[1]樊娜娜,韩 清,鲁 静,等.血清1- 硬脂酰-sn- 甘油-3- 磷酰胆碱水平检测对妊娠期糖尿病的诊断价值[J].现代检验医学杂志,2022,37(06):114-118.[doi:10.3969/j.issn.1671-7414.2022.06.021]
 FAN Na-na,HAN Qing,LU Jing,et al.Diagnostic Value of Serum 1-Stearoyl-sn-Glycero-3-Phosphorylcholine Level in Gestational Diabetes Mellitus[J].Journal of Modern Laboratory Medicine,2022,37(06):114-118.[doi:10.3969/j.issn.1671-7414.2022.06.021]
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血清1- 硬脂酰-sn- 甘油-3- 磷酰胆碱水平检测对妊娠期糖尿病的诊断价值()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第37卷
期数:
2022年06期
页码:
114-118
栏目:
论著
出版日期:
2022-11-15

文章信息/Info

Title:
Diagnostic Value of Serum 1-Stearoyl-sn-Glycero-3-Phosphorylcholine Level in Gestational Diabetes Mellitus
文章编号:
1671-7414(2022)06-114-05
作者:
樊娜娜1韩 清1鲁 静1徐 文2
(1. 枣庄市妇幼保健院产科, 山东枣庄 277000;2. 青岛大学附属医院药学部, 青岛 266003)
Author(s):
FAN Na-na1 HAN Qing1 LU Jing1 XU Wen2
(1. Department of Obstetrics,Maternal and Child Health Care Hospital of Zaozhuang, Shandong Zaozhuang 277000 China; 2. Department of Pharmacy, the Affiliated Hospital of Qingdao University, Shandong Qingdao 266003, China)
关键词:
1- 硬脂酰-sn- 甘油-3- 磷酰胆碱妊娠期糖尿病液相色谱- 串联质谱
分类号:
R714.256;R446.11
DOI:
10.3969/j.issn.1671-7414.2022.06.021
文献标志码:
A
摘要:
目的 探讨血清1- 硬脂酰-sn- 甘油-3- 磷酰胆碱(1-stearoyl-sn-glycero-3-phosphorylcholine, LPC18:0)水平检测对妊娠期糖尿病(gestational diabetes mellitus, GDM)的诊断价值。方法 选择2020 年5 ~ 12 月在枣庄市妇幼保健院进行产前检查的孕妇60 例为研究对象,根据口服葡萄糖耐量试验(oral glucose tolerance test,OGTT) 测定结果将妊娠妇女分为GDM 孕妇(观察组)30 例和糖耐量正常孕妇(对照组)30 例。利用液相色谱- 串联质谱(liquidchromatography-tandem mass spectrometry, LC-MS/MS)的代谢组学技术检测两组孕妇血清 LPC18:0 表达水平,分析GDM 孕妇血清LPC18:0 水平与临床、糖脂指标的相关性,利用受试者工作特征曲线(receiver operating characteristiccurve,ROC)评价血清中LPC18:0 诊断GDM 的价值。结果 观察组LPC 18:0[100.42(76.80,142.08)μg/ml]、总胆固醇(TC)(3.52 ± 0.51 mmol/L )、空腹血糖(FPG)(5.31 ± 2.12 mmol/L )、低密度脂蛋白- 胆固醇(LDL-C)(3.81± 0.98 mmol/L)、空腹胰岛素(FINS)(16.65 ± 6.78μIU/ml)、胰岛素抵抗指数(HOMA-IR)(3.32 ± 1.08)和糖化血红蛋白(HbA1c)(5.61 %± 0.31 %)水平均高于对照组[30.88(22.08,40.60) μg/ml,3.12 ± 0.68 mmol/L,5.01 ± 1.47 mmol/L,3.42 ± 0.52 mmol/L, 10.98 ± 4.89 μIU/ml,2.27 ± 0.99 ,5.01 %± 0.50 %],差异有统计学意义(z=-5.28,t=2.48 2.83,3.01,3.43,4.43,5.34,均P < 0.05)。观察组和对照组孕妇三酰甘油(TG)(2.11 ± 0.47mmol/L vs 1.91 ± 0.82 mmol/L),高密度脂蛋白- 胆固醇(HDL-C)(1.98 ± 0.38 mmol/L vs 2.01 ± 0.56 mmol/L)水平比较,差异均无统计学意义(t=0.66,-0.67,均P > 0. 05)。GDM 孕妇血清LPC18:0 水平与孕妇年龄、孕前体重指数,TG,HDL-C,HbA1c 水平无相关性(r=-0.14 ~ 0.17,均P > 0.05),与FPG,TC,LDL-C 水平呈正相关(r=0.28,0.41,0.46,均P < 0.05),与FINS,HOMA-IR 呈负相关(r= -0.33,-0.51,均P < 0.05)。ROC 曲线分析结果显示,区别诊断GDM孕妇及正常孕妇时,血清LPC18:0 的曲线下面积(area under curve,AUC)为 0.988(95%CI: 0.964 ~ 1.000),当最佳临界值62.25 μg/ml 时,特异度和灵敏度分别为95% 和90%。结论 利用LC-MS/MS 方法检测GDM 孕妇血清LPC18:0 水平较正常孕妇明显升高,血清LPC18:0 水平检测对GDM 有一定的诊断价值。
Abstract:
Objective To investigate the diagnostic value of serum 1-stearoyl-sn-glycero-3-phosphorylcholine (LPC18:0) level in gestational diabetes mellitus (GDM). Methods A total of 60 pregnant women who underwent prenatal examination in Maternal and Child Health Hospital of Zaozhuang from May to December 2020 were selected as the study subjects. According to oral glucose tolerance test(OGTT) measurement results, pregnant women were divided into GDM pregnant women (observation group) 30 cases, normal glucose tolerance pregnant women(control group) 30 cases. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to detect the expression of LPC18:0 in serum of two groups of pregnant women. The correlation between serum LPC18:0 level and clinical and glucolipid indexes in pregnant women with GDM was analyzed, and the diagnostic value of serum LPC18:0 in GDM was evaluated by receiver operating characteristic curve (ROC). Results LPC18:0[100.42 (76.80, 142.08)μg/ml], total cholesterol (TC) (3.52 ± 0.51 mmol/L), fasting glucose (FPG) (5.31 ± 2.12 mmol/L), low density lipoprotein -cholesterol(LDL-C) (3.81 ± 0.98 mmol/L), fasting insulin (FINS) (16.65 ± 6.78 μIU/ ml), the levels of insulin resistance index (HOMA-IR) (3.32 ± 1.08 ) and HbA1c (5.61%±0.31%) were higher than those of the control group[30.88(22.08,40.60) μg/ml,3.12 ± 0.68 mmol/L,5.01 ± 1.47 mmol/L,3.42 ± 0.52 mmol/L, 10.98 ± 4.89 μIU/ml,2.27 ± 0.99 ,5.01 %± 0.50 %],the differences were statistical significant (z=-5.28,t=2.48,2.83,3.01,3.43, 4.43,5.34,all P<0.05). Comparison of triglyceride (TG)(2.11 ± 0.47 mmol/L vs 1.91 ± 0.82 mmol/L)and high-density lipoprotein-cholesterol (HDL-C) (1.98 ± 0.38 mmol/L vs 2.01 ± 0.56 mmol/L) levels between observation group and control group, there differences were no statistically significant(t=0.66, -0.67, all P > 0.05). Spearman correlation analysis showed that there was no correlation between serum LPC18.0 level and pregnant women’s age, prepregnancy body mass index, TG, HDL-C and HbA1c (r=-0.14 ~ 0.17,all P>0.05),and positively correlated with FPG, TC and LDL-C (r=0.28,0.41,0.46,all P< 0.05), and negatively correlated with FINS and HOMA-IR (r=-0.33,-0.51, all P < 0.05). ROC curve analysis showed that the area under curve (AUC) of serum LPC18.0 in GDM pregnant women was 0.988(95%CI: 0.964 ~ 1.000). When the optimal critical value was 62.25 μg/ml,the specificity and sensitivity were 95% and 90%, respectively. Conclusion The level of serum LPC18:0 in pregnant women with GDM detected by LC-MS/MS was significantly higher than that in normal pregnant women, and the detection of serum LPC18:0 has certain diagnostic value for GDM.

参考文献/References:

[1] 谢幸 , 苟文丽 . 妇产科学 [M]. 北京 : 人民卫生出版社 , 2019: 64-66. XIE Xing, GOU Wenli. Obstetrics and Gynecology[M]. Beijing: People’s Medical Publishing House, 2019: 64-66.
[2] 张小平 , 余海珍 , 赵家宁 , 等 . 孕妇妊娠中期糖脂代谢紊乱对不良妊娠结局的影响 [J]. 现代检验医学杂志 , 2015, 30(6): 35-38. ZHANG Xiaoping, YU Haizhen , ZHAO Jianing, et al. Impaction of glucose and lipids metabolic abnormalities of pregnant women in second trimester for adverse pregnancy outcomes [J]. Journal of Modern Laboratory Medicine. 2015, 30(6): 35-38.
[3] LIU Jinnan, LI Jing, LI Sainan, et al. Circulating Lysophosphatidylcholines in early pregnancy and risk of gestational diabetes in Chinese women[J]. J Clin Endocrinol Metab, 2020, 105(4): dgaa058.
[4] 樊娜娜 , 詹瑛 , 徐文 , 等 .子痫前期患者血清代谢物水平及其与疾病严重程度的相关性研究 [J].国际检验医学杂志 ,2021,42(5):568-573. FAN Nana, ZHAN Ying, XU Wen. et, al. Study on serum metabolite level and its correlation with disease severity in patients with preeclampsia [J]. International Journal of Laboratory Medicine, 2021,42(5):568-573.
[5] 任丽芬 , 张志平 , 徐蓓 , 等 .孕妇葡萄糖耐量和血清胰岛素及 C-肽释放水平检测对妊娠糖尿病的诊断研究 [J].现代检验医学杂志 , 2020, 35(6):167-170. REN Lifen, ZHANG Zhiping, XU Bei, et al. Study on diagnosis of gestational diabetes mellitus by detection of glucose tolerance,serum insulin and C-peptide release levels in pregnant women [J]. Journal of Modern Laboratory Medicine, 2020, 35(6):167-170.
[6] 吴小绸 , 吴克亮 , 孙晓峰 , 等 .在不同妊娠阶段妊娠期糖尿病孕妇的血清代谢组学的分析 [J].中国临床药理学杂志 , 2019, 35(22):2823-2826. WU Xiaochou, WU Keliang, SUN Xiaofeng. et al. Serum metabolomics analysis of pregnant women with gestational diabetes mellitus in different gestational stages [J]. The Chinese Journal of Clinical Pharmacology, 2019, 35(22): 2823-2826.
[7] LAW S H, CHAN M L, MARATHE G K, et al. An updated review of Lysophosphatidylcholine metabolism in human diseases [J]. Int J Mol Sci, 2019, 20(5):1149.
[8] FURSE S, WHITE S L, MEEK C L, et, al. Altered triglyceride and phospholipid metabolism predates the diagnosis of gestational diabetes in obese pregnancy [J]. Mol Omics. 2019, 15(6):420-430.
[9] LIU Panpan, ZHU Wu, CHEN Chao, et, al. The mechanisms of lysophosphatidylcholine in the development of diseases [J]. Life Sci, 2020, 247: 117443.
[10] YEA K, KIM J, YOON J H, et, al. Lysophosphatidyl-choline activates adipocyte glucose uptake and lowers blood glucose levels in murine models of diabetes [J]. J Biol Chem. 2009, 284(49):33833-40.
[11] DRZAZGA A, OKULUS M, RYCHLICKA M, et al. Lysophosphatidylcholine containing anisic acid is able to stimulate insulin secretion targeting g protein coupled receptors[J]. Nutrients, 2020, 12(4):1173.
[12] DRZAZGA A, KAMI?SKA D, GLISZCZY?SKA A, et al. Isoprenoid derivatives of Lysophosphatidylcholines enhance insulin and GLP-1 secretion through lipid-binding GPCRs[J]. Int J Mol Sci, 2021, 22(11):5748.
[13] TAKAHASHI H, GOTO T, YAMAZAKI Y, et al. Me-tabolomics reveal 1-palmitoyl Lysophosphatidylcholine production by peroxisome proliferator-activated recep-tor α[J]. J Lipid Res, 2015, 56(2):254-265.
[14] WANG Jiayue, WANG Bohong, ZHANG Yan. Agonism activities of Lysophosphatidylcholines (LPC) ligands binding to peroxisome proliferator-activated receptor gamma (PPARγ) [J]. J Biomol Struct Dyn, 2020, 38(2):398-409.
[15] ZHONG Huanzi, FANG Chao, FAN Yanqun, et al.Lipidomic profiling reveals distinct differences in plasma lipid composition in healthy, prediabetic, and type 2 diabetic individuals[J].Gigascience, 2017, 6(7):1-12.

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备注/Memo

备注/Memo:
作者简介:樊娜娜(1984-),女,硕士,主治医师,研究方向:围产医学与代谢性疾病的研究,E-mail:fannana1984521@163.com。
通讯作者:韩清(1979-),女,本科,主管护师, 研究方向:围产医学及产科护理研究,E-mail:zbdu2005@163.com。
更新日期/Last Update: 2022-11-15