[1]温小英,乔印玲,邓桂林.输卵管妊娠患者血清IL-33 和sST2 表达水平与输卵管妊娠期待治疗疗效的相关性研究[J].现代检验医学杂志,2022,37(06):129-133+139.[doi:10.3969/j.issn.1671-7414.2022.06.024]
　WEN Xiao-ying,QIAO Yin-ling,DENG Gui-lin.Correlation between the Expression Levels of Serum IL-33 and sST2 in Patients with Tubal Pregnancy and the Efficacy of Expectant Treatment for Tubal Pregnancy[J].Journal of Modern Laboratory Medicine,2022,37(06):129-133+139.[doi:10.3969/j.issn.1671-7414.2022.06.024]
点击复制

输卵管妊娠患者血清IL-33 和sST2 表达水平与输卵管妊娠期待治疗疗效的相关性研究()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第37卷
期数:
2022年06期
页码:
129-133+139
栏目:
论著
出版日期:
2022-11-15

文章信息/Info

Title:
Correlation between the Expression Levels of Serum IL-33 and sST2 in Patients with Tubal Pregnancy and the Efficacy of Expectant Treatment for Tubal Pregnancy
文章编号:
1671-7414(2022)06-129-06
作者:
温小英; 乔印玲; 邓桂林
(贵州航天医院妇科，贵州遵义563000)
Author(s):
WEN Xiao-ying;  QIAO Yin-ling;  DENG Gui-lin
(Department of Gynecology, Guizhou Aerospace Hospital, Guizhou Zunyi 563000, China)
关键词:
白细胞介素-33; 可溶性ST2; 输卵管妊娠; 期待治疗
分类号:
R714.221；R392.11
DOI:
10.3969/j.issn.1671-7414.2022.06.024
文献标志码:
A
摘要:
目的 探讨白细胞介素(interleukin-33，IL)-33，可溶性ST2(soluble ST2，sST2)与输卵管妊娠期待治疗疗效关系及预测价值。方法 选取2019 年4 月～ 2021 年4 月贵州航天医院137 例输卵管妊娠患者，根据期待治疗是否成功分为成功组、未成功组。采用电化学发光法(electrochemiluminescence, ECL)检测治疗前、期待治疗7 天后血清IL-33和sST2 水平，应用放射免疫法(radioimmunoassay, RIA)检测治疗前、期待治疗7 天后血清人绒毛膜促性腺激素(humanchorionic gonadotropin，hCG)水平，分析IL-33，sST2 与血清hCG 相关性及期待治疗疗效的相关影响因素，并分析IL-33，sST2 及联合预测期待治疗成功的价值，并比较不同IL-33，sST2 表达水平患者期待治疗成功率。结果 全组患者期待治疗中，92 例(67.15%)患者成功，45 例(32.85%)患者未成功；未成功组治疗前IL-33(110.97±36.05ng/ml)低于成功组(168.59±42.37ng/ml)，sST2(31.52±5.83ng/ml)高于成功组(25.04±4.19ng/ml)，差异具有统计学意义(t=7.837，7.442， 均P ＜ 0.05)； 治疗后成功组IL-33(180.35±24.61ng/ml) 高于治疗前(168.59±42.37ng/ml)，sST2(19.52±5.27ng/ml)低于治疗前(25.04±4.19ng/ml)，差异具有统计学意义(t=2.302，7.864，均P ＜ 0.05)；治疗前、治疗后IL-33 与hCG 呈负相关(r=-0.867，-0.882，均P ＜ 0.001)，治疗前、治疗后sST2 与hCG 呈正相关(r=0.815，0.849，均P ＜ 0.001)；调整了临床表现、血清hCG 两次检测变化、子宫内膜厚度混杂因素后，治疗前IL-33，sST2 仍是发生期待治疗未成功的相关影响因素(均P ＜ 0.05)；IL-33，sST2 单一及两者联合预测输卵管妊娠的期待治疗成功的AUC 分别为0.849，0703，0.911；IL-33 高水平者成功率(91.89%)高于低水平者(38.10%)，sST2 高水平者成功率(55.79%)低于低水平者(92.86%)，差异均有统计学意义(χ2=44.648，18.142，均P ＜ 0.05)。结论 IL-33，sST2均与输卵管妊娠期待治疗成功情况有关。两者联合检测可作为预测期待治疗疗效的生物标志物，指导临床治疗决策。
Abstract:
Objective To explore the value of interleukin-33 (IL-33) and soluble ST2 (sST2) in predicting the success of expectant treatment of tubal pregnancy. Methods 137 patients with tubal pregnancy in Guizhou Aerospace Hospital from April 2019 to April 2021 were selected and divided into successful group and unsuccessful group according to whether they expected treatment to be successful or not. Serum IL-33, sST2 levels before treatment and after 7d of expectant treatment were detected by electrochemiluminescence(ECL), and serum human chorionic gonadotropin (hCG) levels before treatment and after 7 d of expectant treatment were detected by radioimmunoassay(RIA). The correlation between IL-33, sST2 and serum hCG, and the efficacy of expectant treatment were analyzed. The value of IL-33, sST2 and the combination to predict the success of prospective treatment were analyzed, and compared the success rate of prospective treatment in patients with different IL-33, sST2 expression levels. Results The whole group of patients were expecting treatment, 92 patients (67.15%) were successful and 45 patients (32.85%) were unsuccessful. IL-33 (110.97±36.05ng/ml) before treatment in the unsuccessful group was lower than that in the successful group (168.59±42.37ng/ml) and sST2 (31.52±5.83ng/ml) was higher than that in the successful group (25.04±4.19ng/ml), the differences were statistically significant (t=7.837, 7.442, all P ＜ 0.05).IL-33 (180.35±24.61ng/ml) was higher in the success group than before treatment (168.59±42.37ng/ml), and sST2 (19.52±5.27ng/ml) was lower than before treatment (25.04±4.19ng/ml)，the differences were statistically significant (t=2.302, 7.864, all P ＜ 0.05). IL-33 was negatively correlated with hCG before and after treatment (r=-0.867,-0.882, all P ＜ 0.001), and sST2 was positively correlated with hCG before and after treatment (r=0.815, 0.849, all P ＜ 0.001). After adjusting for clinical manifestations, changes in serum hCG twice tested, and endometrial thickness confounders, IL-33 and sST2 before treatment were still relevant influences for the occurrence of unsuccessful expectant treatment (all P ＜ 0.05). The AUCs for IL-33, sST2 and both combined to predict expectant treatment success in tubal pregnancy were 0.849, 0703 and 0.911. The success rates were higher in those with high levels of IL-33 (91.89%) than in those with low levels (38.10%) and lower in those with high levels of sST2 (55.79%) than in those with low levels (92.86%), with statistically significant differences (χ2=44.648, 18.142, all P ＜ 0.05). Conclusion Both IL-33 and sST2 were associated with the success of pending treatment during tubal pregnancy. Combined testing of the two can be used as a biomarker to predict the efficacy of prospective treatment and guide clinical treatment decisions.

