[1]李杰萍a,邹建平,江絮萍b,等.Omega-3 多不饱和脂肪酸衍生物18-HEPE 对人子宫内膜癌细胞株侵袭的抑制作用研究[J].现代检验医学杂志,2020,35(01):16-19.[doi:10.3969/j.issn.1671-7414.2020.01.005]
　LI Jie-pinga,ZOU Jian-ping,JIANG Xu-pingb,et al.Suppression of Omega-3 PUFAs Derivative 18-HEPE on the Invasion of Endometrial Cancer Cell Lines[J].Journal of Modern Laboratory Medicine,2020,35(01):16-19.[doi:10.3969/j.issn.1671-7414.2020.01.005]
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Omega-3 多不饱和脂肪酸衍生物18-HEPE 对人子宫内膜癌细胞株侵袭的抑制作用研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第35卷
期数:
2020年01期
页码:
16-19
栏目:
论　　著
出版日期:
2020-02-29

文章信息/Info

Title:
Suppression of Omega-3 PUFAs Derivative 18-HEPE on the Invasion of Endometrial Cancer Cell Lines
文章编号:
1671-7414(2020)01-016-04
作者:
李杰萍1a; 邹建平2; 江絮萍1b; 杨 琳2; 刘桐宇2; 谢 榕2
(1.武警福建总队医院 a.检验科; b.妇产科, 福州 350003; 2.福建省肿瘤医院妇科肿瘤外科,福州 350014)
Author(s):
LI Jie-ping1a;  ZOU Jian-ping2;  JIANG Xu-ping1b;  YANG Lin2;  LIU Tong-yu2;  XIE Rong2
(1a. Department of Clinical Laboratory; 1b. Department of Obstetrics and Gynecology,General Hospital of FujianCorps of CAPF, Fuzhou 350003,China; 2. Department of GynecologicOncology,Fujian Provincial Cancer Hospital, Fuzhou 350014,China)
关键词:
Omega-3多不饱和脂肪酸;  子宫内膜癌;  细胞侵袭
分类号:
R737.33; R730.43
DOI:
10.3969/j.issn.1671-7414.2020.01.005
文献标志码:
A
摘要:
目的 探讨Omega-3多不饱和脂肪酸衍生物18-HEPE对子宫内膜癌细胞株侵袭的影响。方法 采用Transwell小室侵袭实验观察18-HEPE对子宫内膜癌细胞HEC-1B和RL 95-2侵袭的影响。用Western blot和实时定量PCR分析18-HEPE处理后子宫内膜癌细胞侵袭相关基因MMP-2,MMP-9以及炎症因子IL-6的表达变化。进一步采用Western blot观察18-HEPE对子宫内膜癌细胞PTEN表达的影响。结果 18-HEPE能剂量依赖性地抑制子宫内膜癌细胞HEC-1B和RL 95-2的侵袭,100 pg/ml 18-HEPE能明显抑制子宫内膜癌细胞MMP-2,MMP-9以及IL-6的表达。同时,100 pg/ml 18-HEPE能显著升高子宫内膜癌细胞PTEN的表达。结论 18-HEPE可以通过多种环节抑制子宫内膜癌细胞的侵袭,有广阔的临床应用前景。

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备注/Memo

备注/Memo:
基金项目:福建省自然科学基金项目(2016J01405)。作者简介:李杰萍(1975-),男,博士,副主任技师,从事脂类代谢与肿瘤研究,E-mail:liejieping@hotmail.com。邹建平,共同第一作者。收稿日期:2019-10-21 修回日期:2019-11-31
更新日期/Last Update: 2020-03-30