[1]周 倩,陈 诚,陈智龙.急性冠脉综合征患者血清miR-361-5p 和COMP 表达水平及其与短期预后的相关性研究[J].现代检验医学杂志,2022,37(05):105-111+127.[doi:10.3969/j.issn.1671-7414.2022.05.021]
　ZHOU Qian,CHEN Cheng,CHEN Zhi-long.Study of Serum miR-361-5p and COMP Expression Levels in Patients with Acute Coronary Syndrome and Their Correlation with Short-term Prognosis[J].Journal of Modern Laboratory Medicine,2022,37(05):105-111+127.[doi:10.3969/j.issn.1671-7414.2022.05.021]
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急性冠脉综合征患者血清miR-361-5p 和COMP 表达水平及其与短期预后的相关性研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第37卷
期数:
2022年05期
页码:
105-111+127
栏目:
论著
出版日期:
2022-09-15

文章信息/Info

Title:
Study of Serum miR-361-5p and COMP Expression Levels in Patients with Acute Coronary Syndrome and Their Correlation with Short-term Prognosis
文章编号:
1671-7414（2022）05-105-08
作者:
周 倩1;  陈 诚1;  陈智龙2
1. 鄂东医疗集团黄石市中心医院/ 湖北理工学院附属医院老年病科，湖北黄石 435002；2. 黄石市中医院心血管内科，湖北黄石 435099
Author(s):
ZHOU Qian1 ; CHEN Cheng1 ; CHEN Zhi-long2
1.Department of Geriatrics，Huangshi Central Hospital of Edong Healthcare Group / Affiliated Hospital of Hubei Polytechnic University, Hubei Huangshi 435002,China; 2.Department of Cardiology, Huangshi Hospital of Traditional Chinese Medicine，Hubei Huangshi 435099,China
关键词:
急性冠脉综合征; 微小核糖核酸-361-5p; 软骨寡聚基质蛋白
分类号:
R541.4；R392.11
DOI:
10.3969/j.issn.1671-7414.2022.05.021
文献标志码:
A
摘要:
目的　研究急性冠脉综合征（acute coronary syndrome ，ACS） 患者血清微小核糖核酸（micro RNA ，miRNA）-361-5p 和软骨寡聚基质蛋白（cartilage oligomeric matrix protein ，COMP）表达水平及其与短期预后的相关性。方法　选取2020 年1 月～ 2021 年1 月鄂东医疗集团黄石市中心医院收治的170 例ACS 患者为ACS 组，根据Gensini积分分为重度狭窄组（n=41）、中度狭窄组（n=78）和轻度狭窄组（n=51）。另选取同期66 例体检健康者为对照组。qRT-PCR 与ELISA 检测血清miR-361-5p 和COMP 水平；Pearson 相关性分析ACS 患者血清miR-361-5p 和COMP 水平与Gensini 评分的相关性；多因素Logistic 回归分析ACS 患者短期预后不良影响因素，受试者工作特征（ROC）曲线分析血清miR-361-5p 和COMP 水平对ACS 发病和短期预后的预测价值。结果　与对照组比较，ACS 组血清miR-361-5p（0.58±0.21 vs 0.33±0.18）和COMP（143.64±25.23ng/ml vs 112.35±19.09ng/ml）水平均升高，差异有统计学意义（t=8.625，10.277，均P ＜ 0.001）。轻度狭窄组、中度狭窄组和重度狭窄组血清miR-361-5p(0.34±0.11，0.63±0.15， 0.76±0.12)和COMP[118.01（108.29, 125.52）ng/ml，147.71（134.72, 158.63）ng/ml，162.32（158.74, 177.71）ng/ml] 水平依次升高，差异有统计学意义（F=131.941，H=101.001，均P ＜ 0.001）。Pearson 相关性分析显示，ACS 患者血清miR-361-5p和COMP 水平与Gensini 评分呈正相关（r=0.765，0.755，均P ＜ 0.001）。随访3 个月，170 例ACS 患者主要不良心血管事件发生率为13.53%（23/170），多因素Logistic 回归分析显示，miR-361-5p（OR=1.524，95%CI：1.240 ～ 2.023，P=0.004）、COMP（OR=1.646，95%CI：1.318 ～ 2.075，P=0.001）是ACS 患者短期预后不良的独立影响因素。ROC曲线显示，miR-361-5p 预测ACS 发病和短期预后不良的曲线下面积（AUC）为0.813 和0.776，COMP 预测ACS 发病和短期预后不良的AUC 为0.836 和0.782，miR-361-5p ＋ COMP 联合预测ACS 发病和短期预后不良的AUC 为0.904和0.863。miR-361-5p ＋ COMP 联合预测ACS 发病和短期预后不良的AUC 大于miR-361-5p 和COMP 单独预测，差异均有统计学意义（Z=4.684，4.573；2.494，2.617，均P ＜ 0.05）。结论　ACS 患者血清miR-361-5p 和COMP 水平升高，与冠状动脉狭窄程度密切相关，可作为ACS 发病和短期预后不良的预测指标。
Abstract:
Objective　To study the expression levels of serum micro RNA (miRNA)-361-5p and cartilage oligomeric matrix protein (COMP) in patients with acute coronary syndrome (ACS) and their correlation with short-term prognosis.Methods　A total of 170 ACS patients admitted to Huangshi Central Hospital of Edong Healthcare Group from January 2020 to January 2021 were selected as ACS group.According to Gensini scores, they were divided into severe stenosis group (n=41) and moderate stenosis group (n=78), mild stenosis group (n=51), and 66 healthy people who had physical examination during the same period were selected as the control group. Serum miR-361-5p and COMP levels were measured by qRT-PCR with ELISA.Pearson correlation was used to analyze the correlation between serum miR-361-5p and COMP levels and Gensini score in ACS patients. Analysis of factors influencing poor prognosis in patients with ACS by multifactorial logistic regression.The predictive value of serum miR-361-5p and COMP levels on the onset and short-term prognosis of ACS was analyzed by plotting receiver operating characteristic (ROC) curves. Results　Serum miR-361-5p (0.58±0.21 vs 0.33±0.18) and COMP (143.64±25.23 ng/ ml vs 112.35±19.09 ng/ml) levels were increased in the ACS group compared with the control group, with statistically significant differences (t=8.625，10.277, all P ＜ 0.001). Serum miR-361-5p (0.34±0.11，0.63±0.15 ，0.76±0.12) and COMP [118.01(108.29,125.52) ng/ml，147.71(134.72,158.63) ng/ml，162.32(158.74,177.71) ng/ml] levels were sequentially increased in the mild, moderate and severe stenosis groups, with statistically significant differences (F=131.941, H=101.001, all P ＜ 0.001). Pearson correlation analysis showed that serum miR-361-5p and COMP levels were positively correlated with Gensini score in ACS patients (r=0.765，0.755, all P ＜ 0.001).At 3-month follow-up, the incidence of major adverse cardiovascular events was 13.53% (23/170) in 170 patients with ACS, and multifactor logistic regression analysis showed that miR-361-5p (OR=1.524, 95% CI: 1.240 ～ 2.023, P=0.004) and COMP (OR=1.646, 95% CI: 1.318 ～ 2.075, P=0.001) were independent influences on the poor short-term prognosis of ACS patients.The ROC curves showed that the area under the curve (AUC) for miR-361-5p predicting ACS onset and short-term poor prognosis were 0.813 and 0.776 respectively, the AUC for COMP predicting ACS onset and short-term poor prognosis were 0.836 and 0.782 respectively, and the AUC for miR-361-5p ＋ COMP combined predicting ACS onset and short-term poor prognosis were 0.904 and 0.863 respectively.The combined AUC of miR- 361-5p ＋ COMP predicted greater ACS onset and short-term poor prognosis than miR-361-5p and COMP alone, with statistically significant differences (Z=4.684, 4.573; 2.494, 2.617, all P＜ 0.05). Conclusion　Elevated serum miR-361-5p and COMP levels in ACS patients were closely associated with the degree of coronary stenosis and can be used as predictors of ACS onset and poor short-term prognosis.

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备注/Memo

备注/Memo:
基金项目：湖北省卫健委科研基金 （WJ2019H491）。
作者简介：周倩（1986-），女，硕士，主治医师，研究方向：老年病，E-mail：zhouqian6678@163.com。
通讯作者：陈诚(1985-)，男，硕士， 主治医师, 研究方向：老年病，E-mail：chencheng990a@163.com。
更新日期/Last Update: 2022-09-15