[1]高颖颖,李文冬,温 洁,等.狼疮性肾炎患者血清TRIM27 和FOXO1 表达水平及其与自身免疫抗体和疾病活动的相关性分析[J].现代检验医学杂志,2023,38(01):73-76+82.[doi:10.3969/j.issn.1671-7414.2023.01.014]
　GAO Ying-ying,LI Wen-dong,WEN Jie,et al.Expression of TRIM27 and FOXO1 in Serum of Patients with Lupus Nephritis and Their Correlation with Autoimmune Antibodies and Disease Activity[J].Journal of Modern Laboratory Medicine,2023,38(01):73-76+82.[doi:10.3969/j.issn.1671-7414.2023.01.014]
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狼疮性肾炎患者血清TRIM27 和FOXO1 表达水平及其与自身免疫抗体和疾病活动的相关性分析()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第38卷
期数:
2023年01期
页码:
73-76+82
栏目:
论著
出版日期:
2023-01-15

文章信息/Info

Title:
Expression of TRIM27 and FOXO1 in Serum of Patients with Lupus Nephritis and Their Correlation with Autoimmune Antibodies and Disease Activity
文章编号:
1671-7414（2023）01-073-05
作者:
高颖颖; 李文冬; 温 洁; 张艳琴
（宝鸡市人民医院肾内科，陕西宝鸡 721000）
Author(s):
GAO Ying-ying;  LI Wen-dong;  WEN Jie;  ZHANG Yan-qin
(Department of Nephrology, Baoji People’s Hospital，Shaanxi Baoji 721000, China)
关键词:
狼疮性肾炎; 三结构域蛋白27; 叉头转录因子O1; 疾病活动; 自身免疫抗体
分类号:
R593.242；R392.11
DOI:
10.3969/j.issn.1671-7414.2023.01.014
文献标志码:
A
摘要:
目的 分析狼疮性肾炎（lupus nephritis，LN）患者血清三结构域蛋白27（tripartite motif-containing 27，TRIM27）和叉头转录因子O1（forkhead transcription factor O1，FOXO1）表达水平及其与自身免疫抗体和疾病活动的相关性。方法 选取宝鸡市人民医院2020 年6 月～ 2021 年12 月收治的系统性红斑狼疮（systemic lupus erythematosus，SLE）患者95 例，根据是否并发LN 将其分为LN 组（n=39）和非LN 组（n=56），另选取同期20 例体检健康者作为对照组；测定三组血清TRIM27，FOXO1 水平；对LN 组、非LN 组血清抗ds-DNA 抗体、抗C1q 抗体和抗Sm 抗体进行定性检测。根据肾脏病变活动性指数（activity index，AI）将LN 患者分为LN 活动组（AI 评分≥ 6 分）和LN 非活动组（AI 评分<6 分），比较两组血清TRIM27，FOXO1 水平、血清抗ds-DNA 抗体、抗C1q 抗体和抗Sm 抗体阳性情况及AI 评分；Spearman 法分析LN 患者血清TRIM27，FOXO1 水平与自身免疫抗体阳性率、AI 评分的相关性；ROC曲线分析血清TRIM27 和FOXO1 水平对LN 患者的诊断价值。结果 LN 组血清TRIM27 水平高于非LN 组、对照组（87.63±5.28pg/ml vs 78.82±5.43pg/ml，42.56±5.19pg/ml），血清FOXO1 水平低于非LN 组、对照组（348.36±12.36ng/μl vs 367.15±12.19ng/μl，396.28±12.82ng/μl），差异有统计学意义（F=492.090，100.024，均P<0.05）；LN 组血清抗ds-DNA 抗体、抗C1q 抗体和抗Sm 抗体阳性率高于非LN 组（56.41% vs 35.71%，48.72% vs 28.57%，38.46% vs19.64%），差异均有统计学意义（χ2=3.992，4.010，4.096，均P<0.05）。LN 活动组血清TRIM27 水平、AI 评分高于LN 非活动组（92.51±8.92pg/ml vs 85.19±3.46pg/ml，9.50±2.26 分vs 3.00±1.15 分），血清FOXO1 水平低于LN 非活动组（341.52±12.78ng/μl vs 351.78±12.15ng/μl），血清抗ds-DNA 抗体、抗C1q 抗体和抗Sm 抗体阳性率高于LN非活动组（84.62% vs 42.31%，76.92% vs 34.62%，61.54% vs 26.92%），差异均有统计学意义（t=3.701，11.983，2.444;χ2=0.012，0.013，0.036，均P<0.05）。LN 患者血清TRIM27 水平与血清抗ds-DNA 抗体、抗C1q 抗体和抗Sm 抗体阳性率和AI 评分均呈正相关（r=0.516，0.426，0.458，0.697，均P<0.05），而血清FOXO1 水平与之均呈负相关（r=-0.613，-0.572，-0.482，-0.503，均P<0.05）。ROC 曲线显示，与血清TRIM27 曲线下面积（area under curve，AUC）（AUC=0.822，95%CI: 0.740~0.903），FOXO1（AUC=0.804，95%CI: 0.716~0.893）单项诊断相比，二者联合检测（AUC=0.899，95%CI: 0.840~0.958）对LN 的诊断价值较高（Z=1.698，0.079，均P<0.05）。结论 LN 患者血清TRIM27 高表达、FOXO1 低表达均与自身免疫抗体阳性率和AI 评分相关，对LN 患者有一定诊断价值。
Abstract:
Objective To analyze theexpression of tripartite motif-containing 27 (TRIM27 )and for khead transcription factor O1 (FOXO1) in serum of patients with lupus nephritis(LN) and their correlation with autoimmune antibodies and disease activity. Methods The 95 cases of systemic lupus erythematosus (SLE) patients admitted to Baoji People’s Hospital from June 2020 to December 2021 were selected, and they were divided into LN group (n=39) and non-LN group (n=56) according to whether they had concurrent LN or not. In addition, 20 healthy people who underwent physical examination were gathered as the control group. The levels of serum TRIM27 and FOXO1 in three groups were determined. Anti-ds-DNA