[1]刘婷婷,史海峰,刘 静(北部战区总医院检验医学中心,等.急性脑血管病患者ApoE基因多态性临床分布特征及与血脂水平的相关性研究[J].现代检验医学杂志,2019,34(03):25-29.[doi:10.3969/j.issn.1671-7414.2019.03.006]
　LIU Ting-ting,SHI Hai-feng,LIU Jing.Clinical Distribution of ApoE Gene Polymorphism in Patientswith Acute Cerebrovascular Disease and Its Correlation with Blood Lipids[J].Journal of Modern Laboratory Medicine,2019,34(03):25-29.[doi:10.3969/j.issn.1671-7414.2019.03.006]
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急性脑血管病患者ApoE基因多态性临床分布特征及与血脂水平的相关性研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第34卷
期数:
2019年03期
页码:
25-29
栏目:
论著
出版日期:
2019-06-20

文章信息/Info

Title:
Clinical Distribution of ApoE Gene Polymorphism in Patientswith Acute Cerebrovascular Disease and Its Correlation with Blood Lipids
文章编号:
1671-7414(2019)03-025-05
作者:
刘婷婷; 史海峰; 刘 静(北部战区总医院检验医学中心; 沈阳 110016)
(北部战区总医院检验医学中心,沈阳 110016)
Author(s):
LIU Ting-ting; SHI Hai-feng; LIU Jing
(Department of Clinical Laboratory,General Hospital of Northern Theater Command,Shenyang 110016,China)
关键词:
急性脑血管病;  ApoE基因多态性;  血脂
分类号:
R743; R446.11
DOI:
10.3969/j.issn.1671-7414.2019.03.006
文献标志码:
A
摘要:
目的 分析急性脑血管病患者ApoE基因多态性的临床分布特征及与血脂的关系。方法 采用焦磷酸测序技术对311例急性脑血管病患者ApoE基因多态性进行检测。同时检测三酰甘油(TG)、胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)及低密度脂蛋白胆固醇(LDL-C)。结果 共收集2017年3月～2018年2月北部战区总医院住院急性脑血管病患者311例,ApoE各基因型频率为:ε2/ε2型0.03%,ε3/ε3型69.1%,ε4/ε4型1.0%,ε2/ε3型12.9%,ε2/ε4型1.6%和ε3/ε4型15.1%,ApoE表现型分布的频率为:E2占13.2%,E3占70.7%和E4占16.1%。急性脑血管患者ApoE基因型及表现型在男女人群间的分布差异无统计学意义(χ2=0.37～2.31,均P>0.05),在年龄组间的分布差异无统计学意义(χ2=3.30～5.08,均P>0.05),在不同临床诊断中的分布差异也无统计学意义(χ2=0.71～4.54,均P>0.05)。急性脑血管病患者ApoE各基因型在TG,TC,LDL-C和HDL-C浓度水平均差异有统计学意义(F=11.94～19.60,均P<0.05)。急性脑血管病患者ApoE各表现型在TC,HDL-C和LDL-C浓度水平差异有统计学意义(F=6.27～15.59,均P<0.05),但在TG浓度水平差异无统计学意义(F=4.35,均P>0.05)。结论 急性脑血管病患者ApoE基因呈多态性分布,其多态性分布与性别、年龄和临床诊断无关。急性脑血管病患者ApoE基因型和表现型与个体血脂水平存在差异性,临床上可针对急性脑血管病患者的个体化治疗方案,选择合适的降脂药物。
Abstract:
Objective To analyze the clinical distributionof ApoE gene polymorphism in patients with acute cerebrovascular disease and itsrelationship with blood lipids.Methods Pyrosequencing technique was used to detect ApoE gene polymorphism in 311 patients with acute cerebrovascular disease.Meanwhile,triglyceride(TG),cholesterol(TC),high density lipoprotein(HDL-C)and low density lipoprotein(LDL-C)were detected.Results A total of 311 patients with acute cerebrovascular disease were collected from March 2017 to February 2018 in the General Hospital of Northern War Zone.The frequencies of ApoE genotypes were as follows:ε2/ε2 was 0.03%,ε3/ε3 was 69.1%,ε4/ε4 was 1.0%,ε2/ε3 was 12.9%,ε2/ε4 was 1.6% and ε3/ε4 was 15.1%.The frequency of ApoE phenotypes was 13.2% for E2,70.7% for E3 and 16.1% for E4.The distribution of ApoE genotypes and phenotypes in patients with acute cerebrovascular disease had no significant difference between men and women(χ2=0.37～2.31,all P>0.05),the distribution of ApoE genotypes and phenotypes among age groups had no significantdifference(χ2=3.30～5.08,all P>0.05),and the distribution of ApoE genotypes and phenotypes in different clinical diagnosis had no significant difference(χ2=0.71～4.54,all P>0.05).There were significant differences in the levels of TG,TC,LDL-C and HDL-C among the genotypes of ApoE inpatients with acute cerebrovascular disease(F=11.94～19.60,all P<0.05).The ApoE phenotypes in TC,HDL-C and LDL-C of patients with acute cerebrovascular disease had significant difference(F=6.27～15.59,all P<0.05),but there was no significant difference in the levels of TG(F=4.35,P>0.05).Conclusion ApoE gene was polymorphic in patients with acute cerebrovascular disease.The distribution of ApoE gene polymorphism was not related to gender,age and clinical diagnosis.ApoE genotype and phenotypes in patients with acute cerebrovascular disease were correlated with individualblood lipid level.Clinically,individualized treatment programs for patients with acute cerebrovascular disease can be formulated according to different ApoEgenotypes or alleles,and appropriate lipid-lowering drugs can be selected.

