[1]沈丹丹,刘　妍,胡　娟,等.一例无毒力白喉棒状杆菌携带者的临床特征和病原学诊断分析[J].现代检验医学杂志,2021,36(04):132-135.[doi:10.3969/j.issn.1671-7414.2021.04.028]
　SHEN Dan-dan,LIU Yan,HU Juan,et al.Clinical Characteristics and Etiological Diagnosis of A Case of NontoxicCorynebacterium Diphtheria Carrier[J].Journal of Modern Laboratory Medicine,2021,36(04):132-135.[doi:10.3969/j.issn.1671-7414.2021.04.028]
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一例无毒力白喉棒状杆菌携带者的临床特征和病原学诊断分析()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第36卷
期数:
2021年04期
页码:
132-135
栏目:
研究简报·实验技术
出版日期:
2021-07-31

文章信息/Info

Title:
Clinical Characteristics and Etiological Diagnosis of A Case of NontoxicCorynebacterium Diphtheria Carrier
文章编号:
1671-7414（2021）04-132-05
作者:
沈丹丹; 刘　妍; 胡　娟; 袁应华
（同济大学附属第十人民医院检验科，上海 200072）
Author(s):
SHEN Dan-dan; LIU Yan; HU Juan; YUAN Ying-hua
(Department of Clinical Laboratory, the Tenth People’s Hospital of Tongji University，Shanghai 200072, China)
关键词:
白喉棒状杆菌; 质谱; 宏基因组测序; 毒力基因
分类号:
R378.71；R446.5
DOI:
10.3969/j.issn.1671-7414.2021.04.028
文献标志码:
A
摘要:
目的　对上海市某医院收治的1 例携带白喉棒状杆菌的病例进行病原学诊断分析，为白喉的感控提供临床依据。方法　采集患者痰液进行培养及质谱鉴定、涂片染色鉴定、宏基因组测序（metagenomic sequencing, mNGS），纯培养物行16S rDNA 基因测序进行病原学诊断，同时采用PCR 方法检测白喉棒状杆菌毒力基因toxA 和toxB 表达情况。结果　患者痰液培养48h 后分离到单一灰白色菌落，经质谱鉴定、涂片镜下及16S rDNA 鉴定均提示为白喉棒状杆菌，mNGS 提示白喉棒状杆菌较高序列数，PCR 结果显示toxA 和toxB 毒力基因为阴性。结论　该病例为白喉棒状杆菌携带患者，虽为无毒力感染者，仍当引起重视。

参考文献/References:

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备注/Memo

备注/Memo:
作者简介：沈丹丹（1987-），女，硕士，主管技师，研究方向：细菌耐药机制，E-mail：sdd2832907@163.com。
通讯作者：袁应华（1965-），男，本科，副主任技师，研究方向：细菌耐药机制，E-mail：yuan_yinghua@126.com。
更新日期/Last Update: 1900-01-01