antibody, anti-C1q antibody and anti-Sm antibody in serum of LN group and non-LN group were qualitatively detected. According to the nephropathy activity index (AI), LN patients were divided into LN active group (AI score ≥ 6 points) and LN inactive group (AI score < 6 points), the serum TRIM27 and FOXO1 levels, serum anti-ds-DNA antibody, anti-C1q antibody, anti-Sm antibody positive status and AI score were compared between the two groups. Spearman method was used to analyze the correlation of serum TRIM27 and FOXO1 levels with autoimmune antibody positivity and AI score in LN patients. The diagnostic value of serum TRIM27 and FOXO1 levels in patients with LN was analyzed by ROC curve. Results The level of serum TRIM27 in LN group was higher than that in non-LN group and control group (87.63±5.28pg/ml vs 78.82±5.43pg/ml, 42.56±5.19pg/ml), and the level of serum FOXO1 was lower than that in non-LN group and control group (348.36±12.36ng/μl vs 367.15±12.19ng/μl, 396.28±12.82ng/μl), the differences were statistically significant(F=492.090, 100.024, all P<0.05). The positive rates of serum anti-ds-DNA antibody, anti-C1q antibody and anti-Sm antibody in the LN group were higher than those in the non-LN group （56.41% vs 35.71%，48.72% vs 28.57%，38.46% vs 19.64%）, the differences were statistically significant (χ2=3.992, 4.010,4.096, all P<0.05).The level of serum TRIM27 and AI score in the LN active group were higher than those in the LN inactive group(92.51±8.92pg/ml vs 85.19±3.46pg/ml, 9.50±2.26 scores vs 3.00±1.15 scores), the level of serum FOXO1 was lower than that in the LN inactive group(341.52±12.78ng/μl vs 351.78±12.15ng/μl), the positive rates of serum anti-ds-DNA antibody, anti-C1q antibody and anti-Sm antibody in the LN active group were higher than those in the LN inactive group （84.62% vs 42.31%，76.92% vs 34.62%，61.54% vs 26.92%）, the differences were statistically significant (t=3.701,11.983, 2.444, χ2=0.012, 0.013, 0.036, all P<0.05).The level of serum TRIM27 in LN patients was positively correlated with the positive rates of serum anti-ds-DNA antibody, anti-C1q antibody and anti-Sm antibody, and AI score(r=0.516, 0.426,0.458,0.697, all P<0.05), while the level of serum FOXO1 was negatively correlated with them (r=-0.613, -0.572, -0.482, -0.503, all P<0.05).The ROC curve showed that the combined diagnosis area under curve(AUC)（AUC=0.899, 95%CI: 0.840~0.958） was higher than that of TRIM27 (AUC=0.822, 95%CI: 0.740~0.903) and FOXO1 (AUC=0.804, 95%CI: 0.716~0.893) alone (Z=1.698，0.079， P<0.05). Conclusion Serum TRIM27 was highly expressed and FOXO1 was low expressed in LN patients, both of which were related to the positive rate of autoimmune antibodies and AI score, and have certain diagnostic value for LN patients.

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相似文献/References:

[1]陈江秀,莫世松,韩　珠,等.狼疮性肾炎患者尿 IL-17，NGAL 及 HMGB1 水平变化及其临床意义[J].现代检验医学杂志,2021,36(03):68.[doi:10.3969/j.issn.1671-7414.2021.03.015]
　CHEN Jiang-xiu,MO Shi-song,HAN Zhu,et al.Changes and Clinical Significance of Urinary IL-17, NGAL andHMGB1 Levels in Lupus Nephritis[J].Journal of Modern Laboratory Medicine,2021,36(01):68.[doi:10.3969/j.issn.1671-7414.2021.03.015]
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备注/Memo

备注/Memo:
基金项目：陕西省自然科学基础研究计划项目，项目编号：S2019-JC-YB-1405。
作者简介：高颖颖（1992-），女，硕士研究生，副主任医师，研究方向：肾脏病及血液净化，E-mail:gaoyying6668@163.com。
通讯作者：张艳琴（1983-），女，本科，副主任医师，研究方向：慢性肾病、血液透析的临床研究与诊治，E-mail: zhangyanqina@163.com。
更新日期/Last Update: 2023-01-15