[1]杨春光a,刘海红a,王凤东a,等.特发性肺动脉高压患儿血清miR-182 和ICAM-1 表达水平及其与预后相关性分析[J].现代检验医学杂志,2022,37(05):23-27+43.[doi:10.3969/j.issn.1671-7414.2022.05.006]
　YANG Chun-guanga,LIU Hai-honga,WANG Feng-donga,et al.Analysis of Serum miR-182 and ICAM-1 Expression Levels in Children with Idiopathic Pulmonary Hypertension and Their Correlation with Prognosis[J].Journal of Modern Laboratory Medicine,2022,37(05):23-27+43.[doi:10.3969/j.issn.1671-7414.2022.05.006]
点击复制

特发性肺动脉高压患儿血清miR-182 和ICAM-1 表达水平及其与预后相关性分析()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第37卷
期数:
2022年05期
页码:
23-27+43
栏目:
论著
出版日期:
2022-09-15

文章信息/Info

Title:
Analysis of Serum miR-182 and ICAM-1 Expression Levels in Children with Idiopathic Pulmonary Hypertension and Their Correlation with Prognosis
文章编号:
1671-7414（2022）05-023-06
作者:
杨春光a; 刘海红a; 王凤东a; 王晓静a; 张学郁b; 张海波c
承德市妇幼保健院 a. 新生儿科；b. 检验科；c. 儿科，河北承德067020
Author(s):
YANG Chun-guanga;  LIU Hai-honga;  WANG Feng-donga;  WANG Xiao-jinga;  ZHANG Xue-yub;  ZHANG Hai-boc
a. Department of Neonatology; b.Department of Clinical Laboratory; c.Department of Pediatrics, Chengde Maternal and Child Health Hospital, Hebei Chengde 067020, China
关键词:
特发性肺动脉高压; 微小核糖核酸-182; 细胞间黏附分子-1
分类号:
R725.4；R392.11
DOI:
10.3969/j.issn.1671-7414.2022.05.006
文献标志码:
A
摘要:
目的　探讨血清微小核糖核酸（micro RNA，miR）-182、细胞间黏附分子（intercellular adhesion molecule-1，ICAM）-1 在特发性肺动脉高压（idiopathic pulmonary arterial hypertension，IPAH）患儿中的表达及其预后预测价值。方法　选取2016 年6 月～ 2020 年12 月就诊于承德市妇幼保健院肺动脉高压中心的76 例IPAH 患儿为研究对象（观察组），另选取同期在医院进行体检的健康儿童30 例作为对照组，比较二组血清miR-182，ICAM-1 水平。收集IPAH 患儿常规实验室检查、心脏超声检查及心导管检查等资料，并进行随访，随访的终点事件定义为死亡或行肺移植治疗，根据患儿预后情况将其分为存活组和死亡组。采用Logistic 回归分析筛选影响IPAH 患儿预后的危险因素，绘制受试者工作特征（receiver operating characteristic，ROC）曲线评价血清miR-182，ICAM-1 单独及联合检测对IPAH 患儿预后的预测价值。结果　观察组血清miR-182（1.91±0.20），ICAM-1（305.67±32.46 ng/ml）水平均高于对照组（0.15±0.02，203.22±21.51 ng/ml），差异具有统计学意义（t=-73.624，-33.698，均P ＜ 0.05）。72 例患儿获得随访，其中死亡21 例（29.17%），存活51 例（70.83%）。死亡组NYHA 心功能分级（III ～ IV 级）占比、脑钠肽（brainnatriuretic peptide，BNP）、总胆红素（total bilirubin，TB）、红细胞分布宽度（red cell volume distribution width，RDW）、肺动脉收缩压（systolic pulmonary artery pressure，sPAP）、全肺阻力（total pulmonary resistance，TPR）、miR-182 和ICAM-1 水平均高于存活组，差异具有统计学意义（χ2=4.138，t’=-75.584，t=7.231，6.197，2.464，3.930，t’=6.782，3.328，均P ＜ 0.05）。NYHA 心功能分级（高级）（OR=1.681，95%CI=1.370 ～ 5.128），RDW（OR=2.112，95%CI=1.212 ～ 5.135），sPAP（OR=2.597，95%CI=1.052 ～ 6.1425），miR-182（OR=3.587，95%CI=1.249 ～ 6.125）和ICAM-1（OR=3.634，95%CI=1.135 ～ 5.153）是影响IPAH 患儿预后的危险因素（均P ＜ 0.05）。血清miR-182 和ICAM-1 预测IPAH 患儿预后的曲线下面积（AUC）分别为0.761，0.770，联合预测的AUC 为0.883，优于各指标单独预测（Z= -3.236，-3.211，均P ＜ 0.05）。结论　血清miR-182 和ICAM-1 在IPAH 患儿中呈现异常高表达，二者与患儿预后存在一定关系，在一定程度上可作为有效预测指标。
Abstract:
Objective　To investigate the expression of serum micro RNA（miR）-182 and intercellular adhesion molecule-1 (ICAM)-1 in children with idiopathic pulmonary arterial hypertension (IPAH) and their the prognostic value of ICAM-1 in children with idiopathic pulmonary arterial hypertension (IPAH). Methods　76 children with IPAH who attended the Pulmonary Hypertension Center of Chengde Maternal and Child Health Hospital from June 2016 to November 2020 were selected for the study (observation group), and another 30 healthy children who underwent physical examination at the hospital during the same period were selected as the control group to compare the serum miR-182 and ICAM-1 levels in the two groups. Routine laboratory tests, cardiac ultrasonography and cardiac catheterization were collected from children with IPAH and followed up with an endpoint event defined as death or undergoing lung transplantation, and the children were divided into survival and death groups according to their prognosis. Logistic regression analysis was used to screen the risk factors affecting the prognosis of children with IPAH, and receiver operating characteristic(ROC）curves were drawn to evaluate the individual and combined predictive value of serum miR-182 and ICAM-1 on the prognosis of children with IPAH. Results　The levels of serum miR-182 (1.91±0.20) and ICAM-1 levels (305.67±2.46 ng/ml) were higher in the observation group than in the control group (0.15±0.02, 203.22±21.51 ng/ml), and the differences were statistically significant (t=-73.624，-33.698, all P<0.05). 72 children were followed up, of which 21 (29.17%) died and 51 (70.83%) survived. The percentage of NYHA cardiac function class (III ～ IV) in the death group, brain natriuretic peptide (BNP), total bilirubin (TB), red blood cell distribution width (RDW), systolic pulmonary artery pressure (sPAP), total pulmonary resistance (TPR), miR-182 and ICAM-1 levels were higher than those in the survivor group, all with statistically significant differences ( χ2=4.138，t’=-75.584, t=7.231, 6.197, 2.464, 3.930, t’=6.783， 3.328, all P<0.05). NYHA cardiac function class (advanced) （OR=1.681，95%CI=1.370 ～ 5.128），RDW（OR=2.112， 95%CI=1.212 ～ 5.135），sPAP（OR=2.597，95%CI=1.052 ～ 6.1425），miR-182（OR=3.587，95%CI=1.249 ～ 6.125） and ICAM-1（OR=3.634，95%CI=1.135 ～ 5.153） were risk factors affecting the prognosis of children with IPAH (all P<0.05). The area under the curve (AUC) of serum miR-182, ICAM-1 to predict the prognosis of children with IPAH was 0.761 and 0.770, respectively, and the combined AUC was 0.883, which was better than each index alone (Z= -3.236，-3.211, all P<0.05). Conclusion　Serum miR-182 and ICAM-1 show abnormally high expression in children with IPAH, both of which would be related to the prognosis of the children and could be used as effective predictors to some extent.

