[1]党盼玉,王文婷,刘志新,等.Lewis血型系统抗体检出情况分析及其临床输血策略的探讨[J].现代检验医学杂志,2019,34(03):128-130.[doi:10.3969/j.issn.1671-7414.2019.03.032]
　DANG Pan-yu,WANG Wen-ting,LIU Zhi-xin,et al.Analysis of Lewis Blood Group System Antibody Detectionand Discussion on Clinical Transfusion Strategy[J].Journal of Modern Laboratory Medicine,2019,34(03):128-130.[doi:10.3969/j.issn.1671-7414.2019.03.032]
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Lewis血型系统抗体检出情况分析及其临床输血策略的探讨()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第34卷
期数:
2019年03期
页码:
128-130
栏目:
检验与临床
出版日期:
2019-06-20

文章信息/Info

Title:
Analysis of Lewis Blood Group System Antibody Detectionand Discussion on Clinical Transfusion Strategy
文章编号:
1671-7414(2019)03-128-03
作者:
党盼玉; 王文婷; 刘志新; 尹 文; 胡兴斌
(空军军医大学西京医院输血科,西安 710032)
Author(s):
DANG Pan-yu; WANG Wen-ting; LIU Zhi-xin; YIN Wen; HUXing-bin
(Department of Blood Transfusion,Air Force Medical University Xijing Hospital,Xi'an 710032,China)
关键词:
Lewis血型系统;  血清学试验;  抗Lea抗体;  抗Leb抗体;  输血
分类号:
R457.11
DOI:
10.3969/j.issn.1671-7414.2019.03.032
文献标志码:
A
摘要:
目的 通过对现阶段Lewis血型系统抗体检出率的分析,探讨产生Lewis血型系统抗体患者的临床输血策略。方法 应用盐水试管法及卡式抗人球蛋白法等血清学方法进行实验检测,对证实存在Lewis血型系统抗体的标本进行回顾性分析。结果 2 923例送检标本中有147例检出Lewis抗体,抗Lea抗体83例占56.5%(83/147),抗Leb抗体51例占34.7%(51/147),抗Lea+Leb抗体13例占8.8%(13/147)。单纯IgM型135例占91.8%(135/147),IgG型或IgG+IgM型Lewis抗体12例占8.2%(12/147)。血型正反不一致标本中有38例检出Lewis抗体,其中26例为抗Leb抗体,占68.4%(26/38),卡式抗体筛选试验Lewis抗体漏检率为28.5%(42/147)。51例输注了红细胞,未发生输注无效或输血反应。结论 Lewis血型系统抗体检出率与文献报道[6,9]存在差异,Lewis血型系统抗体的临床意义不可忽视,应尽可能采用三种介质交叉配血来保证输血安全。
Abstract:
Objective To discuss the clinical transfusion strategy on patients producing Lewis blood group system antibody through analysing the antibody detection rate of Lewis blood group system at present.Methods The specificity of irregular antibody was determined by using saltwater medium test tube method,anti-human globulin medium card method and other serological methods.The Lewis blood group system antibody was confirmed the presence in the samples which were analyzed retrospectively.Results Of the 2 923 samples submitted for examination,Lewis antibodywere detected in 147 cases,83 cases of anti-Lea antibody account for 56.5%(83/147),51 cases of anti-Leb antibody account for 34.7%(51/147),and 13cases of anti-Lea+Leb antibody account for 8.8%(13/147).There were 135 cases of pure IgM type accounting for 91.8%(135/147)and 12 cases of IgG type or IgG+IgM type Lewis antibody accounting for 8.2%(12/147).Lewis antibodies were detected in 38 cases of ABO typing discrepancy specimens,among which anti-Leb antibodies were detected in 26 cases,accounting for 68.4%(26/38).Lewisantibody missed rate in the card-type antibody screening test was 28.5%(42/147).There were 51 cases of erythrocyte transfusion,and none of them had ineffective transfusion or transfusion reaction.Conclusion Thedetection of Lewis blood group system antibodies was different from the literature reports[6,9], and the clinical significance of Lewis blood groupsystem antibodies should not be ignored.As far as possible,cross matching of three mediators should be adopted to ensure the safety of blood transfusion.

参考文献/References:

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备注/Memo

备注/Memo:
作者简介:党盼玉(1987-),女,本科,输血技师,主要从事临床输血工作,E-mail:dangpanyu@163.com。 通讯作者:胡兴斌,男,副教授,副主任医师,硕士生导师,主要从事输血医学的基础及临床免疫学研究,E-mail:hxbyqh@fmmu.edu.cn。 收稿日期:2019-03-01 修回日期:2019-03-20
更新日期/Last Update: 2019-06-20