[1]肖春海,梁 爽,黄敏洁,等.罗氏U701和希森美康UF-1000i两种尿沉渣分析仪不同分析原理检测细菌结果差异性比较[J].现代检验医学杂志,2019,34(04):132-134.[doi:10.3969/j.issn.1671-7414.2019.04.032]
　XIAO Chun-hai,LIANG Shuang,HUANG Min-jie,et al.Comparison of Bacterial Detection Desults between Roche U701 andSysmex UF-1000i Urine Sediment Analyzer Based on Different Analysis Principles[J].Journal of Modern Laboratory Medicine,2019,34(04):132-134.[doi:10.3969/j.issn.1671-7414.2019.04.032]
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罗氏U701和希森美康UF-1000i两种尿沉渣分析仪不同分析原理检测细菌结果差异性比较()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第34卷
期数:
2019年04期
页码:
132-134
栏目:
研究简报·实验技术
出版日期:
2019-08-20

文章信息/Info

Title:
Comparison of Bacterial Detection Desults between Roche U701 andSysmex UF-1000i Urine Sediment Analyzer Based on Different Analysis Principles
文章编号:
1671-7414(2019)04-132-04
作者:
肖春海; 梁 爽; 黄敏洁; 董志武
(上海市第六人民医院金山分院检验科,上海 201500)
Author(s):
XIAO Chun-hai; LIANG Shuang; HUANG Min-jie; DONG Zhi-wu
(Department of Clinical Laboratory,Jinshan Branch of Shanghai Sixth People's Hospital,Shanghai201500,China)
关键词:
尿有形成分分析;  尿液细菌培养;  尿路感染
分类号:
R446
DOI:
10.3969/j.issn.1671-7414.2019.04.032
文献标志码:
A
摘要:
目的 以中段尿细菌培养为标准,比较荧光染色法和图像智能判读法两种原理的尿沉渣分析仪细菌结果的差异性。方法 对选取上海市第六人民医院金山分院2018年11月疑似尿路感染患者的223份中段尿样品进行细菌培养和鉴定,接种之后,该样品分别在罗氏U701和希森美康UF-1000i上进行检测,最后对尿标本进行离心涂片染色镜检。以培养结果为金标准,分析两台设备的敏感度、特异度和正确度,从而比较两台设备检测细菌的性能。结果 223份尿样品培养出革兰阳性菌20株,革兰阴性菌35株。UF-1000i,离心涂片染色镜检和U701的结果分别与细菌培养结果比较,UF-1000i和离心涂片染色镜检的正确度分别为98.2%和99.1%,且差异无统计学意义(均P>0.05)。U701的三种参考范围的结果中,“-”组、“-～1+”和“-～2+”的正确度分别为67.3%,91.5%和87.4%,与细菌培养结果比较差异均有统计学意义(均P<0.05)。结论 UF-1000i的细菌结果对于尿路感染的提示优于U701,U701细菌结果的参考范围建议为“-～1+”。
Abstract:
Objective The middle-stage urine bacterial culture as the standard, to compare the bacterial results of the two urine sediment analyzers with different principles of fluorescence staining and image intelligent interpretation.Methods 223 mid-stream specimens ofurine in Jinshan Branch of the Shanghai Sixth People's Hospital in November 2018 were cultivated and identified.After the culture,the specimens were testedby Roche U701 and Sysmex UF-1000i respectively.The culture result was as the gold standard.The sensitivity,specificity and coincidence rate of the two devices were analyzed.Then,it was compared that the performance of the bacterial results of the two devices.Results 20 strains of Gram-positive bacteria and 35 strains of Gram-negative bacteria were cultivated in 223urine specimens.The results of UF-1000i,centrifugation staining microscopy and U701 were compared with those of bacterial culture.The coincidence rate of UF-1000i and centrifugal staining microscopy was 98.2% and 99.1%,respectively.And no statistical difference(all P>0.05).The results of the three reference ranges of U701,the coincidence rates of “-” group,“-～1+” and “-～2+” were 67.3%,91.5% and 87.4%,respectively.The results of the three grouping methods of U701 were statistically different from the results of bacterial culture(all P<0.05).Conclusion UF-1000i were betterthan U701 for urinary tract infection in the bacterial results.The reference range for U701 bacterial result was recommended as “-～1+”.

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备注/Memo

备注/Memo:
基金项目:上海市第六人民医院联合体课题(编号:2017-01)。 作者简介:肖春海(1976-),男,硕士,副主任技师,主要从事临床检验与实验室管理,E-mail; xchwjf@163.com。收稿日期:2019-04-04 修回日期:2019-04-29
更新日期/Last Update: 2019-07-30