiao-qing; XIONG Dan; ZHANG Xiu-ming
(Department of Clinical Laboratory,Shenzhen Luohu People's Hospital,Guangdong Shenzhen 518001,China)
关键词:
室间质评;  乙型肝炎病毒核酸;  Z比分数;  标准差指数;  质量控制规则
分类号:
Q503; R466
DOI:
10.3969/j.issn.1671-7414.2019.02.039
文献标志码:
A
摘要:
目的 通过对乙型肝炎病毒核酸(HBV DNA)室间质量评价(external quality assessment,EQA)结果进行回顾分析,探讨检测可能存在的误差,为质量改进提供依据。方法 选取2016～2018年上半年该实验室参加卫计委临检中心HBV DNA项目EQA的结果,采用能力验证(proficiency testing,PT)得分和Z比分数/标准差指数(standard deviation index,SDI)对数据进行统计,绘制休哈特控制图,结合室间质评质量控制规则分析EQA结果。结果 PT得分为100%,结果合格,当前性能为满意,累积性能为成功。│Z│<2.0结果满意,但休哈特图和用SDI和允许误差(allowed error,EA)表示的质控规则分析结果均提示可能存在系统误差。结论 通过Z比分数SDI和选择合适的质控规则对EQA结果进行分析,可以识别HBV DNA定量检测系统存在的潜在系统误差和/或随机误差,以指导实验室进行持续质量改进。
Abstract:
Objective To analyze retrospectively the results of external quality assessment(EQA)of hepatitis B virus(HBV)DNA,and explore the probability of error,and provide the basis for quality improvement.Methods The results of Ministry of Health National Center for Clinical Laboratory external quality assessment for HBV DNA item from 2016 to 2018were collected to analyze retrospectively by proficiency testing(PT)score,Z score or standard deviation index(SDI),Shewhat Chart and quality control rules of EQA.Results The PT score was 100% as pass.The currentperformance was satisfied and the cumulative performance was success.The Zscore was <2.0 as satisfaction.But the Shewhat Chart and combination qualitycontrol rules of SDI and EA demonstrated the probability of systematic error.Conclusion Z score or SDI and quality control rules can be used to analyze EQA data,detect the potential systematic and random errorexisting on HBV DNA quantitative detection system,and guide the improvement ofclinical laboratory quality.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:深圳市医疗卫生三名工程(SZSM201601062)。 作者简介:李育敏(1982-),女,硕士,主治医师,研究方向:分子诊断,E-mail:eliyumin@126.com。。 通信作者:张秀明,E-mail:zxm0760@163.com。 收稿日期:2018-07-06 修回日期:2018-09-26 页码：155+159
更新日期/Last Update: 2019-04-30