[1]王淑明a,徐建男a,张建新b,等.维持性血液透析患者血清Beclin1和LC3-II表达水平与血管钙化的相关性分析[J].现代检验医学杂志,2022,37(02):132-136.[doi:10.3969/j.issn.1671-7414.2022.02.027]
　WANG Shu-minga,XU Jian-nana,ZHANG Jian-xinb,et al.Correlation between Serum Beclin1 and LC3-II Expression Levels and Vascular Calcification in Maintenance Hemodialysis Patients[J].Journal of Modern Laboratory Medicine,2022,37(02):132-136.[doi:10.3969/j.issn.1671-7414.2022.02.027]
点击复制

维持性血液透析患者血清Beclin1和LC3-II表达水平与血管钙化的相关性分析()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第37卷
期数:
2022年02期
页码:
132-136
栏目:
论　著
出版日期:
2022-03-15

文章信息/Info

Title:
Correlation between Serum Beclin1 and LC3-II Expression Levels and Vascular Calcification in Maintenance Hemodialysis Patients
文章编号:
1671-7414（2022）02-132-06
作者:
王淑明a; 徐建男a; 张建新b; 王月华a; 康京月a; 刘 畅a
（三河燕郊福合第一医院/ 原三河市燕郊人民医院a. 肾脏病内科；b. 急诊科，河北三河 065201）
Author(s):
WANG Shu-minga;  XU Jian-nana;  ZHANG Jian-xinb;  WANG Yue-huaa;  KANG Jing-yuea;  LIU Changa
(a.Department of Nephrology; b.Department of Emergency, the Yanjiao Fuhe First Hospital of Sanhe City/Yanjiao People’s Hospital of Sanhe City,Hebei Sanhe 065201,China)
关键词:
维持性血液透析; Beclin1; 微管相关蛋白1轻链3-II; 血管钙化
分类号:
R459.5；R392.11
DOI:
10.3969/j.issn.1671-7414.2022.02.027
文献标志码:
A
摘要:
目的　探究维持性血液透析（maintenance hemodialysis，MHD）患者血清Beclin1 和微管相关蛋白1 轻链3-II（microtubule associated protein 1 light chain 3-II，LC3-II）表达水平与血管钙化（vascular calcification，VC）的相关性。方法　选取2017 年4 月～ 2020 年9 月三河市燕郊人民医院收治的MHD 患者145 例作为研究对象，根据是否发生VC，将患者分为MHD 未并发VC 组（n=75）和MHD 并发VC 组（n=70）。根据VC 评估结果将MHD 并发VC 患者分为轻度VC 组（n=36）、中度VC 组（n=24）、重度VC 组（n=10）；另选取同期在该院进行体检的健康人80 例作为对照组。收集受试者一般资料，酶联免疫吸附法（enzyme-linked immunosorbent assay，ELISA）检测受试者血清Beclin1 和LC3-II 的水平，Pearson 法分析MHD 患者血清Beclin1 和LC3-II 水平与VC 相关指标的相关性，二元logistic回归分析MHD 患者发生VC 的影响因素。结果　与对照组相比，MHD 未并发VC 组和MHD 并发VC 组血清肌酐（serum creatinine，SCr）（72.48±18.26μmol/L vs 685.30±192.42μmol/L ，733.98±206.35μmol/L）、血磷（1.28±0.42mmol/L vs 2.08±0.71mmol/L。2.86±0.87mmol/L）、血钙（1.54±0.45mmol/L vs 2.46±0.62mmol/L ，2.98±0.77mmol/L）、全段甲状旁腺激素（intact parathyroid hormone，iPTH）（58.10±17.36pg/ml vs 634.58±172.44pg/ml，769.48±195.02pg/ml）水平升高，差异有统计学意义（F=100.197 ～ 500.960，均P ＜ 0.05）；血清血红蛋白（hemoglobin，Hb）（134.82±35.16g/L vs 112.78±32.85g/L，103.66±27.91g/L），Beclin1（8.09±2.16μg/L vs 5.65±1.43μg/L，2.56±0.73μg/L）和LC3-II（45.16±5.15μg/L vs 36.31±3.42μg/L ，27.47±2.76μg/L）水平降低，差异有统计学意义（F=18.748 ～ 372.522，均P ＜ 0.05）；与MHD 未并发VC 组相比，MHD 并发VC 组血磷、血钙及iPTH 水平升高（t=7.136 ～ 9.723，均P＜ 0.05），血清Beclin1 和LC3-II 水平降低（t=16.605，18.982，均P ＜ 0.05）。轻度VC 组、中度VC 组和 重度VC组血清Beclin1（4.35±0.71μg/L，3.49±0.57μg/L 和1.91±0.26μg/L）和LC3-II（31.12±3.32μg/L，25.65±2.62μg/L vs 20.47±1.76μg/L）水平依次降低，差异均有统计学意义（F=366.298，296.025，均P ＜ 0.05）。MHD 患者血清Beclin1 水平与血磷、血钙及iPTH 均呈负相关（r=-0.674，-0.682，-0.597，均P ＜ 0.05）；血清LC3-II 水平与血磷、血钙及iPTH 也呈负相关（r=-0.648，-0.703，-0.674，均P ＜ 0.05）。二元logistic 回归分析发现，Beclin1 和LC3-II水平偏低是MHD 患者发生VC 的危险因素（P ＜ 0.05）。结论　MHD 并发VC 患者血清Beclin1 和LC3-II 水平降低，与VC 严重程度有关，是MHD 患者发生VC 的危险因素，可作为预测MHD 患者发生VC 的潜在生物学标志物。
Abstract:
Objective　To explore the correlation between the expression levels of serum Beclin1 and microtubule associated protein 1 light chain 3-II (LC3-II) and vascular calcification (VC) in maintenance hemodialysis (MHD) patients. Methods　A total of 145 MHD patients admitted to Yanjiao People’s Hospital of Sanhe City from April 2017 to September 2020 were selected as the research objects. According to the occurrence of VC, the patients were divided into MHD without VC group (n=75) and MHD with VC group (n=70). According to the results of VC evaluation, MHD patients with VC were divided into mild VC group (n=36), moderate VC group (n=24) and severe VC group (n=10). Another 80 healthy people were selected as thecontrol group.The general data of the subjects were collected, and the levels of serum Beclin1 and LC3-II were detected by enzyme-linked immunosorbent assay (ELISA). The correlation between serum Beclin1 and LC3-II levels and VC related indicators in MHD patients was analyzed by Pearson method. The influencing factors of VC in MHD patients were analyzed by binary logistic regression. Results　Compared with those in the control group, the levels of serum creatinine (SCr) (72.48±18.26μmol/L vs 685.30±192.42μmol/L, 733.98±206.35μmol/L), serum phosphorus (1.28±0.42mmol/L vs 2.08±0.71mmol/L ， 2.86±0.87mmol/L), calcium (1.54±0.45mmol/L vs 2.46±0.62mmol/L ，2.98±0.77mmol/L), intact parathyroid hormone (iPTH) (58.10±17.36pg/ml vs 634.58±172.44pg/ml ，769.48±195.02pg/ml) in MHD without VC group and MHD with VC group increased，the differences were statistically significant(F=100.197 ～ 500.960,all P ＜ 0.05), and the levels of hemoglobin (Hb) (134.82±35.16g/L vs 112.78±32.85g/L, 103.66±27.91g/L), Beclin1 (8.09±2.16μg/L vs 5.65±1.43μg/L, 2.56±0.73μg/L) and LC3-II (45.16±5.15μg/L vs 36.31±3.42μg/L, 27.47±2.76μg/L) in serum were decreased，the differences were statistically significant (F=18.748 ～ 372.522, all P ＜ 0.05). Compared with those in MHD without VC group, the levels of serum phosphorus, calcium and iPTH in MHD with VC group were higher (t=7.136 ～ 9.723, all P ＜ 0.05), and the levels of serum Beclin1 and LC3- II were lower (t=16.605, 18.982, all P ＜ 0.05). The levels of Beclin1 (4.35±0.71μg/L vs 3.49±0.57μg/L, 1.91±0.26μg/L) and LC3-II (31.12±3.32μg/L vs 25.65±2.62μg/L，20.47±1.76μg/L) in mild VC group, moderate VC group and severe VC group decreased in turn，the differences were statistically significant (F=366.298, 296.025，all P ＜ 0.05). The serum Beclin1 level was negatively correlated with serum phosphorus, calcium and iPTH in MHD patients (r=-0.674, -0.682，-0.597, all P ＜ 0.05). The level of serum LC3-II was negatively correlated with serum phosphorus, calcium and iPTH (r=-0.648, -0.703，-0.674, all P ＜ 0.05). Binary logistic regression analysis showed that low levels of Beclin1 and LC3-II were risk factors for VC in MHD patients (P ＜ 0.05). Conclusion　The levels of Beclin1 and LC3-II in MHD patients with VC were decreased, they were related to the severity of VC, which are risk factors for the occurrence of VC in MHD patients, and may be potential biomarker for predicting VC in MHD patients.

