[1]薛玉峰a,张会平a,王　芳b,等.鼻咽癌患者血清miR-144-3p及miR-151-3p的表达水平及临床价值[J].现代检验医学杂志,2021,36(05):65-68.[doi:10.3969/j.issn.1671-7414.2021.05.015]
　XUE Yu-fenga,ZHANG Hui-pinga,WANG Fangb,et al.Expression and Clinical Value of Serum miR-144-3p and miR-151-3pLevels in Patients with Nasopharyngeal Carcinoma[J].Journal of Modern Laboratory Medicine,2021,36(05):65-68.[doi:10.3969/j.issn.1671-7414.2021.05.015]
点击复制

鼻咽癌患者血清miR-144-3p及miR-151-3p的表达水平及临床价值()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第36卷
期数:
2021年05期
页码:
65-68
栏目:
论　著
出版日期:
2021-10-14

文章信息/Info

Title:
Expression and Clinical Value of Serum miR-144-3p and miR-151-3pLevels in Patients with Nasopharyngeal Carcinoma
文章编号:
1671-7414（2021）05-065-04
作者:
薛玉峰a; 张会平a; 王　芳b; 郝宗生a
( 涿州市医院 a. 耳鼻喉科；b. 药剂科，河北涿州 072750)
Author(s):
XUE Yu-fenga;  ZHANG Hui-pinga;  WANG Fangb;  HAO Zong-shenga
（a.Department of Otolaryngology；b.Departmentof Pharmacy, Zhuozhou City Hospital, Hebei Zhuozhou 072750, China）
关键词:
鼻咽癌; 微小核糖核酸-144-3p; 微小核糖核酸-151-3p
分类号:
R739.63；R730.43
DOI:
10.3969/j.issn.1671-7414.2021.05.015
文献标志码:
A
摘要:
目的　探讨鼻咽癌患者血清miR-144-3p及miR-151-3p的表达水平及临床价值。方法　选取2016年1月～2020年7月涿州市医院收治的106例鼻咽癌患者和50例健康对照组作为研究对象，采用实时定量PCR法检测miR-144-3p及miR-151-3p表达水平。应用受试者工作特征（ROC）曲线分析miR-144-3p及miR-151-3p诊断鼻咽癌的价值。Pearson相关分析鼻咽癌患者血清miR-144-3p与miR-151-3p表达水平的相关性。结果　鼻咽癌组血清miR-144-3p（2.36±1.05 vs 0.82±0.27）及miR-151-3p（3.58±1.73 vs 0.97±0.36）表达水平均明显高于对照组，差异有统计学意义（均P <0.001）。鼻咽癌患者血清miR-144-3p及miR-151-3p表达水平升高与临床分期高、分化程度低、淋巴结转移及远处转移有关（t=5.824~19.307，均P <0.01）。ROC曲线显示， miR-144-3p为1.61及miR-151-3p为2.25是诊断鼻咽癌的最佳截断值，二者联合诊断鼻咽癌的曲线下面积（0.937，95%CI：0.875~0.99）最大，其敏感度和特异度为95.3%和87.0%。鼻咽癌患者血清miR-144-3p表达水平与miR-151-3p呈正相关（r=0.872，P<0.001）。结论　鼻咽癌患者血清miR-144-3p及miR-151-3表达水平明显升高，二者联合诊断鼻咽癌的价值较高。
Abstract:
Objective　To investigate the expression level and clinical value of miR-144-3p and miR-151-3p in patients withnasopharyngeal carcinoma. Methods　The 106 patients with nasopharyngeal carcinoma and 50 healthy controls who wereadmitted to Zhuozhou City Hospital from January 2016 to July 2020 were selected as the research subjects. The expressionlevels of miR-144-3p and miR-151-3p were detected by real-time quantitative PCR. The levls of miR-144-3p and miR-151-3pin the patients was analyzed by ROC curve in the diagnosis of nasopharyngeal carcinoma. Pearson correlation analysis was usedto analyze the correlation between serum miR-144-3p expression level and miR-151-3p level in patients with nasopharyngealcarcinoma. Results　The expression levels of serum miR-144-3p（2.36±1.05 vs 0.82±0.27） and miR-151-3p（3.58±1.73vs 0.97±0.36） in nasopharyngeal carcinoma group were significantly higher than those in control group, the differences werestatistically significant (all P< 0.001). The increased expression levels of serum miR-144-3p and miR-151-3p were associatedwith high clinical stage, low differentiation, lymph node metastasis and distant metastasis (t=5.824~19.307, all P< 0.01). ROCcurve showed that the best cut-off values of miR-144-3p and miR-151-3p in the diagnosis of nasopharyngeal carcinoma were 1.61and 2.25, respectively. The area under the curve (0.937, 95% CI: 0.875~0.99) was the largest for the two combined diagnosis ofnasopharyngeal carcinoma, and the sensitivity and specificity were 95.3% and 87.0%, respectively. The expression levels of serummiR-144-3p was positively correlated with miR-151-3p (r=0.872, P< 0.001). Conclusion　The expression levels of serum miR-144-3p and miR-151-3 in nasopharyngeal carcinoma patients were significantly increased, and the two combined diagnosis value ofnasopharyngeal carcinoma was relatively high.

