[1]邝 辉,尚 娟,努尔比叶,等.新疆维吾尔族静脉血栓患者Th17/Treg 细胞及相关细胞因子和细胞黏附分子整合素β 表达水平及临床意义[J].现代检验医学杂志,2022,37(05):28-32+75.[doi:10.3969/j.issn.1671-7414.2022.05.007]
　KUANG Hui,SHANG Juan,NUER·Biye,et al.Expression Levels and Clinical Significance of Th17/Treg Cells, Related Cytokines and Cell Adhesion Molecule Integrin β in Uygur Patients with Venous Thrombosis in Xinjiang[J].Journal of Modern Laboratory Medicine,2022,37(05):28-32+75.[doi:10.3969/j.issn.1671-7414.2022.05.007]
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新疆维吾尔族静脉血栓患者Th17/Treg 细胞及相关细胞因子和细胞黏附分子整合素β 表达水平及临床意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第37卷
期数:
2022年05期
页码:
28-32+75
栏目:
论著
出版日期:
2022-09-15

文章信息/Info

Title:
Expression Levels and Clinical Significance of Th17/Treg Cells, Related Cytokines and Cell Adhesion Molecule Integrin β in Uygur Patients with Venous Thrombosis in Xinjiang
文章编号:
1671-7414（2022）05-028-06
作者:
邝 辉1;  尚 娟1;  努尔比叶1;  郑伟萍2;  杨 凡2
1. 喀什地区第二人民医院检验科，新疆喀什 844000；2. 上海市同济医院检验科，上海 200065
Author(s):
KUANG Hui1;  SHANG Juan1;  NUER·Biye1;  ZHENG Wei-ping2;  YANG Fan2
1.Department of Clinical Laboratory, the Second People’s Hospital of Kashi，Xinjiang Kashi 844000，China； 2. Department of Clinical Laboratory, Shanghai Tongji Hospital，Shanghai 200065，China
关键词:
静脉血栓栓塞症; 辅助性T 细胞17/ 调节性T 细胞; 细胞因子; 整合素
分类号:
R543.6；R392.11
DOI:
10.3969/j.issn.1671-7414.2022.05.007
文献标志码:
A
摘要:
目的　探讨辅助性T 细胞 17（T helper 17 cells，Th17）/ 调节性T 细胞（regulatory T cells，Treg）及相关细胞因子和细胞黏附分子整合素β 在新疆维吾尔族静脉血栓患者中的表达及其对静脉血栓栓塞症（venous thromboembolism，VTE）的临床诊断价值分析。方法　回顾性选择2017 年1 月～ 2019 年6 月喀什地区第二人民医院确诊的新疆维吾尔族VTE 患者63 例作为研究对象；同时以70 例健康体检人群作为对照。观察Th17/Treg 细胞及其相关细胞因子IL-2，IL-6，IL-10，IL-17，TNF-α 以及细胞黏附分子整合素β 的表达情况；分析Th17/Treg 细胞及细胞黏附分子整合素β 对VTE 的临床预测价值。结果　VTE 组患者Th17（32.65%±11.56%）和Treg 细胞（12.16%±5.84%）及相关细胞因子IL-2（7.58±3.61pg/ml），IL-6（10.64±5.12 pg/ml），IL-10（8.15±3.87 pg/ml），IL-17（9.37±4.36 pg/ml），TNF-α（10.95±5.83 pg/ml） 表达及Th17/Treg 比例（2.68±1.23） 均较对照组（20.98%±10.14%，9.85%±4.27%，6.45±2.34pg/ml，5.32±2.48pg/ml，6.49±2.85pg/ml，3.15±1.84pg/ml，8.12±3.98pg/ml，2.02±0.95） 明显升高， 差异均有统计学意义（t=2.163 ～ 10.909，均P<0.05）。VTE 组患者细胞黏附分子整合素β1（16.59%±8.42%），β2（96.58%±28.64%），β3（15.75%±5.26%）均较对照组（10.53 %±4.16%， 85.75 %±19.83%，8.45 %±2.01%）明显升高，差异均有统计学意义（t=2.556 ～ 10.774，均P<0.05）。ROC 曲线显示，Th17 和Treg 细胞及Th17/Treg 比值诊断VTE 的AUC 值分别为0.826，0.809 和0.745，当最佳截断值分别取27.45%，11.26% 和2.51 时，诊断灵敏度分别为76.19%，79.36% 和69.84%，特异度分别为87.30%，82.54% 和80.95%。整合素β1，β2 和β3 诊断VTE 的AUC值分别为0.723，0.706 和0.698，当最佳截断值分别取13.97%，93.75% 和12.88% 时，诊断灵敏度分别为73.02%，77.78% 和71.43%，特异度分别为68.25%，63.49% 和66.67%。结论　维吾尔族静脉血栓患者存在Th17/Treg 平衡失调及相关细胞因子和细胞黏附分子整合素β 表达增高；Th17/Treg 细胞和细胞黏附分子整合素β 对VTE 具有较高的诊断价值，可将其作为VTE 的辅助诊断指标。
Abstract:
Objective　To investigate the expression of T helper 17 cells/regulatory T cells（Th17/Treg）, related cytokines and cell adhesion molecule integrin β in Xinjiang Uygur patients with venous thrombosis and its clinical diagnostic value for VTE. Methods　63 cases Uygur VTE patients diagnosed in the Second People’s Hospital of Kashi from January 2017 to June 2019 were retrospectively selected as the study subjects. At the same time, 70 healthy people were taken as control. The expression of Th17/Treg cells and their related cytokines IL-2, IL-6, IL-10, IL-17, TNF-α and cell adhesion molecule integrin β were observed.The clinical predictive value of Th17/Treg cells and cell adhesion molecule integrin β on VTE was analyzed. Results　 The expression of Th17（32.65%±11.56%） and Treg （12.16%±5.84%）cells and related cytokines IL-2（7.58±3.61pg/ml），IL-6（10.64±5.12 pg/ml），IL-10（8.15±3.87 pg/ml），IL-17（9.37±4.36 pg/ml），TNF-α （10.95±5.83 pg/ml）and Th17/Treg ratio （2.68±1.23）in VTE group were significantly higher than those in control group （20.98%±10.14%，9.85%±4.27%，6.45±2.34pg/ml，5.32±2.48pg/ml，6.49±2.85pg/ml，3.15±1.84pg/ml，8.12±3.98pg/ ml，2.02±0.95），the differences were statistically significant （t= 2.163 ～ 10.909, all P<0.05）. Cell adhesion molecules integrin β1（16.59%±8.42%）, β2（96.58%±28.64%）, β3（15.75%±5.26%）were significantly increased in VTE group compared with control group（10.53 %±4.16%，85.75 %±19.83%，8.45 %±2.01%），the differences were statistically significant（t=2.556 ～ 10.774，all P<0.05）. ROC curve showed that AUC values of Th17 and Treg cells and Th17/Treg ratio for diagnosing VTE were 0.826, 0.809 and 0.745, respectively. When the optimal truncation values were 27.45%, 11.26% and 2.51, the diagnostic sensitivities were 76.19%, 79.36% and 69.84%, respectively. The specificity was 87.30%, 82.54% and 80.95%, respectively, and the AUC values of integrin β1, β2 and β3 were 0.723, 0.706 and 0.698, respectively. When the optimal truncation values were 13.97%, 93.75% and 12.88%, the diagnostic sensitivities were 73.02%, 77.78% and 71.43%, respectively, and the specificity were 68.25%, 63.49% and 66.67%, respectively. Conclusion　Th17/Treg imbalance and increased expression of related cytokines and cell adhesion molecule integrin β were found in Uygur patients with venous thrombosis.Th17/Treg cells and the cell adhesion molecule integrin β have high diagnostic value for VTE, which can be used as an auxiliary diagnostic index of VTE.

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备注/Memo

备注/Memo:
基金项目：新疆维吾尔自治区自然基金资助项目（2016D01C089）。
作者简介：邝辉（1968-），女，本科，主任检验技师，研究方向：临床免疫学和血液学。
通讯作者： 杨凡（1972-），男，医学博士，副主任技师，研究方向：心血管疾病和肿瘤的免疫学研究及检测，E-mail：ojr6157ws@163.com。
更新日期/Last Update: 2022-09-15