参考文献/References:

[1] 冯琴, 尚小玲, 李智, 等. 慢性肾脏疾病晚期患者的尿代谢组学特征分析[J]. 现代检验医学杂志, 2019, 34(1):93-96. FENG Qin, SHANG Xiaoling, LI Zhi, et al. Feature analysis of urinary matabolism in advanced chronic kidney disease [J]. Journal of Modern Laboratory Medicine, 2019, 34(1):93-96.
[2] 杨艳艳, 韩锦, 桂保松, 等.CaSR/ 自噬信号轴介导 枸橼酸二乙酯抑制慢性肾脏病血管钙化[J]. 西安交 通大学学报（医学版）, 2020, 41(5):701-705. YANG Yanyan, HAN Jin, GUI Baosong, et al. Diethyl citrate inhibits vascular calcification in chronic kidney disease by CaSR/autophagy signaling axis [J]. Journal of Xi’an Jiaotong University(Medical Sciences), 2020, 41(5):701-705.
[3] QIN Xiang, LU Aimei, KE Meilin, et al. DJ-1 inhibits autophagy activity of prostate cancer cells by repressing JNK-Bcl2-Beclin1 signaling[J]. Cell Biology International, 2020, 44(4): 937-946.
[4] 彭娜, 胡泽昆, 胡大军. 自噬相关分子Beclin1 和 LC3在糖尿病肾脏疾病患者血清中的表达及意义[J]. 临床肾脏病杂志, 2018, 18(7):417-420. PENG Na, HU Zekun, HU Dajun. Expression of Beclin1 and LC3 in diabetic kidney disease and significance [J]. Journal of Clinical Nephrology, 2018, 18(7):417-420.
[5] 张欣, 王瑞元. 大负荷运动诱导大鼠骨骼肌损伤对 其自噬超微结构及Beclin1和LC3- Ⅱ / Ⅰ的影响[J]. 中国应用生理学杂志, 2020, 36(4):296-300, 后插1. ZHANG Xin, WANG Ruiyuan. Effects of high-load exercise induced skeletal muscle injury on autophagy ultrastructure and Beclin1 and LC3- Ⅱ / Ⅰ in rats [J]. Chinese Journal of Applied Physiology, 2020, 36(4):296-300, after insert 3.
[6] 石现军, 武黎平, 王兆成, 等．维持性血液透析患者 血清miR-26 a, IGF-1 表达与血管钙化的关系[J]． 中国中西医结合肾病杂志, 2019, 20(10):888-890. SHI Xianjun, WU Liping, WANG Zhaocheng, et al. Relationship between serum miR-26 a, IGF-1 expression and vascular calcification in maintenance hemodialysis patients[J]. Chinese Journal of Integrated Traditional and Western Nephrology, 2019, 20(10):888- 890.
[7] XIONG Jiachuan, HE Ting, WANG Min, et al. Serum magnesium, mortality, and cardiovascular disease in chronic kidney disease and end-stage renal disease patients: a systematic review and meta-analysis[J]. Journal of Nephrology, 2019, 32(5): 791-802.
[8] CHOI S R, LEE Y K, CHO A J, et al. Malnutrition, inflammation, progression of vascular calcification and survival: Inter-relationships in hemodialysis patients[J]. PLoS One, 2019, 14(5): e0216415.
[9] LEE C T, LEE Y T, TAIN Y L, et al. Circulating microRNAs and vascular calcification in hemodialysis patients[J]. The Journal of International Medical Research, 2019, 47(7): 2929-2939.
[10] HENZE L A, LUONG T T D, BOEHME B, et al. Impact of C-reactive protein on osteo-/chondrogenic transdifferentiation and calcification of vascular smooth muscle cells[J]. Aging, 2019, 11(15): 5445-5462.
[11] LEE S J, LEE I K, JEON J H. Vascular calcificationnew insights into its mechanism[J]. International Journal of Molecular Sciences, 2020, 21(8): 2685.
[12] NAPOLETANO F, BARON O, VANDENABEELE P, et al. Intersections between regulated cell death and autophagy[J]. Trends in Cell Biology, 2019, 29(4): 323- 338.
[13] LEE H Y, OH S H. Autophagy-mediated cytoplasmic accumulation of p53 leads to apoptosis through DRAM-BAX in cadmium-exposed human proximal tubular cells[J]. Biochemical and Biophysical Research Communications, 2021, 534(1): 128-133.
[14] HE Huqiang, LAW B Y K, ZHANG Ni, et al. Bavachin protects human aortic smooth muscle cells against β-glycerophosphate-mediated vascular calcification and apoptosis via activation of mTOR-dependent autophagy and suppression of β-catenin signaling [J]. Frontiers in Pharmacology, 2019, 10(7): 1427.
[15] 邱小波, 盛子桐, 韩怡然, 等．虫草菌液通过增强自 噬改善β- 甘油磷酸诱导的血管平滑肌细胞钙化[J]. 中华肾脏病杂志, 2019, 35(2):119-126. QIU Xiaobo, SHENG Zitong, HAN Yiran, et al. Cordyceps sinensis alleviates β-glycerophosphateinduced vascular smooth muscle call calcification through promoting autophagy [J]. Chinese Journal of Nephrology, 2019, 35(2):119-126.
[16] WONG M, GANAPATHY A S, SUCHANEC E, et al. Intestinal epithelial tight junction barrier regulation by autophagy-related protein ATG6/beclin 1[J]. American Journal of Physiology-Cell Physiology, 2019, 316(5): C753-C765.
[17] ARAI A, KIM S, GOLDSHTEYN V, et al. Beclin1 modulates bone homeostasis by regulating osteoclast and chondrocyte differentiation[J]. Journal of Bone and Mineral Research 2019, 34(9): 1753-1766.
[18] BANSAL M, MOHARIR S C, SWARUP G. Autophagy receptor optineurin promotes autophagosome formation by potentiating LC3-II production and phagophore maturation[J]. Communicative & Integrative Biology, 2018, 11(2): 1-4.
[19] CHEN Weiren, YANG Jiaqi, LIU Fang, et al. Melatonin attenuates vascular calcification by activating autophagy via an AMPK/mTOR/ULK1 signaling pathway[J]. Experimental Cell Research, 2020, 389(1): 111883.
[20] 刘成, 许雅倩, 邹琪．脓毒症患者外周血淋巴细胞 自噬相关基因Beclin-1 和LC3 的变化研究[J]．中 华全科医学, 2020, 18(6):913-916. LIU Cheng, XU Yaqian, ZOU Qi. Study on the changes of Beclin-1 and LC3 related to autophagy in peripheral blood lymphocytes of patients with sepsis [J]. Chinese Journal of General Practice, 2020, 18(6):913-916.
[21] FRAUSCHER B, KIRSCH A H, SCHABH?TTL C, et al. Autophagy protects from uremic vascular media calcification[J]. Frontiers in Immunology, 2018, 9(6): 1866.