参考文献/References:

[1] HOEPER M M, PAUSCH C, GR?NIG E, et al. Idiopathic pulmonary arterial hypertension phenotypes determined by cluster analysis from the COMPERA registry[J]. The Journal of Heart and Lung Transplantation, 2020, 39(12): 1435-1444.
[2] OGAWA A, SATOH T, TAMURA Y, et al. Survival of Japanese patients with idiopathic/heritable pulmonary arterial hypertension[J]. The American Journal of Cardiology, 2017, 119(9): 1479-1484.
[3] 刘倩, 张陈, 李强强, 等. 特发性肺动脉高压患儿预后及其危险因素分析[J]. 中华儿科杂志, 2018,56(1):23-28.
LIU Qian, ZHANG Chen, LI Qiangqiang, et al.Analysis of prognosis and associated risk factors in pediatric idiopathic pulmonary arterial hypertension [J].Chinese Journal of Pediatrics, 2018, 56(1):23-28.
[4] BERTELOOT L, PROISY M, JAIS J P, et al. Idiopathic,heritable and veno-occlusive pulmonary arterial hypertension in childhood: computed tomography angiography features in the initial assessment of the disease[J]. Pediatric Radiology, 2019, 49(5): 575-585.
[5] KAM C W, RUIZ F E. Opportunities and challenges of pharmacotherapy for pulmonary arterial hypertension in children[J]. Pediatr Pulmonol, 2021 , 56(3):593-613.
[6] CHANG Hao, LIU Yuehong, WANG Lili, et al.MiR-182 promotes cell proliferation by suppressing FBXW7 and FBXW11 in non-small cell lung cancer[J].American Journal of Translational Research, 2018,10(4): 1131-1142.
[7] ZHAO Nan, LU Chunyuan, LIU Liang, et al.Intercellular adhesion molecule-1 and S-100β in sevoflurane combined with epidural anesthesia for radical resection of lung cancer[J]. Oncology Letters,2020, 19(2): 1544-1550.
[8] WU Huiliang, ZHANG Jing. MiR-126 in peripheral blood mononuclear cells negatively correlates with risk and severity and is associated with inflammatory cytokines as well as intercellular adhesion molecule-1 in patients with coronary artery disease[J]. Cardiology,2018, 139(2): 110-118.
[9] GALI? N, HUMBERT M, VACHIERY J L, et al. 2015 ESC/ERS guidelines for the diagnosis and treatment of pulmonary hypertension:the joint task force for the diagnosis and treatment of pulmonary hypertension of the European Society of Cardiology(ESC)and the European Respiratory Society(ERS):endorsed by:Association for European Paediatric and Congenital Cardiology(AEPC), International Society for Heart and Lung Transplantation(ISHLT)[J]. European Heart Journal, 2016, 37(1): 67-119.
[10] ABMAN S H, HANSMANN G, ARCHER S L, et al.Pediatric pulmonary hypertension: Guidelines from the American Heart Association and American Thoracic Society[J]. Circulation, 2015, 132(21): 2037-2099.
[11] WANG Xiaojian, LIAN Tianyu, JIANG Xin, et al.Germline BMP9 mutation causes idiopathic pulmonary arterial hypertension[J]. The European Respiratory Journal, 2019, 53(3): 1801609.
[12] 朱珊英, 朱国斌. 肺动脉高压发病机制新进展[J]. 心血管病学进展, 2020, 41(3):292-295.
ZHU Shanying, ZHU Guobin. Pathogenesis of pulmonary hypertension[J]. Advances in Cardiovascular Diseases, 2020, 41(3):292-295.
[13] HU Yijie, CHI L, KUEBLER W M, et al. Perivascular inflammation in pulmonary arterial hypertension[J].Cells (Basel, Switzerland), 2020, 9(11): 2338.
