[1]叶科导a,刘爱胜,施俊柱b.痛风性关节炎患者血清 IL-1β，TNF-ɑ，hs-CRP水平及与 IL-1β基因启动子区 rs2853550 A/G位点多态性相关性分析[J].现代检验医学杂志,2022,37(01):43-47.[doi:10.3969/j.issn.1671-7414.2022.01.009]
　YE Ke-daoa,LIU Ai-sheng,SHI Jun-zhub.Analysis the Levels of IL-1β,TNF-ɑ,hs-CRP and Its Association with Polymorphism of IL-1βGene Promoter Region rs2853550 A/G Loci in Patients with Gouty Arthritis[J].Journal of Modern Laboratory Medicine,2022,37(01):43-47.[doi:10.3969/j.issn.1671-7414.2022.01.009]
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痛风性关节炎患者血清 IL-1β，TNF-ɑ，hs-CRP水平及与 IL-1β基因启动子区 rs2853550 A/G位点多态性相关性分析()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第37卷
期数:
2022年01期
页码:
43-47
栏目:
论　著
出版日期:
2022-01-15

文章信息/Info

Title:
Analysis the Levels of IL-1β,TNF-ɑ,hs-CRP and Its Association with Polymorphism of IL-1βGene Promoter Region rs2853550 A/G Loci in Patients with Gouty Arthritis
文章编号:
1671-7414（2022）01-043-05
作者:
叶科导1a; 刘爱胜2; 施俊柱1b
（1. 深圳市龙华区中心医院a. 内科；b. 检验科，广东深圳 518110；2. 深圳市龙华区人民医院检验科，广东深圳 518109）
Author(s):
YE Ke-dao1a;  LIU Ai-sheng2;  SHI Jun-zhu1b
（1a. Department of Medicine; 1b. Department of Clinical Laboratory,Shenzhen Longhua District Central Hospital, Guangdong Shenzhen 518110, China; 2. Department of ClinicalLaboratory, Shenzhen Longhua District People’s Hospital, Guangdong Shenzhen 518109, China）
关键词:
痛风性关节炎; 白细胞介素 -1β; 肿瘤坏死因子; 超敏 C反应蛋白; 单核苷酸多态性
分类号:
R684.3；R392.11
DOI:
10.3969/j.issn.1671-7414.2022.01.009
文献标志码:
A
摘要:
目的　分析深圳地区痛风性关节炎（ gouty arthritis，GA）患者血清白细胞介素 -1β（interleukin-1β，IL-1β）、肿瘤坏死因子（ tumor necrosis factor，TNF-ɑ）、超敏 -C反应蛋白（ hypersensitive C-reactive protein，hs-CRP）水平及与 IL-1β基因启动子区 rs2853550 A/G位点多态性相关性。方法　收集 GA确诊患者 216例作为 GA组，同时选取 153例健康体检人群作为对照组。分别检测血清 IL-1β，TNF-ɑ及 hs-CRP水平，同时检测 IL-1β基因 rs2853550 A/G多态性。结果　GA组 IL-1β，TNF-ɑ及 hs-CRP水平分别为 5.16±1.02pg/ml，21.84±6.49pg/ml和 17.03±5.61mg/L，明显高于对照组（ 3.94±0.87pg/ml，3.50±1.16pg/ml及 2.14±0.73mg/L），差异均有统计学意义（ t=4.217 3，20.510 4，23.274 5，均 P<0.05）。GA组 IL-1β基因 rs2853550 A/G位点 GG基因型和 G等位基因频率（ 75.93%和 83.56%）明显高于对照组（ 51.63%和 60.13%），差异有统计学意义（ χ2=5.641 9，5.037 2，均 P<0.05）。GG基因型和 G等位基因患 GA的相对风险明显增加（ OR=7.092，95%CI：5.681~10.526和 OR=5.914，95％ CI：3.514～ 8.029），而 AA基因型和 A等位基因患 GA的相对风险明显降低（ OR=0.731，95%CI：0.576～ 0.918和 OR=0.584，95％ CI：0.437~0.753）。GA组 GG基因型 IL-1β水平（5.42±1.12pg/ml）明显高于 GA基因型（ 4.51±0.67pg/ml），而 GA基因型明显高于 AA基因型（ 4.04±0.29pg/ml），不同基因型之间差异有统计学意义（ F=8.019 5，P=0.023 6）。经 Spearman相关性分析， GA组 IL-1β与 TNF-ɑ，hs-CRP水平呈正相关（ r=0.790 1，0.684 7，均 P<0.05）。结论　GA患者 IL-1β，TNF-ɑ及 hs-CRP水平明显升高，且存在一定相关性。同时 IL-1β基因启动子区 rs2853550 A/G位点呈多态性，其中 GG基因型可能与 GA发病具有一定的相关性。
Abstract:
Objective　To analyze the levels of interleukin-1β(IL-1β),Tumor necrosis factor(TNF-ɑ),hypersensitive C-reactiveprotein (hs-CRP) and its association with polymorphism of IL-1βgene promoter region rs2853550 A/G loci in patients withgouty arthritis. Methods　216 patients diagnosed with GA were collected as the GA group, and 153 healthy subjects wereselected as control group. The levels of IL-1β,TNF-ɑand hs-CRP in serum were detected, and the polymorphism of IL-1βgene rs2853550 A/G was analyzed. Results　The levels of IL-1β,TNF-ɑand hs-CRP in GA group were 5.16±1.02pg/ml，21.84±6.49pg/ml and 17.03±5.61mg/L, respectively, which were significantly higher than those in control group(3.94±0.87pg/ml，3.50±1.16pg/ml and 2.14±0.73mg/L),the difference were statistically significant(t=4.217 3，20.510 4，23.274 5，allP <0.05).The frequencies of GG genotype and G allele of IL-1β rs2853550 A/G loci in GA group(75.93% and 83.56%) weresignificantly higher than those in control group(51.63% and 60.13%),the difference was statistically significant(χ2=5.641 9, 5.0372, all P<0.05).The relative risk of GA was significantly increased for GG genotype and G allele(OR=7.092,95%CI:5.681~10.526and OR=5.914,95%CI:3.514~8.029),while the relative risk of GA was significantly decreased for AA genotype and A allele(OR=0.731,95%CI:0.576 ～ 0.918 and OR=0.584,95%CI:0.437~ 0.753).The level of IL-1β in GG genotype (5.42±1.12pg/ml) was significantly higher than that in GA genotype (4.51±0.67pg/ml), while that in GA genotype was significantly higherthan that in AA genotype (4.04±0.29pg/ml),the differences among different genotypes were statistically significant(F=8.019 5,P = 0.023 6).Spearman correlation analysis showed that IL-1β was positively correlated with TNF-ɑand hs-CRP levels in GAgroup(r=0.790 1, 0.684 7,all P<0.05). Conclusion　The levels of IL-1β, TNF-ɑand hs-CRP were significantly increased in GApatients, and there was a certain correlation. At the same time, the rs2853550 A/G site in the promoter region of IL-1βgene waspolymorphic, and GG genotype may have a certain correlation with the incidence of GA.

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备注/Memo

备注/Memo:
作者简介：叶科导（1980-），男，学士，主治医师，主要从事临床内科疾病的诊断、治疗及预防工作，E-mail：Ykd666888@163.com。
更新日期/Last Update: 1900-01-01