[1]王 勇,张克波.老年心力衰竭患者血清NF-κB，Gal-3 和sST2 水平联合检测与预后评估价值[J].现代检验医学杂志,2022,37(05):148-153.[doi:10.3969/j.issn.1671-7414.2022.05.029]
　WANG Yong,ZHANG Ke-bo.Prognostic Value of Combined Serum NF-κB, Gal-3 and sST2 Levels in Elderly Patients with Heart Failure[J].Journal of Modern Laboratory Medicine,2022,37(05):148-153.[doi:10.3969/j.issn.1671-7414.2022.05.029]
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老年心力衰竭患者血清NF-κB，Gal-3 和sST2 水平联合检测与预后评估价值()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第37卷
期数:
2022年05期
页码:
148-153
栏目:
论著
出版日期:
2022-09-15

文章信息/Info

Title:
Prognostic Value of Combined Serum NF-κB, Gal-3 and sST2 Levels in Elderly Patients with Heart Failure
文章编号:
1671-7414（2022）05-148-06
作者:
王 勇; 张克波
广元市精神卫生中心检验科，四川广元 628000
Author(s):
WANG Yong;  ZHANG Ke-bo
Department of Clinical Laboratory，Guangyuan Mental Health Center， Sichuan Guangyuan 628000，China
关键词:
老年心力衰竭; 核因子-κΒ; 半乳糖凝集素3; 可溶性生长刺激表达基因2 蛋白
分类号:
R541.6；R392.11
DOI:
10.3969/j.issn.1671-7414.2022.05.029
文献标志码:
A
摘要:
目的　探讨老年心力衰竭（heart failure，HF）患者血清核因子-κΒ（nuclear factor-κΒ，NF-κΒ）、半乳糖凝集素3（galectin 3，Gal-3）和可溶性生长刺激表达基因2（soluble growth-stimulating expression gene 2，sST2）蛋白水平联合检测与预后评估价值。方法　选取广元市精神卫生中心2018 年1 月～ 2019 年12 月收治的100 例HF 患者为HF组，随访一年根据预后情况分为预后不良组（n=52）和预后良好组（n=48），另选取同期60 例体检健康者为对照组。酶联免疫吸附法（enzyme-linked immunosorbent assay，ELISA）检测血清NF-κΒ，Gal-3 和sST2 水平。比较HF 组和对照组血清NF-κΒ，Gal-3 和sST2 水平，并比较不同纽约心脏协会（New York Heart Association Classification，NYHA）心功能分级HF 组血清NF-κΒ，Gal-3 和sST2 水平。多因素Logistic 回归分析老年HF 患者预后不良影响因素，ROC曲线分析血清NF-κΒ，Gal-3 和sST2 水平对老年HF 患者预后的评估价值。结果　HF 组血清NF-κΒ，Gal-3 和sST2水平（25.15±6.37pg/ml，104.96±26.11ng/L，1 117.80±494.96pg/ml）高于对照组（16.56±4.14pg/ml，74.71±15.05ng/L，584.00±191.50pg/ml），差异均有统计学意义（t=9.321，8.189，7.994，均P ＜ 0.001）。Ⅲ～Ⅳ级老年HF 患者血清NF-κΒ，Gal-3 和sST2 水平（28.30±5.71pg/ml，113.75±25.24ng/L，1 346.99±468.64pg/ml）高于Ⅰ～Ⅱ级（22.57±5.73pg/ml，97.77±24.78ng/L，930.29±436.59pg/ml），差异均有统计学意义（t=-4.983，-3.182，-4.594，均P ＜ 0.001）。随访一年，100 例HF 患者预后不良52 例。多因素Logistic 回归分析显示，NYHA 心功能分级Ⅲ～Ⅳ级、高血清氨基末端脑钠肽前体（N terminal pro B type natriuretic peptide，NT-proBNP），NF-κΒ，Gal-3 和sST2 水平为老年HF 患者预后不良独立危险因素（OR=1.003 ～ 8.653，均P ＜ 0.05）。ROC 曲线显示，NF-κΒ，Gal-3，sST2 和NF-κΒ+Gal-3+sST2评估老年HF 患者不良预后的曲线下面积分别为0.804，0.747，0.776 和0.882，NF-κΒ+Gal-3+sST2 评估老年HF 患者不良预后的曲线下面积大于各指标单独预测（Z=2.173 ～ 3.177，均P ＜ 0.05）。结论　老年HF 患者血清NF-κΒ，Gal-3 和sST2 水平明显提升，与心功能变化和预后相关，联合检测可提升老年HF 患者预后评估价值。
Abstract:
