[1]罗　平,沈开元,卢　娜,等.体外受精 - 胚胎移植（IVF-ET）胚胎培养液中 HLA-G 检测在预测胚胎发育潜能和临床结局中的应用价值研究[J].现代检验医学杂志,2020,35(06):163-166.[doi:doi:10.3969/j.issn.1671-7414.2020.06.040]
　LUO Ping,SHEN Kai-yuan,LU Na,et al.Correlation between HLA-G Detection in (IVF-ET) Embryo Culture Mediumand Embryonic Development Potential and Clinical Outcome Abstract[J].Journal of Modern Laboratory Medicine,2020,35(06):163-166.[doi:doi:10.3969/j.issn.1671-7414.2020.06.040]
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体外受精 - 胚胎移植（IVF-ET）胚胎培养液中 HLA-G 检测在预测胚胎发育潜能和临床结局中的应用价值研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第35卷
期数:
2020年06期
页码:
163-166
栏目:
检验与临床
出版日期:
2020-12-30

文章信息/Info

Title:
Correlation between HLA-G Detection in (IVF-ET) Embryo Culture Mediumand Embryonic Development Potential and Clinical Outcome Abstract
文章编号:
1671-7414（2020）06-163-05
作者:
罗　平; 沈开元; 卢　娜; 罗江霞; 黄　芬; 刘刚毅
（柳州市人民医院生殖医学科，广西柳州 545006）
Author(s):
LUO Ping; SHEN Kai-yuan; LU Na; LUO Jiang-xia; HUANG Fen; LIU Gang-yi
(Reproductive Medicine Center, Liuzhou People’s Hospital, Guangxi Liuzhou 545006,China)
关键词:
体外受精 - 胚胎移植; 胚胎培养上清液; 可溶性人类白细胞抗原 G; 发育潜能; 临床结局
分类号:
R394-33
DOI:
doi:10.3969/j.issn.1671-7414.2020.06.040
文献标志码:
A
摘要:
目的　探讨培养液中可溶性人类白细胞抗原 G(HLA-G) 表达水平在预测胚胎发育潜能、评估临床结局中的应用价值。方法　选取 2017 年 12 月 ~2019 年 8 月在柳州市人民医院生殖医学科接受体外受精 - 胚胎移植（IVF-ET）治疗的不孕不育夫妇共 156 对，收集其胚胎培养第三天（D3）或第五天（D5）的培养上清液，检测培养液中 HLA-G 浓度。根据移植临床结局分为临床妊娠组和未妊娠组，根据移植胚胎培养液 HLA-G 浓度按 D3 卵裂期胚胎≥ 2.5U/ml 或D5 囊胚≥ 6.8U/ml 分为高浓度组和低浓度组分别进行比较，同时对 D3 胚胎继续培养形成囊胚情况及不同级别囊胚培养液 HLA-G 含量进行比较分析。结果　临床妊娠组和未妊娠组间的平均年龄、不孕年限、抗苗勒管激素（AMH）、子宫内膜厚度、平均获卵数、受精率、优质胚胎率比较差异均无统计学意义（均 P ＞ 0.05），HLA-G 浓度分别为 7.38±4.12U/ml 和 2.46±1.69 U/ml，两组比较差异有统计学意义（t=3.628，P ＜ 0.01）；形成优质囊胚、可用囊胚和不可用囊胚培养液中的 HLA-G 浓度分别为 12.36±5.27，8.15±3.69 和 5.62±3.10 U/ml，三组比较差异有统计学意义（t=2.856，P＜ 0.05）；HLA-G 高浓度组临床妊娠率和胚胎种植率分别为 69.41%（59/85）和 49.60%（62/125），均高于 HLA-G 低浓度组 46.05%（35/76）和 26.78%（30/112），差异有统计学意义 (χ 2 =9.011，12.946，均 P ＜ 0.01），两组之间的生化妊娠率和流产率比较差异无统计学意义（χ 2 =0.527，0.368，均 P ＞ 0.05）。结论　检测胚胎培养液中 HLA-G 水平可作为胚胎发育潜能的预测指标，联合形态学评分进行胚胎移植可获得较好的临床结局。

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更新日期/Last Update: 2020-12-20