参考文献/References:

[1] WU Sangang, ZHANG Wenwen, WANG Jun, et al. The1-year mortality after radiotherapy for nasopharyngealcarcinoma: a population-based analysis[J]. FutureOncology (London, England), 2019, 15(29): 3357-3365.
[2] TAN Luping, TAN G W, SIVANESAN V M, et al.Systematic comparison of plasma EBV DNA, anti‐EBV antibodies and miRNA levels for early detectionand prognosis of nasopharyngeal carcinoma[J].International Journal of Cancer, 2020, 146(8): 2336-2347.
[3] WANG Tianyu, WU Jian, WU Yun, et al. A novelmicroRNA-based signature predicts prognosis amongnasopharyngeal cancer patients[J]. ExperimentalBiology and Medicine (Maywood, N.J.), 2021, 246(1):72-83.
[4] LIU Dan, GONG Hao, TAO Zezhang, et al. LncRNAIUR downregulates miR-144 to regulate PTEN innasopharyngeal carcinoma[J]. Archives of Physiologyand Biochemistry, 2020, 126: 1-6.
[5] YUE Chaochi, CHEN Xiaochao, LI Jun, et al. miR-151-3p inhibits proliferation and invasion of coloncancer cell by targeting close homolog of L1[J]. Journalof Biomedical Nanotechnology, 2020, 16(6): 876-884.
[6] WU Lirong, ZHENG Kexiao, YAN Cheng, et al.Genome-wide study of salivary microRNAs as potentialnoninvasive biomarkers for detection of nasopharyngealcarcinoma[J]. BMC Cancer, 2019, 19(1): 843.
[7] TSIAMBAS E, MASTRONIKOLIS N S, FOTIADESP P, et al. Epstein-Barr virus microRNAs innasopharyngeal carcinoma[J]. Pathology OncologyResearch, 2020, 26(2): 1347-1348.
[8] LOU Ning, RUAN Anming, QIU Bin, et al. miR-144-3p as a novel plasma diagnostic biomarker for clearcell renal cell carcinoma[J]. Urologic Oncology, 2017,35(1): 36.e7-36.e14.
[9] LI Xia, LIU Yi, ZHANG Xiangxin, et al. Circular RNAhsa_circ_0000073 contributes to osteosarcoma cellproliferation, migration, invasion and methotrexateresistance by sponging miR-145-5p and miR-151-3p and upregulating NRAS[J]. Aging, 2020, 12(14):14157-14173.
[10] SONG Li, CHEN Lijie, LUAN Qiang, et al. miR-144-3p facilitates nasopharyngeal carcinoma via crosstalkwith PTEN[J]. Journal of Cellular Physiology, 2019,234(10): 17912-17924.
[11] LIU Haibin, CHENG Yin, XU Yaping, et al. Theinhibition of tumor protein p53 by microRNA-151a-3p induced cell proliferation, migration and invasionin nasopharyngeal carcinoma[J]. Bioscience Reports,2019, 39(10): BSR20191357.
[12] LIAN Junsheng, LI Yujie, YU Min. MicroRNA-183and microRNA-141 are potential risk factors for poorprognosis in patients with nasopharyngeal carcinoma[J].Oncology Letters, 2019, 17(1): 1172-1176.
[13] LI Shuna, HANG Lihua, MA Yongming, et al.Distinctive microRNA expression in early stagenasopharyngeal carcinoma patients[J]. Journal ofCellular and Molecular Medicine, 2016, 20(12): 2259-2268.
[14] WEN Wen, MAI Shijuan, LIN Huanxin, et al.Identification of two microRNA signatures inwhole blood as novel biomarkers for diagnosis ofnasopharyngeal carcinoma[J]. Journal of TranslationalMedicine, 2019, 17(1): 186.
[15] ZHANG Huo, ZOU Xuan, WU Lirong, et al.Identification of a 7-microRNA signature in plasma aspromising biomarker for nasopharyngeal carcinomadetection[J]. Cancer Medicine, 2020, 9(3): 1230-1241.

