[1]刘 炳a,郭 欣b,郑 凡a,等.四川省宜宾地区健康成人外周血淋巴细胞亚群参考区间的建立[J].现代检验医学杂志,2019,34(05):101-105.[doi:10.3969/j.issn.1671-7414.2019.05.025]
　LIU Binga,GUO Xinb,ZHENG Fana,et al.Establishment of Reference Intervals for Healthy Adult PeripheralBlood of Lymphocyte Subsets in Yibin City of Sichuan Province[J].Journal of Modern Laboratory Medicine,2019,34(05):101-105.[doi:10.3969/j.issn.1671-7414.2019.05.025]
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四川省宜宾地区健康成人外周血淋巴细胞亚群参考区间的建立()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第34卷
期数:
2019年05期
页码:
101-105
栏目:
论著
出版日期:
2019-10-29

文章信息/Info

Title:
Establishment of Reference Intervals for Healthy Adult PeripheralBlood of Lymphocyte Subsets in Yibin City of Sichuan Province
文章编号:
1671-7414(2019)05-101-05
作者:
刘 炳a; 郭 欣b; 郑 凡a; 江燕红a; 蒋淼璐a
(宜宾市第二人民医院a.检验科; b.普外科,四川宜宾 644000)
Author(s):
LIU Binga; GUO Xinb; ZHENG Fana; JIANG Yan-honga; JIANG Miao-lua (a.
Deparment of Clinical Laboratory; b.Deparment of General Surgery,the First People's Hospital of Yibin,Sichuan Yibin 644000,China)
关键词:
健康成人;  流式细胞术;  淋巴细胞亚群;  参考区间
分类号:
R446.62
DOI:
10.3969/j.issn.1671-7414.2019.05.025
文献标志码:
A
摘要:
目的 建立宜宾地区健康成人外周血淋巴细胞亚群参考区间。方法 收集303例健康成人外周血标本(年龄18～86岁,其中男性171例,女性131例),并按性别、年龄分组,采集空腹静脉抗凝全血后4 h内完成检测。根据美国临床和实验室标准协会(clinical laboratory standards institute,CLSI)对检验项目的参考区间设定的推荐程序,以正态分布法建立年龄或性别分组的淋巴细胞亚群参考区间。结果 CD3+青、中年组男性,老年组男性,青、中年组女性和老年组女性的参考区间分别为53.7%～84.7%,45.83%～83.03%,55.45%～85.85%和48.01%～87.29%,分别与原来使用参考区间进行单样本均数t检验,t=6.266,-3.522,9.647,2.259,均P<0.05; CD3+(个/μl)青、中年组,老年组参考区间分别为482.37～2 113.53,307.35～1 741.21,与原来使用参考区间进行单样本均数t检验,t=-20.859,-36.755,均P<0.05; CD3+CD8+青年组,中、老年组参考区间分别为16.59%～43.59%,11.19%～42.23%,与原来使用参考区间进行单样本均数t检验,t=12.483,3.429,均P<0.05; CD3+CD8+(个/μl)青、中年组,老年组参考区间分别为73.94～1000.08,26.26～818.38,与原来使用参考区间进行单样本均数t检验,t=-13.373,-25.518,均P<0.05; CD3+CD4+青年组,中、老年组参考区间分别为20.99%～47.61%,21.91%～53.07%,与原来使用参考区间进行单样本均数t检验,t=-28.718,-17.541,均P<0.05; CD3+CD4+(个/μl)青、中、老年组参考区间为391.37～1 284.69,与原来使用参考区间进行单样本均数t检验,t=-35.178,P<0.05; CD4+/CD8+青年组,中、老年组参考区间分别为0.43～2.03,0.26～2.84,与原来使用参考区间进行单样本均数t检验,t=-22.718,-5.671,均P<0.05); CD3-CD16+CD56+青、中年组男性,老年组男性,青、中年组女性,老年组女性参考区间分别为3%～34.56%,3.93%～43.63%,2.88%～31.10%和0.74%～38.52%,与原来使用参考区间进行单样本均数t检验,t=8.842,15.605,5.537,8.904,均P<0.05; CD3-CD16+CD56+(个/μl)青、中、老年组男性,青、中、老年组女性参考区间分别为47.51～713.67,39.49～586.95,与原来使用参考区间进行单样本均数t检验,t=1.341,-6.767,P值分别为P>0.05,P<0.05; CD3-CD19+青、中、老年组参考区间为3.32%～18.02%,与原来使用参考区间进行单样本均数t检验,t=-28.227,P<0.05; CD3-CD19+(个/μl)青、中、老年组参考区间为42.99～353.45,与原来使用参考区间进行单样本均数t检验,t=-44.576,P<0.05。除CD3-CD16+CD56+(个/μl)青、中、老年组男性外,说明该实验建立的参考区间与原参考区间差异均具有统计学意义。结论 初步建立了宜宾地区健康成人外周血淋巴细胞亚群参考区间,为今后宜宾地区艾滋病及相关疾病的诊断、治疗以及疗效评价提供依据。
Abstract:
Objective To establishment of reference intervals for peripheral blood lymphocyte subsets in healthy adult in Yibin.Methods 303 healthy adult indivicuals(172 men and 131 women)agedfrom 1 years to 86 years were enrolled.Venous anticoagulation was detected within 4 hours.Recommended procedures based on CLSI,sreference for inspection items,and normal distribution method was used to establishment of reference Interval of lymphocyte subsets in age or sex groups.Results Thereference intervals of CD3+(%)in