[1]罗文强,茹 平.血清Gas6 和TSP-1 水平与糖尿病视网膜病变的相关性研究[J].现代检验医学杂志,2020,35(01):71-74.[doi:10.3969/j.issn.1671-7414.2020.01.019]
　LUO Wen-qiang,RU Ping.Correlational Research on the Levels of Gas6and TSP-1 in Serumin Diabetic Retinopathy[J].Journal of Modern Laboratory Medicine,2020,35(01):71-74.[doi:10.3969/j.issn.1671-7414.2020.01.019]
点击复制

血清Gas6 和TSP-1 水平与糖尿病视网膜病变的相关性研究 ()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第35卷
期数:
2020年01期
页码:
71-74
栏目:
论　　著
出版日期:
2020-02-29

文章信息/Info

Title:
Correlational Research on the Levels of Gas6and TSP-1 in Serumin Diabetic Retinopathy
文章编号:
1671-7414(2020)01-071-04
作者:
罗文强1; 茹 平2
(1.陕西省咸阳市中心医院检验科,陕西咸阳 712000.2.西安市第八医院检验科,西安 710061)
Author(s):
LUO Wen-qiang1;  RU Ping2
(1. Department of Clinical Laboratory,the Central Hospital of Xianyang,Shaanxi Xianyang 712000, China; 2. Department of Clinical Laboratory,the Eighth Hospital of Xi'an, Xi'an 710061,China)
关键词:
2型糖尿病;  糖尿病视网膜病变;  血清生长停滞特异基因6(Gas6);  血小板反应蛋白-1(TSP-1);  空腹血糖(FSG);  糖化血红蛋白A1c(HbA1c);  视网膜中央动脉;  中央动脉收缩期最大流速(PSV);  舒张末期血流速度(EDV)
分类号:
R587.2; R392.11
DOI:
10.3969/j.issn.1671-7414.2020.01.019
文献标志码:
A
摘要:
目的 探讨血清生长停滞特异基因 6(Growth arrest specific gene 6,Gas6)和血小板反应蛋白-1(thrombospondin-1,TSP-1)水平与糖尿病视网膜病变(diabetic retinopathy,DR)的相关性.方法 选择2017年3月～2019年3月在陕西省咸阳市中心医院和西安市第八医院就诊的152例2型糖尿病(Type 2 diabetes mellitus,T2DM)患者作为研究对象,依据DR分型标准分为增殖性糖尿病视网膜病变(proliferative diabetic retinopathy,PDR)组(45例),非增殖性糖尿病视网膜病变(non-proliferative diabetic retinopathy,NPDR)组(52例)和单纯糖尿病(diabetes mellitus,DM)组(55例)。血清Gas6和TSP-1水平采用酶联免疫吸附法检测。空腹血糖(fasting serum glucose,FSG)采用己糖激酶法测定,糖化血红蛋白A1c(glycocsylated A1c,HbA1c)采用液相色谱法检测。采用彩色多普勒超声仪检测视网膜中央动脉收缩期最大流速(peak systolic velocity,PSV)和舒张末期血流速度(end diastolic velocity,EDV),比较分析以上指标的变化与DR的相关性。结果 血清Gas6在DM组、NPDR组和 PDR组中的水平分别为7.39±1.17,5.14±0.86和4.28±0.79ng/L; 而血清TSP-1在三组中的水平分别为163.96±52.41,113.26±41.07和92.38±35.24ng/L,与DM组比较,血清Gas6和TSP-1水平在NPDR组和 PDR组中显著降低; 其中PDR组降低更显著,以上结果比较的差异均有统计学意义(F=83.37～136.49,均P=000)。在NPDR组和PDR组中,血清Gas6 和TSP-1水平分别有正相关性(r= 0.819,0.837,P均<0.01)。在NPDR组中,Gas6和TSP-1水平分别与FPG和HbA1c水平有负相关性,而与视网膜中央动脉PSV和EDV有正相关性(rGas6=-0.826,-0.809,0.792, 0.778,均P<0.01; rTSP-1= -0.802,-0.789,0.784,0.765,均P<0.01)。在PDR组中,Gas6和TSP-1水平分别与FSG,HbA1c 水平有负相关性,而与视网膜中央动脉PSV和EDV有正相关性rGas6= -0.857,-0.839,0.817,0.812,均P<0.01; rTSP-1=-0.838,-0.824,0.813,0.794,均P<0.01)。结论 分析Gas6和TSP-1与 DR 的相关性可以预测DR的发生,评价DR的病情进展,并为DR发病机制的研究提供新的线索。
