[1]王海峰,张　越,舒　放,等.呼吸道合胞病毒感染患儿外周血NDR1和miR-146a表达水平及其临床意义[J].现代检验医学杂志,2022,37(03):110-113.[doi:10.3969/j.issn.1671-7414.2022.03.023]
　WANG Hai-feng,ZHANG Yue,SHU Fang,et al.Expression Levels of NDR1 and miR-146a in Peripheral Blood of Children with Respiratory Syncytial Virus Infection and Their Clinical Significance[J].Journal of Modern Laboratory Medicine,2022,37(03):110-113.[doi:10.3969/j.issn.1671-7414.2022.03.023]
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呼吸道合胞病毒感染患儿外周血NDR1和miR-146a表达水平及其临床意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第37卷
期数:
2022年03期
页码:
110-113
栏目:
论　著
出版日期:
2022-05-15

文章信息/Info

Title:
Expression Levels of NDR1 and miR-146a in Peripheral Blood of Children with Respiratory Syncytial Virus Infection and Their Clinical Significance
文章编号:
1671-7414（2022）03-011-05
作者:
王海峰1; 张　越2; 舒　放1; 白　云1; 薛飞肖1; 史珊珊1; 孙大庆1
（1. 西北大学附属医院/ 西安市第三医院检验科，西安 710082；2. 西安市儿童医院中心实验室，西安 710002）
Author(s):
WANG Hai-feng1; ZHANG Yue2; SHU Fang1; BAI Yun1; XUE Fei-xiao1; SHI Shan-shan1; SUN Da-qing1
（1.Department of Clinical Laboratory, the Affiliated Hospital of Northwest University/Xi’an No.3 Hospital，Xi’an 710082，China；2.Central Laboratory，Xi’an Children’s Hospital，Xi’an 710002，China）
关键词:
呼吸道合胞病毒感染; 丝氨酸/ 苏氨酸激酶38; 微小核糖核酸-146a
分类号:
R373.14；R392.11
DOI:
10.3969/j.issn.1671-7414.2022.03.023
文献标志码:
A
摘要:
目　的　探讨丝苏氨酸蛋白激酶 1(Nuclear Dbf2 p-related kinase 1, NDR1)和微小核糖核酸（micro RNA, miR）146a在呼吸道合胞病毒（ Respiratory syncytial virus，RSV）感染患儿外周血清中的表达情况及临床意义。方法　收集 2019年 7月～ 2020年 5月西北大学附属医院（西安市第三医院）检验科、西安市儿童医院就诊的 77例 RSV感染患儿作为研究对象。按照病情严重程度分为重症组 36例，轻症组 41例。同时选取 53例同期来医院体检的健康儿童为对照组。采用实时荧光定量 PCR（qRT-PCR）法检测外周血清中 miR-146a表达水平；酶联免疫吸附法（ ELISA）检测 NDR1水平；Pearson法分析 NDR1与 miR-146a表达的相关性及二者与相关炎性因子表达的相关性。受试者工作特征曲线（ ROC）评估血清 NDR1和 miR-146a水平对 RSV感染严重程度的诊断价值。结果　与对照组相比， RSV感染患儿外周血中 NDR1水平（ 9.95±2.38 pg/L vs 6.46±1.12 pg/L）下调， miR-146a表达水平（ 1.03±0.17 vs 2.12±0.43）上调，差异具有统计学意义（ tmiR-146a=17.519，tNDR1=11.204，均 P＜ 0.05）。RSV感染重症组、轻症组及对照组中 hs-CRP，IL-4， IL-5，IL-6，IL-2和 IFN-γ比较差异均有统计学意义（ F=67.825，564.775，256.06，202.992，77.579，320.470，均 P＜ 0.05）。 RSV感染患儿血清 NDR1水平与 IL-2，IFN-γ表达均呈正相关（ r=0.473，0.620，均 P＜ 0.05），与 hs-CRP，IL-4， IL-5，IL-6水平呈负相关（ r=-0.259，-0.559，-0.422，-0.505，均 P＜ 0.05）； miR-146a与 hs-CRP，IL-4，IL-5，IL-6水平均呈正相关（ r=0.381，0.636，0.421，0.570，均 P＜ 0.05），与 IL-2，IFN-γ水平呈负相关（ r=-0.646，-0.744，均 P＜ 0.05）；RSV感染患儿外周血清中 NDR1与 miR-146a表达水平呈负相关（ r=-0.225，P＜ 0.05）；ROC结果显示，血清 NDR1，miR-146a水平预测重症 RSV感染患儿的曲线下面积（ AUC）分别为 0.911（95%CI：0.823～ 0.964）， 0.741（95%CI：0.628～ 0.834），敏感度分别为 82.93%，68.29%，特异度分别为 88.89%，75.00%，二者联合预测重症 RSV感染患儿的 AUC为 0.925（95%CI：0.842～ 0.973），敏感度和特异度分别为 90.24%和 83.33%。结论　NDR1在 RSV感染患儿外周血清中呈低表达，miR-146a呈高表达，二者可作为评估患儿病情发展的潜在指标。
Abstract:
