[1]康　茹,孟改丽,周雪红.呼吸道感染患儿肠道菌群紊乱与Th17/Treg及其分泌炎性细胞因子免疫平衡的相关性研究[J].现代检验医学杂志,2021,36(06):95-100.[doi:10.3969/j.issn.1671-7414.2021.06.020]
　KANG Ru,MENG Gai-li,ZHOU Xue-hong.Correlation Analysis between Intestinal Flora Disturbance and Immune Balance of Th17/Treg and Its Secretion of Inflammatory Cytokines Children with Respiratory Tract Infection[J].Journal of Modern Laboratory Medicine,2021,36(06):95-100.[doi:10.3969/j.issn.1671-7414.2021.06.020]
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呼吸道感染患儿肠道菌群紊乱与Th17/Treg及其分泌炎性细胞因子免疫平衡的相关性研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第36卷
期数:
2021年06期
页码:
95-100
栏目:
论　著
出版日期:
2021-12-15

文章信息/Info

Title:
Correlation Analysis between Intestinal Flora Disturbance and Immune Balance of Th17/Treg and Its Secretion of Inflammatory Cytokines Children with Respiratory Tract Infection
文章编号:
1671-7414（2021）06-095-06
作者:
康　茹; 孟改丽; 周雪红
（1.西北妇女儿童医院检验科，西安 710061；2.民航西安医院，西安 710082）
Author(s):
KANG Ru;  MENG Gai-li;  ZHOU Xue-hong
（1 .Department of Clinical Laboratory，Northwest Women’s and Children’s Hospital，Xi’an 710006,China；2. Xi’an Hospital of Civil Aviation，Xi’an 710082，China）
关键词:
呼吸道感染; 肠道菌群紊乱; Th17/Treg免疫平衡; 炎性细胞因子
分类号:
R562；R392.11
DOI:
10.3969/j.issn.1671-7414.2021.06.020
文献标志码:
A
摘要:
目的　探讨呼吸道感染患儿肠道菌群紊乱与Th17/Treg免疫平衡及炎性细胞因子的关系。方法　前瞻性选取2019年5月～2020年11月西北妇女儿童医院收治的呼吸道感染患儿135例作为研究观察组，根据肠道菌群紊乱情况分为菌群失调组（n=60）和非菌群失调组（n=75）。另选取同期健康体检儿童100例作为对照。对比各组肠道菌群数量，检测外周血Th17，Treg细胞百分比及血清炎性因子表达水平。Pearson相关性分析肠道菌群与Th17，Treg细胞及炎性因子表达的相关性。结果　菌群失调组乳酸杆菌、双歧杆菌数量及B/E值较非菌群失调组和对照组明显降低，肠球菌、大肠埃希菌数量明显升高，差异有统计学意义（F=13.849~42.449，均 P<0.05）；与对照组相比，非菌群失调组各菌群变化不显著（P>0.05）。与对照组相比，菌群失调组和非菌群失调组Th17%及IL-2，IL-6，IL-17水平明显升高，Treg%及IL-10水平明显降低，其中菌群失调组变化更显著，差异有统计学意义（F=30.483~92.328，均P＜0.05）。Pearson 相关性分析显示，呼吸道感染患儿Th17%及IL-2，IL-6，IL-17水平与乳酸杆菌、双歧杆菌、B/E值呈负相关（r=0.989~0.586，均P＜0.05），与肠球菌、大肠埃希菌呈正相关（r=-0.599~0.800，均P＜0.05）；Treg%及IL-10水平与乳酸杆菌、双歧杆菌、B/E值呈正相关（r =0.672~0.756，均P＜0.05），与肠球菌、大肠埃希菌呈负相关（r= -0.774~-0.684，均P＜0.05）。结论　呼吸道感染患儿肠道菌群紊乱表现为肠道有益菌数量减少，致病菌数量增多；肠道菌群紊乱可能通过诱导机体Th17 /Treg免疫失衡，调控炎性介质分泌，进而参与儿童呼吸道感染的发生发展。
Abstract:
Objective　To investigate the relationship between intestinal flora disturbance and Th17/Treg immune balance and inflammatory cytokines in children with respiratory tract infection. Methods　A total of 135 children with respiratory tract infection admitted to Northwest Women’s and Children’s Hospital from May 2019 to November 2020 were selected as the observation group, and were divided into two groups according to intestinal flora disorder group (n=60) and non-flora disorder group (n=75). Another 100 healthy children in the same period were selected as control. The number of intestinal flora in each group was compared, and the percentages of Th17 and Treg cells in peripheral blood and the expression levels of serum inflammatory factors were detected. Pearson correlation was used to analyze the correlation between intestinal flora and the expression of Th17, Treg cells and inflammatory factors. Results　The number of Lactobacillus and Bifidobacterium and the B/E value in the dysfunctional group were significantly lower than those in the non-dysfunctional group and the control group, while the number of Enterococcus and Escherichia coli were significantly increased, the differences were statistically significant (F=13.849~42.449, all P<0.05). Compared with the control group, the changes of flora in the non-maladjusted group were not significant (P>0.05). Compared with the control group, the levels of Th17% and IL-2, IL-6 and IL-17 were significantly increased in the dysregulated group and non-dysregulated group , while the levels of Treg% and IL-10 were significantly decreased, and the changes in the dysregulated group were more significant, the differences were statistically significant(F=30.483~92.328, all P< 0.05). Pearson correlation analysis showed that the levels of Th17%, IL-2, IL-6 and IL-17 were negatively correlated with Lactobacillus, Bifidobacterium and B/E values（r=0.989~0.586，all P＜0.05）, and positively correlated with Enterococcus and Escherichia coli (r=-0.599~0.800, P < 0.05). Treg% and IL-10 levels were positively correlated with Lactobacillus, Bifidobacterium and B/E values(r=0.672~0.756, all P< 0.05) and negatively correlated with Enterococcus and Escherichia coli (r=-0.774~-0.684, all P< 0.05). Conclusion　The disturbance of intestinal flora in children with respiratory tract infection showed that the number of beneficial bacteria decreased and the number of pathogenic bacteria increased. The disturbance of intestinal flora may be involved in the occurrence and development of respiratory tract infection in children by inducing the immune imbalance of Th17 /Treg in the body and regulating the secretion of inflammatory mediators.

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备注/Memo

备注/Memo:
作者简介：康茹（1989-），女，本科，检验师，研究方向:感染与免疫，E-mail：mgdd210er83@163.com。通讯作者：孟改利（1986-），女，硕士，研究方向:感染与免疫。
更新日期/Last Update: 1900-01-01