[1]王　景,董　坤,黄　优,等.急性缺血性脑卒中患者血清miR-433-5p表达水平及其与Hcy及MMP-9的相关性研究[J].现代检验医学杂志,2022,37(03):87-90.[doi:10.3969/j.issn.1671-7414.2022.03.018]
　WANG Jing,DONG Kun,HUANG You,et al.Expression of Serum miR-433-5p and Its Correlation with Hcy and MMP-9 in Patients with Acute Ischemic Stroke[J].Journal of Modern Laboratory Medicine,2022,37(03):87-90.[doi:10.3969/j.issn.1671-7414.2022.03.018]
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急性缺血性脑卒中患者血清miR-433-5p表达水平及其与Hcy及MMP-9的相关性研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第37卷
期数:
2022年03期
页码:
87-90
栏目:
论　著
出版日期:
2022-05-15

文章信息/Info

Title:
Expression of Serum miR-433-5p and Its Correlation with Hcy and MMP-9 in Patients with Acute Ischemic Stroke
文章编号:
1671-7414（2022）03-087-04
作者:
王　景; 董　坤; 黄　优; 陈海恋; 林兆信; 何超明
（三亚中心医院/ 海南省第三人民医院神经内科，海南三亚 572000）
Author(s):
WANG Jing;  DONG Kun;  HUANG You;  CHEN Hai-lian;  LIN Zhao-xin;  HE Chao-ming
（Department of Neurology，Sanya Central Hospital/ the Third People’s Hospital of Hainan Province,Hainan Sanya 572000,China）
关键词:
急性缺血性脑卒; 微小核糖核酸-433-5p; 同型半胱氨酸; 基质金属蛋白酶-9
分类号:
R743.3；R392.11
DOI:
10.3969/j.issn.1671-7414.2022.03.018
文献标志码:
A
摘要:
目的　探讨急性缺血性脑卒中（ acute ischemic stroke，AIS）患者血清微小核糖核酸（ micro RNA, miR）-4335p表达与同型半胱氨酸（ homocysteine ，Hcy）及基质金属蛋白酶 9（matrix metalloproteinase-9，MMP-9）的相关性，为 AIS的诊疗提供依据。方法　选取 2019年 1月～ 2021年 3月三亚中心医院收治的 106例 AIS，采用美国国立卫生研究院卒中量表（ NIHSS）评分分为轻度组（ n=30，NIHSS评分＜ 5分）、中度组（ n=48，5分≤ NIHSS评分≤ 20分）、重度组（n=28，national institute of health stroke scale, NIHSS评分＞ 20分）。另选择 60例健康体检正常者作为对照组。采用实时荧光定量 PCR检测各组血清 miR-433-5p表达水平。应用 ROC曲线分析血清 miR-433-5p ，Hcy及 MMP-9水平对 AIS诊断的价值， Pearson相关分析 AIS患者血清 miR-433-5p表达水平与 Hcy及 MMP-9的相关性。结果　AIS组血清 miR-433-5p（2.96±0.94 ），Hcy（19.14±5.30μmol/L）及 MMP-9（151.36±29.75μg/L）水平高于对照组（0.81±0.17， 5.65±1.13μmol/L，77.93±11.28μg/L），差异均有统计学意义（ t=16.718,14.216,10.152, 均 P＜ 0.001）。重度组血清 miR-433-5p（4.36±1.20 ），Hcy（29.12±6.48μmol/L）及 MMP-9（227.15±48.20μg/L）水平均高于中度组（ 2.80±0.91，18.20±4.86μmol/L，140.83±25.64μg/L）和轻度组（ 1.83±0.57，11.35±3.24μmol/L，85.27±11.93μg/L），差异均有统计学意义（ t=9.847～ 14.636，均 P＜ 0.001）。ROC曲线显示，miR-433-5p诊断 AIS的曲线下面积（ AUC）最大（ 0.851， 95%CI：0.792～ 0.908），其敏感度和特异度分别为 89.4%，82.0%。相关分析显示， AIS患者血清 miR-433-5p表达水平与 Hcy及 MMP-9均呈正相关（ r=0.842，0.714，P＜ 0.01）。结论　血清 miR-433-5p表达水平在 AIS患者中明显升高，且与 Hcy及 MMP-9水平相关，有望作为 AIS诊断的有用标志物。
Abstract:
Objective　To investigate the correlation between serum miR-433-5p expression and homocysteine (Hcy) and matrix metalloproteinase-9 (MMP-9) in patients with acute ischemic stroke (AIS), so as to provide basis for the diagnosis and treatment of AIS. Methods　106 cases of AIS admitted to Sanya Central Hospital from January 2019 to March 2021 were selected. The national institutes of health stroke scale (NIHSS) score were used to divide the patients into mild group (n=30, NIHSS score< 5), moderate group (n=48, 5 ≤ NIHSS score ≤ 20), and severe group (n=28, NIHSS score >20). Another 60 healthy subjects were selected as control group. Real-time fluorescent quantitative PCR was used to detect the expression of serum miR-433-5p in each group. The diagnostic value of serum levels of miR- 433-5p, Hcy and MMP-9 in AIS patients were analyzed by ROC curve. Pearson correlation analysis of serum levels of miR-433-5p and their correlation with Hcy and MMP-9 in AIS patients. Results　 The levels of serum miR-433-5p（2.96±0.94）, Hcy（19.14±5.30μmol/L） and MMP-9（151.36±29.75μg/L） in AIS group were higher than those in control group（0.81±0.17, 5.65±1.13μmol/L, 77.93±11.28μg/L）, the differences were statistically significant（t=16.718, 14.216, 10.152, all P ＜ 0.001）. The levels of serum miR-433-5p（4.36±1.20）, Hcy （29.12±6.48μmol/L） and MMP-9（227.15±48.20μg/L）in severe group were higher than those in moderate group （2.80±0.91，18.20±4.86μmol/L，140.83±25.64μg/L）and mild group（1.83±0.57，11.35±3.24μmol/L， 85.27±11.93μg/L）, the differences were statistically significant（t=9.847 ～ 14.636，all P ＜ 0.001）. ROC curve showed that the area under the curve (AUC) of miR- 433- 5p in the diagnosis of AIS was the largest (0.851, 95% CI: 0.792 ～ 0.908), with a sensitivity of 89.4% and specificity of 82.0%, respectively. Relevant analysis showed that levels of serum miR -433-5p were positively correlated with Hcy and MMP-9 in patients with AIS（r=0.842，0.714，P ＜ 0.01）.Conclusion　Serum miR- 433- 5p expression level was significantly increased in AIS patients, and were correlated with Hcy and MMP-9 levels, which is expected to be a useful marker for AIS diagnosis.

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备注/Memo

备注/Memo:
基金项目：海南省医药卫生科研基金项目(18A200005)。
作者简介：王景（1981-），本科，女，副主任医师，主要从事临床神经内科疾病研究,E-mail：a58398895a@163.com。
更新日期/Last Update: 1900-01-01