[1]罗　钢,蔡丽英,呙　明.视网膜母细胞瘤组织中miR-142的表达及对细胞增殖和侵袭力的影响[J].现代检验医学杂志,2022,37(03):144-148.[doi:10.3969/j.issn.1671-7414.2022.03.030]
　LUO Gang,CAI Li-ying,GUO Ming.Expression of miR-142 in Retinoblastoma Tissues and Influences on Cell Proliferation and Invasiveness[J].Journal of Modern Laboratory Medicine,2022,37(03):144-148.[doi:10.3969/j.issn.1671-7414.2022.03.030]
点击复制

视网膜母细胞瘤组织中miR-142的表达及对细胞增殖和侵袭力的影响()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第37卷
期数:
2022年03期
页码:
144-148
栏目:
研究简报·实验技术
出版日期:
2022-05-15

文章信息/Info

Title:
Expression of miR-142 in Retinoblastoma Tissues and Influences on Cell Proliferation and Invasiveness
文章编号:
1671-7414（2022）03-144-05
作者:
罗　钢1; 蔡丽英1; 呙　明2
（1. 鄂东医疗集团黄石市中心医院湖北理工学院附属医院，湖北黄石 435000；2. 荆州市中心医院，湖北荆州 434000）
Author(s):
LUO Gang1;  CAI Li-ying1;  GUO Ming2
(1. Huangshi Central Hospital of Edong Medical Group /Affiliated Hospital of Hubei Institute of Technology, Hubei Huangshi 435000, China; 2. Jingzhou Central Hospital, Hubei Jingzhou 434000, China)
关键词:
视网膜母细胞瘤; 微小核糖核酸-142; 细胞增殖; 细胞侵袭
分类号:
R739.72；R730.43
DOI:
10.3969/j.issn.1671-7414.2022.03.030
文献标志码:
A
摘要:
目的　探讨微小核糖核酸（ micro RNA, miR）-142在视网膜母细胞瘤（ retinoblastoma，RB）组织中的表达，以及对细胞增殖和侵袭力的影响。方法　选取 2013年 6月～ 2019年 6月在黄石市中心医院接受眼球摘除手术治疗的 RB患儿 79例（79眼），培养人 RB细胞株 Y79并分为 miR-142模拟物组、阴性对照组和空白组，分别转染 miR-142模拟物、阴性对照序列和不作处理， RT-qPCR检测 RB和癌旁组织、细胞中 miR-142表达，MTT法检测细胞增殖活性， Transwell法检测细胞迁移和侵袭力。结果　 miR-142在 RB组织中表达量（ 0.34±0.12）低于癌旁组织（ 0.99±0.16），差异有统计学意义（ t=29.102，P<0.01）；miR-142表达量在不同分化程度、是否神经浸润和淋巴结转移中差异均有统计学意义（ t=2.991～ 3.495，均 P<0.05）；miR-142模拟物组细胞中 miR-142表达量（ 2.42±0.11）高于阴性对照组和空白组（1.02±0.09，1.01±0.10），差异有统计学意义（ F=398.036，P<0.01）；miR-142模拟物组 24 h，48 h，72 h和 96 h时吸光度 A值、迁移细胞数和侵袭细胞数均低于阴性对照组和空白组（F=5.519, 8.666, 12.926, 21.572, 28.394, 27.982, 均 P<0.05）。结论　RB患者组织中 miR-142表达量降低，且与分化程度、神经浸润和淋巴结转移有关。上调 miR-142表达可抑制 Y79细胞增殖、迁移和侵袭能力。
Abstract:
Objective　To investigate the expression of miR-142 in retinoblastoma tissues, and explore its effect on cell proliferation and invasion. Methods　A total of 79 children (79 eyes) with RB who underwent eyeball enucleation surgery in Huangshi Central Hospital were selected from June 2013 to June 2019. The human RB cell line Y79 was cultured and divided into miR-142 mimic group, negative control group and blank group, respectively transfected with miR-142 mimic, negative control sequence and no treatment. The expressions of miR-142 in RB and adjacent tissues and cells were detected by using RT-qPCR. The cell proliferation activity was detected with MTT method.The cell migration and invasion were detected with Transwell method. Results　The expression level of miR-142 in PB tissues was 0.34±0.12, which was lower than 0.99±0.16 in adjacent tissues，the difference was statistically significant (t=29.102, P<0.01). The differences of the expression level of miR-142 between different differentiation, whether nerve infiltration and lymph node metastasis were statistically significantly, the differences were statistically significant (t=2.991 ~ 3.495, all P<0.05). The expression level of miR-142 in the miR-142 mimic group（2.42±0.11） was higher than that in the negative control group and the blank group（1.02±0.09, 1.01±0.10）, the difference was statistically significant(F=398.036, P<0.05). The absorbance A values at 24 h, 48 h, 72 h and 96 h, the numbers of migrating cells and invadingcells in the miR-142 mimic group were lower than those in the negative control group and the blank group (F=5.519, 8.666, 12.926, 21.572, 28.394, 27.982, all P<0.05). Conclusion　The expression level of miR-142 in the RB tissues was reduced, and it was related to the degree of differentiation, nerve infiltration and lymph node metastasis. Up-regulating the expression of miR-142 can inhibit the proliferation, migration and invasion of Y79 cells.

