[1]吴莉芳,张 麟,张 杰,等.变应性鼻炎患者血清PTX3，LXA4 表达水平及临床价值研究[J].现代检验医学杂志,2023,38(02):140-145.[doi:10.3969/j.issn.1671-7414.2023.02.026 ]
　WU Li-fang,ZHANG Lin,ZHANG Jie,et al.Serum PTX3 and LXA4 Levels in Patients with Allergic Rhinitis and Their Clinical Significance[J].Journal of Modern Laboratory Medicine,2023,38(02):140-145.[doi:10.3969/j.issn.1671-7414.2023.02.026 ]
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变应性鼻炎患者血清PTX3，LXA4 表达水平及临床价值研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第38卷
期数:
2023年02期
页码:
140-145
栏目:
论著
出版日期:
2023-03-15

文章信息/Info

Title:
Serum PTX3 and LXA4 Levels in Patients with Allergic Rhinitis and Their Clinical Significance
文章编号:
1671-7414（2023）02-140-06
作者:
吴莉芳1; 张 麟1; 张 杰2; 王占军1
（1.大同市第五人民医院耳鼻咽喉头颈外科，山西大同 037000；2.大同大学医学院耳鼻咽喉科教研室，山西大同 037000）
Author(s):
WU Li-fang1;  ZHANG Lin1;  ZHANG Jie2;  WANG Zhan-jun1
（1. Department of Otolaryngology Head and Neck Surgery, the fifth People’s Hospital of Datong, Shanxi Datong 037000, China;2. Department of Otolaryngology, Medical College of Datong University, Shanxi Datong 037000, China）
关键词:
变应性鼻炎; 正五聚蛋白 3; 脂氧素 A4
分类号:
R765.21；R392.11
DOI:
10.3969/j.issn.1671-7414.2023.02.026
文献标志码:
A
摘要:
目的 分析变应性鼻炎（allergic rhinitis，AR）患者血清正五聚蛋白 3（pentraxin 3，PTX3）和脂氧素 A4（lipoxin A4，LXA4）水平及临床意义。方法 选取 2019年 6月～2022年 6月 80例大同市第五人民医院已确诊收治的 AR患者为 AR组，其中轻度 AR 27例、中度 AR 23例和重度 AR 30例。同期在该院体检的健康者 90例为对照组。酶联免疫吸附法（enzyme linked immunosorbent assay，ELISA）检测血清中 PTX3和 LXA4水平；用 Pearson法分析 AR患者血清 PTX3与 LXA4表达相关性，以及 PTX3，LXA4与过敏性鼻炎评分量表（score for allergic rhinitis，SFAR）评分的相关性；绘制受试者工作特征（receiver operating characteristic，ROC）曲线分析 PTX3，LXA4对 AR的诊断价值；采用 logistic回归分析中重度 AR发生的影响因素。结果 AR组血清 PTX3（22.62±2.57ng/ml）和 LXA4（60.81±6.33nmol/L）水平明显高于对照组（17.81±2.56ng/ml，54.83±6.24nmol/L），差异具有统计学意义（t=12.205，6.195，均 P<0.001）。中度、重度 AR组血清 PTX3（21.13±1.78，26.42±3.57ng/ml）和 LXA4水平（59.87±6.34，66.54±7.13nmol/L）明显高于轻度 AR组（19.69±2.14ng/ml，55.23±5.42nmol/L），差异具有统计学意义（F=76.181，22.762，均 P<0.001）。PTX3和 LXA4高表达组病情程度为中、重度， SFAR评分≥ 7分，IgE阳性患者所占比例高于病情程度为轻度、 SFAR评分 <7分，IgE阴性患者，差异具有统计学意义（χ2=6.265，4.059，5.485，10.908，8.038，11.077，均 P＜ 0.05）；Pearson相关性分析显示， AR患者血清 PTX3与 LXA4表达水平呈显著正相关（r=0.592，P<0.001），PTX3，LXA4与 SFAR评分呈显著正相关（r=0.617，0.843，均 P<0.001）。ROC曲线分析显示，血清 PTX3诊断 AR发生的 AUC，敏感度和特异度分别为 0.916（95%CI=0.863～0.953），80.00%，90.00%；LXA4诊断 AR发生的 AUC，敏感度和特异度分别为 0.705（95%CI=0.630～0.772），86.25%，46.67%；两者联合诊断的 AUC，敏感度和特异度分别为 0.938（95%CI=0.890～0.969）93.75%，90.00%。Logistic回归分析表明， SFAR评分（OR=1.617，95%CI=1.256～2.082）、PTX3（OR=1.537， 95%CI=1.152～2.050）和 LXA4（OR=1.463，95%CI=1.125～1.902）均是中重度 AR发生的影响因素（Wald χ2=13.878，8.550，8.063，均 P＜ 0.05）。结论 PTX3和 LXA4在 AR患者血清中高表达，两者与 AR患者的临床病理特征和病情严重程度关系密切，同时其表达水平对 AR的诊断具有一定的价值。
Abstract:
Objective To analyze the levels and clinical significance of serum pentraxin 3 (PTX3) and lipoxin A4 (LXA4) in patients with allergic rhinitis (AR). Methods From June 2019 to June 2022, 80 AR patients diagnosed and admitted to the fifth People’s Hospital of Datong were regarded as AR group, including 27 cases of mild AR, 23 cases of moderate AR, and 30 cases of severe AR. Meantime, 90 healthy people who underwent physical examination in the hospital were the control group. The levels of PTX3 and LXA4 in serum were detected by ELISA . Pearson method was used to analyze the correlation between the expressions of serum PTX3 and LXA4 in AR patients, and the correlation between PTX3, LXA4 and the score for allergic rhinitis (SFAR) . ROC curve was used to analyze the diagnostic value of PTX3 and LXA4 for AR and logistic regression was used to analyze the influencing factors of moderate to severe AR. Results The levels of serum PTX3(22.62±2.57 ng/ml) and LXA4 (60.81±6.33 nmol/L) in AR group were significantly higher than those in control group (17.81±2.56 ng/ml, 54.83±6.24 nmol/L), and the differences were statistically significant (t=12.205, 6.195, all P<0.001). The levels of serum PTX3 (21.13±1.78, 26.42±3.57 ng/ml) and LXA4 (59.87±6.34, 66.54±7.13 nmol/L) in moderate and severe AR groups were significantly higher than those in mild AR group (19.69±2.14 ng/ml, 55.23±5.42nmol/L), and the difference was statistically significant (F=76.181, 22.762, all P<0.001). The proportions of patients with moderate to severe disease, SFAR score ≥ 7 points, and IgE positive in PTX3 and LXA4 high expression group were greatly higher than those in patients with mild disease, SFAR score < 7 points, and IgE negative (χ2=6.265, 4.059，5.485, 10.908, 8.038, 11.077, all P<0.05). Pearson correlation analysis showed that the expression levels of serum PTX3 and LXA4 in AR patients were greatly positively correlated (r=0.592, P<0.001), and PTX3, LXA4 and SFAR scores were greatly positively correlated (r=0.617, 0.843 , all P<0.001). The ROC curve results showed that the AUC of serum PTX3 in the diagnosis of AR was 0.916 (95%CI=0.863～0.953), the sensitivity and the specificity was 80.00%, 90.00%, respectively. The AUC of LXA4 in the diagnosis of AR was 0.705 (95%CI=0.630～0.772), the sensitivity and the specificity was 86.25%, 46.67% . The AUC of the combined diagnosis was 0.938 (95%CI=0.890～0.969), the sensitivity and the specificity was 93.75%, 90.00%. Logistic regression analysis showed that SFAR score（OR=1.617, 95%CI=1.256～2.082）, PTX3（OR=1.537, 95%CI=1.152～2.050）and LXA4（OR=1.463, 95%CI=1.125～1.902）were all influencing factors of moderate to severe AR (Wald χ2=13.878, 8.550, 8.063, all P<0.05). Conclusion PTX3 and LXA4 are highly expressed in the serum of AR patients, and they are closely related to the clinicopathological characteristics and disease severity of AR patients. The expression levels of PTX3 and LXA4 have certain value in the diagnosis of AR.

参考文献/References:

[1] SCHULER IV C F, MONTEJO J M. Allergic rhinitis in children and adolescents[J]. Pediatr Clin North Am, 2019, 66(5):981-993.
[2] 孙序元 , 宋维 , 钟玲玲 . 813例儿童变应性鼻炎的临床特征及影响因素分析 [J]. 中国妇幼保健 , 2019, 34(22)：5210-5212. SUN Xuyuan, SONG Wei, ZHONG Lingling. Clinical characteristics and influencing factors of 813 children with allergic rhinitis[J]. Maternal & Child Health Care of China, 2019, 34(22): 5210-5212.
[3] 陈永照 , 钟丽花 , 杨辉 , 等 . 血清乳酸和正五聚蛋白 3及单核细胞趋化蛋白 1联合检测对新生儿早发型败血症诊断及预后评估的价值 [J]. 中华医院感染学杂志 , 2022, 32(8): 1215-1219. CHEN Yongzhao, ZHONG Lihua, YANG Hui, et al. Value of joint detection of serum lactic acid, pentrax-in-3 and monocyte chemotactic protein-1 in diagnosis of neonatal early-onset sepsis and assessment of prog-nosis[J]. Chinese Journal of Nosocomiology, 2022, 32(8): 1215-1219.
[4] WU Xunyao, LI Ketian, YANG Huaxia, et al. Comple-ment C1q synergizes with PTX3 in promoting NLRP3 inflammasome over-activation and pyroptosis in rheu-matoid arthritis[J]. Journal of Autoimmunity, 2020, 106: 102336.
[5] LI An, ZHAO Fangfang, YANG Ting, et al. PTX3/ TWIST1 feedback loop modulates Lipopolysaccharide-induced inflammation via PI3K/Akt signaling pathway[J]. Journal of Interferon & Cytokine Research, 2022, 42(4): 161-169.
[6] CHEN Chaojin, QIU Rongzong, YANG Jing, et al. Lipoxin A4 restores septic renal function via blocking crosstalk between inflammation and premature senescence[J]. Frontiers in Immunology, 2021, 12: 637753.
[7] YE Wen, ZHENG Chenlei, YU Dinglai, et al. Lipoxin A4 ameliorates acute pancreatitis-associated acute lung injury through the antioxidative and anti-inflammatory effects of the Nrf2 pathway[J]. Oxidative Medicine and Cellular Longevity, 2019, 2019: 2197017.
[8] ZHU Xiaojia, PENG Xudong, LIN Jing, et al. Lipoxin A4 activates ALX/FPR2 to attenuate inflammation in aspergillus fumigatus keratitis[J]. International Immunopharmacology, 2021, 96: 107785.
[9] 中华耳鼻咽喉头颈外科杂志编辑委员会鼻科组 , 中华医学会耳鼻咽喉头颈外科学分会鼻科学组 . 中国变应性鼻炎诊断和治疗指南 (2022年 , 修订版 )[J]. 中华耳鼻咽喉头颈外科杂志 , 2022, 57(2) : 106-109. Subspecialty Group of Rhinology, Editorial Board of Chinese Journal of Otorhinolaryngology Head and Neck Surgery, Subspecialty Groups of Rhinology and Pediatrics, Society of Otorhinolaryngology Head and Neck Surgery, Chinese Medical Association. Chinese guideline for diagnosis and treatment of allergic rhinitis (2022, revision) [J]. Chinese Journal Otorhinolaryngology Head and Neck Surgery, 2022, 57(2): 106-109.
[10] KLIMEK L, SPERL A, BECKER S, et al. Current therapeutical strategies for allergic rhinitis[J]. Expert Opinion on Pharmacotherapy, 2019, 20(1): 83-89.
[11] BOUSQUET J, ANTO J M, BACHERT C, et al. Aller-gic rhinitis[J]. Nature Reviews Disease Primers, 2020, 6(1): 95.
[12] LIU H C, LIAO Y, LIU C Q. MiR-487b mitigates allergic rhinitis through inhibition of the IL-33/ST2 signaling pathway[J]. European Review for Medical and Pharmacological Sciences, 2018, 22(23): 8076-8083.
[13]林李娜 , 何微 , 刘国栋 , 等 . 变应性鼻炎患儿血清 IL-33, ST2水平及 IL-33基因 rs3939286 G/A位点多态性与疾病程度相关性分析 [J]. 现代检验医学杂志 , 2022, 37(3): 127-131. LIN Lina, HE Wei, LIU Guodong, et al. Correlation analysis between the levels of IL-33, ST2 and polymorphism of IL-33 gene rs3939286 G/A and different degree of allergic rhinitis in children[J]. Journal of Modern Laboratory Medicine, 2022, 37(3): 127-131.
