[1]康会贤a,张 蕾a,马夕锐a,等.孕妇早期血清miR-29a-3p 和VEGFA 表达水平及其对子痫前期预测价值研究[J].现代检验医学杂志,2023,38(02):32-36.[doi:10.3969/j.issn.1671-7414.2023.02.006 ]
　KANG Hui-xiana,ZHANG Leia,MA Xi-ruia,et al.Study on the Expression Level of Serum miR-29a-3p and VEGFA in Early Pregnancy and Its Predictive Value for Preeclampsia[J].Journal of Modern Laboratory Medicine,2023,38(02):32-36.[doi:10.3969/j.issn.1671-7414.2023.02.006 ]
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孕妇早期血清miR-29a-3p 和VEGFA 表达水平及其对子痫前期预测价值研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第38卷
期数:
2023年02期
页码:
32-36
栏目:
论著
出版日期:
2023-03-15

文章信息/Info

Title:
Study on the Expression Level of Serum miR-29a-3p and VEGFA in Early Pregnancy and Its Predictive Value for Preeclampsia
文章编号:
1671-7414（2023）02-032-05
作者:
康会贤a; 张 蕾a; 马夕锐a; 崔照领b
（石家庄市妇幼保健院 a.产七科；b.妇产科，石家庄 050000）
Author(s):
KANG Hui-xiana;  ZHANG Leia;  MA Xi-ruia;  CUI Zhao-lingb
(a. the Seventh Department of Obstetrics; b. Department of Obstetrics and Gynecology, Shijiazhuang Maternal and Child Health Hospital, Shijiazhuang 050000, China)
关键词:
子痫前期; 微小核糖核酸 -29a-3p; 血管内皮生长因子 A
分类号:
R714.244；R392.11
DOI:
10.3969/j.issn.1671-7414.2023.02.006
文献标志码:
A
摘要:
目的 研究微小核糖核酸（micro RNA，miR）-29a-3p和血管内皮生长因子 A（vascular endothelial growth factor A ,VEGFA）在子痫前期患者血清中的表达及意义。方法 前瞻性选取 2018年 1月～2021年 1月在石家庄市妇幼保健院行产检的 600例孕妇为研究对象，对孕妇随访至分娩结束，根据孕妇是否患子痫前期，分为子痫前期组（n=60）和非子痫前期组（n=540）。实时荧光定量 PCR法检测在孕 10～14周入院产检时血清中 miR-29a-3p的水平，采用酶联免疫吸附法（ELISA）测定血清 VEGFA水平；采用 Pearson法分析 miR-29a-3p水平与 VEGFA的相关性。采用受试者工作特征（ROC）曲线评价血清 miR-29a-3p和 VEGFA以及二者联合检测预测子痫前期的价值。结果 子痫前期组 miR-29a-3p（2.05±0.54）水平高于非子痫前期组（1.04±0.29），VEGFA水平（34.38±9.25 ng/L)低于非子痫前期组（69.49±19.57 ng/L），差异具有统计学意义（t=22.951,13.824，均 P＜ 0.05）。重度组血清中 VEGFA水平（24.05±6.41 ng/L）显著低于轻度组（38.81±10.47 ng/L），miR-29a-3p（2.45±0.61）水平显著高于轻度组（1.88±0.51），差异均有统计学意义（t=5.537，3.738，均 P＜ 0.05）。 Pearson分析显示，子痫前期患者血清中 miR-29a-3p与 VEGFA呈负相关（r= -0.601，P＜ 0.05）。 ROC曲线显示，血清中 miR-29a-3p和 VEGFA水平预测子痫前期的曲线下面积（AUC）分别为 0.904，0.882；两者联合预测子痫前期的 AUC为 0.937，其灵敏度和特异度分别为 80.00%，95.20%。结论 子痫前期患者血清中 miR-29a-3p表达上升，而 VEGFA表达下降，血清 miR-29a-3p和 VEGFA对子痫前期具有一定的预测价值。
Abstract:
Objective To study the expression and significance of microRNA (miR) -29a-3p and vascular endothelial growth factor A (VEGFA) in the serum of patients with preeclampsia. Methods Prospectively selected of 600 pregnant women undergoing maternity check-ups in Shijiazhuang Maternal and Child Health Hospital from January 2018 to January 2021 were selected as the research objects. The pregnant women were followed up to the end of delivery, according to whether the pregnant women had preeclampsia, they were divided into preeclampsia group (n=60) and non-preeclampsia group (n=540). The level of miR-29a-3p in serum was detected by real-time fluorescence quantitative PCR method at the 10th to 14th week of gestation at the time of admission for obstetric examination. Enzyme-linked immunosorbent assay (ELISA) was used to determine the level of serum VEGFA, Pearson method was used to analyze the correlation between miR-29a-3p level and VEGFA, and receiver operating characteristic (ROC) curve was used to evaluate the value of serum miR-29a-3p, VEGFA, and the combined detection in predicting preeclampsia. Results MiR-29a-3p level (2.05±0.54 ) in the preeclampsia group were higher than those in the non-preeclampsia group(1.04±0.29), the level of VEGFA (34.38±9.25 ng/L) was lower than that of the non-preeclampsia group(69.49±19.57 ng/L), and the differences were statistically significant (t=22.951,13.724,all P<0.05). The level of serum VEGFA in the severe group (24.05±6.41 ng/L) was significantly lower than that in the mild group (38.81±10.47 ng/L), and the level of miR-29a-3p (2.45±0.61) was significantly higher than that of the mild group (1.88±0.51)，and the differences were statistically significant (t=5.537, 3.738, all P<0.05). Pearson analysis showed that the level of serum miR-29a-3p in patients with preeclampsia was negatively correlated with VEGFA (r=-0.601, P<0.05). The ROC curve showed that the area under the curve (AUC) of serum miR-29a-3p and VEGFA levels predicting preeclampsia was 0.904 and 0.882, respectively, The AUC of the two combined prediction of preeclampsia was 0.937, and the sensitivity and specificity were 80.00% and 95.20%, respectively. Conclusion The expression of miR-29a-3p in the serum of patients with preeclampsia increases, while the expression of VEGFA decreases. The serum miR-29a-3p and VEGFA had certain predictive value for preeclampsia.

