[1]王 淼,玄洪雷.老年急性脑梗死患者血清Pannexin1 和sTREM-1 表达水平及其与病情和预后的相关性研究[J].现代检验医学杂志,2022,37(04):123-127.[doi:10.3969/j.issn.1671-7414.2022.04.024]
　WANG Miao,XUAN Hong-lei.Serum Pannexin1 and sTREM-1 Expression Levels in Elderly Patients with Acute Cerebral Infarction and Their Correlation with Disease and Prognosis[J].Journal of Modern Laboratory Medicine,2022,37(04):123-127.[doi:10.3969/j.issn.1671-7414.2022.04.024]
点击复制

老年急性脑梗死患者血清Pannexin1 和sTREM-1 表达水平及其与病情和预后的相关性研究()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第37卷
期数:
2022年04期
页码:
123-127
栏目:
论著
出版日期:
2022-07-15

文章信息/Info

Title:
Serum Pannexin1 and sTREM-1 Expression Levels in Elderly Patients with Acute Cerebral Infarction and Their Correlation with Disease and Prognosis
文章编号:
1671-7414（2022）04-123-05
作者:
王 淼; 玄洪雷
辽阳市中心医院神经内科，辽宁辽阳 111000
Author(s):
WANG Miao; XUAN Hong-lei
Department of Neurology,the Central Hospital of Liaoyang City, Liaoning Liaoyang 111000，China
关键词:
急性脑梗死; 泛连接蛋白1; 可溶性髓系细胞触发受体1
分类号:
R743.33；R392.11
DOI:
10.3969/j.issn.1671-7414.2022.04.024
文献标志码:
A
摘要:
目的 探讨血清泛连接蛋白1(Pannexin1)、可溶性髓系细胞触发受体1（soluble triggering receptors expressed onmyeloid cells-1，sTREM-1）在老年急性脑梗死（acute cerebral infarction）中的表达及其与病情、预后的关系。方法 选取2019 年6 月～ 2020 年8 月期间在辽阳市中心医院接受治疗的老年急性脑梗死患者126 例作为脑梗死组，根据患者的病情严重程度将其分为轻度组、中度组和重度组，另根据患者的预后情况将其分为预后良好组和预后不良组；另选取同期健康志愿者50 例作为健康对照组。收集所有研究对象的一般资料，采用酶联免疫吸附法检测血清Pannexin1 和sTREM-1 的水平。结果 脑梗死组的血清Pannexin1（4.52±2.36mg/ml ）和sTREM1 水平（25.89±7.62pg/ml）高于健康对照组(2.61±0.98mg/ml，11.52±5.38pg/ml)，差异均有统计学意义（t=5.529，12.175，均P =0.000）。重度组、中度组和轻度组的血清Pannexin1（5.68±2.48mg/ml， 4.62±2.15mg/ml，3.28±1.26mg/ml）和sTREM-1 水平（31.00±8.82pg/ml ，26.02±8.01pg/ml，20.87±6.58pg/ml ）逐渐降低，差异有统计学意义（F=12.956，15.275，均P =0.000）。预后不良组的血清Pannexin1（6.00±2.63mg/ml）和sTREM-1 水平（33.73±9.42pg/ml）高于预后良好组（3.78±1.31mg/ml，21.97±7.15pg/ml），差异有统计学意义（t=6.338 ，7.805，均P =0.000）。血清Pannexin1 和sTREM-1 预测急性脑梗死患者预后不良的ROC 曲线下面积分别为0.767 和0.811。二者联合后敏感度为90.48%，较单一指标的敏感度有了大幅度提高，且约登指数也提高至0.619。结论 血清Pannexin1 和sTREM-1 的表达水平与老年急性脑梗死患者的病情严重程度以及预后密切相关，二者联合对患者的预后有较高的预测价值。
Abstract:
Objective To investigate the expression of serum Pannexin1 and soluble triggering receptors expressed on myeloid cells-1 (sTREM-1) in elderly patients with acute cerebral infarction and its relationship with the condition and prognosis. Methods 126 elderly patients with acute cerebral infarction who were treated in the Central Hospital of Liaoyang City from June 2019 to August 2020 were selected as the cerebral infarction group, and the patients were divided into mild group, moderate group and severe group according to the severity of their illness. According to the prognosis of patients, they were divided into good prognosis group and poor prognosis group;In addition, 50 healthy volunteers who had a physical examination in our hospital during the same period were selected as the healthy control group.The general data of all subjects were collected, and the levels of serum Pannexin1 and sTREM-1 were detected by enzyme-linked immunosorbent assay. Results The levels of serum Pannexin1 (4.52±2.36mg/ml ) and sTREM-1 (25.89±7.62pg/ml ) in the cerebral infarction group were higher than those in healthy control group(2.61±0.98mg/ml，11.52±5.38pg/ml ), the differences were statistically significant (t=5.529，12.175, all P=0.000).The levels of serum Pannexin1 (5.68±2.48mg/ml, 4.62±2.15mg/ml, 3.28±1.26mg/ml) and sTREM-1 (31.00±8.82pg/ml, 26.02±8.01pg/ml, 20.87±6.58pg/ml ) in severe group, moderate group and mild group were decreased gradually,the differences were statistically significant (F=12.956, 15.275, all P=0.000).The levels of serum Pannexin1 (6.00±2.63mg/ml) and sTREM-1 (33.73±9.42pg/ml) in the poor prognosis group were higher than those in the good prognosis group（3.78±1.31mg/ml, 21.97±7.15pg/ml）, the differences were statistically significant (t=6.338, 7.805, all P=0.000).The area under the ROC curve of serum Pannexin1 and sTREM-1 predicting poor prognosis of patients with acute cerebral infarction was 0.767 and 0.811, respectively. The sensitivity of the combination of the two was 90.48%, which was significantly higher than the sensitivity of a single indicator, and the Youden index had also increased to 0.619. Conclusion The expression levels of serum Pannexin1 and sTREM-1 were closely related to the severity and prognosis of elderly patients with acute cerebral infarction, and the combination of the two has a high predictive value for the prognosis of patients.