Objective　To investigate the value of combined serum nuclear factor-κΒ (NF-κΒ), galectin 3 (Gal-3) and soluble growth-stimulating expression gene 2 (sST2) protein levels in elderly patients with heart failure (HF) and prognostic assessment. Methods　A total of 100 HF patients admitted to Guangyuan Mental Health Center (January 2018 to December 2019) were selected as the HF group, and they were divided into a poor prognosis group (n=52) and a good prognosis group(n=48) according to the prognosis for 1 year，and another 60 healthy persons undergoing physical examination during the same period were selected as control group. Serum NF-κΒ，Gal-3 and sST2 levels were measured by enzyme-linked immunosorbent assay （ELISA）. Serum NF-κΒ, Gal-3 and sST2 levels were compared between the HF and control groups, and serum NF-κΒ, Gal-3 and sST2 levels were compared between different New York Heart Association (NYHA) cardiac function classes in the HF group. Multi-factor logistic regression was used to analyze factors influencing poor prognosis in elderly HF patients, and ROC curves were used to analyze the value of serum elderly NF-κΒ, Gal-3 and sST2 levels in assessing the prognosis of elderly HF patients. Results　The serum NF-κB, Gal-3 and sST2 levels in the HF group(25.15±6.37 pg/ml, 104.96±26.11ng/L，1 117.80±494.96pg/ml ) were significantly higher than those in the control group (16.56±4.14 pg/ml, 74.71±15.05ng/L, 584.00±191.50pg/ml) and the differences were statistically significant（t=9.321，8.189，7.994，all P ＜ 0.001）. The serum NF-κB, Gal-3 and sST2 levels of elderly HF patients of grade Ⅲ ～ Ⅳ (28.30±5.71pg/ml, 113.75±25.24ng/L, 1 346.99±468.64pg/ml) were significantly higher than those of grade Ⅰ ~ Ⅱ (22.57±5.73 pg/ml, 97.77±24.78ng/L, 930.29±436.59 pg/ml), and the differences were statistically significant （t=-4.983，-3.182，-4.594，all P ＜ 0.001）. At one year follow-up, 52 of 100 HF patients had poor prognosis. Multi-factorial logistic regression analysis showed that NYHA cardiac function class III~IV, high serum amino-terminal pro B type natriuretic peptide (NT-proBNP), NF- κΒ, Gal-3 and sST2 levels were independent risk factors for poor prognosis in elderly HF patients (OR=1.003 ～ 8.653, all P ＜ 0.05). The ROC curves showed that the area under the curve for NF-κΒ, Gal-3, sST2 and NF-κΒ+Gal-3+sST2 to assess poor prognosis in elderly HF patients was 0.804, 0.747, 0.776 and 0.882, respectively, and the area under the curve for NF- κΒ+Gal-3+sST2 to assess poor prognosis in elderly HF patients was greater than that predicted by each index alone (Z=2.173 ～ 3.177, all P ＜ 0.05). Conclusion　Serum NF-κΒ, Gal-3 and sST2 levels were significantly elevated in elderly HF patients and correlate with changes in cardiac function and prognosis. The combined test may enhance the prognostic assessment value of elderly HF patients.

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备注/Memo

备注/Memo:
基金项目：保定市科技局社发类项目：高龄心力衰竭患者常见合并症及其干预措施的研究（编号：1941ZF029）。
作者简介：王勇（1975-），男，本科，副主任检验师，研究方向：医学检验，E-mail：hello200009@126.com。
更新日期/Last Update: 2022-09-15