[1]牛立远a,刘会雪a,史立彬b.急性脑梗死患者血清CCR5 和miR-211-5p 表达水平及其临床意义[J].现代检验医学杂志,2023,38(01):128-134.[doi:10.3969/j.issn.1671-7414.2023.01.024]
　NIU Li-yuana,LIU Hui-xuea,SHI Li-binb.Expression Levels and Their Clinical Significance of Serum CCR5 and miR-211-5p in Patients with Acute Cerebral Infarction[J].Journal of Modern Laboratory Medicine,2023,38(01):128-134.[doi:10.3969/j.issn.1671-7414.2023.01.024]
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急性脑梗死患者血清CCR5 和miR-211-5p 表达水平及其临床意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第38卷
期数:
2023年01期
页码:
128-134
栏目:
论著
出版日期:
2023-01-15

文章信息/Info

Title:
Expression Levels and Their Clinical Significance of Serum CCR5 and miR-211-5p in Patients with Acute Cerebral Infarction
文章编号:
1671-7414（2023）01-128-07
作者:
牛立远a; 刘会雪a; 史立彬b
（石家庄平安医院 a. 急诊内科；b. 急诊科，石家庄 050021）
Author(s):
NIU Li-yuana;  LIU Hui-xuea;  SHI Li-binb
(a.Emergency Department of Internal Medicine; b.Department of Emergency, Shijiazhuang Ping’an Hospital, Shijiazhuang 050021,China)
关键词:
急性脑梗死; CC 趋化因子细胞受体5; 微小核糖核酸-211-5p
分类号:
R743.3；R392.11
DOI:
10.3969/j.issn.1671-7414.2023.01.024
文献标志码:
A
摘要:
目的 探究急性脑梗死（acute cerebral infarction）患者血清CC 趋化因子细胞受体5（chemokine C-C motifreceptor type 5，CCR5）和微小核糖核酸（microRNA，miR）-211-5p 表达水平及其临床意义。方法 选取2019 年2 月~2021 年2 月石家庄平安医院收治的130 例急性脑梗死患者（观察组），另选同期130 例健康体检者作为对照（对照组）。实时荧光定量PCR 检测血清中CCR5 信使RNA（messengerRNA，mRNA）和miR-211-5p 相对表达水平；酶联免疫吸附试验（enzyme-linked immunosorbent assay，ELISA）法检测血清中CCR5 水平；Pearson 法分析CCR5 mRNA和miR-211-5p 表达水平与美国国立卫生研究院卒中量表（the National Institutes of Health Stroke Scale，NIHSS）评分相关性；分析CCR5 mRNA 和miR-211-5p 表达水平与急性脑梗死患者临床病理特征关系；受试者工作特征（receiveroperating characteristic，ROC）曲线分析CCR5 mRNA 和miR-211-5p 表达水平对急性脑梗死的诊断价值；Kaplan-Meier生存曲线分析CCR5 mRNA 和miR-211-5p 表达水平与急性脑梗死预后生存的关系。结果 与对照组相比，观察组患者血清中CCR5 mRNA 表达（1.49±0.29 vs 1.03±0.24）和蛋白含量水平（290.96±10.35ng/ml vs 152.37±8.97ng/ml）显著增高，miR-211-5p 相对表达水平（0.59±0.18 vs 0.96±0.22）显著降低，差异均有统计学意义（t=13.933，115.374，14.841，均P ＜ 0.001） ；轻度组、中度组、重度组CCR5 mRNA（1.26±0.21，1.58±0.22，1.77±0.24）和蛋白含量水平（257.34±7.26ng/ml，289.31±9.36ng/ml，357.31±8.12ng/ml）依次增加，miR-211-5p 相对表达水平（0.74±0.17，0.61±0.1，0.28±0.18）依次降低，差异均有统计学意义（F=57.145，1 396.953，73.759，均P ＜ 0.001）。急性脑梗死患者CCR5 mRNA 表达水平与miR-211-5p 表达水平呈负相关性（r=-0.341，P<0.001），与NIHSS 评分呈正相关性（r=0.315，P<0.001）；miR-211-5p 表达水平与NIHSS 评分具有显著的负相关性（r=-0.475，P<0.001）。CCR5 mRNA和miR-211-5p 表达水平与急性脑梗死患者脑梗死体积、OCSP 分型及血脂异常有关（P ＜ 0.05），与性别、梗死部位无关（P>0.05）。CCR5 mRNA 和miR-211-5p 诊断急性脑梗死的曲线下面积为0.834，0.868，二者联合诊断的曲线下面积为0.921。CCR5 mRNA 高表达患者的90 天生存率显著低于低表达患者（61.97% vs 81.36%），miR-211-5p 高表达患者的90 天生存率显著高于低表达患者（80.65% vs 61.76%），差异均有统计学意义（χ2=5.853，5.589，均P ＜ 0.05）。结论 急性脑梗死患者血清CCR5 mRNA 和蛋白表达水平增高，miR-211-5p 表达水平降低，均与患者的病情程度有关，对急性脑梗死有一定诊断价值。
Abstract:
Objective To explore the expression levels and clinical significance of serum chemokine C-C motif receptor type 5 (CCR5) and microRNA-211-5p (miR-211-5p) in patients with acute cerebral infarction. Methods A total of 130 patients with acute cerebral infarction admitted to Shijiazhuang Ping’an Hospital from February 2019 to February 2021 were selected (observation group), and 130 healthy subjects during the same period were selected as control group (control group). Real-time fluorescent quantitative PCR was used to detect the relative expression levels of CCR5 messenger