参考文献/References:

[1] BARTHELS D, DAS H. Current advances in ischemic stroke research and therapies[J]. Biochimica et Biophysica Acta-Molecular Basis of Disease, 2020,1866(4): 165260.
[2] WU Simiao, WU Bo, LIU Ming, et al. Stroke in China:advances and challenges in epidemiology, prevention,and management[J]. Lancet Neurology, 2019, 18(4):394-405.
[3] BRAININ M. Stroke epidemiology in China: which are the next steps[J]. The Lancet Neurology, 2019, 18(4):325-326.
[4] FREITAS-ANDRADE M, BECHBERGER J F,MACVICAR B, et al. Pannexin1 knockout and blockade reduces ischemic stroke injury in female, but not in male mice[J]. Oncotarget, 2017, 8(23): 36973-36983.
[5] YEUNG A K, PATIL C S, JACKSON M F. Pannexin-1 in the CNS: emerging concepts in health and disease[J].Journal of Neurochemistry, 2020, 154(5): 468-485.
[6] LEMARI? J, GIBOT S. Soluble triggering receptor expressed on myeloid cells-1: diagnosis or prognosis[J].Critical Care Clinics, 2020, 36(1): 41-54.
[7] 戴道鹏, 沈迎, 张瑞岩, 等. 血清可溶性髓样细胞触发受体-1 水平对冠状动脉粥样斑块进展的预测价值[J]. 国际心血管病杂志, 2016, 43(5): 303-306. DAI Daopeng, SHEN Ying, ZHANG Ruiyan, et al.Value of serum soluble triggering receptor expressed on myeloid cells-1 level for predicting coronary plaque progression [J]. International Journal of Cardiovascular Disease,2016,43(5):303-306.
[8] 中华医学会神经病学分会, 中华医学会神经病学分会脑血管病学组. 中国急性缺血性脑卒中诊治指南2018[J]. 中华神经科杂志, 2018, 51(9): 666-682. Chinese Society of Neurology, Chinese Stroke Society,Chinese Medical Associations. Chinese guidelines for diagnosis and treatment of acute ischemic stroke 2018[J]. Chinese Journal of Neurology,2018,51(9):666-682.
[9] WANG Wenzhi, JIANG Bin, SUN Haixin, et al.Prevalence, incidence, and mortality of stroke in China:results from a nationwide Population-Based survey of 480 687 adults[J]. Circulation, 2017, 135(8): 759-771.
[10] 赵曼, 赵雅, 王禺, 等. 血清HCY, Cys C 和NTproBNP检测在急性脑梗死诊断及预后中的应用研究[J]. 现代检验医学杂志, 2019, 34(3): 70-73. ZHAO Man,ZHAO Ya,WANG Yu,et al. Clinical application of serum HCY,Cys C and NT-proBNP joint detection in diagnosis and prognosis of acute cerebral infarction [J]. Journal of Modern Laboratory Medicine,2019,34(3):70-73.
[11] KAMTCHUM-TATUENE J, JICKLING G C. Blood biomarkers for stroke diagnosis and management[J].Neuromolecular Medicine, 2019, 21(4): 344-368.
[12] SHAN Yisi, NI Yaohui, GAO Zhiwei. Pannexin-1 channel regulates ATP release in epilepsy[J].Neurochemical Research, 2020, 45(5): 965-971.
[13] GOOD M E, EUCKER S A, LI Jun, et al. Endothelial cell Pannexin1 modulates severity of ischemic stroke by regulating cerebral inflammation and myogenic tone[J]. JCI Insight, 2018, 3(6): 96272.
[14] WEI Ruili, BAO Wangxiao, HE Fangping, et al.Pannexin1 channel inhibitor (10panx) protects against transient focal cerebral ischemic injury by inhibiting RIP3 expression and inflammatory response in rats[J].Neuroscience, 2020, 437: 23-33.
[15] 刘小曼, 臧福才, 郎明非, 等．Pannexin-1 在急性缺血性脑卒中中的研究进展[J]. 卒中与神经疾病,2020, 27(4): 542-545. LIU Xiaoman, ZANG Fucai, LANG Mingfei,et al . Research progress of pannex in -1 in acute ischemic stroke[J]. Stroke and Nervous Diseases,2020,27(4):542-545.
[16] 刘美霞, 周龙. 缺血性脑卒中患者血清Pannexin1水平及其临床意义[J]. 中国现代医学杂志, 2020,30(8): 110-113. LIU Meixia, ZHOU Long. Serum Pannexin1 level in patients with ischemic stroke and its clinical significance [J]. China Journal of Modern Medicine,2020,30(8):110-113.
[17] PATOULIAS D, KALOGIROU M S, PATOULIAS I. Triggering receptor expressed on myeloid cells-1(TREM-1) and its soluble in the plasma form(sTREM-1) as a diagnostic biomarker in neonatal sepsis[J]. Folia Medica Cracoviensia, 2018, 58(2): 15-19.
[18] LIANG Yubin, SONG Pingping, ZHU Yunhui, et al.TREM-1-targeting LP17 attenuates cerebral ischemiainduced neuronal injury by inhibiting oxidative stress and pyroptosis[J]. Biochemical and Biophysical Research Communications, 2020, 529(3): 554-561.
[19] XU Pengfei, ZHANG Xiaohan, LIU Qian, et al. Microglial TREM-1 receptor mediates neuroinflammatory injury via interaction with SYK in experimental ischemic stroke[J]. Cell Death & Disease, 2019, 10(8): 555.
[20] GUERRERO W R, GOLUBCZYK D, WALCZAK P. Thrombus imaging in acute stroke[J]. Stroke, 2019,50(8): 1948-1949.
[21] WANG H M, GAO J H, LU J L. Pravastatin improves atherosclerosis in mice with hyperlipidemia by inhibiting TREM-1/DAP12[J]. European Review for Medical and Pharmacological Sciences, 2018, 22(15):4995-5003.
[22] SOLANKI A, BHATT L K, Johnston TP. Evolving targets for the treatment of atherosclerosis[J].Pharmacol Ther,2018 ,187:1-12.

