[1]张 丽,王 贺,肖 盟,等.Etest方法与微量肉汤稀释法检测念珠菌属对唑类抗真菌药物的敏感性评价[J].现代检验医学杂志,2019,34(04):67-70.[doi:10.3969/j.issn.1671-7414.2019.04.016]
　ZHANG Li,WANG He,XIAO Meng,et al.Comparison of Etest and Broth Microdilution Method for Antifungal Susceptibility Testing of Candida Species to Azoles[J].Journal of Modern Laboratory Medicine,2019,34(04):67-70.[doi:10.3969/j.issn.1671-7414.2019.04.016]
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Etest方法与微量肉汤稀释法检测念珠菌属对唑类抗真菌药物的敏感性评价()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第34卷
期数:
2019年04期
页码:
67-70
栏目:
论著
出版日期:
2019-08-20

文章信息/Info

Title:
Comparison of Etest and Broth Microdilution Method for Antifungal Susceptibility Testing of Candida Species to Azoles
文章编号:
1671-7414(2019)04-067-04
作者:
张 丽1; 2; 王 贺2; 肖 盟1; 2; 徐英春1; 2
(1.中国医学科学院北京协和医院检验科,北京 100730; 2.侵袭性真菌病机制研究与精准诊断北京市重点实验室,北京 100730)
Author(s):
ZHANG Li1; 2; WANG He2; XIAO Meng1; 2; XUYing-chun1; 2
(1.Department of Clinical Laboratory,Peking Union Medical College Hospital,Chinese Academy of Medical Sciences,Beijing 100730,China; 2.Beijing Key Laboratory forMechanisms Research and Precision Diagnosis of Invasive Fungal Diseases,Beijing 100730,China)
关键词:
念珠菌;  Etest;  微量肉汤稀释法;  唑类药物
分类号:
R379.4; R446.5
DOI:
10.3969/j.issn.1671-7414.2019.04.016
文献标志码:
A
摘要:
目的 比较分析Etest法检测临床常见念珠菌对氟康唑、伏立康唑和伊曲康唑敏感性与微量肉汤稀释法的一致率。方法 针对220株临床常见的5种念珠菌,分别采用CLSI M27微量肉汤稀释法和Etest法进行药物敏感性检测。分别统计Etest法与微量肉汤稀释法检测三种唑类药物的基本一致率(EA)和分类一致率(CA)。结果 Etest方法和微量肉汤稀释法的EA分别为氟康唑(96.8%)、伏立康唑(97.3%)和伊曲康唑(81.8%)。对于氟康唑、伏立康唑和伊曲康唑,白念珠菌、热带念珠菌、近平滑念珠菌、光滑念珠菌和克柔念珠菌中两种检测方法的CA分别为96.2%,97.5%和—; 92.5%,82.5%和97.5%; 92.5%,97.5%和—; 97.5%,—和70%; —,100%和90%。结论 Etest法检测念珠菌对唑类药物敏感性与微量肉汤稀释法一致率较高,是念珠菌唑类药物敏感性测定简便并且准确性较高的检测方法,推荐在真菌实验室常规使用。
Abstract:
Objective To compare the Etest and reference M27-A broth microdilution(BMD)methods for testing susceptibilities of major clinical Candida spp.to fluconazole,voriconazole,and itraconazole.Methods 220 isolates of five Candida species were collected from patients,and were performed susceptibility test according CLSI M27 and Etest.The essential agreement(EA)and categorical agreement(CA)between two methods was evaluated.Results The EA between Etest and BMD forfluconazole,voriconazole,and itraconazole was 96.8%,97.3% and 81.8%,respectively.The CA between Etest and BMD for C.albicans,C.tropicalis,C.parapsilosis,C.glabrata and C.krusei to fluconazole,voriconazole and itraconazole was 96.2%,97.5% and —.92.5%,82.5% and 97.5%.92.5%,97.5% and —.97.5%,— and 70%.—,100% and 90%.respectively.Conclusion This study demonstrated a high agreement between Etest method and brothmicrodilution method,and it is also very easy to perform and read the results.These findings would support the use of the Etest method in a routine mycology service.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金(81572057和81802049),中央高校基本科研业务费(3332019045)。 作者简介:张 丽(1987-),女,博士,主管技师,研究方向:真菌实验室诊断和耐药机制研究,E-mail:zhanglipumchlab@163.com。 通讯作者:徐英春(1964-),男,本科,研究员,研究方向:真菌实验室诊断和耐药机制研究,E-mail:xycpumch@139.com。收稿日期:2019-04-12 修回日期:2019-04-26
更新日期/Last Update: 2019-07-30