Abstract:
Objective To investigate the correlation between the levels of growth arrest specific gene 6(Gas6)and thrombospondin-1(TSP-1)in serum and diabetic retinopathy(DR).Methods 152 patients with type 2 diabetes mellitus(T2DM)who were treated from Xianyang Central Hospital and Xi'an Eighth Hospital in Shaanxi Province between March 2017 and March 2019 were divided into proliferative diabetic retinopathy(PDR: 45 cases)group, non-proliferative diabetic retinopathy(NPDR:52 cases)group and simple diabetes mellitus(DM: 55 cases)group according to DR classification criteria.Serum levels of Gas 6 and TSP-1 were measured by enzyme-linked immunosorbent assay.Fasting serum glucose(FSG)was measured by hexokinase. Glycated hemoglobin A1c(HbA1c)was detected by liquid chromatography.Maximum systolic flow of central retinal artery was measured by color doppler ultrasound.The correlation between DR and the changes of the above indicators was compared and analyzed.Results The serum levels of Gas6 in DM group, NPDR group and PDR group were 7.39±1.17,5.14±0.86 and 4.28±0.79ng/L,respectively, while the serum levels of TSP-1 in the three groups were 163.96±52.41,113.26±41.07 and 92.38±35.24ng/L,respectively. Compared with DM group, the serum levels of Gas6 and TSP-1 were significantly lower in NPDR group and PDR group,especially in PDR group.The differences between the above results were statistically significant(F=83.37～136.49,all P=0.000).In NPDR group and PDR group,the serum levels of Gas6 and TSP-1 were positively correlated(r=0.819,0.837,all P<0.01).In NPDR group, Gas6 and TSP-1 levels were negatively correlated with the levels of FPG and HbA1c, respectively, and were positively correlated with PSV and EDV of central retinal artery(rGas6=-0.826,-0.809, 0.792,0.778, all P<0.01; rTSP-1=-0.802,-0.789, 0.784, 0.765,all P<0.01).In PDR group,levels of Gas6 and TSP-1 were negatively correlated with FPG, HbA1c,respectively, and were positively correlated with PSV and EDV of central retinal artery(rGas6=-0.857,-0.839,0.817,0.812,all P<0.01; rTSP-1=-0.838,-0.824,0.813,0.794, all P<0.01).Conclusion Analysis of the correlation between Gas6 as well as TSP-1 and DR can predict the occurrence of DR,evaluate the progress of DR,and provide new clues for the study of the pathogenesis of DR.