RNA(mRNA) and miR-211-5p in serum.Enzyme-linked immunosorbent assay(ELISA) method to detect the level of CCR5 in serum. Pearson was used to analyze the correlation between the expression levels of CCR5 mRNA and miR-211-5p and the National Institutes of Health Stroke Scale (NIHSS) score. Analysis of the relationship between the expression levels of CCR5 mRNA and miR-211-5p and the clinicopathological characteristics of patients with acute cerebral infarction. Receiver operating characteristic curve (ROC curve) was used to analyze the diagnostic value of CCR5 mRNA and miR-211-5p expression levels for acute cerebral infarction. Kaplan-Meier survival curve analysis of the relationship between the expression levels of CCR5 mRNA and miR-211-5p and the prognosis and survival of acute cerebral infarction. Results Compared with the control group, the relative expression level of CCR5 mRNA expression(1.49±0.29 vs 1.03±0.24) and protein content level (290.96±10.35ng/ml vs 152.37±8.97ng/ml) in the serum of patients in the observation group was significantly increased, and the relative expression level of miR-211-5p (0.59±0.18 vs 0.96±0.22) was significantly reduced (t=13.933,115.374, 14.841,all P<0.05). CCR5 mRNA (1.26±0.21 , 1.58±0.22 , 1.77±0.24) and protein levels (257.34±7.26ng/ml , 289.31±9.36ng/ml, 357.31±8.12ng/ml) in mild, moderate and severe groups increased sequentially, and the relative expression level of miR-211-5p (0.74±0.17 , 0.61±0.15 , 0.28±0.18) decreased sequentially, and the difference was statistically significant (F=54.861, 1 296.567, 68.629, all P<0.001). The expression level of CCR5 mRNA in patients with acute cerebral infarction had a significant negative correlation with the expression level of miR-211-5p (r=-0.341, P<0.001), and a significant positive correlation with the NIHSS score (r=0.315, P<0.001). The expression level of miR-211-5p had a significant negative correlation with NIHSS score (r=-0.475, P<0.001). The expression levels of CCR5 mRNA and miR-211-5p were related to cerebral infarction volume, OCSP classification and dyslipidemia in patients with acute cerebral infarction (P<0.05), but not related to gender and infarct location (P>0.05). The areas under the curve of CCR5 mRNA and miR-211-5p in the diagnosis of acute cerebral infarction were 0.834 and 0.868, and the area under the curve of the combined diagnosis of the two was 0.921.The 90-day survival rate of patients with high expression of CCR5 mRNA was significantly lower than that of patients with low expression(61.97% vs 81.36%)，and the 90-day survival rate of patients with high expression of miR-211-5p was significantly higher than that of patients with low expression (80.65% vs 61.76%),the differences were statistical significant(χ2=5.853, 5.589, P<0.05) Conclusion In the expression levels of serum CCR5 mRNA and protein in patients with acute cerebral infarction were increased, and the expression levels of miR-211-5p were decreased, which were related to the severity of the disease, and have certain diagnostic value for acute cerebral infarction.

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备注/Memo

备注/Memo:
基金项目：河北省中医药管理局科研项目（2010132）。
作者简介：牛立远（1980-），男，本科，主治医师，研究方向：内科，E-mail：79156591@qq.com。
更新日期/Last Update: 2023-01-15