[1]邢俊杰,盛永亮,张浩然,等.非肌层浸润性膀胱癌TURBT 治疗患者血清UBC1 和DJ-1 表达水平及其对预后预测价值研究[J].现代检验医学杂志,2023,38(03):29-34.[doi:10.3969/j.issn.1671-7414.2023.03.006]
　XING Jun-jie,SHENG Yong-liang,ZHANG Hao-ran,et al.Study on Serum UBC1, DJ-1 Expression Levels and Their Prognostic Value in Patients with Non Muscular Invasive Bladder Cancer Treated with TURBT[J].Journal of Modern Laboratory Medicine,2023,38(03):29-34.[doi:10.3969/j.issn.1671-7414.2023.03.006]
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非肌层浸润性膀胱癌TURBT 治疗患者血清UBC1 和DJ-1 表达水平及其对预后预测价值研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第38卷
期数:
2023年03期
页码:
29-34
栏目:
论著
出版日期:
2023-05-15

文章信息/Info

Title:
Study on Serum UBC1, DJ-1 Expression Levels and Their Prognostic Value in Patients with Non Muscular Invasive Bladder Cancer Treated with TURBT
文章编号:
1671-7414（2023）03-029-06
作者:
邢俊杰; 盛永亮; 张浩然; 刘春辉; 李志军
（河南科技大学第一附属医院泌尿外二科，河南洛阳 471000）
Author(s):
XING Jun-jie; SHENG Yong-liang; ZHANG Hao-ran; LIU Chun-hui; LI Zhi-jun
（Department II of Urology, the First Affiliated Hospital of Henan University of Science and Technology, Henan Luoyang 471000, China）
关键词:
非肌层浸润性膀胱癌; 经尿道膀胱肿瘤电切术; 长链非编码RNA- 尿路上皮癌相关基因1; 帕金森病相关蛋白-1
分类号:
R737.14；R730.43
DOI:
10.3969/j.issn.1671-7414.2023.03.006
文献标志码:
A
摘要:
目的 研究非肌层浸润性膀胱癌(non-muscle invasive bladder cancer，NMIBC) 患者血清长链非编码RNA 尿路上皮癌相关基因1[（LncRNA）upregulated in bladder cancer 1, UBC1]、帕金森病相关蛋白-1（DJ-1）的表达情况，分析两者对NMIBC 患者预后的影响。方法 选择2018 年2 月～ 2019 年2 月于河南科技大学第一附属医院接受经尿道膀胱肿瘤电切（TURBT）治疗的120 例NMIBC 患者为研究对象（NMIBC 组），以同期健康体检的60 例健康人群为对照组。应用实时荧光定量PCR 检测各组血清UBC1 水平。应用酶联免疫吸附实验检测各组血清DJ-1 水平。比较不同临床病理特征NMIBC 患者血清UBC1 和DJ-1 表达差异。Kaplan-Meier 生存曲线分析血清UBC1 和DJ-1 表达对NMIBC 患者无进展生存预后的影响。单因素及多因素COX 回归分析影响NMIBC 患者无进展生存预后的因素。结果 相比于对照组，NMIBC 组血清UBC1（4.19±0.48 vs 1.27±0.29）和DJ-1（8.62±3.60 ng/ml vs 4.31±1.07 ng/ml）水平升高，差异具有统计学意义（t=43.300，12.117，均P ＜ 0.05）。肿瘤T1 期、高级别NMIBC 患者血清中UBC1（5.21±0.56，5.11±0.53），DJ-1（11.28±3.98 ng/ml ，10.50±3.87 ng/ml）表达分别高于Ta/Tis 期、低级别患者（3.79±0.43，3.64±0.44；7.34±3.04ng/ml，7.49±3.23 ng/ml），差异具有统计学意义 (t=15.314, 5.966；16.393, 4.584，均P <0.05）。UBC1 高表达组和低表达组患者的平均无进展生存时间分别为28.17±3.68 个月和33.59±3.32 个月。UBC1 高表达组患者累积无进展生存时间低于UBC1 低表达组患者，差异具有统计学意义（Log-Rank test χ2=6.681，P ＜ 0.05）。DJ-1 高表达组和低表达组平均无进展生存时间分别为27.34±3.29 个月和34.27±3.54 个月。DJ-1 高表达组患者累积无进展生存时间低于DJ-1 低表达组患者，差异具有统计学意义（Log-Rank test χ2=11.262，P ＜ 0.05）。肿瘤分期T1 期（HR=1.613，95%CI=1.223 ～ 2.126）、肿瘤分级高级别（HR=1.917，95%CI=1.314 ～ 2.799），UBC1 高表达（HR=1.937，95%CI=1.229 ～ 2.745）和DJ-1 高表达（HR=1.738，95%CI=1.246 ～ 2.426）是影响NMIBC 患者无进展生存预后的独立危险因素。结论 NMIBC 患者血清UBC1 和DJ-1 表达升高，两者表达与肿瘤分期及肿瘤分级有关，是影响NMIBC患者无进展生存预后的独立因素。
Abstract:
Objective To study the expression of long-chain non coding RNA upregulated in bladder cancer 1(UBC1) and Parkinson’s disease associated protein-1 (DJ-1) in serum of patients with non-muscle invasive bladder cancer (NMIBC), and to analyze their effects on the prognosis of patients with NMIBC. Methods 120 NMIBC patients who received TURBT treatment in the First Affiliated Hospital of He’nan University of Science and Technology from February 2018 to February 2019 were selected as the NMIBC group, and 60 healthy people who received physical examination at the same time were selected as the control group. Serum UBC1 levels were detected by real-time fluorescence quantitative PCR. The serum DJ-1 level was detected by enzyme-linked immunosorbent assay. The difference of serum UBC1 and DJ-1 expression in NMIBC patients with different clinical and pathological characteristics were compared. Kaplan Meier survival analysis were used to analyze the influence of serum UBC1 and DJ-1 level on the prognosis of progression free survival in NMIBC patients. Univariate and multivariate COX regression analysis were used to analyze factors influencing the prognosis of progression free survival in NMIBC patients. Results Compared with the control group, the serum UBC1 in NMIBC group was significantly higher (1.27 ± 0.29 vs 4.19 ± 0.48), the serum DJ-1 was significantly higher (4.31 ± 1.07 ng/ml vs 8.62 ± 3.60 ng/ml), and the differences were statistically significant (t=43.300, 12.117, all P=0.000). The expression of UBC1 (5.21 ± 0.56 ，5.11 ± 0.53) and DJ-1 (11.28 ± 3.98 ng/ml ，10.50 ± 3.87 ng/ml) in the serum of patients with tumor T1 and high-grade NMIBC was higher than that of patients with tumor T1 and high-grade NMIBC（3.79±0.43，3.64±0.44；7.34±3.04ng/ml，7.49±3.23 ng/ml）, and the differences were statistically significant (t=15.314, 5.966；16.393, 4.584，all P<0.05). The mean progression free survival time of patients with high and low expression of UBC1 was 28.17 ± 3.68 months and 33.59 ± 3.32 months, respectively. Kaplan Meier survival analysis showed that the cumulative progression free survival of patients with high UBC1 expression was significantly lower than that of patients with low UBC1 expression (Log rank test χ2=6.681，P=0.000）.The mean survival time without progression in DJ-1 high expression group and low expression group was 27.34 ± 3.29 months and 34.27 ± 3.54 months, respectively. The cumulative progression free survival of patients with high DJ-1 expression was significantly lower than that of patients with low DJ-1 expression (Log rank test χ2=11.262，P=0.000）. Tumor stage T1 (HR=1.613, 95% CI=1.223 ～ 2.126), high tumor grade (HR=1.917, 95%CI=1.314 ～ 2.799), high expression of UBC1 (HR=1.937, 95%CI=1.229 ～ 2.745), and high expression of DJ-1 (HR=1.738, 95%CI=1.246 ～ 2.426) were independent risk factors affecting the prognosis of progression free survival in NMIBC patients. Conclusion The expression of UBC1 and DJ-1 in serum of NMIBC patients increased, which were related to tumor stage and tumor grade, and there are independent factors affecting the prognosis of progression free survival in NMIBC patients.

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备注/Memo

备注/Memo:
基金项目：河南省医学科技攻关计划项目（LHGJ20201267）。
作者简介：邢俊杰（1996-），男，硕士研究生，住院医师，研究方向：泌尿外科， E-mail：xingjunjie1015@163.com。
通讯作者：李志军（1965-），男，本科，主任医师，研究方向：泌尿外科，E-mail：lydoc@126.com。
更新日期/Last Update: 2023-05-15