参考文献/References:

[1] 中华医学会眼科学分会眼整形眼眶病学组. 中国单 侧眼内期视网膜母细胞瘤诊疗专家共识(2019 年)[J]. 中华眼科杂志, 2019, 55(4):250-254. Oculoplastic and Orbital Disease Group of Chinese Ophthalmological Society of Chinese Medical Association. Expert consensus on diagnosis and treatment of unilateral intraocular retinoblastoma in China (2019)[J]. Chinese Journal of Ophthalmology, 2019, 55(4):250-254.
[2] HU Huimin, ZHANG Weiling, WANG Yizhuo, et al. Characterization, treatment and prognosis of retinoblastoma with central nervous system metastasis[J]. BMC Ophthalmology, 2018, 18(1): 107.
[3] 林壮玲, 张平. 视网膜母细胞瘤诊疗的研究进展[J]. 眼科学报, 2020, 35(4): 271-278. LIN Zhuangling,ZHANG Ping. Research progress on the diagnosis and treatment of retinoblastoma [J]. Eye Science, 2020, 35(4):271-278.
[4] 管文雪, 彭晓燕. 眼内恶性肿瘤基因靶向治疗研究 进展[J]. 国际眼科纵览, 2020, 44(4): 262-265. GUAN Wenxue, PENG Xiaoyan. Advances in gene targeted therapy for intraocular malignant tumors [J]. International Review of Ophthalmology, 2020, 44(4):262-265.
[5] 郭洁, 池水侠. 急性缺血性脑卒中患者血清miRNA- 181c-5p,miRNA-340-5p 和miRNA146a 水平表达检 测的诊断价值研究[J]. 现代检验医学杂志, 2021, 36(5): 43-45, 89. GUO Jie, CHI Shuixia. Diagnostic value of miRNA- 181c-5p, miRNA-340-5p and miRNA146a levels in patients with acute ischemic stroke [J]. Journal of Modern Laboratory Medicine, 2021, 36(5):43-45,89.
[6] SUN Zhenqiang, SHI Ke, YANG Shuaixi, et al. Effect of exosomal miRNA on cancer biology and clinical applications[J]. Molecular Cancer, 2018, 17(1): 147.
[7] 徐思凡, 张婉婷, 吴官县, 等．miR-142 免疫功能研 究进展[J]. 中国免疫学杂志, 2019, 35(18): 2296-2301. XU Sifan, ZHANG Wanting, WU Guanxian, et al. Research progress of miR-142 in immune function [J]. Chinese Journal of Immunology, 2019, 35(18):2296- 2301
[8] JIN Chang’e, XIAO Liang, ZHOU Zeqiang, et al. MiR-142-3p suppresses the proliferation, migration and invasion through inhibition of NR2F6 in lung adenocarcinoma[J]. Human Cell, 2019, 32(4): 437-446.
[9] DIMARAS H, RETINOBLASTOMA C T. The visible CNS tumor:a review[J]. Journal of Neuroscience Research, 2019, 97(1): 29-44.
[10] 余瑜, 马晓利, 徐艳珍.VEC 方案联合激光治疗RB 眼内期患儿的效果与安全性[J]. 国际眼科杂志, 2019, 19(5):860-862. YU Yu, MA Xiaoli, XU Yanzhen. Efficacy and safety of VEC combined with laser in treatment of children with intraocular RB [J]. International Eye Science, 2019, 19(5):860-862.
[11] 中华医学会眼科学分会眼底病学组, 中华医学会儿 科学分会眼科学组, 中华医学会眼科学分会眼整形 眼眶病学组. 中国视网膜母细胞瘤诊断和治疗指南 (2019 年)[J]. 中华眼科杂志, 2019, 55(10): 726-738. Fundus Ophthalmology Group of Ophthalmology B r a n c h o f C h i n e s e M e d i c a l Ass o c i a t i o n , Ophthalmology Group of Pediatrics Branch of Chinese Medical Association, Ophthalmology and Orbital Diseases Group of Ophthalmology Branch of Chinese Medical Association. Guidelines for the diagnosis and treatment of retinoblastoma in China (2019)[J]. Chin J Ophthalmol, 2019, 55(10):726-738.
[12] 秦静, 石蕊, 张乐, 等．Survivin 基因沉默对视网膜 母细胞瘤凋亡及迁移、侵袭能力的影响[J]. 广西医 科大学学报, 2019, 36(3): 350-354. QIN Jing, SHI Rui, ZHANG Le, et al. Effects of survivin gene silencing on the apoptosis, migration and invasive capability of retinoblastoma HXO-RB cells [J]. Journal of Guangxi Medical University, 2019, 36(3):350-354.
[13] GUAN Luyao, LU Yuan. New developments in molecular targeted therapy of ovarian cancer[J]. Discovery Medicine, 2018, 26(144): 219-229.
[14] TIWARI A, MUKHERJEE B, DIXIT M. MicroRNA key to angiogenesis regulation: miRNA biology and therapy[J]. Current Cancer Drug Targets, 2018, 18(3): 266-277.
[15] LAI W F, LIN M, WONG W T. Tackling aging by using miRNA as a target and a Tool[J]. Trends in Molecular Medicine, 2019, 25(8): 673-684.
[16] 董建新, 张俊梅, 李海城, 等．MiR-142-3p 靶向 HIF1α 对甲状腺癌生物学行为的影响[J]. 医学分子 生物学杂志, 2019, 16(1): 69-75. DONG Jianxin, ZHANG Junmei, LI Haicheng, et al. Effect of miR-142-3p on the biological behaviors of thyroid carcinoma[J]. Journal of Medical Molecular Biology, 2019, 16(1):69-75.
[17] KUNIGENAS L, STANKEVICIUS V, DULSKAS A, et al. 3D cell culture-based global miRNA expression analysis reveals miR-142-5p as a theranostic biomarker of rectal cancer following neoadjuvant Long-Course treatment[J]. Biomolecules, 2020, 10(4): 613.
[18] ZHU Jing, ZHOU Leilei, WEI Bin, et al. MiR-142-5p inhibits pancreatic cancer cell migration and invasion by targeting PIK3CA[J]. Molecular Medicine Reports, 2020, 22(3): 2085-2092.