相似文献/References:

[1]李 璐,梁 辉a,陈群蓉b.鼻咽癌组织中SYK基因启动子区的甲基化分析[J].现代检验医学杂志,2015,30(02):39.[doi:10.3969/j.issn.1671-7414.2015.02.012]
　LI Lu,LIANG Huia,CHEN Qun-rongb.Methylation of SYK Gene Promoter Region in Nasopharyngeal Carcinoma[J].Journal of Modern Laboratory Medicine,2015,30(05):39.[doi:10.3969/j.issn.1671-7414.2015.02.012]
[2]彭玉凤,张扬南,黄常洪,等.鼻咽癌患者血清同型半胱氨酸水平与临床病理特征的相关性分析[J].现代检验医学杂志,2019,34(02):115.[doi:10.3969/j.issn.1671-7414.2019.02.029]
　PENG Yu-feng,ZHANG Yang-nan,HUANG Chang-hong,et al.Correlation Analysis between Serum Homocysteine Level ofNasopharyngeal Carcinoma Patients and Clinical Pathological Features[J].Journal of Modern Laboratory Medicine,2019,34(05):115.[doi:10.3969/j.issn.1671-7414.2019.02.029]
[3]张玲,刘杰,王臣玉,等.EB 病毒Rta 优势表位抗原的克隆表达及在应用ELISA 诊断鼻咽癌中的价值[J].现代检验医学杂志,2020,35(05):1.[doi:10.3969/j.issn.1671-7414.2020.05.001]
　ZHANG Ling,LIU Jie,WANG Chen-yu,et al.Cloning and Expression of Dominant Epitope Antigen of Epstein-Barr Virus Rta Protein and Its Application in the Diagnosis of Nasopharyngeal Carcinoma by ELISA[J].Journal of Modern Laboratory Medicine,2020,35(05):1.[doi:10.3969/j.issn.1671-7414.2020.05.001]
[4]潘国刚,杨碧秀.鼻咽癌不同临床分期中白细胞介素水平表达的最新研究进展[J].现代检验医学杂志,2021,36(05):184.[doi:10.3969/j.issn.1671-7414.2021.05.040]
　PAN Guo-gang,YANG Bi-xiu.Latest Research Progress on the Expression Mechanism of Interleukin inNasopharyngeal Carcinoma Different Clinical Staging[J].Journal of Modern Laboratory Medicine,2021,36(05):184.[doi:10.3969/j.issn.1671-7414.2021.05.040]
[5]胡鹏刚,张昌明.枸杞皂苷通过调控Suv39H1/JAK2/STAT3通路对鼻咽癌细胞增殖、侵袭和凋亡的影响[J].现代检验医学杂志,2021,36(06):27.[doi:10.3969/j.issn.1671-7414.2021.06.006]
　HU Peng-gang,ZHANG Chang-ming.Lycium Barbarum Saponins Affect Proliferation, Invasion and Apoptosis of Nasopharyngeal Carcinoma Cells by Regulating the Suv39H1/JAK2/STAT3 Pathway[J].Journal of Modern Laboratory Medicine,2021,36(05):27.[doi:10.3969/j.issn.1671-7414.2021.06.006]
[6]顾刘雷a,顾 培b,金广浩a,等.鼻咽癌组织中LncRNA CTBP1-AS2 和miR-140-5p 水平表达与放疗疗效及预后的关系研究[J].现代检验医学杂志,2024,39(02):23.[doi:10.3969/j.issn.1671-7414.2024.02.005]
　GU Liuleia,GU Peib,JIN Guanghaoa,et al.Relationship between the Expression of LncRNA CTBP1-AS2 and miR-140-5p Levels in Nasopharyngeal Carcinoma Tissues and Radiotherapeutic Effect and Prognosis[J].Journal of Modern Laboratory Medicine,2024,39(05):23.[doi:10.3969/j.issn.1671-7414.2024.02.005]

备注/Memo

备注/Memo:
基金项目：保定市科学计划与发展指导计划项目（18ZF146）。
作者简介：薛玉峰（1980-）, 男，主治医师，本科，主要从事临床耳鼻喉疾病研究。E-mail：xueyufeng2020@163.com。
更新日期/Last Update: 1900-01-01