参考文献/References:

[1] DOOLEY W, DE BRAUD L, MEMTSA M, et al. Physical resolution of tubal ectopic pregnancy on ultrasound imaging following successful expectant management[J]. Reproductive Biomedicine Online, 2020, 40(6): 880-886.
[2] 王豫红 , 朱兰 .异位妊娠期待治疗的进展 [J].中华医学杂志 , 2021, 101(7):524-526. WANG Yuhong ,ZHU LAN .Progress in expectant treatment of ectopic pregnancy [J]. National Medical Journal of China, 2021, 101(7): 524-526.
[3] 杨翠丽 , 张广美．输卵管妊娠治疗方式及其对未来生育影响的研究进展 [J].国际生殖健康 /计划生育杂志 , 2019, 38(1): 63-67. YANG Cuili, ZHANG Guangmei . Advances in the treatment of tubal pregnancy and its influence on future fertility[J]. Journal of International Reproductive Health/Family Planning,2019,38(1): 63-67.
[4] SABBIONI L,CAROSSINO E,SEVERI F M, et al. From β-hCG values to counseling in tubal pregnancy: what do women want[J]. Gynecological Endocrinology, 2019, 35(12): 1021-1026.
[5] LOGIODICE F, LOMBARDELLI L, KULLOLLI O A, et al. Decidual interleukin-22-producing CD4+T cells (Th17/Th0/IL-22+and Th17/Th2/IL-22+, Th2/IL-22+, Th0/IL-22+), which also produce IL-4, are involved in the success of pregnancy[J]. International Journal of Molecular Sciences, 2019, 20(2): 428.
[6] 胡蝶 , 浦春 .可溶性 ST2及其配体 IL-33与疾病关系的研究进展 [J].临床输血与检验 , 2021, 23(1):131-134. HU Die, PU Chun. Research progress on the relationship between soluble ST2 and its ligand IL-33 and disease[J]. Journal of Clinical Transfusion and Laboratory Medicine,2021,23 (1): 131-134
[7] 王玉东 , 陆琦．输卵管妊娠诊治的中国专家共识 [J].中国实用妇科与产科杂志 , 2019, 35(7): 780-787. WANG Yudong, LU Qi .Chinese experts’ consensus on diagnosis and treatment of tubal pregnancy [J]. Chinese Journal of Practical Gynecology and Obstetrics,2019,35(7):780-787.
[8] PO L, THOMAS J, MILLS K, et al. Guideline no. 414: management of pregnancy of unknown location and tubal and nontubal ectopic pregnancies[J]. Journal of Obstetrics and Gynaecology Canada, 2021, 43(5): 614-630, e1.
[9] 薛伟 , 易福凌 , 王苗 , 等 .孕妇血清亲环素 A和组织型转谷氨酰胺酶水平检测与子痫前期发生不良妊娠的相关性研究 [J]．现代检验医学杂志 , 2021, 36(6): 78-82. XUE Wei, YI Fuling, WANG Miao, et al. Correlation research between serum levels of cyclophilin A as well as tissue transglutaminase in pregnant women and ad-verse pregnancy in preeclampsia [J]. Journal of Modern Laboratory Medicine,2021,36(6): 78-82.
[10] 崔蓉 , 钟兴明．免疫因素与复发性流产 [J].中国医刊 , 2020, 55(3):241-244. CUI Rong, ZHONG Xingming. Immune factors and recurrent spontaneous abortion [J]. Chinese Journal of Medicine,2020,55(3):241-244.
[11] SHENG Yanran, HU Wenting, WEI Chunyan, et al. Insights of efferocytosis in normal and pathological pregnancy[J]. American Journal of Reproductive Immunology (New York, N.Y. 1989), 2019, 82(2): e13088.
[12] 崔佳 , 郭莹 , 高伟 , 等 .妊娠期Ⅱ型固有淋巴细胞相关分子与抗磷脂抗体表达及对妊娠结局的影响 [J].实用医学杂志 , 2019, 35(24):3823-3826. CUI Jia, GUO Ying, GAO Wei, et al. Expression of type Ⅱ intrinsic lymphocyte-associated molecules and anti-phospholipid antibodies during pregnancy and their effects on pregnancy outcomes [J]. The Journal of Practical Medicine,2019,35(24): 3823-3826.
[13] OZLER S, OZTAS E, GULER B G, et al. Increased levels of serum IL-33 is associated with adverse mater-nal outcomes in placenta previa accreta[J].Journal of Maternal-Fetal & Neonatal Medicine, 2021, 34 (19): 3192-3199.
[14] SOHEILYFAR S, NIKYAR T, FATHI M N, et al. Association of IL-10, IL-18, and IL-33 genetic polymorphisms with recurrent pregnancy loss risk in Iranian women[J]. Gynecological Endocrinology, 2019, 35(4): 342-345.
[15] BEGUM S, PERLMAN B E, VALERO-PACHECO N, et al. Dynamic expression of interleukin-33 and ST2 in the mouse reproductive tract is influenced by superovulation[J]. The Journal of Histochemistry and Cytochemistry, 2020, 68(4): 253-267.
[16] SHENG Yanran, HU Wenting, SHEN Huihui, et al. An imbalance of the IL-33/ST2-AXL-efferocytosis axis induces pregnancy loss through metabolic reprogramming of decidual macrophages[J]. Cellular and Molecular Life Sciences, 2022, 79(3): 173.
[17] ARTRU F, BOU SALEH M, MAGGIOTTO F, et al. IL-33/ST2 pathway regulates neutrophil migration and predicts outcome in patients with severe alcoholic hepatitis[J]. Journal of Hepatology, 2020, 72(6): 1052-1061.
[18] MUGERLI S, AMBRO?I? J, GER?AK K, et al. Elevated soluble-ST2 concentrations in preeclampsia correlate with echocardiographic parameters of diastolic dysfunction and return to normal values one year after delivery[J]. The Journal of Maternal-fetal & Neonatal Medicine, 2021, 34(3): 379-385.
[19] ROMERO R, CHAEMSAITHONG P, TARCA A L, et al. Maternal plasma-soluble ST2 concentrations are elevated prior to the development of early and late onset preeclampsia-a longitudinal study[J]. The Journal of Maternal-fetal & Neonatal Medicine, 2018, 31(4): 418-432.