young and middle-aged males,old age group males,young and middle-aged females,old age group females were 53.7%～84.7,45.83%～83.03%,55.45%～85.85% and 48.01%～87.29% respectively,and single sample mean t test with original reference interval(t=6.266,-3.522,9.647,2.259,all P<0.05).The reference intervals of CD3+(piece/μl)in young and middle-aged group,old age group were 482.37～2 113.53(piece/μl)and 307.35～141.21(piece/μl)respectively,and single sample mean t test with original reference interval(t=-20.859,-36.755,all P<0.05).The reference intervals of CD3+ CD8+(%)in young age group,middle aged and old group were 16.59%～43.59% and 11.19%～42.23% respectively,and single sample mean t test with original reference interval(t=12.483,3.429,P<0.05).The reference intervals of CD3+ CD8+(piece/μl)in young andmiddle-aged,old age group were 73.941～000.08(piece/μl),26.26～818.38(piece/μl),respectively,and single sample mean t test with original reference interval(t=-13.373,-25.518,P<0.05).The reference intervals of CD3+ CD4+(%)in young age group,middle aged and old group were 20.99%～47.61%,21.91%～53.07% respectively,and single sample mean t test with originalreference interval(t=-28.718,-17.541,P<0.05).The reference intervalsof CD3+CD4+(piece/μl)in all year group was 391.371～1 284.69(piece/μl)respectively,and single sample mean t test with original reference interval(t=-35.178,P<0.05).The reference intervals of CD4+/CD8+ in young age group,middle aged and old group were 0.43～2.03 and 0.26～2.84 respectively,and single sample mean t test with original reference interval(t=-22.718,-5.671,P<0.05).The reference intervals of CD3-CD16+ CD56+(%)inyoung and middle-aged males,old age group males,young and middle-aged females,old age group females were 3%～34.56%,3.93%～43.63%,2.88%～31.10% and 0.74%～38.52% respectively,and single sample mean t test with original reference interval(t=8.842,15.605,5.537,8.904,all P<0.05).The reference intervals of CD3-CD16+ CD56+(piece/μl)in all year group males,all year group females were 47.51～713.67(piece/μl)and 39.49～586.95(piece/μl),respectively,and single sample mean t test with original reference interval(t=1.341,P>0.05 and t=-6.767,P<0.05).The reference interval for CD3-CD19+(%)in all year group was 3.32%～18.02%,and single sample mean t test with original reference interval(t=-28.227,P<0.05).The reference interval for CD3-CD19+(piece/μl)in all year groupwas 42.99～353.45(piece/μl),and single sample mean t test with originalreference interval(t=-44.576,P<0.05).Except for all year group males in CD3-CD16+ CD56+(piece/μl),indicating that the difference between thereference interval and the original reference interval established in this experiment was statistically significant.Conclusion The reference interval of peripheral blood lymphocyte subsets of healthy adults in Yibinarea was preliminarily established,which provided a basis for the diagnosis,treatment and evaluation of AIDS and related diseases in Yibin.

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备注/Memo

备注/Memo:
作者简介:刘 炳(1983-),男,大学本科,主管技师,研究方向:主要从事临床血液学检验及流式细胞学检验,E-mail:317158741@qq.com。
更新日期/Last Update: 2019-10-20