[14] COX Linda. Approach to patients with allergic rhinitis: testing and treatment[J]. The Medical Clinics of North America, 2020, 104(1): 77-94.
[15] 杨心怡 , 孙海勇 , 冀德君 , 等 . BCAP在变应性鼻炎患者外周血中的差异性表达 [J]. 中国中西医结合耳鼻咽喉科杂志 , 2021, 29(3)：184-187. YANG Xinyi, SUN Haiyong, JI Dejun, et al. Differen-tial expression of BCAP gene in patients with allergic rhinitis[J]. Chinese Journal of Otorhinolaryngology in Integrative Medicine, 2021, 29(3): 184-187.
[16] RAMIREZ G A, ROVERE-QUERINI P, BLASI M, et al. PTX3 intercepts vascular inflammation in systemic immune-mediated diseases [J]. Frontiers in Immunology, 2019, 10: 1135.
[17] TSAI Y J, HAO C Y, CHEN C L, et al. Expression of long pentraxin 3 in human nasal mucosa fibroblasts, tissues, and secretions of chronic rhinosinusitis without nasal polyps[J]. Journal of Molecular Medicine (Berlin, Germany), 2020, 98(5): 673-689.
[18] 熊洪斌 , 杨茜 , 石润杰 . 外周血 CCL26, CCR3表达与变应性鼻炎患者疾病严重程度的关系 [J]. 海南医学 , 2022, 33(12): 1515-1519. XIONG Hongbin, YANG Qian, SHI Runjie. Relationship between the expression levels of peripheral blood CCL26, CCR3 and the severity of allergic rhinitis[J]. Hainan Medical Journal, 2022, 33(12): 1515-1519.
[19] AWAN N U, SOHAIL S K, NAUMERI F, et al. Asso-ciation of serum vitamin D and immunoglobulin E lev-els with severity of allergic rhinitis[J]. Cureus, 2021, 13(1): e12911.
[20] 梁龙威 , 冯景焜 . 脂氧素 A4与冠心病相关性的研究进展 [J]. 海南医学 , 2020, 31(7)：910-913. LIANG Longwei, FENG Jingkun. Research progress on the relationship between Lipoxin A4 and coronary heart disease[J]. Hainan Medical Journal, 2020, 31(7): 910-913.
[21] ZHU Jinjin, YU Binyuan, FU Changchang, et al. LXA4 protects against hypoxic-ischemic damage in neonatal rats by reducing the inflammatory response via the IκB/NF-κB pathway[J]. International Immunopharmacology, 2020, 89(Pt B): 107095.
[22] 朱真真 , 王威清 , 韩晋博 , 等 . 特异性促炎症消退介质在嗜酸粒细胞性与非嗜酸粒细胞性慢性鼻窦炎伴鼻息肉中的含量差异 [J]. 中华耳鼻咽喉头颈外科杂志 , 2021, 56(10): 1073-1079. ZHU Zhenzhen, WANG Weiqing, HAN Jinbo, et al. Different concentrations of specialized pro-resolving mediators in eosinophilic and non-eosinophilic chronic rhinosinusitis with nasal polyps[J]. Chinese Journal of Otorhinolaryngology Head and Neck Surgery, 2021, 56(10): 1073-1079.
[23] 张炳煌 , 朱旭丽 , 高静 . 脂氧素 A4及脂氧素 A4受体在慢性鼻 -鼻窦炎上皮 -间质转化中的作用 [J]. 中国耳鼻咽喉头颈外科 , 2021, 28(6)：370-374. ZHANG Binghuang, ZHU Xuli, GAO Jing. The effect of lipoxin A4 and lipoxin A4 receptor in the epithelial-mesenchymal transition of chronic rhinosinusitis[J]. Chinese Archives of Otolaryngology-Head and Neck Surgery, 2021, 28(6): 370-374.

相似文献/References:

[1]林李娜a,何　微a,刘国栋b,等.变应性鼻炎患儿血清IL-33，ST2水平及IL-33基因rs3939286G/A位点多态性与疾病程度相关性分析[J].现代检验医学杂志,2022,37(03):127.[doi:10.3969/j.issn.1671-7414.2021.03.027]
　LIN Li-naa,HE Weia,LIU Guo-dongb,et al.Correlation Analysis between the Levels of IL-33,ST2 and Polymorphism of IL-33 Gene rs3939286 G/A and Different Degree of Allergic Rhinitis in Children[J].Journal of Modern Laboratory Medicine,2022,37(02):127.[doi:10.3969/j.issn.1671-7414.2021.03.027]

备注/Memo

备注/Memo:
收稿日期：2022-10-09修回日期：2022-11-07
作者简介：吴莉芳（1974-），女，大学本科，副主任医师，研究方向：耳鼻咽喉头颈外科， E-mail：wulifang1974f@163.com。

更新日期/Last Update: 2023-03-15