[1]王　蓉,陈紫茹,唐露丹,等.广州市第一人民医院新生儿病区金黄色葡萄球菌毒力基因分布分析[J].现代检验医学杂志,2019,34(06):63-66.[doi:10.3969 ／ j.issn.1671-7414.2019.06.015]
　WANG Rong,CHEN Zi-ru,TANG Lu-dan,et al.Analysis of Virulence Gene Distribution of Staphylococcus Aureus in Neonatal Disease Area from the First People’s Hospital of Guangzhou[J].Journal of Modern Laboratory Medicine,2019,34(06):63-66.[doi:10.3969 ／ j.issn.1671-7414.2019.06.015]
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广州市第一人民医院新生儿病区金黄色葡萄球菌毒力基因分布分析 ()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第34卷
期数:
2019年06期
页码:
63-66
栏目:
论著
出版日期:
2019-12-30

文章信息/Info

Title:
Analysis of Virulence Gene Distribution of Staphylococcus Aureus in Neonatal Disease Area from the First People’s Hospital of Guangzhou
文章编号:
1671-7414（2019）06-063-04
作者:
王　蓉; 陈紫茹; 唐露丹; 杨　潇
（广州市第一人民医院检验科，广州　510180）
Author(s):
WANG Rong;  CHEN Zi-ru;  TANG Lu-dan;  YANG Xiao
（Department of Clinical Laboratory, the First People’s Hospital of Guangzhou, Guangzhou　510180， China）
关键词:
新生儿; 金黄色葡萄球菌; 毒力基因
分类号:
R378.11；R446.5
DOI:
10.3969 ／ j.issn.1671-7414.2019.06.015
文献标志码:
A
摘要:
目的　了解广州市第一人民医院新生儿病区的金黄色葡萄球菌毒力基因的分布情况，揭示其分子流行特征， 为进一步建立有效防控措施奠定基础。方法　选取2018 年6 月~2019 年4 月新生儿病区分离出金黄色葡萄球菌22 株， PCR 方法检测金黄色葡萄球菌纤维蛋白原结合蛋白（clfa）、胶原蛋白结合蛋白（cna）、纤连蛋白连接蛋白A（fnbpA）、 溶血素A，B（hlaA, hlaB）基因、金黄色葡萄球菌A 蛋白（spa）基因、mecA 基因、表皮剥脱素（eta）基因和中毒性 休克综合征毒素（tsst-1）基因。使用四格表资料和R×C 表的χ2 检验进行统计学分析。结果　22 株金黄色葡萄球菌中， 有6 株未检测到所选择的9 个基因。9 个毒力基因中hlaB，eta 和tsst-1 基因检出率低于10% （分别为9.09%，9.09% 和4.55%），随后依次为fnbpA 基因（13.64%），mecA 基因（27.27%）和cna 基因（45.45%）。clfa，hlaA 和spa 基 因检出率最高（分别为68.18%，63.64% 和59.09%）不同基因检出率差异具有统计学意义（χ2=240.21，P =0.000）。 clfa+hlaA+spa+cna 毒力基因模式检出率最高，占25%，差异具有统计学意义（χ2=69.607，P =0.000）。结论　广州市第 一人民医院新生儿病区金黄色葡萄球菌大都携带多个毒力基因，毒力基因检出率较低。
Abstract:
Objective　To understand the distribution of virulence genes of Staphylococcus aureus in neonatal areas of the First People’s Hospital of Guangzhou, reveal the molecular epidemic characteristics, and lay a foundation for the further establishment of effective prevention and control measures. Methods　From June 2018 to April 2019, 22 strains of Staphylococcus aureus were isolated from neonatal disease area. The adhesion toxin gene (clfa,cna,fnbpA) and hemolysin gene (hlaA,hlaB), spa gene, mecA gene, epiderin gene (eta), and toxic shock syndrome toxin gene (tsst-1), a total of 9 genes of Staphylococcus aureus were detected by PCR. Four-lattice and the R×C table’s χ2 tests were adopted to statistical analysis. Results　Out of 22 strains staphylococucs aureus, 9 genes were not detected in 6 of the 22 strains.Among the 22 strains, the positive rates of hlaB, eta and tsst-1 gene were below 10% (9.09%, 9.09% and 4.55%, respectively), followed by cna gene (45.45%), mecA gene (27.27%), fnbpA gene (13.64%). The positive rates of clfa, hlaA and spa genes were about 60% (68.18%, 63.64% and 59.09% respectively), the detection rates of different genes were statistically significant (χ2=240.21，P =0.000). The highest detection rate of virulence gene mode was clfa+hlaA+spa+cna, accounting for 25%, the different was statistically significant (χ2=49.607，P =0.000). Conclusion　Most of the strains of Staphylococcus aureus in neonatal areas carried many virulence genes, but the positive rate of virulence genes was lower than that other researches.

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备注/Memo

备注/Memo:
基金项目：广东省自然基金（2014A030313677）；广东省中医药局建设中医药强省科研课题（20111006）；广东省科技计划项目 （2012B040304015）；广东省自然科学基金（2018A0303130168）；广州市卫生健康科技项目（20191A011013）。 作者简介：王蓉（1970 －），女，大专，主管技师，研究方向：感染性疾病的致病机理，E-mail：banglaoxu@163.com。 通讯作者：杨潇（1985 －），男，博士研究生，主管技师，研究方向：感染性疾病的致病机理，E-m
更新日期/Last Update: 2019-12-25