[1]李慧敏,杨 琳.稽留流产绒毛组织中APPL1，Nrf2 和HO-1 的水平表达与氧化应激的相关性研究[J].现代检验医学杂志,2022,37(05):33-38.[doi:10.3969/j.issn.1671-7414.2022.05.008]
　LI Hui-min,YANG Lin.Study on the Correlation between APPL1, Nrf2 and HO-1 Expression and Oxidative Stress in Villous Tissue of Aborted Abortion[J].Journal of Modern Laboratory Medicine,2022,37(05):33-38.[doi:10.3969/j.issn.1671-7414.2022.05.008]
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稽留流产绒毛组织中APPL1，Nrf2 和HO-1 的水平表达与氧化应激的相关性研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第37卷
期数:
2022年05期
页码:
33-38
栏目:
论著
出版日期:
2022-09-15

文章信息/Info

Title:
Study on the Correlation between APPL1, Nrf2 and HO-1 Expression and Oxidative Stress in Villous Tissue of Aborted Abortion
文章编号:
1671-7414（2022）05-033-06
作者:
李慧敏; 杨 琳
陕西省核工业二一五医院产科，陕西咸阳 712000
Author(s):
LI Hui-min;  YANG Lin
Department of Obstetrics, Shaanxi Provincial Nuclear Industry 215 Hospital, Shaanxi Xianyang 712000, China
关键词:
稽留流产; 蛋白亮氨酸拉链基序1; 核因子E2 相关因子; 血红素加氧酶1; 绒毛组织; 氧化应激
分类号:
R714.21；R392.11
DOI:
10.3969/j.issn.1671-7414.2022.05.008
文献标志码:
A
摘要:
目的　探讨稽留流产绒毛组织中蛋白亮氨酸拉链基序1（leucine zipper motif 1, APPL1）、核因子E2 相关因子2（Nuclear factor-erythroid 2-related factor 2，Nrf2）和血红素加氧酶1（haem oxygenase 1，HO-1）的水平表达与氧化应激的相关性。方法　取46 例稽留流产并行人流术治疗者为实验组，取50 例同期正常早孕并行人工流产术者为对照组，收集两组绒毛组织标本，用放射免疫沉淀法裂解缓冲液(radio immunopreci pitation assay lysis buffer, RIPA) 提取绒毛组织蛋白。用免疫组织化学法检测绒毛组织中APPL1，Nrf2 和HO-1 蛋白表达；用Western blot 检测绒毛组织中APPL1，Nrf2 和HO-1 蛋白水平；酶联免疫吸附法（enzyme linked immunosorbent assay，ELISA）测定绒毛组织中氧化应激指标[ 超氧化物歧化酶（superoxide dismutase，SOD）、活性氧（reactive oxygen species，ROS）及丙二醛（malondialdehyde，MDA）] 水平。Pearson 检验分析实验组绒毛组织中APPL1，Nrf2 和HO-1 蛋白水平与氧化应激指标水平的相关性。结果　实验组绒毛组织中APPL1，Nrf2 和HO-1 表达阳性率分别为21.7%，32.6% 和26.1%，明显低于对照组的56.0%，64.0%，58.0%，差异均有统计学意义（χ2=9.442 ～ 11.759，均P＜ 0.05）；APPL1（3.2±0.9），Nrf2（3.7±0.7）和HO-1（3.5±0.8）表达免疫组化评分亦明显低于对照组（4.6±1.4，5.5±1.7，5.1±1.3），差异有统计学意义（t=5.772 ～ 7.187，均P ＜ 0.05）。实验组绒毛组织中APPL1（1.3±0.1），Nrf2（0.7±0.1）和HO-1（1.1±0.1）蛋白表达水平明显低于对照组（1.6±0.2，1.1±0.0.1，1.4±0.1），差异有统计学意义（t=9.171 ～ 19.579，均P ＜ 0.05）。实验组绒毛组织中ROS（8.4±1.5U/ml），MDA（11.7±2.3nmol/ml） 水平明显高于对照组（3.6±0.5U/ml，5.6±0.8nmol/ml），SOD（23.9±6.8U/ml）水平明显低于对照组（28.1±6.6U/ml），差异均有统计学意义（t=3.070 ～ 21.381，均P ＜ 0.05）。实验组绒毛组织中APPL1，Nrf2 和HO-1 蛋白表达与ROS 水平呈负相关（r=-0.628，-0.510，-0.575，均P ＜ 0.05）；与MDA 水平呈负相关（r=-0.541，-0.489，-0.556，均P ＜ 0.05）；与SOD 水平呈正相关（r=0.359，0.423，0.408，均P ＜ 0.05）。结论　APPL1，Nrf2 和HO-1 在稽留流产绒毛组织中呈低表达，且与氧化应激指标ROS，MDA和SOD水平具有显著相关性，其三者异常表达可能通过介导氧化应激失衡进而诱导了稽留流产的发生。
Abstract:
Objective　To investigate the correlation between the levels of leucine zipper motifs 1 (APPL1), nuclear factorerythroid 2-related factor 2（Nrf2）, heme oxygenase 1 (HO-1) and oxidative stress in villi of aborted abortion. Methods　46 cases of delayed abortion and induced abortion were selected as the experimental group, and 50 cases of normal early pregnancy and induced abortion were selected as the control group. Villus tissue samples of the two groups were collected, and villus tissue protein was extracted by RIPA protein lysis method. The expressions of APPL1, Nrf2 and HO-1 proteins in villus were detected by immunohistochemistry. The protein levels of APPL1, Nrf2 and HO-1 in villus were detected by Western blot. The levels of oxidative stress indexes [superoxide dismutase (SOD), reactive oxygen species (ROS) and malondialdehyde (MDA)] in villus were determined by enzyme-linked immunosorbent assay (ELISA).Pearson correlation test was used to analyze the correlation between APPL1 , Nrf2 and HO-1 proteins expression and oxidative stress indexes in villous tissue of the study group. Results　 The positive rates of APPL1, Nrf2 and HO-1 in villi of experimental group were 21.7%, 32.6% and 26.1%, respectively, which were significantly lower than 56.0%, 64.0% and 58.0% in control group (χ2=9.448 ～ 11.759, all P ＜ 0.05). The expression of APPL1(3.2±0.9), Nrf2 (3.7±0.7) and HO-1 (3.5±0.8) were also significantly lower than the control group（4.6±1.4, 5.5±1.7, 5.1±1.3）, the differences were statistically significant (t=5.772～7.187, all P＜0.05). The expression levels of APPL1 (1.3±0.1), Nrf2 (0.7±0.1) and HO-1 (1.1±0.1 ) in the villus tissues of the experimental group were significantly lower than those of the control group（1.6±0.2，1.1±0.0.1，1.4±0.1）, the differences were statistically significant (t=9.171 ～ 19.579, all P ＜ 0.05). The ROS (8.4±1.5U/ml) and MDA (11.7±2.3nmol/ml) levels in villous tissue of experimental group were significantly higher than those in control group（3.6±0.5U/ml，5.6±0.8nmol/ml）, while SOD (23.9±6.8U/ml) levels were significantly lower than those in control group(28.1±6.6U/ml), the differences were statistically significant (t=3.070 ～ 21.381, all P ＜ 0.05). In the experimental group, the expressions of APPL1, Nrf2 and HO-1 were negatively correlated with ROS levels (r=-0.628, -0.510, -0.575, all P ＜ 0.05). There was negative correlation with MDA level (r=-0.541, -0.489, -0.556, all P ＜ 0.05).And SOD level were positively correlated (r=0.359, 0.423, 0.408, all P ＜ 0.05). Conclusion　APPL1, Nrf2 and HO-1 showed low expression in the villous tissue of delayed abortion, and were significantly correlated with the levels of oxidative stress indexes ROS, MDA and SOD. The abnormal expression of APPL1, Nrf2 and HO-1 may induce the occurrence of delayed abortion by mediating oxidative stress imbalance.

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备注/Memo

备注/Memo:
基金项目：陕西省重点研发计划项目（2018SF-155）。
作者简介：李慧敏(1981-)，女, 本科，主治医师，研究方向：产前诊断，E-mail：geliwu419@163.com。
通讯作者：杨琳(1982-)，男，本科，副主任医师。
更新日期/Last Update: 2022-09-15