[14] ZHANG Xingzeng, MA Genshun, LIU Jianchao, et al.MicroRNA-182 promotes proliferation and metastasis by targeting FOXF2 in triple-negative breast cancer[J].Oncology Letters, 2017, 14(4): 4805-4811.
[15] JIA Li, LUO Shihua, REN Xiang, et al. MiR-182 and miR-135b mediate the tumorigenesis and invasiveness of colorectal cancer cells via targeting ST6GALNAC2 and PI3K/AKT pathway[J]. Digestive Diseases and Sciences, 2017, 62(12): 3447-3459.
[16] HE Zongze, YOU Chao, ZHAO Dongdong. Long noncoding RNA UCA1/miR-182/PFKFB2 axis modulates glioblastoma-associated stromal cells-mediated glycolysis and invasion of glioma cells[J]. Biochemical and Biophysical Research Communications, 2018,500(3): 569-576.
[17] 郭洁, 池水侠.急性缺血性脑卒中患者血清miRNA-181c-5p,miRNA-340-5p 和miRNA146a 水平表达检测的诊断价值研究[J]. 现代检验医学杂志, 2021, 36(5): 43-45, 89.
GUO Jie, CHI Shuixia. Diagnostic Value of miRNA-181c-5p, miRNA-340-5p and miRNA146a levels in patients with acute ischemic stroke [J]. Journal of Modern Laboratory Medicine,2021,36(5):43-45,89.
[18] 姚少侠, 罗文强. 心房颤动患者血浆miRNA-1 和miRNA-155 检测在复律后复发预测的应用研究[J].现代检验医学杂志, 2018, 33(3):95-98, 102.
YAO Shaoxia, LUO Wenqiang. Application of plasma miRNA-1 and miRNA-155 in predicting relapse after cardioversion for atrial fibrillation [J]. Journal of Modern Laboratory Medicine,2018,33(3):95-98,102.
[19] BABICHEVA A, AYON R J, ZHAO Tengteng, et al.MicroRNA-mediated downregulation of K+ channels in pulmonary arterial hypertension[J]. American Journal of Physiology-Lung Cellular and Molecular Physiology,2020, 318(1): L10-L26.
[20] 郭天聪, 宁欣, 倪楠, 等.miR-182 在肺动脉高压患者中的表达分析及对HPVSMC 细胞增殖的影响[J].重庆医学, 2019, 48(2):229-232.
GUO Tiancong, NING Xin, NI Nan, et al. Expression of miR-182 in patients with pulmonary hypertension and its effects on cell proliferation of HPVSMC [J].Chongqing Medicine, 2019, 48(2):229-232.
[21] HUERTAS A, TU L, HUMBERT M, et al. Chronic inflammation within the vascular wall in pulmonary arterial hypertension: more than a Spectator[J].Cardiovascular Research, 2020, 116(5): 885-893.
[22] THAKKAR V, PATTERSON K A, STEVENS W, et al.Increased serum levels of adhesion molecules ICAM-1 and VCAM-1 in systemic sclerosis are not specific for pulmonary manifestations[J]. Clinical Rheumatology,2018, 37(6): 1563-1571.
[23] 钟士杰, 邓堂, 林开文, 等. 车前草提取物对缺氧性肺动脉高压SD 大鼠模型肺动脉压力、肺功能及炎症因子的影响[J]. 现代生物医学进展, 2021, 21 (11):2035-2039.
ZHONG Shijie, DENG Tang, LIN Kaiwen, et al.Plantain extract on SD rat model of hypoxic pulmonary hypertension pulmonary artery pressure, lung function and the influence of inflammatory factors [J]. Progress in Modern Biomedicine, 2021, 21(11):2035-2039.
[24] 朱燕, 张陈, 张丹, 等. 儿童特发性肺动脉高压预后相关因素的分析[J].心肺血管病杂志, 2015, 34(6):456-460, 464.
ZHU Yan, ZHANG Chen, ZHANG Dan, et al.Predictors of survival in pediatric patients with idiopathic pulmonary hypertension [J]. Journal of Cardiovascular and Pulmonary Diseases, 2015, 34(6):456-460, 464.

备注/Memo

备注/Memo:
基金项目：河北省医学科学研究课题项目（20200184）。
作者简介：杨春光（1982-），女，本科，主治医师，研究方向：新生儿疾病，E-mail：haytes3@21cn.com。
更新日期/Last Update: 2022-09-15