相似文献/References:

[1]闫福堂,苏宝凤,詹颉,等.血清可溶性ST2与血BNP在心力衰竭患者中的相关性研究[J].现代检验医学杂志,2015,30(06):78.[doi:10.3969/j.issn.1671-7414.2015.06.022]
　YAN Fu-tang,SU Bao-feng,ZHAN Jie,et al.Correlation between Serum Soluble ST2 and BNP in Patients with Heart Failure[J].Journal of Modern Laboratory Medicine,2015,30(06):78.[doi:10.3969/j.issn.1671-7414.2015.06.022]
[2]马苑真,董　莉.动脉粥样硬化相关血清生物化学及分子生物学检测指标的最新研究进展[J].现代检验医学杂志,2020,35(03):160.[doi:10.3969 ／ j.issn.1671-7414.2020.03.043]
　MA Yuan-zhen,DONG LI.Recent Advances in the Detection of Serum Biochemistry and Molecular Biology of Atherosclerosis[J].Journal of Modern Laboratory Medicine,2020,35(06):160.[doi:10.3969 ／ j.issn.1671-7414.2020.03.043]
[3]陈志勇a,罗世林a,张　健b,等.慢阻肺患者血清IL-17，IL-27和IL-33水平表达及与不同临床分期FeNO和肺功能的相关性研究[J].现代检验医学杂志,2021,36(04):152.[doi:10.3969/j.issn.1671-7414.2021.04.033]
　CHEN Zhi-yonga,LUO Shi-lina,ZHANG Jianb,et al.Expression of Serum IL-17, IL-27 and IL-33 in Patients with ChronicObstructive Pulmonary Disease and Its Correlation with FeNO and LungFunction in Different Clinical Stages[J].Journal of Modern Laboratory Medicine,2021,36(06):152.[doi:10.3969/j.issn.1671-7414.2021.04.033]
[4]林李娜a,何　微a,刘国栋b,等.变应性鼻炎患儿血清IL-33，ST2水平及IL-33基因rs3939286G/A位点多态性与疾病程度相关性分析[J].现代检验医学杂志,2022,37(03):127.[doi:10.3969/j.issn.1671-7414.2021.03.027]
　LIN Li-naa,HE Weia,LIU Guo-dongb,et al.Correlation Analysis between the Levels of IL-33,ST2 and Polymorphism of IL-33 Gene rs3939286 G/A and Different Degree of Allergic Rhinitis in Children[J].Journal of Modern Laboratory Medicine,2022,37(06):127.[doi:10.3969/j.issn.1671-7414.2021.03.027]

备注/Memo

备注/Memo:
基金项目：遵市科合支撑HZ(2020)161 号。
作者简介：温小英(1967-)，女，本科，主任医师，研究方向：妇科疾病治疗，E-mail：owgfexwh821@21cn.com。
更新日期/Last Update: 2022-11-15