[1]李　凌,王　成,陈小伟,等.2型糖尿病微血管并发症患者血清miR-152，β2-GPI/ox-LDL和β2-GPⅠ-Lp(a)水平联合检测的价值研究[J].现代检验医学杂志,2021,36(02):61-65.[doi:doi:10.3969/j.issn.1671-7414.2021.02.015]
　LI Ling,WANG Cheng,CHEN Xiao-wei,et al.Clinical Value of Combined Retention of Serum miR-152, β2-GPI/ox-LDL and β2-GPⅠ-Lp(a) in the Type 2 Diabetes Patients with Microvascular Complications[J].Journal of Modern Laboratory Medicine,2021,36(02):61-65.[doi:doi:10.3969/j.issn.1671-7414.2021.02.015]
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2型糖尿病微血管并发症患者血清miR-152，β2-GPI/ox-LDL和β2-GPⅠ-Lp(a)水平联合检测的价值研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第36卷
期数:
2021年02期
页码:
61-65
栏目:
论　著
出版日期:
2021-04-30

文章信息/Info

Title:
Clinical Value of Combined Retention of Serum miR-152, β2-GPI/ox-LDL and β2-GPⅠ-Lp(a) in the Type 2 Diabetes Patients with Microvascular Complications
文章编号:
1671-7414（2021）02-061-05
作者:
李　凌; 王　成; 陈小伟; 等
（1. 南京市六合区人民医院检验科，南京211500；2. 中国人民解放军东部战区总医院检验科，南京210002）
Author(s):
LI Ling;  WANG Cheng;  CHEN Xiao-wei;  et al
(1. Department of Clinical Laboratory，the People’s Hospital of Liuhe in Nanjing, Nanjing 211500, China; 2. Department of Clinical Laboratory，General Hospital of Eastern Theater Commend，PLA，Nanjing 210002, China)
关键词:
2型糖尿病微血管并发症; miR-152; β2-糖蛋白I氧化低密度脂蛋白复合物; β2-糖蛋白I脂蛋白a复合物
分类号:
R587.1；R392.11
DOI:
doi:10.3969/j.issn.1671-7414.2021.02.015
文献标志码:
A
摘要:
目的　探讨血清miR-152，β2-糖蛋白I氧化低密度脂蛋白复合物（β2-GPI/ox-LDL）和β2-糖蛋白I脂蛋白a复合物 [β2-GPⅠ-Lp(a)]水平联合检测对2型糖尿病（type 2 diabetes，T2DM）微血管并发症（type 2 diabetes associated microvascular complications，T2DMC）的诊断价值。方法　选取经临床确诊的T2DM患者79例，T2DMC患者78例及体检健康者78例，应用实时荧光定量PCR技术检测血清miR-152的水平，ELISA法检测血清β2-GPI/ox-LDL和β2-GPⅠ-Lp(a)水平，评价上述指标单独或联合检测对于T2DMC的诊断价值。结果　血清miR-152，β2-GPI/ox-LDL和β2-GPⅠ-Lp(a) 在T2DM患者中分别为44.99（25.71，77.97）fmol/L，0.574（0.376，1.040）U/ml和0.536（0.198, 1.056）U/ml，T2DMC患者分别为65.27（43.7，118.1）fmol/L，0.829（0.591，1.138）U/ml和0.597（0.186，1.139）U/ml，均高于健康对照组[13.68（8.14，21.63）fmol/L， 0.497（0.196，0.677）U/ml和0.156（0.078，0.476）U/ml]，差异有统计学意义（U=616~2 192，均P< 0.05）。ROC曲线显示，血清miR-152，β2-GPI/ox-LDL和β2-GPⅠ-Lp(a)对T2DMC诊断的曲线下面积（AUC）分别为0.899（95% CI：0.847 ~ 0.950），0.785（95% CI：0.716 ~ 0.855）和0.718（95% CI：0.637 ~ 0.798）；三者联合检测对T2DMC的AUC为0.963（95% CI：0.934 ~ 0.992）。Spearman秩相关分析显示，血清miR-152，β2-GPI/ox-LDL和β2-GPⅠ-Lp(a)组合与LDL水平呈正相关（r=0.188，P < 0.05）。结论　血清miR-152联合β2-GP1-ox-LDL，β2-GP1-Lp(a)对于T2DMC诊断具有较高的灵敏度和特异度，可有效弥补单指标检测不足，是T2DMC潜在的辅助诊断标志物。

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备注/Memo

备注/Memo:
作者简介：李凌（1976-），女，本科，学士学位，副主任技师，研究方向：生化检验，E-mail: liling011212@126.com。
通讯作者：陈小伟（1984-），男，硕士研究生，副主任技师，研究方向：临床生化检验研究，E-mail: cxw025@qq.com。
更新日期/Last Update: 2021-04-10