[1]赵 娜a,康利宝b.创伤性膝骨关节炎患者血清IL-6,TNF-α,瘦素水平表达与影像学K-L分级的相关性[J].现代检验医学杂志,2019,34(05):139-142.[doi:10.3969/j.issn.1671-7414.2019.05.035]
　ZHAO Naa,KANG Li-baob.Association between Levels of Serum Inflammatory Cytokines and Imaging Severity in Patients with Post-traumatic Knee Osteoarthritis[J].Journal of Modern Laboratory Medicine,2019,34(05):139-142.[doi:10.3969/j.issn.1671-7414.2019.05.035]
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创伤性膝骨关节炎患者血清IL-6,TNF-α,瘦素水平表达与影像学K-L分级的相关性()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第34卷
期数:
2019年05期
页码:
139-142
栏目:
检验与临床
出版日期:
2019-10-29

文章信息/Info

Title:
Association between Levels of Serum Inflammatory Cytokines and Imaging Severity in Patients with Post-traumatic Knee Osteoarthritis
文章编号:
1671-7414(2019)05-139-04
作者:
赵 娜a; 康利宝b
(榆林市第二医院a.检验科; b.输血科,陕西榆林 719000)
Author(s):
ZHAO Naa; KANG Li-baob
(a.Department of Clinical Laboratory; b.Department of Blood Transfusion,the Second Hospital of Yulin,Shaanxi Yulin 719000,China)
关键词:
创伤性膝骨关节炎;  促炎症因子;  Kellgren-Lawrence分级
分类号:
R684.3; R392.11
DOI:
10.3969/j.issn.1671-7414.2019.05.035
文献标志码:
A
摘要:
目的 探讨创伤性膝骨关节炎(post-traumatic knee osteoarthritis,PTOA)患者血清促炎症因子的表达及其与影像学分级严重程度的相关性。方法 收集2013年1月～2017年12月在陕西省榆林市第二医院确诊的63例PTOA患者,30例健康体检者的临床资料纳入研究。采用酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)法检测受试者血清中促炎症因子(IL-6,TNF-α和瘦素)的水平。结果 与健康对照组相比,PTOA患者血清中促炎症因子(IL-6,TNF-α和瘦素)水平显著增高(IL-6:15.87±5.59 ng/L vs 7.04±2.42 ng/L; TNF-α:41.40±14.63 ng/L vs 24.53±10.37 ng/L; 瘦素:15.52±6.43 μg/L vs 5.21±3.19 μg/L),差异均有统计学意义(t=7.961,5.164,8.292,均P<0.001)。进一步通过K-L分级对影像学严重程度分为4组,结果显示4组间血清促炎症因子水平差异有统计学意义(F=5.974,7.090,均P<0.001),且血清促炎症因子(IL-6,TNF-α和瘦素)水平随着膝关节损伤严重程度增加而增高。相关性分析显示血清IL-6与TNF-α(r=0.458)、瘦素(r=0.563)呈正相关,TNF-α与瘦素(r=0.692)呈正相关(均P<0.001)。结论 血清促炎症因子(IL-6,TNF-α和瘦素)水平在PTOA患者显著增高,且与K-L分级密切相关,可作为一个潜在的生物标记物反映PTOA患者膝关节损伤的严重程度。
Abstract:
Objective To investigate the levels of serum inflammatory cytokines in patients with post-traumatic knee osteoarthritis(PTOA)and the correlation with the severity of imaging.Methods 63 cases of PTOA patients and 30 cases of healthy control,admitted to the Second Hospital of Yulin from January 2013 to December 2017,were enrolled in thisstudy.The levels of serum inflammatory cytokines(IL-6,TNF-α and leptin)was detected by enzyme linked immunosorbent assay(ELISA).Results The levels of serum inflammatory cytokines(IL-6,TNF-α and leptin)were significantly increased in PTOA patients compared with healthy controls(HC)(IL-6:15.87±5.59 ng/L vs 7.04±2.42 ng/L,TNF-α:41.40±14.63 ng/L vs24.53±10.37 ng/L,leptin:15.52±6.43 μg/L vs 5.21±3.19 μg/L),the differences were statistically significant(t=7.961,5.164 and 8.292,allP<0.001).The severity of imaging was further divided into 4 groups by Kellgren-Lawrence grade,the results showed that the levels of serum proinflammatory cytokines(IL-6,TNF-α and leptin)were significantly different among the 4groups(F=5.974,7.090,all P<0.001),and the level of serum proinflammatory cytokines(IL-6,TNF-α and leptin)were increases with the severityof knee injury.Correlation analysis showed that serum IL-6 was positively correlated with TNF-α(r=0.458)and leptin(r=0.563),and TNF-α was positively correlated with leptin(r=0.692)(all P<0.001).Conclusion The levels of serum proinflammatory cytokines(IL-6,TNF-α and leptin)were increased in patients with PTOA and was associated with K-Lgrade and suggesting that proinflammatory cytokines may be a potential markersfor reflect the severity of knee joint injury in patients with PTOA.

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备注/Memo

备注/Memo:
基金项目:陕西省自然科学基金项目(2013JM4016)。 作者简介:赵 娜(1983-),女,本科,主管检验师,研究方向:临床医学检验,E-mail:yuzhaon@163.com。 通讯作者:康利宝(1979-),男,本科,主管检验师,E-mail:1144814852@qq.com。
更新日期/Last Update: 2019-10-20