相似文献/References:

[1]封建华,叶建明,赵 毅,等.终末期肾病维持性血液透析患者血清SORT 和IGF-1 水平表达与心血管疾病发生及预后的相关性研究[J].现代检验医学杂志,2024,39(03):125.[doi:10.3969/j.issn.1671-7414.2024.03.021]
　FENG Jianhua,YE Jianming,ZHAO Yi,et al.Correlation among the Expression of Serum SORT and IGF-1 Levels in Maintenance Hemodialysis Patients with End Stage Renal Disease and the Occurrence and Prognosis of Cardiovascular Diseases[J].Journal of Modern Laboratory Medicine,2024,39(02):125.[doi:10.3969/j.issn.1671-7414.2024.03.021]
[2]苏玉萍,王燕梅.维持性血液透析患者血液Lp(a)，Fib水平检测与自体动静脉内瘘血管钙化及严重程度的关系[J].现代检验医学杂志,2024,39(06):141.[doi:10.3969/j.issn.1671-7414.2024.06.024]
　SU Yuping,WANG Yanmei.Relationship between Lp (a), Fib Levels and Vascular Calcification and Severity of Autogenous Arteriovenous Fistula in Maintenance Hemodialysis Patients[J].Journal of Modern Laboratory Medicine,2024,39(02):141.[doi:10.3969/j.issn.1671-7414.2024.06.024]

备注/Memo

备注/Memo:
基金项目：河北省医学科学研究课题计划项目（编号20200115）；廊坊市科学技术研究与发展计划项目（编号2018013135）。
作者简介：王淑明（1979-），女，本科，主治医师，研究方向：维持性血液透析患者并发症的治疗，E-mail：shumingwang795@163.com。
更新日期/Last Update: 1900-01-01