[1]谢国艳,蔡 枫,梁 斌,等.mCIM 法与PAE-MHT 法检测铜绿假单胞菌产金属β- 内酰胺酶的性能评价[J].现代检验医学杂志,2020,35(01):57-59.[doi:10.3969/j.issn.1671-7414.2020.01.015]
　XIE Guo-yan,CAI Feng,LIANG Bing,et al.Evaluation of Modified Carbapenem Inactivation Method and PseudomonasAeruginosa-MoeifiedHodge Test for Detection of Metallo-Lactamase- Producing Pseudomonas Aeruginosa[J].Journal of Modern Laboratory Medicine,2020,35(01):57-59.[doi:10.3969/j.issn.1671-7414.2020.01.015]
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mCIM 法与PAE-MHT 法检测铜绿假单胞菌产金属β- 内酰胺酶的性能评价()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第35卷
期数:
2020年01期
页码:
57-59
栏目:
论　　著
出版日期:
2020-02-29

文章信息/Info

Title:
Evaluation of Modified Carbapenem Inactivation Method and Pseudomonas Aeruginosa-MoeifiedHodge Test for Detection of Metallo-Lactamase- Producing Pseudomonas Aeruginosa
文章编号:
1671-7414(2020)01-057-04
作者:
谢国艳; 蔡 枫; 梁 斌; 裴凌燕; 冯 琪
(上海中医药大学附属市中医医院检验科,上海 200071)
Author(s):
XIE Guo-yan;  CAI Feng;  LIANG Bing;  PEI Ling-yan;  FENG Qi
(Department of Clinical Laboratory, Shanghai Hospital of Traditional Chinese Medicine Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200071, China)
关键词:
改良碳青霉烯类失活法;  铜绿假单胞菌;  金属β-内酰胺酶;  评估
分类号:
R378.991; R446.5
DOI:
10.3969/j.issn.1671-7414.2020.01.015
文献标志码:
A
摘要:
目的 评估改良的碳青霉烯类灭活法(mCIM)和铜绿假单胞菌改良Hodge试验(PAE-MHT)法对铜绿假单胞菌(PAE)产金属β-内酰胺酶(MBLs)的表型筛选能力。 方法 收集176株耐碳青霉烯类PAE,PCR检测IMP 和VIM基因,采用mCIM法和PAE-MHT法检测产MBLs菌株,其中mCIM法分别选择亚胺培南(IMP)和美罗培南(MEM)作为药物底物进行筛选能力的研究,对几种方法与PCR检测结果的一致性进行统计分析。结果 以IMP为底物mCIM法和以MEM为底物mCIM法检测MBLs的阳性率差异无统计学意义(χ2=0.01,P>0.05)。以IMP为底物mCIM法、以MEM为底物mCIM法和PAE-MHT法的准确度分别为94.9%,96.6%和94.3%; 敏感度分别为90.5%,100%和85.7%; 特异度分别为95.5%,96.1%和95.5%。三者在准确度、敏感度和特异度之间两两相比,差异均无统计学意义(χ2=0.01～2.99,均P>0.05)。三种方法与PCR法均具有良好的一致性。结论 PAE-MHT法经济、操作简便,结果易于判读。以MEM为底物的mCIM法具有高敏感度与高特异度,更适合于检测PAE的MBLs。
Abstract:
Objective To evaluate the screening capacity of modified carbapenem inactivation method(mCIM)and Pseudomonas aeruginosa-Moeified Hodge(PAE-MHT)test for detection of metallo-β-lactamase-producing(MBLs)in PAE.Methods 176 carbapenem-resistant PAE clinical isolates were dectected to MBLs by mCIM and PAE-MHT test, respectively. Meanwhile, IMP genes and VIM genes were detected by PCR. The examination results were analyzed statistically. Results Comparing mCIM of imipenem-substrate methods to mCIM of meropenem-substrate method, the dectction rates of MBLs were not statistically significant(χ2=0.01,P>0.05). For mCIM of imipenem-substrate, mCIM of meropenem-substrate and PAE-MHT, the accuracy rate was 94.9%, 96.6% and 94.3%, respectively. The sensitivity was 90.5%, 100% and 85.7%, respectively. The specificity was 95.5%, 96.1% and 95.5%,respectively. Pairwise comparison of accuracy, sensitivity and specificity among the three groups showed no statistically significant difference(χ2=0.001～2.99, all P>0.05). Three methods had a good consistency compared with PCR.Conclusion PAE-MHT test is a method of economical, practical and simple operation. The mCIM of meropenem-substrate is high sensitivity and good specificity for detecting MBLs-producing PAE.

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备注/Memo

备注/Memo:
作者简介:谢国艳(1974-)女,硕士,副主任医师,主要从事临床微生物学检测及耐药研究,E-mail:xieguoyan93@163.com。 通讯作者:蔡枫,主任技师,硕导,主要从事检验医学、临床免疫学方面的研究,E-mail:2984821930@qq.com。收稿日期:2019-08-05 修稿日期:2019-08-23
更新日期/Last Update: 2020-03-30