参考文献/References:

[1] 任丹玉 , 王永红 . 凝血功能指标和血小板参数对子痫前期及其严重程度的辅助诊断价值研究 [J].中国全科医学 , 2019, 22(22):2698-2704. REN Danyu, WANG Yonghong. Assistant diagnosis for coagulation function and platelet parameters in the diagnosis of preeclampsia and its severity [J]. Chinese General Practice, 2019, 22(22):2698-2704.
[2] 方章华 , 秦田瑞 , 邓晨晨 , 等 . 凝血、血小板及生化指标对重度子痫前期的辅助诊断价值研究 [J]. 实用妇产科杂志 , 2020, 36(12): 936-940. FANG Zhanghua, QIN Tianrui, DENG Chenchen, et al. The value of coagulation, platelet and biochemical indicators in assistant diagnosis of severe preeclampsia [J]. Journal of Practical Obstetrics and Gynecology, 2020, 36(12):936-940.
[3] POON L C, SHENNAN A, HYETT J A, et al, The in-ternational federation of gynecology and obstetrics(fi-GO) initiative on pre-eclampsia:a pragmatic guide for first-trimester screening and prevention[J]. International Journal of Gynecology & Obstetrics, 2019, 145(Suppl 1): 1-33.
[4] KHALIQ O P, MURUGESAN S, MOODLEY J, et al. Differential expression of miRNAs are associated with the insulin signaling pathway in preeclampsia and gestational hypertension[J]. Clinical and Experimental Hypertension, 2018, 40(8): 744-751.
[5] 孙嘉敏 , 陈先侠 . miRNA-29b及血管内皮生长因子在子痫前期病程中的作用机制 [J]. 解放军医学院学报 , 2019, 40(11): 1074-1078. SUN Jiamin, CHEN Xianxia. Changes and relationship of miRNA-29b and VEGF in preeclampsia[J]. Academic Journal of Chinese PLA Medical School, 2019, 40(11):1074-1078.
[6] 谢幸 , 苟文丽 . 妇产科学 [M]. 8版 . 北京：人民卫生出版社 , 2013：64-71. XIE Xing, GOU Wenli. Obstetrics and Gynecology[M]. 8th Ed, Beijing: People’s Health Publishing House, 2013:64-71.
[7] 中华医学会妇产科学分会妊娠期高血压疾病学组 . 妊娠期高血压疾病诊治指南 (2015)[J]. 中华围产医学杂志 , 2016, 19(3):161-169. Hypertensive Disorders in Pregnancy Subgroup, Chinese Society of Obstetrics and Gynecology, Chinese Medical Association. Diagnosis and treatment guideline of hypertensive disorders in pregnancy (2015)[J]. Chinese Journal of Obstetrics and Gynecology, 2016, 19(3):161-168.
[8] 危丽华 , 张新 , 封伟 . 子痫前期孕妇血清 SOCS3, PP-13, GATA-3水平变化及其预测价值研究 [J]. 现代检验医学杂志 , 2022, 37(1):48-51, 87. WEI Lihua, ZHANG Xin, FENG Wei. Changes of serum SOCS3, PP-13 and GATA-3 levels in preeclampsia and their predictive value [J]. Journal of Modern Laboratory Medicine, 2022, 37(1):48-51, 87.
[9] 陈维 , 王子莲 . 双胎妊娠并发子痫前期的发病趋势、特征及危险因素分析 [J]. 中山大学学报 (医学科学版 ), 2021, 42(1): 145-153. CHEN Wei, WANG Zilian. Analysis of the tendency, characteristic and risk factors of twin pregnancy complicated with preeclampsia [J]. Journal of Sun Yat-sen University(Medical Sciences), 2021, 42(1):145-153.
[10] 曹芬 , 孙素芳 , 胡文胜 . 子痫前期患者外周血微小 RNA-210水平与胎盘组织血管内皮生长因子、环氧化酶 -2及患者预后的关系 [J]. 中国卫生检验杂志 , 2020, 30(17): 2144-2147. CAO Fen, SUN Sufang, HU Wensheng. Relationship between miRNA-210 level in peripheral blood of preeclampsia patients and placental vascular growth factor, COX-2 and prognosis [J]. Chinese Journal of Health Laboratory Technology , 2020, 30(17):2144-2147.
[11] 王芳 . 血清维生素水平、氧化应激损伤与孕妇子痫前期的相关性研究 [J]. 实用临床医药杂志 , 2020, 24(15): 87-89. WANG Fang, Correlation between serum vitamin level, oxidative stress injury and preeclampsia in pregnant women[J]. Journal of Clinical Medicine in Practice, 2020, 24(15):87-89.