参考文献/References:

[1] 季雄娟,邵静,陆胜.糖化血红蛋白及糖化清蛋白水平对糖尿病视网膜病变的诊断价值分析[J]. 现代检验医学杂志,2018,33(5):77-81. JI Xiongjuan, SHAO Jing, LU Sheng. Diagnostic value of glyocsylated hemoglobin A1c and glycated albumin levels in diabetic retinopany[J].Journal of Modern Laboratory Medicine, 2018,33(5): 77-81.
[2] POPESCU M,BOGDAN C,PINTEA A,et al.Antiangiogenic cytokines as potential new therapeutic targets for resveratrol indiabetic retinopathy[J].Drug Des Devel Ther, 2018,12:1985-1996.
[3] 蔡丽英,罗钢,李保金,等.糖尿病视网膜病变患者血清SDF-1,Gas6水平观察[J].山东医药,2016,56(15):83-85. CAI Liying, LUO Gang, LI Baojin, et al. Observation of serum levels of SDF-1 and Gas6 in patients with diabetic retinopathy[J]. Shandong Medical Journal, 2016, 56(15): 83-85.
[4] 刘贝贝,阳琰,李琪,等.2型糖尿病视网膜病变患者血清25羟维生素D、血清趋化素、血管内皮细胞生长因子及缺血性修饰蛋白水平的变化及其意义[J].中国糖尿病杂志,2018,26(3):197-200. LIU Beibei, YANG Yan, LI Qi, et al. Levels and significance of serum 25-(OH)D, chemerin, VEGF and IMA in patients with type 2 diabetic retinopathy [J].Chinese Journal of Diabetes, 2018,26(3): 197-200.
[5] SHAN Kun,LIU Chang, LIU Baihui, et al.Circularnoncoding RNA HIPK3 mediates retinal vascular dysfunction in diabetes mellitus[J].Circulation.2017,136(17):1629-1642.
[6] 吴志美,喻海忠,袁建芬,等.ADAMTS13 和 TSP-1 在糖尿病视网膜病变的意义[J].检验医学与临床,2017,14(19):2880-2881,2884. WU Zhimei,YU Haizhong,YUAN Jianfen,et al.Significance of ADAMTS13 and TSP-1 in diabetic patients[J].Laboratory Medicine and Clinic, 2017, 14(19): 2880-2881,2884.
[7] 王云枫,连续,郭常秀,等.糖尿病视网膜病变患者血清血管内皮生长因子及其受体2和血小板反应性蛋白-1的表达[J].中国老年学杂志,2017,37(7):1651-1652. WANG Yunfeng, LIAN Xu,GUO Changxiu,et al. Expression of vascular endothelial growth factor and its receptor 2 and platelet reactive protein-1 in serum in patients with diabetic retinopathy [J].Chinese Journal of Gerontology, 2017, 37(7): 1651-1652.
[8] 陈梅,才让,祁蕙燕,等.护网明目汤对糖尿病视网膜病变患者血流动力学及血清ICAM-1,ET-1变化的临床观察[J].北京中医药大学学报,2018,41(7): 599-604. CHEN Mei,CAI Rang,QI Huiyan,et al.Influence of Hu wang Mingmu Tang on hemodynamics and serum ICAM-1 and ET-1 changes in patients with diabetic retinopathy [J].Journal of Beijing University of Traditional Chinese Medicine, 2018,41(7): 599-604.
[9] 叶丽燕,关林波,范平,等.重度子痫前期患者GAS6基因834+7G/A和+1 332C/T多态性的研究[J].中华医学遗传学杂志,2017,34(1):45-49. YE Liyan, GUAN Linbo, FAN Ping, et al. Association study between 834+7G/A and+1 332C/T polymorphism in the growth arrest specific gene 6 and risk of severe preeclampsia in Chinese population [J].Chinese Journal of Medical Genetics, 2017,34(1): 45-49.
[10] 王智勇,刘丽萍,高永峰.糖尿病视网膜病变患者血清 Gas6,SDF-1α和 SDF-1变化及意义[J].国际眼科杂志,2017, 17(5):861-864. WANG Zhiyong, LIU Liping,GAO Yongfeng.Expression changes and significance of Gas6, SDF-1α and SDF-1β in serum in patients with diabetic retinopathy [J]. International Eye Science,2017, 17(5): 861-864.

相似文献/References:

[1]张曙晴a,李洪彬b,张骆军a,等.2型糖尿病患者糖化血红蛋白水平与胰岛素分泌及胰岛素抵抗的关系[J].现代检验医学杂志,2016,31(02):108.[doi:10.3969/j.issn.1671-7414.2016.02.032]
　ZHANG Shu-qinga,LI Hong-binb,ZHANG Luo-juna,et al.Correlation between Insulin Secretion,Insulin Resistance and Glycosylated Hemoglobin Levels in Patients with Type 2 Diabetic[J].Journal of Modern Laboratory Medicine,2016,31(01):108.[doi:10.3969/j.issn.1671-7414.2016.02.032]
[2]蔡加炉,王 成,汪俊军.循环微小核糖核酸与2型糖尿病的关系[J].现代检验医学杂志,2015,30(03):5.[doi:10.3969/j.issn.1671-7414.2015.03.002]
　CAI Jia-lu,WANG Cheng,WANG Jun-jun.Relationship between Circulating miRNA and Type 2 Diabetes[J].Journal of Modern Laboratory Medicine,2015,30(01):5.[doi:10.3969/j.issn.1671-7414.2015.03.002]
[3]段发兰a,李亚新b,杨飞翔a,等.2型糖尿病患者血清铁代谢相关指标检测意义[J].现代检验医学杂志,2015,30(03):151.[doi:10.3969/j.issn.1671-7414.2015.03.046]
　Study on Iron Metabolism in Type Diabetes Patients.Study on Iron Metabolism in Type 2 Diabetes Patients[J].Journal of Modern Laboratory Medicine,2015,30(01):151.[doi:10.3969/j.issn.1671-7414.2015.03.046]
[4]吴晓康,马超峰,余鹏博,等.2型糖尿病患者肠道菌群两种分子指纹图谱分析研究[J].现代检验医学杂志,2015,30(04):24.[doi:10.3969/j.issn.1671-7414.2015.04.006]
　WU Xiao-kang,MA Chao-feng,YU Peng-bo,et al.Analysis of Intestinal Microbiota of Type 2 Diabetes Patients of by Two Fingerprint Technologies[J].Journal of Modern Laboratory Medicine,2015,30(01):24.[doi:10.3969/j.issn.1671-7414.2015.04.006]
[5]张阳东a,陈洁b,楚瑞雪,等.2型糖尿病患者低密度脂蛋白水平及其受体基因表达水平研究[J].现代检验医学杂志,2015,30(05):97.[doi:10.3969/j.issn.1671-7414.2015.05.029]
　ZHANG Yang-donga,CHEN Jieb,CHU Rui-xue,et al.Study on Low-density Lipoprotein Levels and It’s Receptor Genes Expression Levels in Type 2 Diabetes Mellitus Patients[J].Journal of Modern Laboratory Medicine,2015,30(01):97.[doi:10.3969/j.issn.1671-7414.2015.05.029]
[6]王丹,王养维,李辉,等.2型糖尿病患者动脉粥样硬化病变与血清CTRP3和CTRP9水平的相关性研究[J].现代检验医学杂志,2015,30(06):20.[doi:10.3969/j.issn.1671-7414.2015.06.006]
　WANG Dan,WANG Yang-wei,LI Hui,et al.Correlation Research on Atherosclerotic Lesions with Serum CTRP3，CTRP9 Level in Patients with Type 2 Diabetic[J].Journal of Modern Laboratory Medicine,2015,30(01):20.[doi:10.3969/j.issn.1671-7414.2015.06.006]
[7]李全双,邓演超,吴 燕,等.初诊2型糖尿病患者综合干预后血栓前状态分子标志物的变化[J].现代检验医学杂志,2015,30(02):36.[doi:10.3969/j.issn.1671-7414.2015.02.011]
[8]叶永红,陈月兰,詹禧奎,等.联合检测Ficolin-3,TRF及hs-CRP对2型糖尿病早期诊断价值[J].现代检验医学杂志,2016,31(04):74.[doi:10.3969/j.issn.16717-414.2016.04.019]
　YE Yong-hong,CHEN Yue-lan,ZHAN Xi-kui,et al.Early Diagnostic Value of Combined Detection of Ficolin-3, TRF and hs-CRP for Type 2 Diabetes[J].Journal of Modern Laboratory Medicine,2016,31(01):74.[doi:10.3969/j.issn.16717-414.2016.04.019]
[9]万淑君,王 成,王 静,等.2型糖尿病微血管并发症患者血清miR-16,miR-126和miR-221水平检测及临床意义[J].现代检验医学杂志,2016,31(05):9.[doi:10.3969/j.issn.1671-7414.2016.05.003]
　WAN Shu-jun,WANG Cheng,WANG Jing,et al.Study on Serum Levels of miR-16,miR-126 and miR-221 and Their Clinical Significance in Type 2 Diabetes Patients with or Without Microvascular Complications[J].Journal of Modern Laboratory Medicine,2016,31(01):9.[doi:10.3969/j.issn.1671-7414.2016.05.003]
[10]朱影明,许新强,周 强,等.广东地区2型糖尿病与抵抗素基因位点rs2161490和rs1423096多态性的相关性研究[J].现代检验医学杂志,2017,32(01):48.[doi:10.3969/j.issn.1671-7414.2017.01.014]
　ZHU Ying-ming,XU Xin-qiang,ZHOU Qiang,et al.Correlation Study between Resistin rs2161490 and rs1423096 and Type 2 Diabetes Mellitus[J].Journal of Modern Laboratory Medicine,2017,32(01):48.[doi:10.3969/j.issn.1671-7414.2017.01.014]

备注/Memo

备注/Memo:
作者简介:罗文强(1978-),男,大专学历,主管检验师,研究方向:糖尿病的实验室诊断,E-mail:2123689772@qq.com。 通讯作者:茹平(1984-),男,本科学历,主管检验师,研究方向:糖尿病的实验室诊断,E-mail:3383418063@qq.com。收稿日期:2019-08-14 修回日期:2019-09-30
更新日期/Last Update: 2020-03-30