[1]申春艳,陆桂琴,于嘉屏.自制超薄型琼脂糖凝胶板电泳分离检测血清LDH同工酶及成年人参考范围的建立[J].现代检验医学杂志,2019,34(04):83-86.[doi:10.3969/j.issn.1671-7414.2019.04.020]
　SHEN Chun-yan,LU Gui-qin,YU Jia-ping.To Separate and Measure the Lactate Dehydrogenase Isoenzymes in Serum by Self-Made Ultra-Thin Agarose Gel Plate Electrophoresis and Establishment of the Reference Intervals in Adult Serum[J].Journal of Modern Laboratory Medicine,2019,34(04):83-86.[doi:10.3969/j.issn.1671-7414.2019.04.020]
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自制超薄型琼脂糖凝胶板电泳分离检测血清LDH同工酶及成年人参考范围的建立()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第34卷
期数:
2019年04期
页码:
83-86
栏目:
论著
出版日期:
2019-08-20

文章信息/Info

Title:
To Separate and Measure the Lactate Dehydrogenase Isoenzymes in Serum by Self-Made Ultra-Thin Agarose Gel Plate Electrophoresis and Establishment of the Reference Intervals in Adult Serum
文章编号:
1671-7414(2019)04-083-04
作者:
申春艳; 陆桂琴; 于嘉屏
(上海迪安医学检验所中心实验室,上海 200433)
Author(s):
SHEN Chun-yan; LU Gui-qin; YU Jia-ping
(Department of Central Laboratory, Shanghai Dian Medical Diagnostic Laboratory,Shanghai 200433,China)
关键词:
琼脂糖凝胶电泳;  乳酸脱氢酶同工酶;  分离检测
分类号:
R446.112
DOI:
10.3969/j.issn.1671-7414.2019.04.020
文献标志码:
A
摘要:
目的 自制超薄型琼脂糖凝胶板,建立电泳分离检测血清乳酸脱氢酶(LDH)同工酶的方法并建立成人血清的参考区间。方法 用自制琼脂糖凝胶板分离检测LDH同工酶,对方法学性能包括精密度、正确度、线性范围、参考区间进行确认和建立。结果 5种LDH同工酶图谱分离清晰,批内CV值均小于8.77%,批间CV值均小于13.12%,胶板间CV值均小于7.89%。LDH1在13.48～287.65U/L,LDH2在18.19～575.67U/L,LDH3在19.42～504.62U/L,LDH4在8.00～342.27U/L和LDH5在13.88～199.79U/L的范围内呈线性,斜率趋近于1。与Sebia配套电泳试剂盒的结果比较,5种同工酶在两方法间的差异无统计学意义(t=0.028 1～0.567 4,均P>0.05),相关系数r=0.949 4～0.985 5。建立的成人参考区间为LDH1:15.7%～34.3%,LDH2:26.8%～38.9%,LDH3:18.8%～28.1%,LDH4:5.7%～13.7%和LDH5:2.7%～15.3%。结论 自制超薄型琼脂糖凝胶板电泳分离检测血清LDH同工酶的方法性能良好,可用于临床检测。
Abstract:
Objective To separate and measure the lactate dehydrogenase(LDH)isoenzymes in serum with self-made ultra-thin agarose gel plate electrophoresis and establish the reference intervals in adult serum.Methods The LDH isoenzymes in serum were measured with the agarose gel electrophoresis.The precision,truness and analytical measurement rangewere validated and the reference intervals in adult serum were established.Results The electrophoresis pattern of 5 LDH isoenzymes wereclear.The within CV values were less than 8.77%,the between CV valueswere less than 13.12%,the between plate CV values were 7.89%.The rangeof LDH1(13.48 U/L～287.65 U/L),LDH2(18.19 U/L～575.67 U/L),LDH3(19.42U/L～504.62 U/L),LDH4(8.00 U/L～342.27 U/L)and LDH5(13.88 U/L～199.79U/L)were linear.The slopes were near 1.Compared with Sebia LDH isoesnzymes electrophoresis kit,the difference of 5 isoenzymes between two methods were not statistically significant(t=0.028 1～0.567 4,all P>0.05)and thesignificant correlation was demonstrated(r=0.949 4～0.985 5).The serum reference ranges of 133 healthy adults were:LDH1(15.7%～34.3%),LDH2(26.8%～38.9%),LDH3(18.8%～28.1%),LDH4(5.7%～13.7%)and LDH5(2.7%～15.3%)respectively.Conclusion The performance of the method to measure LDH isoenzymes with self-made ultra-thin agarose gel plate were favorable.The method is suitable for clinical application.

参考文献/References:

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备注/Memo

备注/Memo:
作者简介:申春艳(1992-),女,技师,主要研究方向为临床生化检验,E-mail:shency@dazd.cn。 通讯作者:于嘉屏(1960-),男,研究员,主要研究方向为临床生化检验,E-mail:yujp@dazd.cn。收稿日期:2019-03-18 修回日期:2019-04-02
更新日期/Last Update: 2019-07-30