相似文献/References:

[1]何凤娥,刘依霜,段春艳.ACI患者TEG各项参数与PAgT和D-二聚体之间的相关性[J].现代检验医学杂志,2017,32(01):134.[doi:10.3969/j.issn.1671-7414.2017.01.037]
　HE Feng-e,LIU Yi-shuang,DUAN Chun-yan.Correlation between TEG Parameters and PAgT and D-Dimer in Patients with ACIH[J].Journal of Modern Laboratory Medicine,2017,32(04):134.[doi:10.3969/j.issn.1671-7414.2017.01.037]
[2]王 禺,李秀娥,赵 曼,等.急性脑梗死患者的NT-proBNP和Hcy及部分凝血纤溶指标变化的临床意义[J].现代检验医学杂志,2017,32(03):89.[doi:10.3969/j.issn.1671-7414.2017.03.024]
　WANG Yu,LI Xiu-e,ZHAO Man,et al.Clinical Significance of Changes in NT-proBNP，Hcy and Partial Coagulation-Fibrinolytic Indexes with Acute Cerebral Infarction[J].Journal of Modern Laboratory Medicine,2017,32(04):89.[doi:10.3969/j.issn.1671-7414.2017.03.024]
[3]刘学芳,霍 豆,赵 茜,等.急性脑梗死患者血清VEGF,SAA和Lp-PLA2联合检测的临床研究[J].现代检验医学杂志,2018,33(04):76.[doi:10.3969/j.issn.1671-7414.2018.04.020]
　LIU Xue-fang,HUO-Dou,ZHAO-Qian,et al.Diagnostic Value of Combined Detection of VEGF,SAA and Lp-PLA2 for Acute Cerebral Infarction[J].Journal of Modern Laboratory Medicine,2018,33(04):76.[doi:10.3969/j.issn.1671-7414.2018.04.020]
[4]张同庆,杨文东.急性脑梗死并发幽门螺旋杆菌感染者血脂水平及氧化应激反应的观察[J].现代检验医学杂志,2018,33(02):66.[doi:10.3969/j.issn.1671-7414.2018.02.001]
　ZHANG Tong-qing,YANG Wen-dong.Observation of Serum Lipid Level and Oxidative Stress Response in Patients with Acute Cerebral Infarction Complicated with Helicobacter Pylori Infection[J].Journal of Modern Laboratory Medicine,2018,33(04):66.[doi:10.3969/j.issn.1671-7414.2018.02.001]
[5]刘 莉,张 军.急性脑梗死患者血清hs-CRP,TNF-α和TIMP-1水平检测在疾病预后评估中的应用价值[J].现代检验医学杂志,2018,33(05):87.[doi:10.3969/j.issn.1671-7414.2018.05.023]
　LIU Li,ZHANG Jun.Value of Serum hs-CRP,TNF-α and TIMP-1 Levels in Prognostic Evaluation of Patients with Acute Cerebral Infarction[J].Journal of Modern Laboratory Medicine,2018,33(04):87.[doi:10.3969/j.issn.1671-7414.2018.05.023]
[6]陈 霏,王 碧.血清PDGF 及SDF-1 水平与急性脑梗死患者脑侧支循环建立的相关性研究[J].现代检验医学杂志,2020,35(01):82.[doi:10.3969/j.issn.1671-7414.2020.01.022]