Objective　To investigate the expression and clinical significance of Nuclear Dbf2p-related kinase 1 ( NDR1) and micro RNA（miR）-146a in the peripheral serum of children infected with Respiratory syncytial virus (RSV). Methods　Blood samples of children with RSV infection in the Affiliated Hospital of Northwest University/Xi’an No.3 Hospital，Xi’an Children’s Hospital from July 2019 to May 2020 were collected. According to the severity of the disease, there were 36 cases in the severe group and 41 cases in the mild group. At the same time, 53 healthy children who came to the hospital for physical examination during the same period were selected as the control group.The expression level of miR-146a in peripheral blood was detected by real-time fluorescence quantitative PCR (qRT-PCR). NDR1 was detected by enzyme-linked immunosorbent assay (ELISA). The correlation between the expression of NDR1 and miR-146a and the expression of related inflammatory factors was analyzed by Pearson method. Receiver operating characteristic curve (ROC) was used to assess the diagnostic value of serum NDR1 and miR-146a levels for the severity of RSV infection. Results　Compared with the control group, the level of NDR1 (9.95±2.38 pg/L vs 6.46±1.12 pg/L) in peripheral blood of children infected with RSV was significantly down-regulated, and the expression level of miR-146a (1.03±0.17 vs 2.12±0.43) was up-regulated. All were statistically significant (tmiR-146a=17.519， tNDR1=11.204，all P ＜ 0.05). There were significant differences in hs-CRP, IL-4, IL-5, IL-6, IL-2 and IFN-γ in RSV infection severe group, mild group and control group (F=67.825, 564.775, 256.06, 202.992, 77.579, 320.470, all P ＜ 0.05). The serum NDR1 level in children with RSV infection was positively correlated with IL-2 and IFN-γ expression (r=0.473, 0.620, all P ＜ 0.05), and negatively correlated with hs-CRP, IL-4, IL-5 and IL-6 levels (r=-0.259, -0.559, -0.422, -0.505，all P ＜ 0.05); MiR-146a was positively correlated with hs-CRP, IL-4, IL-5 and IL-6 levels (r=0.381, 0.636, 0.421, 0.570，all P ＜ 0.05), and negatively correlated with IL-2 and IFN-γ levels (r=-0.646, -0.744, all P ＜ 0.05). The expression levels of NDR1 and miR- 146a in the peripheral serum of children infected with RSV were negatively correlated (r=-0.225, P ＜ 0.05). ROC results showed that the area under the curve (AUC) of serum NDR1 and miR-146a levels predicting children with severe RSV infection was 0.911 (95%CI: 0.823 ～ 0.964) and 0.741 (95%CI: 0.628 ～ 0.834), the sensitivity was 82.93% and 68.29%, and the specificity was 88.89% and 75.00%, respectively. The AUC of the combination of the two predicting children with severe RSV infection was 0.925 (95%CI: 0.842 ～ 0.973), and the sensitivity and the specificity were 90.24%, 83.33%，respectively. Conclusion　The low expression of NDR1 and high expression of miR-146a in peripheral blood of children with RSV infection can be used as potential indicators to evaluate the disease progression of children..

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备注/Memo

备注/Memo:
基金项目：陕西省科学技术厅重点研发计划项目（2021SF-036）。
作者简介：王海峰（1971-），男，主任技师，主要研究方向：临床检验管理与诊断，E-mail：xasyjykwhf@sina.com。
更新日期/Last Update: 1900-01-01