[1]欧阳航a,陈德波b,骆时木a,等.乳腺癌晚期患者循环肿瘤细胞表型Vimentin mRNA与AKT1 mRNA水平表达对临床疗效评估的价值研究[J].现代检验医学杂志,2021,36(05):73-76.[doi:10.3969/j.issn.1671-7414.2021.05.017]
　OUYANG Hanga,CHEN De-bob,LUO Shi-mua,et al.Value of Circulating Tumor Cell Phenotype Vimentin mRNA and AKT1mRNA Expression in the Evaluation of Clinical Efficacy in Patients withAdvanced Breast Cancer[J].Journal of Modern Laboratory Medicine,2021,36(05):73-76.[doi:10.3969/j.issn.1671-7414.2021.05.017]
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乳腺癌晚期患者循环肿瘤细胞表型Vimentin mRNA与AKT1 mRNA水平表达对临床疗效评估的价值研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第36卷
期数:
2021年05期
页码:
73-76
栏目:
论　著
出版日期:
2021-10-14

文章信息/Info

Title:
Value of Circulating Tumor Cell Phenotype Vimentin mRNA and AKT1mRNA Expression in the Evaluation of Clinical Efficacy in Patients withAdvanced Breast Cancer
文章编号:
1671-7414（2021）05-073-05
作者:
欧阳航a; 陈德波b; 骆时木a; 杨培东b; 吴一波a; 张志珊a
（福建医科大学附属泉州第一医院 a. 检验科；b. 乳腺外科，福建泉州 362000）
Author(s):
OUYANG Hanga;  CHEN De-bob; LUO Shi-mua; YANG Pei-dongb; WU Yi-boa;  ZHANG Zhi-shana
（a.Department of Clinical Laboratory；b. Department of Breast Surgery，the First Hospital of Quanzhou Affiliated toFujian Medical University, Fujian Quanzhou 362000, China）
关键词:
晚期乳腺癌; 循环肿瘤细胞; 波形蛋白; 蛋白激酶B1
分类号:
R737.9；R730.43
DOI:
10.3969/j.issn.1671-7414.2021.05.017
文献标志码:
A
摘要:
目的　探讨乳腺癌晚期患者外周血中循环肿瘤细胞（circulating tumor cells,CTC）表型标志物波形蛋白（Vimentin）mRNA与蛋白激酶B（protein kinase B,AKT1） mRNA表达水平与患者治疗疗效的相关性。方法　收集2018年6月～2020年1月在福建医科大学附属泉州第一医院乳腺外科就诊且初次确诊为晚期乳腺癌的患者共43例，同时选择15例健康体检者作为对照组。每次分别在患者治疗前以及经过2个治疗周期结束后采集外周血标本7.5ml，经差相富集法富集CTC后检测其Vimentin mRNA及AKT1 mRNA的表达情况，并据实体瘤疗效评价标准1.1（response evaluation criteria in solid tumors, RECIST）进行疗效评估。实验所得数据使用SPSS20.0统计软件进行数据分析，GraphPad Prism 7.0软件进行数据的作图。结果　晚期乳腺癌患者Vimentin mRNA和AKT1 mRNA的相对表达量均高于健康对照组（t=2.841，2.480,均P＜0.05）。患者治疗后Vimentin mRNA和AKT1 mRNA相对表达量低于治疗前，差异均有统计学意义（t=2.879，2.282,均P＜0.05），且治疗后Vimentin mRAN表达量下降患者有更高的治疗有效率（P＜0.05）。结论　晚期乳腺癌患者治疗后Vimentin mRNA和AKT1 mRNA表达量均显著降低，且治疗后Vimentin mRNA表达量降低与更高的治疗疗效有关，提示治疗前后Vimentin mRNA的变化可用于评估患者治疗疗效。
Abstract:
Objective　To study the expression of circulating tumor cells phenotype markers Vimentin mRNA and AKT1 mRNAin the peripheral blood of patients with advanced breast cancer and their correlation with disease activity therapeutic. Methods　From June 2018 to January 2020, 43 patients diagnosed as advanced breast cancer were collected from the First Hospital ofQuanzhou Affiliated to Fujian Medical University. At the same time, 15 healthy subjects were selected as the control group.7.5ml of peripheral blood samples were collected before treatment and after two cycles of treatment. Detected the expression ofVimentin mRNA and protein kinase B (AKT1) mRNA after enrichment of CTC by differential enrichment method. Accordingto the response evaluation criteria in solid tumors (RECIST), the therapeutic effect was evaluated. SPSS20.0 statistical softwarewas used for data analysis, and GraphPad Prism 7.0 software was used for data mapping. Results　The relative expressionof Vimentin and AKT1 mRNA in advanced breast cancer group was higher than that in healthy control group, the differenceswere statistically significant（t=2.841,2.480, all P ＜ 0.05）. After treatment of advanced breast cancer patients, the relativeexpression of Vimentin and AKT1 mRNA were significantly reduced（t=2.879,2.282, all P ＜ 0.05）, and the patients withdecreased Vimentin expression had higher therapeutic efficiency (P<0.05) Conclusion　The expression levels of VimentinmRNA and AKT1 mRNA in patients with advanced breast cancer were significantly decreased after treatment, and the decreasedexpression of Vimentin mRNA was related to the higher therapeutic effect, which suggesting that the changes of Vimentin mRNAcould be used to evaluate the therapeutic effect.

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备注/Memo

备注/Memo:
基金项目：福建省卫生计生委中青年骨干人才培养项目（2017-ZQN-77）。
作者简介：欧阳航（1993-），男，硕士研究生，检验师，研究方向：循环肿瘤细胞的应用，E-mail:245706429@qq.com。
通讯作者：张志珊（1973-），女，医学博士，主任技师，研究方向：免疫与分子生物学，E-mail:554882707@qq.com。
更新日期/Last Update: 1900-01-01