[12] 薛伟 , 易福凌 , 王苗 , 等 . 孕妇血清亲环素 A和组织型转谷氨酰胺酶水平检测与子痫前期发生不良妊娠的相关性研究 [J]. 现代检验医学杂志 , 2021, 36(6): 78-82. XUE Wei, YI Fuling, WANG Miao, et al. Correlation research between serum levels of Cyclophilin A as well as tissue transglutaminase in pregnant women and adverse pregnancy in preeclampsia [J]. Journal of Modern Laboratory Medicine, 2021, 36(6):78-82.
[13] 张琼芳 , 杨宁 , 王彬苏 , 等 . Elabela在子痫前期胎盘中的表达及其意义 [J]. 中华高血压杂志 , 2019, 27(4): 349-355. ZHANG Qiongfang, YANG Ning, WANG Binsu, et al. The expression of Elabela in preeclamptic placenta [J]. Chinese Journal of Hypertension, 2019, 27(4):349-355.
[14] WITVROUWEN I, MANNAERTS D, RATAJCZAK J, et al. MicroRNAs targeting VEGF are related to vascular dysfunction in preeclampsia[J]. Bioscience Reports, 2021, 41(8):BSR20210874.
[15] AGARWAL R, KUMARI N, KAR R, et al, Evaluation of placental VEGFA mRNA expression in preeclampsia: a case control study[J]. The Journal of Obstetrics and Gynecology of India, 2019, 69(2): 142-148.
[16] JEON H H, YU Quan, LU Yongjian, et al. FOXO1 reg-ulates VEGFA expression and promotes angiogenesis in healing wounds[J]. Journal of Pathology, 2018, 245(3): 258-264.
[17] ZHOU Situo, LIANG Pengfei, ZHANG Pihong, et al. The long noncoding RNA PDK1-AS/miR-125b-5p/ VEGFA axis modulates human dermal microvascular endothelial cell and human umbilical vein endothelial cell angiogenesis after thermal injury[J]. Journal of Cellular Physiology, 2021, 236(4): 3129-3142.
[18] MEI Zhixiong, HUANG Baoqin, ZHANG Yuan, et al. Histone deacetylase 6 negatively regulated microRNA-199a-5p induces the occurrence of preeclampsia by targeting VEGFA in vitro[J]. Biomedicine & Pharmacotherapy, 2019, 114(1): 108805.
[19] KOLKOVA Z, HOLUBEKOVA V, GRENDAR M, et al. Association of circulating miRNA expression with preeclampsia, its onset, and severity[J]. Diagnostics (Basel, Switzerland), 2021, 11(3): 476.
[20] BOUNDS K R, CHIASSON V L, PAN L J, et al. MicroRNAs: New players in the pathobiology of preeclampsia[J]. Frontiers in Cardiovascular Medicine, 2017, 4(1): 60.
[21] LU Guodong, CHENG Peng, LIU Ting, et al. BMSC-derived exosomal miR-29a promotes angiogenesis and osteogenesis[J]. Front Cell Dev Biol, 2020, 8(1): 608521.
[22] MAUCHER D, SCHMIDT B, SCHUMANN J. Loss of endothelial barrier function in the inflammatory setting:indication for a cytokine-mediated post-transcriptional mechanism by virtue of upregulation of miRNAs miR-29a-3p, miR-29b-3p, and miR-155-5p[J]. Cells (Basel, Switzerland), 2021, 10(11): 2843.
[23] 周美芳 .子痫前期患者肾脏血流动力学参数与血管内皮损伤因子的相关性研究 [J]. 现代实用医学 , 2019, 31(7): 866-868. ZHOU Meifang. Correlation between renal hemodynamic parameters and vascular endothelial injury factor in patients with preeclampsia [J]. Modern Practical Medicine, 2019, 31(7):866-868.

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备注/Memo

备注/Memo:
收稿日期：2022-07-07修回日期：2022-10-31
基金项目：河北省医学科学研究重点课题（NO.20200145）。
作者简介：康会贤（1984-），女，本科，主治医师，研究方向：妇产科， E-mail：khxkang@163.com。
通讯作者：崔照领（1978-），女，硕士，主任医师，研究方向：妇产科， E-mail：uiiueh@163.com。

更新日期/Last Update: 2023-03-15