相似文献/References:

[1]章晋伟,曹刚琴,汪　建.视网膜母细胞瘤组织中赖氨酸羟化酶2表达及其对瘤细胞迁移和侵袭的影响[J].现代检验医学杂志,2022,37(03):105.[doi:10.3969/j.issn.1671-7414.2022.03.022]
　ZHANG Jin-wei,CAO Gang-qin,WANG Jian.Expression of Procollagen-lysine 2-Oxoglutarate 5-Dioxygenase 2 in Retinoblastoma and Its Effect on Tumor Cell Migration and Invasion[J].Journal of Modern Laboratory Medicine,2022,37(03):105.[doi:10.3969/j.issn.1671-7414.2022.03.022]
[2]罗 文,罗 钢,周玉玲,等.视网膜母细胞瘤患儿血清LncRNA-NEAT1 水平表达及对瘤细胞生物学功能的影响[J].现代检验医学杂志,2024,39(03):115.[doi:10.3969/j.issn.1671-7414.2024.03.019]
　LUO Wen,LUO Gang,ZHOU Yuling,et al.Expression of Serum LncRNA NEAT1 in Children with Retinoblastoma and Its Impact on the Biological Function of Tumor Cells[J].Journal of Modern Laboratory Medicine,2024,39(03):115.[doi:10.3969/j.issn.1671-7414.2024.03.019]

备注/Memo

备注/Memo:
基金项目：湖北省卫生厅科研项目（JX6B110）。
作者简介： 罗钢（1975-），男，硕士研究生，副主任医师，研究方向：眼科视神经损伤修复、青光眼、眼外伤、白内障等，E-mail：zhouyuling0099@163.com。
通讯作者：蔡丽英（1977-），女，大学本科，研究方向：眼底病、角膜病眼底病、青光眼、白内障。
更新日期/Last Update: 1900-01-01