[1]殷运林,陈东颖,杨 康.卵巢子宫内膜异位症患者血清磷脂酰胆碱、苯丙氨酰- 异亮氨酸表达水平及临床价值研究[J].现代检验医学杂志,2023,38(02):43-47+185.[doi:10.3969/j.issn.1671-7414.2023.02.008 ]
　YIN Yun-lin,CHEN Dong-ying,YANG Kang.Study on the Expression of Phosphatidylcholine and Phenylalanyl Isoleucine in Serum of Patients with Ovarian Endometriosis and Its Clinical Value[J].Journal of Modern Laboratory Medicine,2023,38(02):43-47+185.[doi:10.3969/j.issn.1671-7414.2023.02.008 ]
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卵巢子宫内膜异位症患者血清磷脂酰胆碱、苯丙氨酰- 异亮氨酸表达水平及临床价值研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第38卷
期数:
2023年02期
页码:
43-47+185
栏目:
论著
出版日期:
2023-03-15

文章信息/Info

Title:
Study on the Expression of Phosphatidylcholine and Phenylalanyl Isoleucine in Serum of Patients with Ovarian Endometriosis and Its Clinical Value
文章编号:
1671-7414（2023）02-043-06
作者:
殷运林1; 陈东颖1; 杨 康2
（1.亳州市人民医院妇产科，安徽亳州 236000；2. 蚌埠医学院第一附属医院妇产科，安徽蚌埠 233099）
Author(s):
YIN Yun-lin1;  CHEN Dong-ying1;  YANG Kang2
（1. Department of Obstetrics and Gynecology，Bozhou People’s Hospital, Anhui Bozhou 236000, China; 2. Department of Obstetrics and Gynecology, the first Affiliated Hospital of Bengbu Medical College , Anhui Bengbu 233099，China）
关键词:
卵巢子宫内膜异位症; 磷脂酰胆碱; 苯丙氨酰 -异亮氨酸
分类号:
R711.71; R446.112
DOI:
10.3969/j.issn.1671-7414.2023.02.008
文献标志码:
A
摘要:
目的 探讨卵巢子宫内膜异位症（ovary endometriosis，OEM）患者血清磷脂酰胆碱（phosphatidylcholine， PC）、苯丙氨酰 -异亮氨酸（phenylalanyl-isoleucine, PheIL）表达水平及临床价值。方法 选取 2020年 1月～2022年 1月期间亳州市人民医院诊治的 OEM患者 163例为研究对象（OEM组），根据患者术后复发情况分为复发组（n=35）和未复发组（n=128）。以同期因卵巢良性疾病诊治的 60例女性患者为对照组。应用高效液相色谱 -串联质谱法（high performance liquid chromatography tandem mass spectrometry，HPLC-MS/MS）检测各组血清 PC和 PheIL水平。比较不同美国生殖医学会（American Society of Reproductive Medicine，ASRM）分期、痛经程度 OEM患者血清 PC和 PheIL水平差异。单因素及多因素 COX回归分析影响 OEM复发的危险因素。受试者工作曲线评价血清 PC，PheIL及联合检测对 OEM复发的诊断价值。结果 OEM组患者血清 PC（34∶3）（18.01±3.66μg/ml），PheIL（64.38±7.75 ng/ml）均高于对照组（1.66±0.47μg/ml，42.66±6.50ng/ml），差异具有统计学意义 (t=34.388，18.145，均 P <0.05)。ASRM分期Ⅲ～Ⅳ期的 OEM患者血清 PC（34∶3）（21.74±3.71μg/ml），PheIL水平（71.22±7.89ng/ml）高于Ⅰ～Ⅱ期患者（14.41±3.50μg/ml，57.79±7.60ng/ml），差异具有统计学意义 (t=12.979，11.069，均 P<0.05)。ASRM分期Ⅲ～Ⅳ期（OR=1.510, P<0.001），PC（34∶3）升高（OR=1.446, P<0.001），PheIL升高（OR=1.706，P<0.001）是影响 OEM患者术后复发的独立危险因素，术后妊娠（OR=0.736，P<0.001）和术后用药（OR=0.749，P<0.001）是影响 OEM患者术后复发的保护因素。血清 PC（34∶3）和 PheIL联合检测对 OEM复发诊断的曲线下面积（95%CI）为 0.864(0.840～0.879)，大于血清 PC（34∶3）和 PheIL单一指标检测 0.772(0.735～0.808)，0.727(0.683～0.770)，差异具有统计学意义 (Z=4.252，5.640，均 P <0.001)。结论 OEM患者血清 PC和 PheIL水平升高 ,两者与 ASRM分期有关，两者联合检测有助于预测 OEM复发。
Abstract:
Objective To investigate the serum levels of phosphatidylcholine (PC) and phenylalanyl-isoleucine (PheIL) in patients with ovary endometriosis (OEM) and their clinical significance. Methods 163 OEM patients diagnosed and treated in Bozhou People’s Hospital from January 2020 to January 2022 were selected as the study object (OEM group). According to the recurrence of patients after surgery, they were divided into recurrence group (n=35) and non-recurrence group (n=128). 60 women with benign ovarian diseases during the same period were selected as control group. Serum PC and PheIL levels in each group were detected by high performance liquid chromatography tandem mass spectrometry（HPLC-MS/MS）. The levels of serum PC and PheIL in OEM patients with different American Society of Reproductive Medicine（ASRM）stages and dysmenorrhea were compared. Factors affecting OEM recurrence were analyzed by univariate and multivariate COX regression analysis. Receiver operating curve was used to evaluate the diagnostic value of serum PC, PheIL and combined detection for OEM recurrence. Results Serum PC (34∶3) (18.01 ± 3.66μg/ml), PheIL (64.38±7.75 ng/ml ) of patients in OEM group （1.66±0.47μg /ml，42.66 ± 6.50 ng/ml）were higher than those in the control group, and the differences were statistically significant (t=34.388, 18.145, all P<0.05). Serum PC (34∶3) (21.74 ± 3.71μg /ml ), and the PheIL level (71.22 ± 7.89ng/ml ) of OEM patients in ASRM stage Ⅲ～Ⅳ was higher than that in patients with stage I to II（14.41±3.50μg /ml，57.79 ± 7.60ng/ ml）, the differences were statistically significant (t=12.979, 11.069, all P <0.05). ASRM stage Ⅲ ~ Ⅳ (OR=1.510, P<0.001), increased PC (34∶3) (OR=1.446, P<0.001), and increased PheIL (OR=1.706, P<0.001) were independent risk factors for postoperative recurrence of OEM patients. Postoperative pregnancy (OR=0.736, P<0.001) and postoperative medication (OR=0.749, P<0.001) were protective factors for postoperative recurrence of OEM patients. The area under the curve (95% CI) for OEM recurrence diagnosis by combined detection of serum PC (34∶3) and PheIL was 0.864 (0.840～0.879), which was higher than that by single detection of serum PC (34∶3) and PheIL (0.772 (0.735～0.808), 0.727 (0.683～0.770), with statistically significant difference (Z=4.252, 5.640, P=0.000, 0.000). Conclusion Serum PC and PheIL levels in patients with OEM were elevated, and they were related to ASRM stage. The combined detection of the two can help predict the recurrence of OEM.

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备注/Memo

备注/Memo:
收稿日期：2022-10-28修回日期：2022-12-15
基金项目：安徽省卫生计生委科研计划项目（2019QK005）。
作者简介：殷运林（1990-），男，硕士研究生，主治医师，研究方向：妇产科， E-mail：yunlinjia3@163.com。

更新日期/Last Update: 2023-03-15