[1]刘秋霞,张慧雅.血清MMP-2和MMP-9水平检测作为诊断妊娠期肝内胆汁淤积症新的生物标志物的价值研究[J].现代检验医学杂志,2019,34(03):82-85，90.[doi:10.3969/j.issn.1671-7414.2019.03.020]
　LIU Qiu-xia,ZHANG Hui-ya.Value of Serum MMP-2 and MMP-9 Levels as New Biomarkersfor Diagnosis of Intrahepatic Cholestasis of Pregnancy[J].Journal of Modern Laboratory Medicine,2019,34(03):82-85，90.[doi:10.3969/j.issn.1671-7414.2019.03.020]
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血清MMP-2和MMP-9水平检测作为诊断妊娠期肝内胆汁淤积症新的生物标志物的价值研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第34卷
期数:
2019年03期
页码:
82-85，90
栏目:
论著
出版日期:
2019-06-20

文章信息/Info

Title:
Value of Serum MMP-2 and MMP-9 Levels as New Biomarkersfor Diagnosis of Intrahepatic Cholestasis of Pregnancy
文章编号:
1671-7414(2019)03-082-05
作者:
刘秋霞; 张慧雅
(西电医院妇产科,西安 710077)
Author(s):
LIU Qiu-xia; ZHANG Hui-ya
(Department of Obstetrics and Gynecology,Xidian Hospital,Xi'an710077,China)
关键词:
中国人群;  妊娠期肝内胆汁淤积症;  肝功能;  基质金属蛋白酶;  总胆汁酸
分类号:
R714.255; R392.11
DOI:
10.3969/j.issn.1671-7414.2019.03.020
文献标志码:
A
摘要:
目的 探讨中国人群妊娠期肝内胆汁淤积症(intrahepaticcholestasis of pregnancy,ICP)新的生物标志物,并比较基质金属蛋白酶2(matrix metalloproteinase-2,MMP-2)、基质金属蛋白酶9(matrix metalloproteinase-9,MMP-9)与总胆汁酸(totalbileacids,TBA)水平对ICP的诊断价值。方法 以2012年2月～2016年12月于西电医院妇产科就诊的50例健康妊娠妇女、48例轻度ICP患者和44例重度ICP患者作为研究对象。所有受试者均接受肝功能检查,检查指标包括总胆红素(totalbilirubin,TBIL)、直接胆红素(bilirubin direct,DBIL)、天冬氨酸氨基转移酶(aspartate aminotransferase,AST)、谷氨酸氨基转移酶(alanine aminotransferase,ALT)和甘胆酸(cholyglycine,CG)。检测受试者空腹血清TBA,MMP-2和MMP-9水平,并分析肝功能与这些指标的关系。结果 健康妊娠妇女组、轻度ICP患者组及重度ICP患者组的分娩孕周、新生儿体重差异,均有统计学意义(χ2=8.28～25.08,均P<0.05); 轻度ICP组和重度ICP组诱导分娩比例均明显高于健康的妊娠妇女组,其差异有统计学意义(t=49.66,均P<0.05)。轻度ICP组和重度ICP组TBIL,DBIL,AST,ALT和CG水平均显著高于健康妊娠妇女组,其差异均有统计学意义(t=12.93,14.71,均P<0.05)。健康妊娠妇女组TBA水平低于轻度ICP组和重度ICP组,但差异无统计学意义(t=1.88,1.90,均P>0.05)。轻度ICP组血清MMP-2,MMP-9水平均显著高于健康妊娠妇女组,其差异均有统计学意义(t=10.70,18.66,均P<0.05); 而重度ICP组血清MMP-2,MMP-9水平显著高于轻度ICP组,其差异均有统计学意义(t=8.80,16.64,均P<0.05)。血清MMP-2和MMP-9水平与TBIL,ALT,AST,CG的水平呈持续显著正相关关系(γ>0.5)。结论 血清MMP-2和MMP-9可以作为中国人群ICP诊断和分级的可靠实验室指标。
Abstract:
Objective To investigate novel biomarkers of intrahepatic cholestasis of pregnancy(ICP),and compare the diagnostic significance among matrix metalloproteinase-2(MMP-2),matrix metalloproteinase-9(MMP-9)and totalbileacids(TBA).Methods 50 healthy pregnant women,48 mild ICP patients and 44 severe ICP patients were enrolled from February 2012 to December 2016 for this study.Liver function tests,including serum total bilirubin(TBIL),direct bilirubin(DBIL),aspartate aminotransferase(AST),alanine transaminase(ALT)and cholyglycine(CG),were performed in all participants.After an overnight fast,serum levels of total bile acids(TBA),matrix metalloproteinase 2(MMP-2)and matrix metalloproteinase 9(MMP-9)were measured,and their correlation with liver function tests were analyzed.Results Statistical differences were observed with respect to gestational age at full term,newborn birth weight and induced rate(χ2=8.28～25.08,all P<0.05),not only between healthy pregnant women and mild ICPwomen,but also between mild and severe ICP women.Both mild and severe ICP women exhibited significant elevations(t=49.66,P<0.05)for all tests comparing to healthy pregnant women.TBA concentrations in healthy pregnant women wereprogres sively lower than those of both mild and severe ICP women as well,butthe differences were not statistically significant(t=1.88,1.90,all P>0.05).Serum levels of MMP-2 in mild ICP patients were significantly higherthan that of healthy pregnant women(P<0.05),while MMP-2 serum levels ofsevere ICP patients were also significantly elevated than that of mild ICP patients(P<0.05).Nevertheless,serum levels of MMP-9 also exhibited the sameprogressive trend of elevation as MMP-2 in all three groups of participants.Observed consistent and significant positive correlations between increases in MMP-2 and MMP-9 levels with all liver function test parameters(γ>0.5).Conclusion Serum levels of both MMP-2 and MMP-9 could be reliably used as laboratory abnormalities for accurate diagnosis and sensitive grading of ICP in Chinese population.

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备注/Memo

备注/Memo:
基金项目:陕西省教育厅自然科学研究项目(项目编号:2013JK0773)。 作者简介:刘秋霞(1981-),女,硕士,主治医师,研究方向:妇科肿瘤,E-mail:liuqiuxia1981lqx@163.com。 通讯作者:张慧雅,女,本科,主治医师,研究方向:妇科肿瘤治疗和产后的康复治疗、临床治疗,E-mail:zhanghuiyaxidian@163.com。 收稿日期:2019-02-18 修回日期:2019-04-01
更新日期/Last Update: 2019-06-20