[1]周　娟,竺　青,孙桂芝,等.单采献血者IL-28b基因多态性与隐匿性HBV感染相关性研究[J].现代检验医学杂志,2021,36(05):8-11.[doi:10.3969/j.issn.1671-7414.2021.05.002]
　ZHOU Juan,ZHU Qing,SUN Gui-zhi,et al.Study on the Correlation between IL-28b Gene Polymorphism andOccult HBV Infection in Platelet Donors[J].Journal of Modern Laboratory Medicine,2021,36(05):8-11.[doi:10.3969/j.issn.1671-7414.2021.05.002]
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单采献血者IL-28b基因多态性与隐匿性HBV感染相关性研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第36卷
期数:
2021年05期
页码:
8-11
栏目:
论　著
出版日期:
2021-10-14

文章信息/Info

Title:
Study on the Correlation between IL-28b Gene Polymorphism andOccult HBV Infection in Platelet Donors
文章编号:
1671-7414（2021）05-008-05
作者:
周　娟1; 竺　青1; 孙桂芝1; 张红卫1; 郭成山2; 贺韦东1; 陈　敏1
（1. 山东省血液中心机采科，济南 250014；2. 深圳市宝安区人民医院，广东深圳518101）
Author(s):
ZHOU Juan1;  ZHU Qing1;  SUN Gui-zhi1;  ZHANG Hong-wei1;  GUO Cheng-shan2;  HE Wei-dong1;  CHEN Min1
（1. Department of Mechanical Collection, Shandong Blood Center, Jinan 250014, China;2. People’s Hospital of Bao’an District, Guangdong Shenzhen 518101, China）
关键词:
单采献血者; 隐匿性乙型肝炎病毒感染; 白细胞介素-28b; 基因多态性
分类号:
R457.1；R512.62
DOI:
10.3969/j.issn.1671-7414.2021.05.002
文献标志码:
A
摘要:
目的　研究单采献血者白细胞介素-28b（IL-28b）基因多态性与隐匿性乙型肝炎病毒（HBV）感染（OBI）相关性。方法　选择2016年1月～2019年12月山东省血液中心成功捐献血小板HbsAg（-）、核酸检测（NAT）无反应的献血者1 158例为研究对象，提取献血者血液DNA，采用直接测序法进行IL-28b基因分型，cobas HBV定量核酸检测试剂盒检测血液HBV-DNA载量，Logistic回归分析IL-28b基因rs8099917位点、rs12979860位点多态性与OBI的关系。结果　与非OBI比较，OBI献血者IL-28b基因rs8099917位点G等位基因频率、TG+GG基因型频率显著降低，T等位基因频率、TT基因型频率显著增加（χ2=22.137，22.163，均P<0.01）；rs12979860位点T等位基因频率、CT+TT基因型频率显著降低，C等位基因频率、CC基因型频率显著增加（χ2=16.378，19.091，均P<0.01）。OBI献血者rs8099917位点，与TT基因型比较，TG+GG基因型患者HBV-DNA载量显著降低（t=5.257，P<0.01）；rs12979860位点，与CC基因型比较，CT+TT基因患者HBV-DNA载量显著降低（t=17.398，P<0.01），以上比较差异均有统计学意义。Logistic回归分析结果显示，rs8099917位点，与TT基因型比较，TG+GG基因型可显著降低OBI发生风险（95% CI：0.288~0.843，P<0.05） ；rs12979860位点，与CC基因型比较，CT+TT基因型可显著降低OBI发生风险（95% CI ：0.207~0.761，P<0.05）。结论　单采献血者OBI可能与IL-28b基因rs8099917位点、rs12979860位点多态性有关。
Abstract:
Objective　To study the correlation between interleukin-28b (IL-28b) gene polymorphism and occult hepatitis B virus(HBV) infection (OBI) in platelet donors. Methods　From January 2016 to December 2019, 1 158 platelet donors fromshandong Blood Center who successfully donated platelets with HBsAg (-) and had no response to nucleic acid test (NAT) wereselected as the study subjects, DNA was extracted from blood donors, IL-28b was genotyped by direct sequencing, cobas HBVquantitative nucleic acid detection kit was used to detect HBV-DNA load in blood, and Logistic regression was used to analyzethe relationships between the polymorphisms of rs8099917, rs12979860 loci on IL-28b gene and OBI. Results　Compared withnon OBI, the G allele frequency and TG + GG genotype frequency at rs8099917 locus on IL-28b gene in OBI donors weresignificantly lower, while the T allele frequency and TT genotype frequency were significantly higher (χ2=22.137, 22.163, allP<0.01). T allele frequency and CT + TT genotype frequency of rs12979860 were significantly lower, while C allele frequencyand CC genotype frequency were significantly higher (χ2=16.378, 19.091, all P<0.01). At rs8099917 locus of OBI donors, HBVDNAload of TG + GG genotype was significantly lower than that of TT genotype (t=5.257, P<0.01). At rs12979860 locus, HBVDNAload of CT + TT genotype was significantly lower than that of CC genotype(t=17.398, P<0.01), and the above differenceswere statistically significant. Logistic regression analysis showed that, at rs8099917 locus, compared with TT genotype, TG + GGgenotype could significantly reduced the risk of OBI (95% CI:0.288~0.843, P<0.05). At rs12979860 locus, compared with CCgenotype, compared with CC genotype, CT + TT genotype could significantly reduced the risk of OBI (95% CI: 0.207~0.761, P<0.05). Conclusion　 OBI of platelet donors may be related to the polymorphisms of rs8099917 andrs12979860 loci on IL-28b gene.

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备注/Memo

备注/Memo:
基金项目：国家自然科学基金（NO.81270577）。
作者简介：周娟（1972-）女，本科，副主任护师，研究方向：机采血小板，乙肝，丙肝，艾滋病，E-mail：zj250014@163.com。
通讯作者：陈敏（1977-），女，本科，副主任护师，E-mail:06130117@163.com。
更新日期/Last Update: 1900-01-01