[1]毕芳芳,史 敏,程微微,等.黄芪甲苷对缺血性卒中小鼠神经功能保护作用及机制研究[J].现代检验医学杂志,2023,38(01):89-93.[doi:10.3969/j.issn.1671-7414.2023.01.017]
　BI Fang-fang,SHI Min,CHENG Wei-wei,et al.Study on the Protective Effect and Mechanism of Astragaloside IV on Neurological Function in Mice with Ischemic Stroke[J].Journal of Modern Laboratory Medicine,2023,38(01):89-93.[doi:10.3969/j.issn.1671-7414.2023.01.017]
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黄芪甲苷对缺血性卒中小鼠神经功能保护作用及机制研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第38卷
期数:
2023年01期
页码:
89-93
栏目:
论著
出版日期:
2023-01-15

文章信息/Info

Title:
Study on the Protective Effect and Mechanism of Astragaloside IV on Neurological Function in Mice with Ischemic Stroke
文章编号:
1671-7414（2023）01-089-05
作者:
毕芳芳; 史 敏; 程微微; 张 露
（西安培华学院医学检验技术系，西安 710199）
Author(s):
BI Fang-fang;  SHI Min;  CHENG Wei-wei;  ZHANG Lu
（Department of Medical Laboratory Technology, Xi’an Peihua University, Xi’an 710199，China）
关键词:
黄芪甲苷; 缺血性卒中; 炎症; 氧化应激; 焦亡
分类号:
R-332
DOI:
10.3969/j.issn.1671-7414.2023.01.017
文献标志码:
A
摘要:
目的 探讨黄芪甲苷（astragaloside-IV, AST-IV）对缺血性卒中（ischemic stroke）模型鼠神经功能保护作用及机制研究。方法 选取改良线栓法制备大脑中动脉梗死（middle cerebral artery occlusion，MCAO）小鼠模型，小鼠分成四组，每组8 只，分别为假手术组（Sham）、药物对照组（AST-IV）、MCAO 组和治疗组（AST-IV+MCAO）；采用改良神经功能损伤评分（modified neurological severity score，mNSS）实验和转棒（Rotarod）实验综合评价ASTIV的神经保护功能；采用ELISA 法检测AST-IV 对MCAO 脑组织核转录因子(NF-KB)、Toll 样受体4(TLR-4)、肿瘤坏死因子α(TNF-α)、胶质纤维酸性蛋白(glial fibrillary acidic protein ，GFAP)、血红素氧合酶-1(HO-1) 以及半胱天冬酶-1(caspase-1) 表达水平的影响；采用比色法检测AST-IV 对MCAO 脑组织丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽(GSH) 和活性氧（ROS）表达水平的影响。结果 MCAO组的mNSS评分（54.06±8.52）、脑组织ROS（54.06±8.52AFU）、MDA（2.40±0.33 nmol/mg），NF-κB（304.94±39.93pg/mg），TLR4（225.60±37.51pg/mg），TNF-α（80.14±11.06pg/mg），GFAP（181.24±19.88pg/mg）和caspase-1（1.76±0.20）表达均显著高于Sham 组（0.75±0.89，19.85±2.45AFU，1.43±0.29 nmol/mg，131.03±29.45 pg/mg，102.74±19.18 pg/mg，32.04±10.30 pg/mg，63.14±14.48pg/mg，1.00±0.09)，差异具有统计学意义（F=38.572 ～ 184.478，均P<0.001）；MCAO 组在Rotarod 上的停留时间（74.63±18.73s），脑组织SOD（2.88±0.49unin/mg），GSH（3.49±0.64nmol/mg）和HO-1（105.93±24.45pg/ml）表达均显著低于Sham 组（186.50±48.10s，5.58±0.71unit/mg，6.32±0.82 pg/ml，203.11±28.65pg/ml），差异具有统计学意义（F=37.586 ～ 78.889，均P<0.001）；AST-IV+MCAO组的mNSS评分（3.88±1.36）、脑组织ROS（24.64±14.26AFU），MDA（2.00±0.21nmol/mg），NF-κB（212.03±38.63 pg/mg），TLR-4（134.81±25.60 pg/mg），TNF-α（53.61±12.49pg/mg），GFAP（101.00±15.04 pg/mg）和caspase-1（1.46±0.15）表达均显著低于MCAO 组，差异具有统计学意义（F=4.639 ～ 82.890， 均P<0.05）；AST-IV+MCAO 组在Rotarod 上的停留时间（160.75±33.33s）， 脑组织SOD（4.51±0.51unit/mg），GSH（5.30±0.73nmol/mg）和HO-1（179.96±20.97pg/ml）均显著高于MCAO 组，差异具有统计学意义（F=27.681 ～ 42.364，均P<0.001）。结论 AST-IV 的脑保护作用可能与其抗炎、抗氧化应激、抑制焦亡进程有关。
Abstract:
Objective To investigate the protective effect and mechanism of astragaloside IV（AST-IV）in a mouse model of ischemic stroke. Methods The middle cerebral artery occlusion(MCAO) mouse model was established by the modified suture method. The mice were divided into four groups with 8 mice in each group, namely the Sham group, the AST-IV group, the MCAO group and the AST-IV+MCAO group. The neuroprotective function of AST-IV was comprehensively evaluated by modified neurological severity score (mNSS) test and Rotarod test. ELISA was used to detect the effect of AST-IV on the expression levels of nuclear factor kappa-B （NF-κB）, Toll like receptor 4（TLR-4）, Tumor necrosis factor-α（TNF-α）, glial fibrillary acidic protein (GFAP), heme oxygenase-1 (HO-1) and caspase-1in MCAO brain tissue. Colorimetry was used to detect the effect of AST-IV on the expression levels of malondialdehyde (MDA), Superoxide dismutase（SOD）, glutathione (GSH) and reactive oxygen species（ROS） in MCAO brain tissue. Results The mNSS score（54.06±8.52） in MCAO group, the expression of ROS（54.06±8.52 AFU）, MDA（2.40±0.33 nmol/mg）, NF-κB（304.94±39.93pg/mg）,TLR-4（225.60±37.51pg/mg）, TNF-α（80.14±11.06pg/mg）, GFAP （181.24±19.88pg/mg）and caspase-1（1.76±0.20） in brain tissue were significantly higher than those in the Sham group（0.75±0.89，19.85±2.45AFU，1.43±0.29 nmol/mg， 131.03±29.45 pg/mg，102.74±19.18 pg/mg，32.04±10.30 pg/mg，63.14±14.48 pg/mg，1.00±0.09), and the differences were statistically significant (F=8.572 ～ 184.478, all P<0.001). The MCAO group had a longer residence time （74.63±18.73s） on Rotarod, the expression of SOD（2.88±0.49unin/mg）, GSH（3.49±0.64nmol/mg） and HO-1（105.93±24.45pg/ml）in brain tissue were significantly lower than those in the Sham group（186.50±48.10s，5.58±0.71unit/mg，6.32±0.82 pg/ml， 203.11±28.65pg/ml）, and the differences were statistically significant (F =37.586~78.889, all P<0.001). The mNSS score （3.88±1.36） in the AST-IV+MCAO group, the expression of ROS（24.64±14.26AFU）, MDA（2.00±0.21nmol/mg）, NF-κB（212.03±38.63 pg/mg）, TLR-4（134.81±25.60 pg/mg）, TNF -α（53.61±12.49 pg/mg）, GFAP（101.00±15.04 pg/mg） and caspase-1 （1.46±0.15）in the brain tissue were significantly lower than MCAO group, and the differences were statistically significant (F=4.639 ～ 82.890，all P<0.05). AST-IV+MCAO group stay on Rotarod time（160.75±33.33s）, the expression of SOD（4.51±0.51unit/mg）, GSH（5.30±0.73nmol/mg）and HO-1（179.96±20.97pg/ml）were significantly higher than those in the MCAO group, and the differences were statistically significant (F=27.681 ～ 42.364, all P<0.001). Conclusion The brain protective effect of AST-IV may be related to its anti inflammation, anti-oxidative stress, and inhibition of coke death process.

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备注/Memo

备注/Memo:
基金项目：陕西省教育厅科研计划项目（21JK0820），陕西省自然科学基础研究计划（2022JQ-876，2022JM-558）。
作者简介：毕芳芳（1991-），女，硕士研究生，讲师，研究方向：神经炎症，E-mail: 2014zora@sina.com。
通讯作者：张露（1987-），男，硕士研究生，主管技师，E-mail:459688381@qq.com。
更新日期/Last Update: 2023-01-15