　CHEN Fei,WANG Bi.Study on the Correlation between Levels of MMP-9 and SDF-1 in Serumand the Establishment of Cerebral Collateral Circulation inPatients with Acute Cerebral Infarction[J].Journal of Modern Laboratory Medicine,2020,35(04):82.[doi:10.3969/j.issn.1671-7414.2020.01.022]
[7]杨 华a,李致文b,曹明善a,等.急性脑梗死患者血清miR-130a 水平表达与溶栓后再出血转化风险的预测模型及验证[J].现代检验医学杂志,2022,37(06):28.[doi:10.3969/j.issn.1671-7414.2022.06.006]
　YANG Huaa,LI Zhi-wenb,CAO Ming-shana,et al.Prediction Model and Validation of Serum miR-130a Expression in Patients with Acute Cerebral Infarction and the Risk of Hemorrhagic Transformation after Thrombolysis[J].Journal of Modern Laboratory Medicine,2022,37(04):28.[doi:10.3969/j.issn.1671-7414.2022.06.006]
[8]聂晓慧,薛 茜,寇文辉,等.急性脑梗死患者外周血LncRNA-NEAT1，miR-93-5p 表达水平及临床意义[J].现代检验医学杂志,2022,37(06):94.[doi:10.3969/j.issn.1671-7414.2022.06.017]
　NIE Xiao-hui,XUE Qian,KOU Wen-hui,et al.Expression Level and Clinical Significance of Peripheral Blood LncRNANEAT1 and miR-93-5p in Patients with Acute Cerebral Infarction[J].Journal of Modern Laboratory Medicine,2022,37(04):94.[doi:10.3969/j.issn.1671-7414.2022.06.017]
[9]张 磊,赵腾跃,李 彬.急性脑梗死患者血清Beclin-1，3-MST，TSP-1 表达水平及与病情和预后的相关性研究[J].现代检验医学杂志,2022,37(06):124.[doi:10.3969/j.issn.1671-7414.2022.06.023]
　ZHANG Lei,ZHAO Teng-yue,LI Bin.Serum Beclin-1, 3-MST and TSP-1 Expression Levels in Patients with Acute Cerebral Infarction and Their Correlation with Disease Condition and Prognosis[J].Journal of Modern Laboratory Medicine,2022,37(04):124.[doi:10.3969/j.issn.1671-7414.2022.06.023]
[10]牛立远a,刘会雪a,史立彬b.急性脑梗死患者血清CCR5 和miR-211-5p 表达水平及其临床意义[J].现代检验医学杂志,2023,38(01):128.[doi:10.3969/j.issn.1671-7414.2023.01.024]
　NIU Li-yuana,LIU Hui-xuea,SHI Li-binb.Expression Levels and Their Clinical Significance of Serum CCR5 and miR-211-5p in Patients with Acute Cerebral Infarction[J].Journal of Modern Laboratory Medicine,2023,38(04):128.[doi:10.3969/j.issn.1671-7414.2023.01.024]

备注/Memo

备注/Memo:
作者简介：王淼（1981-），女，硕士研究生，副主任医师，研究方向：神经免疫、脑血管病方向，E-mail：wangmiao856723@163.com。
更新日期/Last Update: 1900-01-01