[1]钟新丽,朱红霞,刘星娅,等.妊娠期糖尿病患者肝脏激酶B1 内含子380C ＞ T 基因多态性与糖脂代谢的关系及评估疾病易感性的价值[J].现代检验医学杂志,2022,37(05):55-60.[doi:10.3969/j.issn.1671-7414.2022.05.012]
　ZHONG Xin-li,ZHU Hong-xia,LIU Xing-ya,et al.Relationship between Liver Kinase B1 (LKB1) Intron 380C>T Gene Polymorphism and Glucose and Lipid Metabolism in Patients with Gestational Diabetes Mellitus (GDM) and Evaluate Value in Assessing Disease Susceptibility[J].Journal of Modern Laboratory Medicine,2022,37(05):55-60.[doi:10.3969/j.issn.1671-7414.2022.05.012]
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妊娠期糖尿病患者肝脏激酶B1 内含子380C ＞ T 基因多态性与糖脂代谢的关系及评估疾病易感性的价值()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第37卷
期数:
2022年05期
页码:
55-60
栏目:
论著
出版日期:
2022-09-15

文章信息/Info

Title:
Relationship between Liver Kinase B1 (LKB1) Intron 380C>T Gene Polymorphism and Glucose and Lipid Metabolism in Patients with Gestational Diabetes Mellitus (GDM) and Evaluate Value in Assessing Disease Susceptibility
文章编号:
1671-7414（2022）05-055-06
作者:
钟新丽; 朱红霞; 刘星娅; 曾凡英; 李 思; 李 英
成都市双流区第一人民医院，成都610200
Author(s):
ZHONG Xin-li;  ZHU Hong-xia;  LIU Xing-ya;  ZENG Fan-ying;  LI Si;  LI Ying
Chengdu Shuangliu District First People’s Hospital, Chengdu 610200, China
关键词:
妊娠期糖尿病; 肝脏激酶B1; 基因多态性; 糖脂代谢
分类号:
R714.256；Q786
DOI:
10.3969/j.issn.1671-7414.2022.05.012
文献标志码:
A
摘要:
目的　探讨妊娠期糖尿病（gestational diabetes mellitus, GDM）患者肝脏激酶B1（liver kinase B1, LKB1）内含子380C ＞ T 基因多态性与糖脂代谢的关系及评估疾病易感性的价值。方法　选取2019 年1 月～ 2021 年4 月成都市双流区第一人民医院收治的92 例GDM 患者作为GDM 组，依据1∶1 对照设计原则，另选同期92 例正常孕妇作为对照组。比较两组一般资料: 糖脂代谢指标[ 空腹血糖（fasting plasma glucose，FPG）、糖化血红蛋白（hemoglobin A1c，HbA1c）]、低密度脂蛋白胆固醇（low density lipoprotein cholesterol，LDL-C）、高密度脂蛋白胆固醇（high densitylipoprotein cholesterol，HDL-C）、三酰甘油（triglyceride，TG）和总胆固醇（total cholesterol，TC）等水平以及LKB1内含子380C ＞ T 基因多态性, 分析糖脂代谢指标水平与基因型的关系及GDM 易感性的相关影响因素。结果　GDM 组有GDM 家族史者占比（18/92, 19.57%）明显高于对照组（3/92, 3.26%），差异有统计学意义（χ2 =12.095，P ＜ 0.01）；GDM组FPG（6.14±0.67 mmol/L ），HbA1c（6.87%±0.31%），LDL-C（3.49±0.25 mmol/L），TG（2.31±0.54 mmol/L），TC（4.88±0.61 mmol/L）水平均明显高于对照组（4.52±0.33 mmol/L，5.09%±0.40%，3.05±0.27mmol/L，1.96±0.48mmol/L，4.39±0.72mmol/L）；HDL-C（1.06±0.19 mmol/L）水平明显低于对照组（1.33±0.22mmol/L），差异均有统计学意义（t=20.805，33.737，11.469，4.647，4.981 和8.909，均P ＜ 0.01）；GDM 组与对照组LKB1 内含子380C ＞ T 基因型中CT（40.22%），TT 基因型（18.48%）占比均明显高于对照组（27.17%，13.04%），CC 基因型（41.30%）明显低于对照组（59.78%），差异有统计学意义（χ2=6.292，P＜ 0.01）；LKB1内含子380C ＞T基因型TT患者HbA1c（7.13%±0.44%）水平高于基因型CT 患者（6.22%±0.39%），基因型CT 患者HbA1c 水平高于基因型CC 患者（5.46%±0.36%），差异有统计学意义（F=228.003，P ＜ 0.01）；且TT 和CT 基因型患者LDL-C 水平（3.59±0.37 mmol/L，3.45±0.32mmol/L）均明显高于CC 型患者LDL-C 水平（3.05±0.29mmol/L），差异有统计学意义（F=48.151，P ＜ 0.01）；HbA1c 与基因型呈强正相关，LDL-C 与基因型呈弱正相关（r=0.815，0.366，均P ＜ 0.01）；有GDM 家族史及LKB1 内含子380C＞ T 基因型TT 均为GDM 易感性的独立危险因素（P ＜ 0.01）。结论　LKB1 内含子380C ＞ T 基因突变可引起机体发生糖脂代谢紊乱，从而增高GDM 易感性，是GDM 发生的危险因素之一。
Abstract:
Objective　To investigate the relationship between liver kinase B1 (LKB1) intron 380C > T gene polymorphism and glycolipid metabolism in patients with gestational diabetes mellitus (GDM) and assess the susceptibility of disease. Methods　A total of 92 GDM patients admitted to the Chengdu Shuangliu District First People’s Hospital from January 2019 to April 2021 were selected as the GDM group. Based on the 1:1 control design principle, 92 normal pregnant women during the same period were selected as the control group. Compared the general information of the two groups: indicators of glucose metabolism [fasting plasma glucose (FPG), hemoglobin A1c (HbA1c)], low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (high density lipoprotein cholesterol, HDL-C), triglyceride (TG) and total cholesterol (TC), and LKB1 intron 380C>T gene polymorphism. Analysed the relationship of glucose metabolism index levels and genotype, and related influencing factors of GDM susceptibility. Results　The proportion of people with a family history of GDM in the GDM group (18/92, 19.57%) was significantly higher than that in the control group（3/92, 3.26%）, and the difference was statistically significant (χ2=12.095, P ＜ 0.01). The levels of FPG in the GDM group (6.14±0.67 mmol/L), HbA1c (6.87%±0.31%), LDL-C (3.49±0.25 mmol/L), TG (2.31±0.54 mmol/L) and TC (4.88±0.61 mmol/L) were significantly higher than the control group （4.52±0.33 mmol/L，5.09%±0.40%，3.05±0.27mmol/L，1.96±0.48mmol/L，4.39±0.72mmol/L）, and the level of HDL-C (1.06±0.19 mmol/L) was significantly lower than the control group（1.33±0.22mmol/L）,all the differences were statistically significant significance (t=20.805, 33.737, 11.469, 4.647, 4.981 and 8.909, all P ＜ 0.01). GDM group and control group LKB1 intron 380C>T genotype CT (40.22% ) and TT genotype ( 18.48% ) were significantly higher than the control group （27.17%，13.04%）, CC genotype(41.30%) were lower than the control group(59.78%), the difference was statistically significant (χ2=6.292, P ＜ 0.01).In LKB1 intron 380C>T genotype TT patients , the level of HbA1c (7.13%±0.44% ）was higher than that of genotype CT patients（6.22%±0.39 %), and the level of HbA1c in genotype CT patients was higher than that of CC patients（5.46%±0.36%), the difference was statistically significant (F=228.003, P ＜ 0.01). The levels of LDL-C (3.59±0.37 mmol/L and 3.45±0.32 mmol/L) in patients with TT and CT genotypes were significantly higher than that of patients with CC type（3.05±0.29 mmol/L), and the difference was both statistical significance (F=48.151, P ＜ 0.01). HbA1c was strongly positively correlated with genotype, and LDL-C was weakly positively correlated with genotype (r=0.815，0.366, all P ＜ 0.01). GDM with a family history and LKB1 intron 380C>T genotype TT could be independent risk factors for GDM susceptibility (P ＜ 0.01). Conclusion　The mutation of LKB1 intron 380C>T gene can cause disorders of glucose and lipid metabolism in the body, with increasing the susceptibility to GDM.It is one of the risk factors for the occurrence of GDM.

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备注/Memo

备注/Memo:
基金项目： 2017 年成都市医学科研课题计划，资助单位：成都市双流区第一人民医院，课题编号：2017046: 钠葡萄糖协同转运蛋白-2 与妊娠期糖尿病相关性研究。
作者简介：钟新丽（1972-），女，硕士研究生，副主任医师，研究方向：妊娠并发症，E-mail:738643692@qq.com。
更新日期/Last Update: 2022-09-15