[1]王卫国,王伟伟,马黎丽.儿童传染性单核细胞增多症患者外周血T细胞、血浆PD-1和Tim-3水平表达检测的临床意义[J].现代检验医学杂志,2021,36(02):32-34.[doi:doi:10.3969/j.issn.1671-7414.2021.02.008]
 WANG Wei-guo,WANG Wei-wei,MA Li-li.Clinical Significance of Detecting PD-1 and Tim-3 Expression of T Cells and Plasma in Peripheral Blood from Children with Infectious Mononucleosis[J].Journal of Modern Laboratory Medicine,2021,36(02):32-34.[doi:doi:10.3969/j.issn.1671-7414.2021.02.008]
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儿童传染性单核细胞增多症患者外周血T细胞、血浆PD-1和Tim-3水平表达检测的临床意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第36卷
期数:
2021年02期
页码:
32-34
栏目:
论 著
出版日期:
2021-04-30

文章信息/Info

Title:
Clinical Significance of Detecting PD-1 and Tim-3 Expression of T Cells and Plasma in Peripheral Blood from Children with Infectious Mononucleosis
文章编号:
1671-7414(2021)02-032-04
作者:
王卫国王伟伟马黎丽
(阜阳市人民医院检验科,安徽阜阳 236001)
Author(s):
WANG Wei-guo WANG Wei-wei MA Li-li
(Department of Clinical Laboratory, Fuyang People’s Hospital, Anhui Fuyang 236001, China)
关键词:
传染性单核细胞增多症程序性死亡分子1T细胞免疫球蛋白及黏蛋白分子3
分类号:
R512.7;R392.11
DOI:
doi:10.3969/j.issn.1671-7414.2021.02.008
文献标志码:
A
摘要:
目的 探讨儿童传染性单核细胞增多症(infectious mononucleosis,IM)患者外周血T细胞、血浆PD-1和Tim-3表达检测的临床意义。方法 采集67例IM患儿和20例健康对照者的外周血,流式细胞仪检测PD-1和Tim-3在CD4+和CD8+ T细胞上的表达,ELISA法检测血浆PD-1和Tim-3的浓度。结果 与对照组比较,IM组CD4+ T和CD8+ T细胞上PD-1和Tim-3表达百分比、血浆PD-1和Tim-3浓度均明显增加(t=7.646~23.161,均P<0.01)。ROC曲线分析,血浆PD-1和Tim-3诊断IM的界值分别为1.24 ng/ml和0.44 ng/ml,敏感度分别为62.7%和65.7%,特异度分别为85.0%和75.0%。结论 IM患儿的免疫抑制与PD-1及Tim-3表达增高有关,血浆PD-1和Tim-3浓度对诊断IM具有一定的价值。

参考文献/References:

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[1]左俊丽,白巨星.支气管哮喘患者外周血PD-1,IFN-γ,IL-4与Th细胞及T细胞亚群水平表达的临床意义研究[J].现代检验医学杂志,2021,36(06):47.[doi:10.3969/j.issn.1671-7414.2021.06.009]
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备注/Memo

备注/Memo:
基金项目:阜阳市卫生健康委科研课题(编号:2019-93)。
作者简介:王卫国(1974-),男,专科,副主任技师,研究方向:血液病学,E-mail:wangweiguo388@163.com。
更新日期/Last Update: 2021-04-10