参考文献/References:

[1] 刘慧方.ApoE基因多态性与高脂血症患者血脂代谢的关系[J].检验医学,2018,33(11):987-990. LIU Huifang.Relationship between apolipoprotein E gene polymorphismand blood lipid metabolism in hyperlipidemia patients[J].Laboratory Medicine,2018,33(11):897-990.
[2] SOUZA D R,MAKACHIMA L,BIAGIONI R B,et al.Relevance of apolipoproteinE4 for the lipid profile of Brazilian patients with coronary artery disease[J].Braz J Med Biol Res,2007,40(2):189-197.
[3] G(¨overU)Z G,NURHAN OZDEMIR F,SEZER S,et al.Effect of apolipoproteinE polymorphism on serum lipid,lipoproteins,and atherosclerosis in hemodialysispatients[J].American Journal of Kidney Diseases,2000,36(4):826-836.
[4] 章丽萍.ApoE基因在高血压患者后代中的表达情况及其对血脂浓度的影响[J].岭南心血管病杂志,2018,24(6):662-665,696. ZHANG Liping.Expression of apolipoprotein E gene in offspring of patients with hypertension and its effects on blood concentration of lipid[J].South China Journal of Cardiovascular Diseases,2018,24(6):662-665,696.
[5] HORSBURGH K, MCCARRON M O, WHITE F.The role of apolipoprotein E in Alzheimer's disease,acute brain injury and cerebrovascular disease:evidence of common mechanisms and utility of animal models[J].Neurobiology of Aging,2000,21(1):245-255.
[6] ANOOP S,MISRA A,MEENA K,et al.Apolipoprotein E polymorphism in cerebrovascular & coronary heart diseases[J].Indian J Med Res,2010,132(4):363-378.
[7] TAKEDA M,MARTINEZ R,KUDO T,et al.Apolipoprotein E and central nervoussystem disorders: reviews of clinical findings[J].Psychiatry and Clinical Neurosciences,2010,64(6):592-607.
[8] YEN Y C,REBOK G W,GALLO J J,et al.ApoE4 allele is associated with late-life depression:a population-based study[J].Am J Geriatr Psychiatry,2007,15(10):858-868.
[9] RIUS-PéREZ S,TORMOS A M,PéREZ S,et al.Vascular pathology:cause oreffect in Alzheimer disease[J].Neurología(English Edition),2018,33(2):112-120.
[10] KALARIA R N.The role of cerebral ischemia in Alzheimer's disease[J].Neurobiology of Aging,2000,21(2):321-330.
[11] MARTINS I J,HOME E,FOSTER J K,et al.Apolipoprotein E,cholesterol metabolism,diabetes,and the convergence of risk factors for Alzheimer's diseaseand cardiovascular disease[J].Molecular Psychiatry,2006,11(8):721-736.
[12] 刘淑静,常欣魁,陈柯霖,等.载脂蛋白E基因多态性与脑梗患者年龄和血脂的相关性研究[J].标记免疫分析与临床,2018,25(11):1595-1598.LIU Shujing,CHANG Xinkui,CHEN Kelin,et al.A study on the correlation of ApoE gene polymorphism with the age and lipid levels of cerebral infartion[J].Labeled Immunoassays & Clin Med,2018,25(11):1595-1598.
[13] 王京伟,李艳,乔斌,等.华中地区汉族人群SLCO1B1与APOE基因多态性分析及临床意义[J].实用医学杂志,2018,34(18):3041-3046.WANG Jingwei,LI Yan,QIAO Bin,et al.An analysis on the polymorphisms of SLCO1B1 and APOE in central China and its clinical significance[J].The Journal of Practical Medicine,2018,34(18):3041-3046.
[14] 李歆,徐韫健,罗娥.华南地区汉族人群SLCO1B1和ApoE基因多态性分析[J].检验医学与临床,2017,14(6):773-775.LI Xin,XU Yunjian,LUO E.Analysis on genetic polymorphism of SLCO1B1 andApoE among Han population south China area[J].Lab Med Clin,2017,14(6):773-775.
[15] AL HARTHI F,HURAIB G B,ZOUMAN A,et al.Apolipoprotein E gene polymorphism and serum lipid profile in Saudi patients with psoriasis[J].Disease Markers,2014,2014:239645.
[16] 闫恒瑜,谢萍,李燕玲,等.甘肃地区ApoE与SLCO1B1基因多态性及基因型与血脂水平的相关性[J].心脏杂志,2019,30(1):46-49.YAN Hengyu,XIE Ping,LI Yanling,et al.Association of ApoE and SLCO1B1 gene polymorphisms and genotypes with blood lipid levels in Gansu[J].Chin Heart J,2019,30(1):46-49.
[17] 王瑾,陈韬,刘连,等.ApoE和SLCO1B1基因多态性与冠心病的相关性[J].心脏杂志,2018,30(2):150-153.WANG Jin,CHEN Tao,LIU Lian,at al.Correlation of ApoE and SLCO1B1 genepolymorphisms with coronary heart disease[J].Chin Heart J,2018,30(2):150-153.
[18] CORSETTI J P,GANSEVOORT R T,BAKKER S J,et al.Apolipoprotein E predicts incident cardiovascular disease risk in women but not in men with concurrently high levels of high-density lipoprotein cholesterol and C-reactive protein[J].Metabolism,2012,61(7):996-1002.
[19] 孙谦,周辉,郭丽娜.血脂异常人群ApoE和SLCO1B1基因多态性及相关性研究[J].现代检验医学杂志,2017,32(6):28-31.SUN Qian,ZHOU Hui,GUO Lina.Analysis of dyslipidemia correlation and polymorphisms of ApoE and SLCO1B1 gene in dyslipidemia people[J].J Mod Lab Med,2017,32(6):28-31.

备注/Memo

备注/Memo:
作者简介:刘婷婷(1990-),女,硕士研究生,检验医师,主要从事临床检验诊断方面研究,E-mail:ttliu1990@163.com。 通讯作者:刘 静(1983-),女,硕士研究生,主管技师,主要从事临床检验诊断方面研究,E-mail:jingjing8304@163.com。 收稿日期:2019-03-25 修回日期:2019-04-10
更新日期/Last Update: 2019-06-20